|Publication number||US3322114 A|
|Publication date||May 30, 1967|
|Filing date||Jul 1, 1964|
|Priority date||Jul 1, 1964|
|Publication number||US 3322114 A, US 3322114A, US-A-3322114, US3322114 A, US3322114A|
|Inventors||Portnoy Joseph, George S Warner|
|Original Assignee||Hynson Westcott & Dunning Inc|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (7), Referenced by (43), Classifications (33)|
|External Links: USPTO, USPTO Assignment, Espacenet|
May 30, 1967 omov ET AL APPARATUS FOR SECURING A SAMPLE OF BLOOD PLASMA FOR TESTING Filed July 1, 1964 I NVEN TOR WIk/V' ATTORNEYS Jaw/v POR77VOY BYEVMQWQLP United States Patent 3,322,114 APPARATUS FOR SECURING A SAMPLE OF BLOOD PLASMA FOR TESTING Joseph Portnoy and George S. Warner, Baltimore, Md.,
assignors to Hynson, Westcott & Dunning, Incorporated, a corporation of Maryland Filed July 1, 1964, Ser. No. 379,498
3 Claims. (Cl. 128-2) ABSTRACT OF THE DISCLOSURE The invention resides in a composition of an anticoagulant and a lectin in a suitable container such as a disposable syringe to which a sample of blood may be added whereby the blood cells are coagulated by the lectin while clotting of the blood is prevented by the anticoagulant and the blood plasma is separated.
Samples of blood plasma of humans and various animals are required for carrying out a variety of tests such as those described in US. Patent No. 3,074,853.
The tests described in said patent require only a small amount of plasma such as the quantity available from only a few drops of blood that readily may be obtained e.g. by puncturing a finger of a person with a lancet but for some tests or for carrying out a plurality of tests more plasma frequently is needed and it is an object of our invention to provide apparatus that is adapted to obtain and deliver from a few drops up to several cubic centimeters of blood plasma.
The invention resides primarily in a hypodermic needle of a suitable diameter and length for extracting blood from a person or animal secured to the neck of a bottle or container of a suitable transparent or at least translucent plastic such as polyethylene, said bottle containing an anticoagulant such as heparin or one of the salts of ethylenediaminetetraacetic acid and a lectin or phytoagglutinin material such as that extracted from Phaseolzzs commzmis or Phaseolus vulgaris. For example, in the separation of plasma from swine blood said bottle may contain 1 mg. of lectin and 1 mg. ethylenediaminetetraacetic acid.
In the preferred form of our invention the needle is secured to a hub which in turn is detachably secured to the bottle and the apparatus comprises also a dispensing nozzle adapted to be applied to the bottle after the needle has been detached, said dispensing nozzle optionally containing a filter.
The invention is illustrated in the accompanying drawings in which:
FIG. 1 is an exploded side elevational view of the bottle containing a mass of a mixture of anticoagulant and lectin, the needle and its hub and a protective cap for the needle and FIG. 2 is a sectional view of the dispensing nozzle provided with a filter.
Referring to the drawings 1 is the needle protective cap, 2 is the hypodermic needle, 3 is the needle hub, 4 the bottle, 5 is a mass of anticoagulant and lectin and 6 is the neck of the bottle. The needle cap, needle and hub are all well known and need not be described. The bottle 4 is formed of a flexible and elastic or resilient plastic such as polyethylene, the wall of the bottle being of such thickness that it retains its normal shape e.g. cylindrical but is readily deformable i.e. collapsed by the pressure of the thumb and finger of the operator and will return to its original shape when the pressure is released. The wall of the bottle should be sufficiently resilient to create a slight suction and thus to draw blood through the needle into the bottle but not so resilient as to prevent collapsing 3,322,114 Patented May 30, 1967 "ice by the pressure of the fingers or to create sutficient suction on the vein from which blood is being drawn to cause collapse of the vein. We have found that a bottle the wall of which is sufficiently rigid to maintain its shape and requires only gentle pressure of the thumb and finger to collapse it will, due to its resilient tendency to return to its original shape when the pressure is relaxed, create sufiicient suction to draw blood from the vein of a human or animal but not sufiicient ordinarily to collapse the vein.
