|Publication number||US3583230 A|
|Publication date||Jun 8, 1971|
|Filing date||Jun 12, 1968|
|Priority date||Jun 12, 1968|
|Publication number||US 3583230 A, US 3583230A, US-A-3583230, US3583230 A, US3583230A|
|Inventors||Patterson James A|
|Original Assignee||Sondell Research Dev Co|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (10), Referenced by (71), Classifications (15)|
|External Links: USPTO, USPTO Assignment, Espacenet|
United States Patent  Inventor James A. Patterson Los Altos, Calif.
 Appl. No. 736,360
 Filed June 12, 1968  Patented June 8, 1971  Assignee Sondell Research Development Co. Palo Alto, Calif.
 SAMPLE INJECTION METHOD AND APPARATUS 6 Claims, 8 Drawing Figs.
 US. Cl 73/422, 23/230, 23/253, 73/23.1, 73/6I.l 210/78, 210/198, 210/361  Int. Cl ..G01n 31/08  Field of Search 73/421,
421.5, 422, 422 SC, 423, 424, 425, 425.2, 425.4, 421 CA, 23, 23.1, 53, 61.1;210/31 C, 198 C, 78, 361, 362; 23/230, 253
 References Cited UNITED STATES PATENTS 3,201,971 8/1965 Villalobos 73/422(GC)X 3,444,722 5/1969 Roof 73/23.1
3,194,400 7/1965 Herndon, 2l0/198(C) 3,215,500 11/1965 Bittnerv 23/230X 3,240,068 3/1966 Horeth et al..... 73/421.5 3,395,093 7/1968 Libcrti 210/198(C)X 3,397,965 8/1968 Berueffy. 23/230 3,415,627 12/1968 Rait 23/230X 3,437,266 4/1969 Patterson 210/361X FOREIGN PATENTS 1,507,455 12/1967 France 73/422(GC) Primary Examiner-Louis R. Prince Assistant ExaminerHarry C. Post, 111 An0rneyTownsend and Townsend SAMPLE INJECTION METHOD AND APPARATUS This invention relates to a new and improved method and apparatus for preparing and injecting samples into a chromatographic system particularly applicable for injecting samples of animal fluids for chromatographic analysis of amino acids.
According to present chromatographic methods, liquid samples are supplied directly to a chromatographic column by manual or automatic injection. Because the sample is introduced directly. the column must be regenerated between samples to remove impurities. In addition, high pressures accompanying the injection of the sample can compress the absorptive material used in the column and thereby distort the resulting chromatogram of the sample. Another problem encountered in practicing present techniques, is that in the sequential steps performed for injecting sequential samples air can be introduced into the operating column further distorting the chromatogram.
The object of the present invention is to provide a method and apparatus wherein a plurality of samples can be automatically and sequentially injected into a chromatographic system for analysis.
According to one aspect of the present invention, a sample injector is provided in which large molecules such as proteins first removed by centrifuging the sample through a membrane filter and into an injector cartridge containing chromatographic filter material. The sample is then preeluted in the carriage so that predetermined zones of the chromatographic spectrum of the sample can be retained in the cartridge or injected into a chromatographic system. A sequence of samples can thus be continuously applied to a chromatographic column without the necessity of column regeneration between samples.
In accordance with another aspect of this invention the samples applied to the chromatography column are changed continuously and automatically with a positive displacement of all air so that air is not introduced into the operating columnv The injector cartridge also prevents a pressure buildup in the sample and buffer which might be transmitted to the chromatographic column. The sample injector is particularly suitable for the chromatographic analysis of animal fluids, and predetermined amino acids or other molecules can be retained in the injector cartridge and only selected amino acids from the sample injected into the chromatographic column. The chromatographic column can thus be used continuously without regeneration and only the injector cartridges need be regenerated. The invention is also intended to provide means for automatically injecting consecutive samples into a chromatographic system.
