|Publication number||US3261208 A|
|Publication date||Jul 19, 1966|
|Filing date||May 13, 1964|
|Priority date||May 13, 1964|
|Publication number||US 3261208 A, US 3261208A, US-A-3261208, US3261208 A, US3261208A|
|Inventors||Fisher Timothy L|
|Original Assignee||Fisher Timothy L|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (2), Referenced by (14), Classifications (8)|
|External Links: USPTO, USPTO Assignment, Espacenet|
July 19, 1966 T. L. FISHER 3,261,208
AUTOMATIC PIPETTE Filed May 13, 1964 2 Sheets-Sheet l Presfurc /fne Jen/917010 valve Vacuum /me INVENTOR. /7na/zy L. /T/sher' BY: m. 1,9m?
July 19, 1966 T. FISHER AUTOMATIC PIPETTE 2 Sheets-Sima?I 2 Filed May 15,. 1964 INVENTOR.
United States Patent O 3,261,208 AUTOMATIC PIPETTE Timothy L. Fisher, 863 W. Walnut St., Shamokin, Ia. Filed May 13, 1964, Ser. No. 367,259 1 Claim. (Cl. 73-425.6)
The invention described herein may be manufactured and used by or for the Government for governmental purposes, without the payment to me of any royalty thereon.
Pipettes are used in varying ways. As one example, a pipette may be used to extract a sample of a uid from a test tube and to then insert the sample into another test tube or container.
The quantity of the `sample transferred should be uniform in sample-aftepsarnple for many types of experimental work. Also, large numbers of samples can be transferred simultaneously with properly designed equipment, thus saving valuable and expensive scientist and technician time.
The present invention improves pipettes and pipettesample transferring techniques.
In the drawings:
FIG. 1 is a perspective View of a setup embodying the present invention;
FIG. 2 is a detail of one pipette and the associated vaeuum and pressure lines;
FIG. 3 is a detail of a vacuum or pressure manifold;
FIG. 4 is -a partial cross-sectional view of a pipette; and
FIG. 5 is a detail of the pipette of FIG. 4.
In detail, FIG. 1 illustrates a complete setup say of fty pipettes 28 mounted on a vertically movable rack 10 supported by posts 12. A box 14 is placed beneath rack 10 and may contain fty test tubes, one mounted beneath each pipette.
Rack carries five pairs of vacuum and pressure manifolds 36, 30 respectively each manifold having connections for ten pipettes. Each vacuum manifold is connected, through conduit 15, to a vacuum line 16 to which vacuum may be .applied by use of solenoid valve 18 and switch 20. Each pressure manifold is connected, through conduit 21, to a pressure line 22 to which pressure may be applied by ues of solenoid valve 24 and switch 26.
Vacuum line 15, 16 connects with a plurality of vacuum manifolds 36 (FIG. 2) and pressure line 21, 22 connects with a plurailty of pressure manifolds 30 so that vacuum or pressure may be applied alternatively to the pipettes 28.
FIGS. 4 and 5 show glass pipette 28 having neck 42 communicating with conduit 44 to which vacuum is applied. Hollow oat 46 is limited in downward movement by stops 48. Float 46 has a seat 50 cooperating with seat 52 to form a valve. The pipette has a xed volume in the area 54 below float 46 and valve 50, 52. Means could be provided to vary this volume, or pipettes having different capacities could be used interchangeably.
Operation FIG. 1 illustrates a plurailty of say fifty pipettes for extracting samples of liquid from lan equal number of test tubes, say fty for example. If there are only forty test tubes then pinch-clamps 34, 40 (FIG. 2) may be closed to ICC collapse and close flexible ilnes 32, 38 for the ten pipettes not to be used.
Rack 10 is lowered to bring the plurality of pipettes into communication with the liquid in the test tubes. Switch 26 is closed to open solenoid valve 24 and thereby apply a partial vacuum to manifolds 36 and pipettes 28 via 38, 44, 42. The partial vacuum causes liquid to rise in the pipettes into area 54 until float 46 is buoyed up by the liquid and closes valve 50, 52. If any liquid escapes above valve 59, 52, then the vacuum applied to extension 42 causes the excess to be drawn off, thereby assuring that only a precisely measured quantity remains in the pipette.