Anticoagulants and phytoagglutinins or lectins are well known and the kinds and quantities thereof required to prevent clotting (anticoagulant) and to cause agglomeration of the blood cells (lectin) of the kind and quantity of the blood sample are well known and need not be discussed. For a disclosure of lectins and their uses We refer to the book entitled Introduction to Immunochemical Specificity by William C. Boyd, John Wiley & Sons, 1962.
The anticoagulant and lectin may be introduced into the bottle 4 either separately or mixed with each other, in either liquid or solid form, and either through the neck of the bottle or through the bottom end of the bottle before it is closed and sealed.
The dispensing nozzle 7 may be of the same shape'as the hub 3 of the needle, and as appears in FIG. 2, contains a filter 8 and has a short neck 9 which serves to deliver a drop of plasma of a suitable size for the spot .test described in Patent No. 3,074,853. The filter 8 may be formed of a variety of filter materials such as pressed glass fiber, pressed nitrocellulose fiber, non-absorbent cotton, filter paper or the like and may be formed by packing loose fiber into the conical end of the nozzle or by cutting a disc from a filter in sheet form and inserting it in the conical space. The filter should be such as will remove solid particles such as blood cells from the plasma but not so difiicultly pervious that the pressure required to force the plasma through the filter will effect hemolysis of the blood cells. It is within the scope of our invention to impregnate the filter with lectin 0r anticoagulant or both to render it more effective i.e. to assist in maintaining the plasma uncoagulated and in agglomerating the blood cells and preventing their passage through the filter. It is also within the scope of our invention to omit the filter.
The apparatus is used as follows.
The bottle 4 containing the lectin and anticoagulant is partially collapsed by the pressure of the thumb and finger and the needle 2 which is attached by its hub 3 to the neck 6 of the bottle is inserted into a vein of a person or animal and the pressure on the bottle released whereupon blood flows through the needle into the bottle and the bottle returns to its normal uncompressed shape. The needle is then withdrawn from the vein and the contents of the bottle shaken to effect mixing of the blood with the lectin and anticoagulant and then allowed to stand whereupon.
the agglomerated blood cells separate and settle to the bottom of the bottle leaving a supernatant layer of clear plasma. By careful manipulation some of the clear plasma generally can be discharged through the needle or the needle may be removed and the plasma discharged through the neck 6. However it is preferred to replace the needle by the dispensing nozzle 7 and to discharge the plasma from the bottle through the filter 8. If preferred the neck of the plastic bottle can be cut-off thereby forming a small test tube from which clear plasma can be removed with a capillary tube.
The whole apparatus is inexpensive and disposable and is not intended for reuse.
1. A device for drawing a sample of blood and separating plasma therefrom comprising a bottle having a transparent or translucent, flexible and resilient side wall capable of being collapsed by the pressure exerted by the thumb and finger of the operator and of returning to its normal shape when said pressure is released, a hypodermic needle attached to said bottle for aspirating blood from a vein of a person or animal into said bottle and an anti coagulant and a lectin in said bottle of a kind and in quantity suflicient to prevent clotting and to cause agglomeration of the cells of the kind and quantity of blood to be delivered into said bottle and to cause the separation of plasma from the collected blood.