In order to accomplish these results, the present invention contemplates providing a plurality of injector cartridges each having a channel therethrough containing a chromatographic filter material, an inlet opening at one end of the cartridge communicating with the channel for loading a sample, and a capillary outlet opening at the other end of the cartridge communicating with the channel for retaining a sample therein until it is ejected through the capillary outlet.
According to another aspect of the invention, a turntable is provided having a plurality of holes through the turntable from one side to the other around the periphery thereof adapted to receive and retain the injector cartridges therein, each hole including spring biasing means to bias an injector cartridge inserted therein in the direction of the side of the turntable from which the injector is inserted.
Samples to be analyzed in a chromatographic system are first centrifuged into respective injector cartridges through loading columns and membrane filters to remove protein. A buffer solution is also introduced into the cartridge by cen trifuging and each cartridge is then placed in the turntable. The sample in each injector cartridge is preeluted for a predetermined period and positioned by the turntable beneath an extendable and retractable loader adapted to engage in sealing relationship the opening of the injector cartridge and extend in a direction against the biasing spring in the hole of the turntable in which the cartridge is'seated. The injector cartridge is then pushed by the extending loader into an orifice in a receiving base positioned on the other side of the turntable from the loader for injection of predetermined zones of the chromatographic spectrum of the sample into a chromatographic system.
From the time that the sample is centrifuged into the injector cartridge and the buffer introduced over the sample, the sample is isolated from the air. Before the extendable loader engages an injector cartridge held in the turntable, a droplet of buffer falls on the inlet opening of the cartridge so that air is not introduced upon sealing engagement of the inlet opening by the cxtendablc loader. Back pressure in the orifice of the receiving base from a chromatographic system also backs up the buffer level into the orifice to meet the capillary outlet from the injector cartridge. No air is thereby introduced into the system during the sample injection. The capillary outlet also serves to prevent premature flow-out of the sample and buffer.
The chromatographic filter material provided in the injector cartridge can be a plurality of cross-linked polymer resin beads mechanically supported within a channel through the injector cartridge. The resin beads can be mechanically supported by screens at either end of the cartridge which also serve to prevent pressure buildup on the resin beads.
Other objects, features and advantages of the present invention will become apparent in the following specification and accompanying drawings.
In the drawings:
FIG. 1 is aside cross-sectional view of an injector cartridge embodying the present invention.
FIGS. IA and 1B are plan views of two types of caps provided over the outlet from the sample injector.
FIG. 2 is a plan view from the top of the injector cartridge illustrated in FIG. 1.
FIG. 3 is a side cross-sectional view of an injector cartridge and loading column in sealing engagement within a centrifuge tube.
FIG. 4 is a fragmentary plan view of an automatic sample injector embodying the present invention.
FIGS. 5 and 6 are detailed side views of the extendable and retractable loader for the automatic sample injector showing an injector cartridge in the turntable before and during injection ofa sample respectively.
As broadly set forth above, the present invention provides a method and apparatus for automatically injecting a plurality of samples into a chromatographic apparatus wherein separate samples are inserted into sample injectors containing a chromatographic filter material and these sample injectors sequentially inserted in the stream of the chromatographic apparatus.
In the embodiment of the present invention illustrated in FIG. 1 there is provided a sample injector 10 having an elongated housing ll of plastic or other suitable material with a chamber or elongated channel formed centrally therein. The channel 11 is lined with a tubular support column 12 of glass or suitable plastic material for supporting the chromatographic absorptive filter material 13 such as microspherical resin beads. Microspherical resin beads for use as the filter material can be formed of the cross-linked copolymer such as, for example, a styrene and divinyl benzene copolymer as set forth in more detail in my U.S. Pat. application Ser. No. 530,051, now Pat. No. 3,463,320, entitled Microsphere Filter and Method of Filtration filed on Feb. 25, 1966. The resin beads 13 are mechanically retained within the support column 12 at each end by screens 14 such a l0 micron mesh screening.