Rack 10 is then raised and the box of test tubes is removed from therebeneath. `Other containers which are to receive the samples are placed below pipettes 2S. Pressure is applied in line 22 and pressure manifolds 30 by opening valve 24 and the liquid in the pipettes is expelled.
From the foregoing it is seen that this invention can be operated rapidly to withdraw precisely measured quantities of liquid from one container, or from one set of containers, and to deposit such precisely measured quantities into another or other containers. The operator merely presses button 26 and all the pipettes are automatically lled with precisely measured quanti-ties. The operator merely presses button 20 and the pipettes discharge precisely measured quantities.
a rack mounted for vertical movement on supporting means;
a plurality of vacuum and pressure manifolds mounted on the rack;
a plurality of pipettes connected to the vacuum and pressure manifolds;
Vacuum and pressure lines connected to the vacuum and pressure manifolds;
means to control application of vacuum or pressure alternatively to the lines to thereby apply vacuum or pressure at will to the pipettes; each pipette comprislng:
a section with a cavity of a desired volume; a float-operated valve in communication with the cavity; vacuum line connection means; pressure line connection means; the vacuum line connection means communicating with the interior of the pipette at a point immediately above the valve such that any liquid which might get past the valve will be withdrawn from the pipette thereby .assuring substantially equal volume even if the valve fails to operate perfectly.
References Cited bythe Examiner UNITED STATES PATENTS 8/1964 Des Hons.
|Cited Patent||Filing date||Publication date||Applicant||Title|
|US3143393 *||Jun 13, 1960||Aug 4, 1964||Luc Donald De Seguin Des Hons||Apparatus for automatically performing chemical operations and similar or related operations|
|DE116797C *||Title not available|
|Citing Patent||Filing date||Publication date||Applicant||Title|
|US3444742 *||Dec 6, 1965||May 20, 1969||Univ Of Kentucky Research Foun||Multiple-unit pipetting assembly and pipette for use therein|
|US3572552 *||Jul 25, 1969||Mar 30, 1971||Perry W Guinn||Diaphragm dispenser|
|US3738530 *||Jul 26, 1971||Jun 12, 1973||Fine H||Seeder device|
|US3827286 *||Jul 21, 1972||Aug 6, 1974||Gelman Instr Co||Device for blood sedimentation rate estimation|
|US4493896 *||Oct 14, 1982||Jan 15, 1985||Bio-Rad Laboratories, Inc.||Dual chamber microplate washer|
|US4496657 *||Oct 14, 1982||Jan 29, 1985||Scripps Clinic And Research Foundation||Microplate washer|
|US4649967 *||Aug 29, 1985||Mar 17, 1987||University Of Cincinnati||Multiple efflux apparatuses for transferring fluid|
|US5348606 *||Sep 23, 1992||Sep 20, 1994||Hanaway Richard W||Method of making a multiple pipette sampler system|
|US5865224 *||Dec 20, 1996||Feb 2, 1999||Life Technologies, Inc.||Method and apparatus for automated dispensing|
|US6039211 *||Sep 22, 1998||Mar 21, 2000||Glaxo Wellcome Inc.||Position triggered dispenser and methods|
|US6044876 *||Jan 7, 1999||Apr 4, 2000||Life Technologies||Method and apparatus for automated dispensing|
|EP0013614A1 *||Jan 7, 1980||Jul 23, 1980||William Thomas Dennis Bates||Sample tube|
|WO1980002706A1 *||May 22, 1980||Dec 11, 1980||Uresil Co||Apparatus and method for introducing fluid into and removing fluid from a living subject|
|WO2000017093A1 *||Sep 17, 1999||Mar 30, 2000||Glaxo Group Limited||Position triggered dispenser and methods|
|U.S. Classification||73/863.32, 422/922, 222/263, 73/864.15, 141/234|