2. A device for drawing a sample of blood and sepjarating plasma therefrom as defined in claim 1 in which said dispensing nozzle contains a filter.-
References Cited UNITED STATES PATENTS 2,722,257 11/1955 Lockhart 5. 128-216 X 2,982,286 5/1961 Welch 128216 3,045,494 7/ 1962 Gerarde 128216 X 3,089,489 5/1963 Dunmire 128-216 3,181,529 5/1965 Wilburn 1282 3,192,925 7/1965 Cunningham 128216 3,224,434 12/ 1965 Molomut 1282 RTCHARD A. GAUDET, Primary Examiner. V
S. BROD'ER, Assistant Examiner.
|Cited Patent||Filing date||Publication date||Applicant||Title|
|US2722257 *||Feb 12, 1953||Nov 1, 1955||Compule Corp||Sampling tube equipment|
|US2982286 *||Aug 3, 1956||May 2, 1961||Baxter Laboratories Inc||Blood collection apparatus|
|US3045494 *||Mar 13, 1958||Jul 24, 1962||Gerarde Horace William||Method of providing for blood count and pipette and assembly for use therein|
|US3089489 *||Apr 27, 1959||May 14, 1963||Dunmire Hannah||Aspirating type hypodermic syringes|
|US3181529 *||Jul 30, 1962||May 4, 1965||Wilburn Edgar H||Valved body-fluid sampling tubes|
|US3192925 *||Aug 25, 1961||Jul 6, 1965||Cunningham James Robert||Disposable syringe device|
|US3224434 *||Nov 6, 1962||Dec 21, 1965||Waldemar Medical Res Foundatio||Cell collector|
|Citing Patent||Filing date||Publication date||Applicant||Title|
|US3424148 *||May 10, 1965||Jan 28, 1969||Jacques Blomet||Device for taking blood samples|
|US3460529 *||Jun 30, 1965||Aug 12, 1969||Leucci Gino||Sterile device for extracting urine samples and the like and package for same|
|US3481477 *||Mar 2, 1965||Dec 2, 1969||Andrew F Farr||Apparatus for filtering out clear liquid from suspended solids|
|US3513829 *||Aug 11, 1967||May 26, 1970||Brunswick Corp||Fluid collecting structure|
|US3518804 *||Nov 1, 1966||Jul 7, 1970||Horace W Gerarde||Pipette assembly having precise quantity stabilized reagent in liquid form and method of preparing same|
|US3539300 *||Oct 23, 1967||Nov 10, 1970||Schering Corp||Body fluid collector and separator having improved flow rate|
|US3596652 *||Oct 14, 1968||Aug 3, 1971||Bio Science Labor||Fluid separatory device|
|US3640267 *||Dec 15, 1969||Feb 8, 1972||Damon Corp||Clinical sample container|
|US3718133 *||Jan 12, 1971||Feb 27, 1973||Damon Corp||Container unit for liquid samples|
|US3800780 *||Feb 23, 1972||Apr 2, 1974||Angelika Elliott||Vacuum indicator|
|US3809068 *||Feb 16, 1973||May 7, 1974||Damon Corp||Container unit for liquid sample|
|US3817239 *||May 12, 1972||Jun 18, 1974||Kuntz D||Urine monitor|
|US3951798 *||Feb 25, 1974||Apr 20, 1976||Glasrock Products, Inc.||Container filter combination|
|US3978846 *||Jan 2, 1975||Sep 7, 1976||Bailey Donald L||Syringe for taking blood samples|
|US4014653 *||Dec 26, 1974||Mar 29, 1977||Denver Chemical Manufacturing Company||Micro-filter|
|US4014797 *||May 20, 1975||Mar 29, 1977||Burron Medical Products, Inc.||Intravenous injection apparatus and needle adapter with filter and method of making same|
|US4172448 *||Jul 25, 1977||Oct 30, 1979||Sherwood Medical Industries Inc.||Fluid sampling device|
|US4180060 *||Aug 8, 1977||Dec 25, 1979||Boehringer Mannheim Gmbh||Device for staining biological materials|
|US4227527 *||Oct 23, 1978||Oct 14, 1980||Baxter Travenol Laboratories, Inc.||Sterile air vent|
|US4259187 *||Jun 7, 1979||Mar 31, 1981||Baxter Travenol Laboratories, Inc.||Intravenous fluid filter|
