Search Images Maps Play YouTube News Gmail Drive More »
Sign in
Screen reader users: click this link for accessible mode. Accessible mode has the same essential features but works better with your reader.


  1. Advanced Patent Search
Publication numberUS3322114 A
Publication typeGrant
Publication dateMay 30, 1967
Filing dateJul 1, 1964
Priority dateJul 1, 1964
Publication numberUS 3322114 A, US 3322114A, US-A-3322114, US3322114 A, US3322114A
InventorsPortnoy Joseph, George S Warner
Original AssigneeHynson Westcott & Dunning Inc
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Apparatus for securing a sample of blood plasma for testing
US 3322114 A
Abstract  available in
Previous page
Next page
Claims  available in
Description  (OCR text may contain errors)


assignors to Hynson, Westcott & Dunning, Incorporated, a corporation of Maryland Filed July 1, 1964, Ser. No. 379,498

3 Claims. (Cl. 128-2) ABSTRACT OF THE DISCLOSURE The invention resides in a composition of an anticoagulant and a lectin in a suitable container such as a disposable syringe to which a sample of blood may be added whereby the blood cells are coagulated by the lectin while clotting of the blood is prevented by the anticoagulant and the blood plasma is separated.

Samples of blood plasma of humans and various animals are required for carrying out a variety of tests such as those described in US. Patent No. 3,074,853.

The tests described in said patent require only a small amount of plasma such as the quantity available from only a few drops of blood that readily may be obtained e.g. by puncturing a finger of a person with a lancet but for some tests or for carrying out a plurality of tests more plasma frequently is needed and it is an object of our invention to provide apparatus that is adapted to obtain and deliver from a few drops up to several cubic centimeters of blood plasma.

The invention resides primarily in a hypodermic needle of a suitable diameter and length for extracting blood from a person or animal secured to the neck of a bottle or container of a suitable transparent or at least translucent plastic such as polyethylene, said bottle containing an anticoagulant such as heparin or one of the salts of ethylenediaminetetraacetic acid and a lectin or phytoagglutinin material such as that extracted from Phaseolzzs commzmis or Phaseolus vulgaris. For example, in the separation of plasma from swine blood said bottle may contain 1 mg. of lectin and 1 mg. ethylenediaminetetraacetic acid.

In the preferred form of our invention the needle is secured to a hub which in turn is detachably secured to the bottle and the apparatus comprises also a dispensing nozzle adapted to be applied to the bottle after the needle has been detached, said dispensing nozzle optionally containing a filter.

The invention is illustrated in the accompanying drawings in which:

FIG. 1 is an exploded side elevational view of the bottle containing a mass of a mixture of anticoagulant and lectin, the needle and its hub and a protective cap for the needle and FIG. 2 is a sectional view of the dispensing nozzle provided with a filter.

Referring to the drawings 1 is the needle protective cap, 2 is the hypodermic needle, 3 is the needle hub, 4 the bottle, 5 is a mass of anticoagulant and lectin and 6 is the neck of the bottle. The needle cap, needle and hub are all well known and need not be described. The bottle 4 is formed of a flexible and elastic or resilient plastic such as polyethylene, the wall of the bottle being of such thickness that it retains its normal shape e.g. cylindrical but is readily deformable i.e. collapsed by the pressure of the thumb and finger of the operator and will return to its original shape when the pressure is released. The wall of the bottle should be sufficiently resilient to create a slight suction and thus to draw blood through the needle into the bottle but not so resilient as to prevent collapsing 3,322,114 Patented May 30, 1967 "ice by the pressure of the fingers or to create sutficient suction on the vein from which blood is being drawn to cause collapse of the vein. We have found that a bottle the wall of which is sufficiently rigid to maintain its shape and requires only gentle pressure of the thumb and finger to collapse it will, due to its resilient tendency to return to its original shape when the pressure is relaxed, create sufiicient suction to draw blood from the vein of a human or animal but not sufiicient ordinarily to collapse the vein.

Anticoagulants and phytoagglutinins or lectins are well known and the kinds and quantities thereof required to prevent clotting (anticoagulant) and to cause agglomeration of the blood cells (lectin) of the kind and quantity of the blood sample are well known and need not be discussed. For a disclosure of lectins and their uses We refer to the book entitled Introduction to Immunochemical Specificity by William C. Boyd, John Wiley & Sons, 1962.

The anticoagulant and lectin may be introduced into the bottle 4 either separately or mixed with each other, in either liquid or solid form, and either through the neck of the bottle or through the bottom end of the bottle before it is closed and sealed.