At one end of the sample injector cartridges there is provided an inlet opening 15 communicating with the central channel in which the chromatographic filter material is retained. Positioned at the inlet opening of the cartridge over the screen 14 is a membrane filter 16 sandwiched between glass mat filters 17 which support the membrane filter and provide aprefilter for liquid samples entering the cartridge inlet. At the opposite end of the injector cartridge from the inlet 15, the cartridge is provided with a tapered truncated conical projection 18 having a capillary outlet 19 formed therein communicating with the elongated channel 11' in the cartridge containing the chromatographic filter material 13. The capillary outlet 19 serves to retain fluid in the cartridge before injection. A cap 19a of teflon or other suitable material may be provided over the capillary outlet 19 and truncated conical projection 18, having an angled-slit cut through the cap as illustrated in H0. 1 and FIG. 1A to further retain fluid and release fluid from the cartridge only upon application of pressure on the fluid in the cartridge. Cross angled-slits may also be provided in the cap as illustrated in the cap 191; of FIG. 1B. The injector cartridge 10 illustrated in FIG. 2 is circular in cross section and is provided with a flat surface 20 on one side thereof to facilitate use in the automatic sample injector as hereinafter described.
In accordance with one aspect of the present invention a liquid sampleis introduced into the sample injector cartridge 10 by centrifuging the sample through the membrane filter 16 into the column of resin beads or other chromatographic filter material 13. To this end, a loading column 30 is provide as illustrated in FIG. 3. The loading column is formed of an elongated tube of plastic or other suitable material having an inlet 31 for inserting the sample and an annular projection 32 at the opposite end supporting an O-ring seal 33 for engaging the inlet opening 15 of the injector cartridge 10 in sealing relationship. The samples may then be centrifuged through an outlet passageway 34 through the annular projection 33 of the loading column 30 into the injector cartridge 10.
According to the operation by one aspect of this invention, the injector cartridge 10 is first washed with a buffer solution such as a citrate buffer solution of pH 2.2, and 0.2N in sodium ion, by centrifuging the injector cartridge 10 alone in a centrifuge tube 35. The washings at the bottom of the centrifuge test tube 35 are discarded. The liquid sample such as an animal fluid or a serum with adjusted pH is then applied to the loading column 30 which has been inserted into the inlet opening 15 of the injector cartridge 10. The liquid sample is then centrifuged into the cartridge through the membrane filter 16 including the glass mat filters 17 and screen 14. Centrifuging is accomplished in a centrifuge test tube 35 as illus trated in H6. 3. To insure that the liquid sample has been completely transferred to the injector cartridge, a buffer of the same pH as the sample may be introduced into the loading column 30 and washed through the system by centrifuging in the same manner. The screens 14 which mechanically support the resin beads during loading of the sample into the injector cartridge.
Sufficient buffer is applied during sample loading into the injector cartridge so that the liquid level after centrifuging does not fall below the level of the injector cartridge in the centrifuge tube.
The loaded injector cartridge and loading column are then removed from the centrifuge test tube and the loading column separated from the cartridge. After removing the loading column 30, the glass mats 17 and membrane filter 16 are also removed from the top of the injector cartridge leaving the top screen 14 in place. Proteins filtered by the membrane filter are thereby removed eliminating a source of contamination. The injector cartridge 10 is then inserted into the automatic sample injector assembly illustrated in F103. 4 and for injecting the sample into a chromatographic system.
The automatic sample injector assembly includes a drum or turntable 40 having a plurality of holes 41 formed therethrough from one side to the other around the periphery of the turntable. A flat disc 42 is provided centrally on the turntable 40 so that it slightly overlaps the edge of each of the holes 41 formed around the periphery of the turntable 40. Each hole 41 is adapted to receive a sample injector cartridge with the flat edge 20 of the cartridge facing the disc 42 so that it will fit into the hole 41. The cartridge 10 is then rotated so that it is retained in the hole 41 by the disc 42 until rotated with the flat edge 20 again facing the disc 42. Each hole 41 includes a biasing spring 43 coaxially positioned therein and adapted to engage an annular projection around an injector cartridge to spring bias an inserted injector cartridge against the bottom ofdisc 42.