|US4266558 *||Apr 2, 1979||May 12, 1981||American Hospital Supply Corporation||Method of collecting and dispensing a blood sample|
|US4266559 *||Apr 2, 1979||May 12, 1981||American Hospital Supply Corporation||Blood sampler|
|US4298011 *||Sep 7, 1979||Nov 3, 1981||Mangurten Henry H||Blood sample collector|
|US4511351 *||May 14, 1984||Apr 16, 1985||Alza Corporation||Parenteral delivery system utilizing a hollow fiber cellular unit|
|US4511352 *||May 14, 1984||Apr 16, 1985||Alza Corporation||Parenteral delivery system with in-line container|
|US4552556 *||Jan 4, 1985||Nov 12, 1985||Alza Corporation||Parenteral controlled therapy|
|US4579553 *||Jan 7, 1985||Apr 1, 1986||Alza Corporation||Parenteral controlled therapy|
|US4583981 *||Jan 7, 1985||Apr 22, 1986||Alza Corporation||Parenteral controlled therapy, using a porous matrix with parenteral agent|
|US4586922 *||Feb 15, 1985||May 6, 1986||Alza Corporation||Intravenous system for delivering a beneficial agent|
|US4596555 *||Jan 28, 1985||Jun 24, 1986||Alza Corporation||Parenteral delivery system utilizing a hollow fiber cellular unit|
|US4740103 *||Feb 15, 1985||Apr 26, 1988||Alza Corporation||Intravenous system for delivering a beneficial agent|
|US4740197 *||Feb 14, 1985||Apr 26, 1988||Alza Corporation||Intravenous system for delivering a beneficial agent via polymer delivery|
|US4740198 *||Feb 15, 1985||Apr 26, 1988||Alza Corporation||Method of administering intravenous drug using rate-controlled dosage form|
|US4740199 *||Feb 14, 1985||Apr 26, 1988||Alza Corporation||Intravenous system for delivering a beneficial agent|
|US4741734 *||Feb 15, 1985||May 3, 1988||Alza Corporation||Releasing means for adding agent using releasing means to IV fluid|
|US4871360 *||Apr 21, 1986||Oct 3, 1989||Alza Corporation||System for intravenous delivery of a beneficial drug at a regulated rates|
|US4980226 *||Jul 10, 1985||Dec 25, 1990||Molnlycke Ab||Fibrous absorption body for use in disposable articles|
|US5975313 *||Feb 2, 1998||Nov 2, 1999||Sarstewdt Ag & Co.||Blood-tube cap with coagulant additive|
|CN104107053A *||Apr 15, 2014||Oct 22, 2014||贝克顿·迪金森公司||Biological fluid transfer device, biological fluid sampling system and biological fluid separation and testing system|
|EP0856280A2 *||Jan 24, 1998||Aug 5, 1998||Sarstedt AG & Co.||Device for taking blood|
|WO1983000281A1 *||Jul 22, 1982||Feb 3, 1983||American Hospital Supply Corp||Ventable sample collection device|
|WO2014172234A1 *||Apr 14, 2014||Oct 23, 2014||Becton, Dickinson And Company||Biological fluid collection device and biological fluid separation and testing system|
|WO2014172241A1 *||Apr 14, 2014||Oct 23, 2014||Becton, Dickinson And Company||Biological fluid sampling transfer device and biological fluid separation and testing system|
|U.S. Classification||600/578, D24/117, 600/580, 604/212, D24/224, 422/547|
|Cooperative Classification||A61B5/150389, A61B5/153, B01L2200/026, B01L2300/0681, A61B5/150519, A61B5/150717, A61B5/150587, A61B5/150755, B01L2300/0832, B01L3/502, A61B5/1405, B01L2400/0481, A61J1/05, A61B5/15003, A61B5/150099, A61M5/2425|
|European Classification||A61B5/153, A61B5/15B18D6B, A61B5/15B18B2, A61B5/15B4B10, A61B5/15B2D, A61B5/15B18D12F, A61B5/15B26, A61B5/15B18B10D, B01L3/502, A61B5/14B|