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Publication numberUS3572651 A
Publication typeGrant
Publication dateMar 30, 1971
Filing dateApr 28, 1969
Priority dateApr 28, 1969
Publication numberUS 3572651 A, US 3572651A, US-A-3572651, US3572651 A, US3572651A
InventorsWilliam S Harker
Original AssigneeWheaton Industries
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Spin-culture flask for cell culture
US 3572651 A
Abstract  available in
Images(1)
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Claims  available in
Description  (OCR text may contain errors)

United States Patent Inventor Appl. No.

Filed Patented Assignee SPIN-CULTURE FLASK FOR CELL CULTURE 7 Claims, 4 Drawing Figs.

U.S. Cl Int. Cl Field of Search 259/107, 108, (Magnetic Stirrer), 102, 122

Primary Examiner-Robert W. Jenkins Attorney-Schellin and Hoffman ABSTRACT: A flask for the suspension culture of cells in a medium of nutrient substances is provided in which the suspension is given an orbital motion by a magnetic impeller which is suspended in the culture medium. All of the elements in contact with the suspension are inert thereto.

I 20 I I8 2| f' 3---- 7 24 26 27 I i zs IUI- 2.9

SPIN-CULTURE FLASK FOR CELL CULTURE The present invention relates to spin-culture flasks for cell culture and if desired for chemical mixing and the like. According to the present invention, a flask is provided with a magnetic impeller which is rotatably suspended in a suspension of cells in a culture medium to impart an orbital motion thereto. The impeller does not touch the flask either at the bottom thereof or at the sides and thus does not subject any of the suspension to a grinding action as would occur when the impeller is supported in a bearing at the bottom of the flask. Also the impeller does not hurl the cells against the sidewalls of the flask which would damage the cells. Furthermore, all elements are rendered inert to the suspension. 1

in addition, by suspending the impeller in the flask according to the present invention, one eliminates the need for any lubrication which might contaminate the culture medium and one is able to assemble and disassemble theflask easily and quickly. Also, the spin-culture flask is designed for troublefree continuous operation since there are no linkages, gears or other connections.

The present invention will now be described with reference to the accompanying drawings, in which like reference characters refer to like parts and in which:

P16. 1 is a perspective view of the flask according to the present invention;

FIG. 2 is a vertical cross of the flask taken on line 22 of FlG. 3 is a cross-sectional view of the flask taken on line 33 of HG. 2; and,

FIG. 4 is a perspective view of a modification of the magnetic impeller shown in FIG. 3. r

According to FIG. 1, a flask having a closure top 11 and a pouring spout 12 is shown mountedon a base member 13 which is a conventional magnetic-stirring apparatus having a rheostat 14 for controlling the speed. of rotation. As shown, the closure top has fastening means 15 to be described more fully hereinafter.

ln HO. 2, the assembled flask 10 and closure 11 is shown in place on the magnetic stirring apparatus 13. The flask 10 is made of any material which is inert to the culture medium or suspension such as borosilicate glass. As shown, the flask 10 is provided with a pouring spout 12 approximately half way between the bottom and the top of the flask 10. The spout 12 may be provided with any suitable closure element (not shown) such as a glass, cork or rubber stopper, or when desired, a cotton plug or a Morton culture tube closure as shown in US. Letters Pat. No. 2,287,746 may be used. The closure 11 may comprise a body 16 of any suitable rigid material, either metallic or plastic, having a depending skirt or annular flange 17 for securing the closure 11to the flask 10, as by spiral grooves 18 engaging threads 19 at the top of the flask M). The closure 11 may be provided with an inert nonmagnetic liner 20, if desired, for example one of polytetrafluoroethylene, commonly known as Teflon" sold by the E. l. du Pont de Nemours and Company, lnc., of I Wilmington, Del.

At the center of the closure 11 are fastening means 15 which may be nuts and bolts as shown or other means for securing bearing block 21 to the inside of the closure 11 with the liner 20 sandwiched therebetween. The fastening means may be, for example, nylon or teflon machine screws or bolts with associated nuts or may be screws engaging the bearing 1 block 21 or the closure 11. The bearing-block 21 is provided with a recess 22 in the center of the top surface thereof. A bore 23 extends from the recess 22 to the bottom of the block 21 for receiving an impeller shaft 24 provided with a collar 25 at the top thereof. The collar 25 which may be made of stainless steel is supported by the block 21 at the the bottom of recess 22 and in turn supports the shaft 24 which may be also of stainless steel and provided with a teflon coating or it may be disposed within a teflon tube 26. lf desired, the shaft could be made entirely of teflon or could be glass instead of stainless steel, and disposed if desired within a teflon tube.