Means are provided for rotating the turntable 40 relative to an extendable and retractable loader 45 positioned at the edge of the turntable. The extendable and retractable loader 45 includes an extendable and retractable annular projection or piston 46 positioned over the turntable and in alignment with the inlet opening 15 of injector cartridges 10 as they are sequentially positioned beneath the loader 45 by rotation of the turntable 40. The piston 46 on loader 45 extends and retracts in a direction perpendicular to the face of the turntable 40 and supports an O-ring seal 47 therearound. Upon extension of the piston 46, the piston enters and engages the inlet opening 15 of a cartridge 10 in sealing relationship and pushes the cartridge downward in a direction against the biasing spring 43. On the lower side of turntable 40, i.e., the side opposite the extendable and retractable piston 46, there is provided a receiving base 50 stationary relative to the rotating turntable 40. The receiving base 50 includes an orifice 51 and O-ring seal 51a adapted to receive the truncated conical extension 18 at the outlet end of an injector cartridge in sealing relationship as illustrated in FIG. 6. The orifice 51 terminates in a passageway 52 connected to a tube 53 which leadsto a chromatographic system in which the sample is to be injected. Upon extension of the piston 46 from the loader 45 the capillary outlet end of the cartridge 10 is inserted into the orifice 51 so that a buffer under pressure in tube 54 may be transferred through the piston 46 and injector cartridge to inject the sample through the tube 53 into a chromatographic system.
After a loaded injector cartridge is placed in the turntable 40, the cartridge may be delivered to the stationaryloader 45 according to a controlled timing sequence. The controlled timing sequence of delivery of cartridges by the turntable 40 may include a predetermined preelution cycle prior to positioning the cartridge into position beneath the loader 45 for injecting the sample into a chromatographic system. By preelution of the liquid sample in the cartridge, amino acids or other molecules may be removed from the sample to a controlled initial levcl in the chromatographic spectrum of the liquid sample. The cartridges are removed from the injection position beneath the loader 45 after a predetermined time interval so that amino acids or other molecules beyond a desired level in the chromatographic are retained in the cartridge. After injection of predetermined zones of the chromatographic spectrum of the sample into a chromatographic system, the cartridges are removed from the injection position by turntable 40 for regeneration of the filter column of resin beads contained in the cartridge in preparation for loading another liquid sample into the cartridge by centrifuging as heretofore described.
Once a liquid sample and buffer have been centrifuged into the injector cartridge, the sample is maintained in buffer solution and isolated from air throughout the subsequent stages of sample delivery. when the sample injector is removed from the centrifuge test tube after centrifuging, flow-out from the cartridges is prevented by the capillary outlet 19 and cap 190. The cartridge is then inserted in one of the holes in the periphery of the turntable 40. Before each cartridge is delivered to the loader 45 and extendable piston 46 for insertion of the end of the cartridge into the orifice 51 of the receiving base 50, back pressure from the chromatographic system through tube 53 causes the level of buffer to rise in the tapered orifice 51 to receive the capillary outlet 19 and tapered end 18 of the cartridge so that air is not introduced into the system. Furthermore, as a cartridge is delivered by the turntable 40 into alignment beneath the piston 46 on the loader 45, a droplet of buffer falls from the piston 46 onto the top of the cartridge in the inlet opening so that when the piston 46 engages the opening of the injector cartridge forcing the tapered end 16 of the cartridge into the orifice 51, an excess of buffer occupies the space in the inlet so that again air is not introduced into the system. The excess buffer is forced out and collected in a waste tube as the piston 46 extends into the cartridge 10. After the sample is injected from a cartridge into a chromatographic system, the turntable rotates and the cartridge may then be processed through a regeneration cycle. The cartridge is then removed by rotating the cartridge until the flat edge 20 faces the disc 42 so that the biasing spring 43 ejects the cartridge for loading another sample by centrifugmg.