The dispensing nozzle 7 may be of the same shape'as the hub 3 of the needle, and as appears in FIG. 2, contains a filter 8 and has a short neck 9 which serves to deliver a drop of plasma of a suitable size for the spot .test described in Patent No. 3,074,853. The filter 8 may be formed of a variety of filter materials such as pressed glass fiber, pressed nitrocellulose fiber, non-absorbent cotton, filter paper or the like and may be formed by packing loose fiber into the conical end of the nozzle or by cutting a disc from a filter in sheet form and inserting it in the conical space. The filter should be such as will remove solid particles such as blood cells from the plasma but not so difiicultly pervious that the pressure required to force the plasma through the filter will effect hemolysis of the blood cells. It is within the scope of our invention to impregnate the filter with lectin 0r anticoagulant or both to render it more effective i.e. to assist in maintaining the plasma uncoagulated and in agglomerating the blood cells and preventing their passage through the filter. It is also within the scope of our invention to omit the filter.

The apparatus is used as follows.

The bottle 4 containing the lectin and anticoagulant is partially collapsed by the pressure of the thumb and finger and the needle 2 which is attached by its hub 3 to the neck 6 of the bottle is inserted into a vein of a person or animal and the pressure on the bottle released whereupon blood flows through the needle into the bottle and the bottle returns to its normal uncompressed shape. The needle is then withdrawn from the vein and the contents of the bottle shaken to effect mixing of the blood with the lectin and anticoagulant and then allowed to stand whereupon.

the agglomerated blood cells separate and settle to the bottom of the bottle leaving a supernatant layer of clear plasma. By careful manipulation some of the clear plasma generally can be discharged through the needle or the needle may be removed and the plasma discharged through the neck 6. However it is preferred to replace the needle by the dispensing nozzle 7 and to discharge the plasma from the bottle through the filter 8. If preferred the neck of the plastic bottle can be cut-off thereby forming a small test tube from which clear plasma can be removed with a capillary tube.

The whole apparatus is inexpensive and disposable and is not intended for reuse.

We claim:

1. A device for drawing a sample of blood and separating plasma therefrom comprising a bottle having a transparent or translucent, flexible and resilient side wall capable of being collapsed by the pressure exerted by the thumb and finger of the operator and of returning to its normal shape when said pressure is released, a hypodermic needle attached to said bottle for aspirating blood from a vein of a person or animal into said bottle and an anti coagulant and a lectin in said bottle of a kind and in quantity suflicient to prevent clotting and to cause agglomeration of the cells of the kind and quantity of blood to be delivered into said bottle and to cause the separation of plasma from the collected blood.

2. A device for drawing a sample of blood and sepjarating plasma therefrom as defined in claim 1 in which said dispensing nozzle contains a filter.-

References Cited UNITED STATES PATENTS 2,722,257 11/1955 Lockhart 5. 128-216 X 2,982,286 5/1961 Welch 128216 3,045,494 7/ 1962 Gerarde 128216 X 3,089,489 5/1963 Dunmire 128-216 3,181,529 5/1965 Wilburn 1282 3,192,925 7/1965 Cunningham 128216 3,224,434 12/ 1965 Molomut 1282 RTCHARD A. GAUDET, Primary Examiner. V