As also shown in FIG. 3 at the bottom of the shaft 24, there is provided a holder element 27 which may be of teflon or other inert material and is secured to the shaft 24 by screw threads, press fitting or heat sealing in a known manner. A magnetized impeller 28 is provided in the holder 27 and is encapsulated with teflon 29 or other inert material. If desired, the magnetized impeller 28 may be encapsulated in a circular disc-shaped element 30 as shown in FIG. 4 when it is desired to impart the orbital motion to the suspension in this manner rather than by the encapsulated form shown in FIG. 3. ln accordance with the invention, the holder 27 does not touch the bottom of the flask 10 so that the bottom does not act as a support or bearing for the shaft 24 as in prior spin-culture flasks and therefore cannot exert any grinding action which might damage or destroy the cells being subjected to growth culture.

As shown is FIG. 2, the entire flask unit is positioned on a conventional magnetic stirring apparatus 13 which includes a bar magnet 31 mounted on a shaft 32 and driven at controlled speeds by a motor indicated generally at 33. The speed of the motor 33 may be conveniently controlled by the rheostat 14 shown in FIG. 1. The ends of the magnet 31 are equidistant from the bar magnet 28 as shown. When the magnet 31 is rotated, it will cause rotation of magnetized impeller 28 at the same speed.

It will be seen from the foregoing, that the maximum protection to the cells in the suspension is provided since there can be no grinding action as is caused by bearing surfaces, and by controlling the speed of rotation of the impeller 28 the orbital motion of the suspension will keep the cells in suspension in the culture medium and will notcause them to be hurled against the wall of the flask and damage them in this manner.

It will be clear that flasks of various sizes may be employed and that magnetic impellers of different sizes may also be used therewith. Also, it is possible to employ shafts for the impeller so that it is spaced somewhat further from the bottom of the flask than is shownin FIG. 2. s

It will also be understood that while specific materials for the flask and impeller have been described above, any material for any element may be used if it has the necessary structural strength and'is inert to the suspensions being treated.

Although the invention has been described with reference to the specific embodiments shown in the drawings, it will be understoodthat various modifications which would occur to one skilled in the art and which fall within the spirit and scope of the appended claims are intended.

lclaim:

1. A flask for cell culture and the like comprising a nonmagnetic hollow flask with an opening at the top thereof, a closure member surmounting said flask and provided with means for gastight sealing relationship with said opening to seal said opening, a bearing block mounted on the underside of said closure member, said bearing block having a central recess in the upper surface thereof, a bore in said bearing block extending from the bottom thereof to said recess, an elongated stirring member, having a shaft rotatably mounted in said bore, a collar attached to the upper portion of said shaft and rotatably seated in said recess whereby said collar and shaft may rotate in said recess and bore respectively, said shaft being supported by said bearing-block by means of said collar, said shaft extending downwardly from said block and ten'ninating above the bottom of said flask, a magnetized impeller mounted on the lower end of said shaft at a spaced distance above the bottom of said flask and adapted to be rotated about the axis of said shaft.

2. A flask as claimed in claim 1 wherein the bearing block is mounted centrally and axially on the underside of said closure member.

3. The flask of claim 1 wherein an inert polymeric liner is disposed on the underside of said closure member, said liner being sandwiched between said closure member and said bearing block.

4. The flask of claiml wherein the portion of said shaft below the bore in said bearing block is covered with an inert polymeric material;

5. The flask of claim 1 wherein a polymeric holder is atencapsulated withapolytetrafluoroethylene. tached to the lower end of said shaft at a spaced distance Th flask as Claimed in Claim 1 wherein th flask is proabove the bottom of the flask, said holder having a horizontal Vided with a Pouhhg SPout in the of the body intermediate radial bore for receiving said magnetized impeller. the p and honor" thereof- 6. The flask of claim 5 wherein the magnetized impeller is