Although only certain embodiments of the present invention have been shown and described, other adaptations and modifications would be apparent without departing from the true spirit and scope of the invention.
l. A sample injector assembly for automatically injecting samples into a chromatographic system comprising: a plurality of sample injector cartridges each having a channel thcrethrough containing a chromatographic filter material, an inlet opening at one end of said cartridge communicating with said channel for loading a sample therein, and a capillary outlet opening at the other end of said cartridge communicating with said channel ejecting a sample thcrethrough; a turntable having a plurality of holes thcrethrough from one side to the other around the periphery thereof adapted to receive said injector cartridges from one side, and retain said cartridges therein, each said hole including spring biasing means to bias an injector cartridge inserted therein in the direction of the side of the turntable from which said injector cartridge is inserted; means for rotating said turntable; means stationary relative to said rotating turntable positioned on one side at the periphery thereof comprising an extendable and retractable loader adapted to engage in sealing relationship the inlet opening of an injector cartridge received in a hole in the periphery of the turntable and extend in a direction against the biasing spring means in said hole; a receiving base stationary relative to said rotating turntable positioned on the side of the turntable opposite and in alignment with the extendable and retractable loader, said receiving base having an orifice therein adapted to receive the capillary outlet opening of an injector cartridge in sealing relationship upon engagement ofa cartridge by said loader and extension of said loader against a biasing spring means.
2. A method of preparing a sample and injecting the sample into a chromatographic system comprising: centrifuging the sample into a chromatographic filter material contained in an injector cartridge and filtering the sample during centrifuging of the sample into the injector cartridge; preeluting the Sample in the chromatographic filter material in the injector cartridge; positioning the injector cartridge for injecting the sample into a chromatographic system; and injecting desired zones of the chromatographic spectrum of the sample from the injector cartridge into the chromatographic system.
3. A method of preparing a sample and injecting the sample into a chromatographic system comprising: centrifuging the sample through a membrane filter into a chromatographic filter material contained in an injector cartridge; centrifuging a buffer solution through themembrane filter and into the chromatographic filter contained in the injector cartridge; preeluting the sample in the chromatographic filter material in the injector cartridge; positioning the injector cartridge for injecting the sample into a chromatographic system; injecting predetermined zones of the chromatographic spectrum of the sample from the injector cartridge into the chromatographic system.
4. A sample injector assembly for automatically injecting samples into a chromatographic system comprising: a fluid line arranged for directing fluid and sample material to said chromatographic system; a plurality of sample injector cartridges, each having a chamber therein, chromatographic filter material locate in said chamber, means defining an inlet into said chamber for loading a sample therein, and means defining an outlet from said chamber for ejecting a sample therefrom; table means having a plurality of holes formed thcrethrough to receive the injector cartridges, each said hole including resilient biasing means to bias an injector cartridge inserted therein in a direction away from the table; said fluid line connected at one end to said chromatographic system and at its other end to a position adjacent said table means; means translating said table means relative to the fluid line for successively positioning said holes in alignment therewith; and means engaging an injector cartridge contained within a hole in the table in alignment wit said fluid line to depress the injector cartridge against the resilient biasing means, thereby to engage the outlet from said injector cartridge and the end of the fluid passageway in sealing relationship.
5. A sample injector for a chromatographic system comprising: an elongated injector cartridge having a channel thcrethrough containing a chromatographic filter material, an inlet opening at one end of said cartridge communicating with said channel for loading a sample therein and a capillary outlet opening at the other end of said cartridge communicating with said channel for ejecting a sample thcrethrough, a membrane filter in the inlet opening end of said cartridge, and at least one screen placed in the inlet opening end of said injector cartridge to prevent pressure buildup in the injector cartridge on the chromatographic filter material.