S. BROD'ER, Assistant Examiner.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US2722257 *Feb 12, 1953Nov 1, 1955Compule CorpSampling tube equipment
US2982286 *Aug 3, 1956May 2, 1961Baxter Laboratories IncBlood collection apparatus
US3045494 *Mar 13, 1958Jul 24, 1962Gerarde Horace WilliamMethod of providing for blood count and pipette and assembly for use therein
US3089489 *Apr 27, 1959May 14, 1963Dunmire HannahAspirating type hypodermic syringes
US3181529 *Jul 30, 1962May 4, 1965Wilburn Edgar HValved body-fluid sampling tubes
US3192925 *Aug 25, 1961Jul 6, 1965Cunningham James RobertDisposable syringe device
US3224434 *Nov 6, 1962Dec 21, 1965Waldemar Medical Res FoundatioCell collector
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US3424148 *May 10, 1965Jan 28, 1969Jacques BlometDevice for taking blood samples
US3460529 *Jun 30, 1965Aug 12, 1969Leucci GinoSterile device for extracting urine samples and the like and package for same
US3481477 *Mar 2, 1965Dec 2, 1969Andrew F FarrApparatus for filtering out clear liquid from suspended solids
US3513829 *Aug 11, 1967May 26, 1970Brunswick CorpFluid collecting structure
US3518804 *Nov 1, 1966Jul 7, 1970Horace W GerardePipette assembly having precise quantity stabilized reagent in liquid form and method of preparing same
US3539300 *Oct 23, 1967Nov 10, 1970Schering CorpBody fluid collector and separator having improved flow rate
US3596652 *Oct 14, 1968Aug 3, 1971Bio Science LaborFluid separatory device
US3640267 *Dec 15, 1969Feb 8, 1972Damon CorpClinical sample container
US3718133 *Jan 12, 1971Feb 27, 1973Damon CorpContainer unit for liquid samples
US3800780 *Feb 23, 1972Apr 2, 1974Angelika ElliottVacuum indicator
US3809068 *Feb 16, 1973May 7, 1974Damon CorpContainer unit for liquid sample
US3817239 *May 12, 1972Jun 18, 1974Kuntz DUrine monitor
US3951798 *Feb 25, 1974Apr 20, 1976Glasrock Products, Inc.Container filter combination
US3978846 *Jan 2, 1975Sep 7, 1976Bailey Donald LSyringe for taking blood samples
US4014653 *Dec 26, 1974Mar 29, 1977Denver Chemical Manufacturing CompanyMicro-filter
US4014797 *May 20, 1975Mar 29, 1977Burron Medical Products, Inc.Intravenous injection apparatus and needle adapter with filter and method of making same
US4172448 *Jul 25, 1977Oct 30, 1979Sherwood Medical Industries Inc.Fluid sampling device
US4180060 *Aug 8, 1977Dec 25, 1979Boehringer Mannheim GmbhDevice for staining biological materials
US4227527 *Oct 23, 1978Oct 14, 1980Baxter Travenol Laboratories, Inc.Sterile air vent
US4259187 *Jun 7, 1979Mar 31, 1981Baxter Travenol Laboratories, Inc.Intravenous fluid filter
US4266558 *Apr 2, 1979May 12, 1981American Hospital Supply CorporationMethod of collecting and dispensing a blood sample
US4266559 *Apr 2, 1979May 12, 1981American Hospital Supply CorporationBlood sampler
US4298011 *Sep 7, 1979Nov 3, 1981Mangurten Henry HBlood sample collector
US4511351 *May 14, 1984Apr 16, 1985Alza CorporationParenteral delivery system utilizing a hollow fiber cellular unit
US4511352 *May 14, 1984Apr 16, 1985Alza CorporationParenteral delivery system with in-line container
US4552556 *Jan 4, 1985Nov 12, 1985Alza CorporationParenteral controlled therapy
US4579553 *Jan 7, 1985Apr 1, 1986Alza CorporationParenteral controlled therapy
US4583981 *Jan 7, 1985Apr 22, 1986Alza CorporationParenteral controlled therapy, using a porous matrix with parenteral agent
US4586922 *Feb 15, 1985May 6, 1986Alza CorporationIntravenous system for delivering a beneficial agent
US4596555 *Jan 28, 1985Jun 24, 1986Alza CorporationParenteral delivery system utilizing a hollow fiber cellular unit
US4740103 *Feb 15, 1985Apr 26, 1988Alza CorporationIntravenous system for delivering a beneficial agent
US4740197 *Feb 14, 1985Apr 26, 1988Alza CorporationIntravenous system for delivering a beneficial agent via polymer delivery
US4740198 *Feb 15, 1985Apr 26, 1988Alza CorporationMethod of administering intravenous drug using rate-controlled dosage form
US4740199 *Feb 14, 1985Apr 26, 1988Alza CorporationIntravenous system for delivering a beneficial agent
US4741734 *Feb 15, 1985May 3, 1988Alza CorporationReleasing means for adding agent using releasing means to IV fluid
US4871360 *Apr 21, 1986Oct 3, 1989Alza CorporationSystem for intravenous delivery of a beneficial drug at a regulated rates
US4980226 *Jul 10, 1985Dec 25, 1990Molnlycke AbFibrous absorption body for use in disposable articles
US5975313 *Feb 2, 1998Nov 2, 1999Sarstewdt Ag & Co.Blood-tube cap with coagulant additive
CN104107053A *Apr 15, 2014Oct 22, 2014贝克顿迪金森公司Biological fluid transfer device, biological fluid sampling system and biological fluid separation and testing system
EP0856280A2 *Jan 24, 1998Aug 5, 1998Sarstedt AG & Co.Device for taking blood
WO1983000281A1 *Jul 22, 1982Feb 3, 1983American Hospital Supply CorpVentable sample collection device
WO2014172234A1 *Apr 14, 2014Oct 23, 2014Becton, Dickinson And CompanyBiological fluid collection device and biological fluid separation and testing system
WO2014172241A1 *Apr 14, 2014Oct 23, 2014Becton, Dickinson And CompanyBiological fluid sampling transfer device and biological fluid separation and testing system
U.S. Classification600/578, D24/117, 600/580, 604/212, D24/224, 422/547
International ClassificationA61B5/15
Cooperative ClassificationA61B5/150389, A61B5/153, B01L2200/026, B01L2300/0681, A61B5/150519, A61B5/150717, A61B5/150587, A61B5/150755, B01L2300/0832, B01L3/502, A61B5/1405, B01L2400/0481, A61J1/05, A61B5/15003, A61B5/150099, A61M5/2425
European ClassificationA61B5/153, A61B5/15B18D6B, A61B5/15B18B2, A61B5/15B4B10, A61B5/15B2D, A61B5/15B18D12F, A61B5/15B26, A61B5/15B18B10D, B01L3/502, A61B5/14B