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US2932493 *Sep 9, 1957Apr 12, 1960Magic Whirl Dispensers IncBeverage mixer
US2951689 *Mar 24, 1958Sep 6, 1960Halogen Insulator And Seal CorMagnetic stirring bar
US2958517 *Apr 28, 1958Nov 1, 1960Bellco Glass IncVessel for tissue culture and the like comprising a magnetic stirrer
US3245665 *Mar 18, 1964Apr 12, 1966Arthur H Thomas CompanyMagnetic mixing bar
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US3975239 *Aug 27, 1973Aug 17, 1976Hans StamerMethod of and incubator for preparing bacterial cultures
US4178209 *Mar 6, 1978Dec 11, 1979Monsanto CompanyContinuous cell culture method and apparatus
US4184916 *Jun 26, 1978Jan 22, 1980Monsanto CompanyContinuous cell culture system
US4225248 *Jul 19, 1978Sep 30, 1980Para Serenella FDevice for mixing and metering the contents of containers, particularly for paints, dyes and the like, and shelf or shelving adopting such a device
US4289854 *Jun 20, 1980Sep 15, 1981Monsanto CompanyCell culture method and apparatus
US4355906 *Apr 3, 1981Oct 26, 1982Bellco Glass Inc.Stirring apparatus for cell culture
US4382685 *Jun 25, 1981May 10, 1983Techne (Cambridge) LimitedMethod and apparatus for stirring particles in suspension such as microcarriers for anchorage-dependent living cells in a liquid culture medium
US4390283 *Jul 6, 1981Jun 28, 1983Beckman Instruments, Inc.Magnetic strirrer for sample container
US4465377 *Jun 7, 1983Aug 14, 1984Techne CorporationMagnetic stirrer apparatus with guided, floating stirrer
US4483623 *Apr 15, 1983Nov 20, 1984Corning Glass WorksMagnetic stirring apparatus
US4512666 *Feb 24, 1984Apr 23, 1985Corning Glass WorksAdjustable height magnetic stirrer
US4534656 *Jun 7, 1983Aug 13, 1985Techne CorporationFloating magnetic stirrer with driving guide rod
US5167449 *Dec 12, 1991Dec 1, 1992Corning IncorporatedPaddle shaft assembly with adjustable-pitch paddles
US5267791 *Dec 13, 1991Dec 7, 1993Corning IncorporatedSuspended cell culture stirring vessel closure and apparatus
US5364184 *Feb 23, 1993Nov 15, 1994General Signal CorporationCoupling method for high power magnetic devices
US5378062 *Feb 23, 1993Jan 3, 1995General Signal CorporationTransfer assembly for disassembly device
US5664938 *Feb 23, 1995Sep 9, 1997Yang; Frank Jiann-FuMixing apparatus for microflow gradient pumping
US5906105 *Mar 21, 1997May 25, 1999Ugonli S.P.A.Machine for producing frozen beverages
US6163095 *Dec 9, 1997Dec 19, 2000Imi Cornelius Inc.Drive system for a frozen food product dispenser
US6670171Jul 9, 2001Dec 30, 2003Wheaton Usa, Inc.Disposable vessel
US6844186Aug 6, 2003Jan 18, 2005Wheaton Usa, Inc.Disposable vessel
US7249880 *Oct 14, 2003Jul 31, 2007Advanced Technology Materials, Inc.Flexible mixing bag for mixing solids, liquids and gases
US7288229 *May 8, 2001Oct 30, 2007Symyx Technologies, Inc.Parallel reactor with sensing of internal properties
US7431494Jul 31, 2007Oct 7, 2008Advanced Technology Materials, Inc.Flexible mixing bag for mixing solids, liquids, and gases
US7651660 *Nov 12, 2001Jan 26, 2010Bayer AktiengesellschaftApparatus for irradiating liquids
US7832923Apr 7, 2007Nov 16, 2010Dynamix Agitators Inc.Mounting assembly for plastic bulk container
US8118478Nov 12, 2010Feb 21, 2012Charles Brian MottMounting assembly for plastic bulk container
US8317389May 11, 2007Nov 27, 2012Ika-Werke Gmbh & Co. KgMicrotiter plate with stirring elements
US8366311 *Apr 21, 2007Feb 5, 2013Atmi BvbaSystems and devices for mixing substances and methods of making same
US8783942 *Apr 18, 2012Jul 22, 2014Metenova Holding AbDevice for stirring
US8794821 *Feb 22, 2008Aug 5, 2014Eppendorf, Inc.Torsionally flexible, sealed drive
US20090323466 *Apr 21, 2007Dec 31, 2009Advanced Technology Materials, Inc.Systems and devices for mixing substances and methods of making same
US20100149907 *Feb 22, 2008Jun 17, 2010New Brunswick Scientific Compnay, IncTorsionally flexible, sealed drive
US20120243366 *Apr 18, 2012Sep 27, 2012Metenova Holding AbDevice For Stirring
USRE43418Oct 7, 2010May 29, 2012Atmi BvbaFlexible mixing bag for mixing solids, liquids, and gases
EP0127970A2 *May 16, 1984Dec 12, 1984Techne IncorporatedMagnetic stirrer apparatus with guided, floating stirrer
EP0154083A2 *Nov 19, 1984Sep 11, 1985Corning Glass WorksMagnetic stirrer
EP0965632A1 *Jun 2, 1999Dec 22, 1999Toyota Gosei Co., Ltd.Continuous fermentation system
EP2293710A1 Apr 22, 2009Mar 16, 2011Compagnie Mediterraneenne Des CafesFrothing machine for creating froth from a drink containing milk
WO2003006633A1 *Jul 9, 2002Jan 23, 2003Wheaton Usa IncDisposable vessel
WO2008000312A1 *May 11, 2007Jan 3, 2008Ika Werke Gmbh & Co KgMicrotiter plate with stirring elements
Classifications
U.S. Classification366/185, 435/302.1, 366/274, 366/273
International ClassificationB01F11/04, C12M1/02, B01F13/08, C12M3/02
Cooperative ClassificationB01F11/04, C12M27/02, B01F13/089, B01F13/0827, C12M23/08
European ClassificationB01F13/08D14, C12M23/08, C12M27/02, B01F13/08D, B01F11/04