6. A sample injector for a chromatographic system comprising: an elongated injector cartridge having a channel thcrethrough containing a chromatographic filter material, an inlet opening at one end of said cartridge communicating with said channel for loading a sample therein and a capillary outlet opening at the other end of said cartridge communicating with said channel for ejecting a sample thcrethrough, said chromatographic filter material comprising a plurality of microspherical cross-linked polymer resin beads, and a tubular support column coaxially mounted within the channel and fine mesh screening fixed at each end of the support column for containing said resin beads.
|Cited Patent||Filing date||Publication date||Applicant||Title|
|US3194400 *||Dec 21, 1960||Jul 13, 1965||Malvern Inst For Psychiatric A||Centrifugal solvent extraction|
|US3201971 *||Oct 8, 1962||Aug 24, 1965||Beckman Instruments Inc||Gas chromatograph|
|US3215500 *||Jun 12, 1961||Nov 2, 1965||Donald L Bittner||Laboratory mixer-separator|
|US3240068 *||Jul 12, 1963||Mar 15, 1966||Exxon Production Research Co||Mud-gas sampling system|
|US3395093 *||Sep 27, 1967||Jul 30, 1968||Research Corp||Centrifugal chromatography and electrophoresis device|
|US3397965 *||Jun 17, 1965||Aug 20, 1968||Robert R. Berueffy||Gas analysis unit|
|US3415627 *||Mar 29, 1966||Dec 10, 1968||Joseph M. Rait||Chemical testing apparatus|
|US3437266 *||Jul 3, 1967||Apr 8, 1969||Sondell Research & Dev Co||Centrifugal separation enhancement|
|US3444722 *||Sep 9, 1966||May 20, 1969||Phillips Petroleum Co||Device for supplying carrier gas to transport eluted portion of sample and to backflush chromatographic column|
|FR1507455A *||Title not available|
|Citing Patent||Filing date||Publication date||Applicant||Title|
|US3741400 *||Jun 15, 1970||Jun 26, 1973||J Dick||Blood sample container|
|US3810545 *||Dec 29, 1972||May 14, 1974||Du Pont||Centrifuge chromatography apparatus and system|
|US3830369 *||Dec 11, 1973||Aug 20, 1974||Pfadenhauer E||High pressure gradient chamber for liquid chromatography|
|US3879127 *||Jul 12, 1972||Apr 22, 1975||Technicon Instr||Method of sample preparation for sample analysis|
|US3902372 *||Dec 13, 1973||Sep 2, 1975||Durrum Dev Corp||Sample carrying vial and means for introducing the vial and its liquid sample into liquid analyzing system|
|US3922223 *||Mar 25, 1974||Nov 25, 1975||Us Navy||Multiple chromatographic column system|
|US3963614 *||Nov 5, 1971||Jun 15, 1976||Hitachi, Ltd.||Apparatus for removing unnecessary components from samples|
|US3981801 *||Feb 14, 1974||Sep 21, 1976||University Of Edinburgh||Gradient storage method for liquid chromatography|
|US3985166 *||Jun 2, 1975||Oct 12, 1976||Klee Richard E||High-pressure sample injection for liquid chromatography|
|US3999439 *||Jul 22, 1974||Dec 28, 1976||Varian Associates||High pressure sample injector and injection method|
|US4422941 *||Sep 8, 1980||Dec 27, 1983||University Of Pittsburgh||Apparatus for liquid-solid column centrifugation chromatography and method|
|US4874520 *||Jun 23, 1988||Oct 17, 1989||Battelle Development Corporation||Chromatographic process|
|US5079959 *||Sep 8, 1989||Jan 14, 1992||Hitachi, Ltd.||Analyzing system using sheath flow of sample|
|US5358641 *||May 10, 1993||Oct 25, 1994||Helena Laboratories Corporation||Column analyzer system and improved chromatograph column for use in the system|
|US5395521 *||May 31, 1991||Mar 7, 1995||Board Of Regents, The University Of Texas System||Automated column equilibration, column loading, column washing and column elution|
|US5441645 *||Sep 16, 1994||Aug 15, 1995||Helena Laboratories Corporation||Column analyzer system and improved chromatograph column for use in the system|
|US5443734 *||Aug 3, 1993||Aug 22, 1995||Applied Separations, Inc.||Programmable solid phase extraction and elution device|
|US5472598 *||Apr 15, 1994||Dec 5, 1995||Upchurch Scientific, Inc.||Connection assembly for liquid chromatography columns|
|US5482628 *||Apr 15, 1994||Jan 9, 1996||Upchurch Scientific, Inc.||Column for liquid chromatography|
|US5512168 *||Jun 7, 1995||Apr 30, 1996||Applied Separations, Inc.||Programmable solid phase extraction and elution device|
|US5517870 *||Dec 22, 1993||May 21, 1996||Hitachi, Ltd.||Intra-liquid particle classification apparatus using light scattering|
|US5585068 *||Oct 14, 1994||Dec 17, 1996||Biochemical Diagnostics, Inc.||Apparatus for automatically separating a compound from a plurality of discrete liquid specimens|
|US5589063 *||Jun 6, 1995||Dec 31, 1996||Helena Laboratories Corporation||Column analyzer system and improved chromatograph column for use in the system|
|US5595664 *||Jun 6, 1995||Jan 21, 1997||Helena Laboratories Corporation||Column analyzer system and improved chromatograph column for use in the system|
|US5651885 *||Jan 3, 1996||Jul 29, 1997||Schick; Hans G.||Column for liquid chromatography|
|US5736036 *||Jan 31, 1995||Apr 7, 1998||Upchurch Scientific, Inc.||Column for liquid chromatography|
|US5766460 *||Jan 6, 1997||Jun 16, 1998||Pharmacia Biotech Ab||Liquid chromatographic system|
|US5863428 *||Apr 1, 1997||Jan 26, 1999||Phenomenex||Guard cartridge for chromatography|
|US5932174 *||May 30, 1997||Aug 3, 1999||Hach Company||Device for chloride ion removal prior to chemical oxygen demand analysis|
|US6193883 *||Jun 30, 1997||Feb 27, 2001||Gesellscgaft Fur Biotechnologische Forschung Mbh (Grf)||Microfiltration apparatus|
|US6270730 *||Jun 16, 1998||Aug 7, 2001||Northwest Engineering Inc.||Multi-well rotary synthesizer|
|US6296764 *||Nov 4, 1998||Oct 2, 2001||Lamina, Inc.||Apparatus for mixing and separating particulate matter from a fluid|
|US6344032 *||Aug 8, 2000||Feb 5, 2002||Safety Syringes, Inc.||Disposable self-shielding unit dose syringe guard|
|US6811755||Jul 9, 2001||Nov 2, 2004||Mcluen Design, Inc.||Multi-well rotary synthesizer|
|US7150998||Dec 19, 2000||Dec 19, 2006||Mcluen Design, Inc.||Multi-well rotary synthesizer|
|US7192558||Dec 19, 2000||Mar 20, 2007||Mcluen Design, Inc.||Multi-well rotary synthesizer|
|US7435598 *||Nov 10, 2003||Oct 14, 2008||Exxonmobil Chemical Patents Inc.||Catalyst testing apparatus and process|
|US7482164 *||Jul 13, 2007||Jan 27, 2009||Exxonmobil Research And Engineering Company||Rapid serial experimentation of catalysts and catalyst systems|
|US7553448||Jun 19, 2003||Jun 30, 2009||Bioveris Corporation||Electrochemiluminescence flow cell and flow cell components|
|US7608462 *||Apr 26, 2005||Oct 27, 2009||Bruker Biospin Gmbh||Drying SPE cartridges|
|US7682505 *||Feb 14, 2005||Mar 23, 2010||Bio-Rad Pasteur||Method and devices for dry loading of chromatography resins|
|US8147776||May 27, 2008||Apr 3, 2012||Mcluen Design, Inc.||Multi-well rotary synthesizer|
|US8158085||Dec 19, 2000||Apr 17, 2012||Mcluen Design, Inc.||Multi-well rotary synthesizer|
|US8211279||Jun 2, 2006||Jul 3, 2012||Board Of Regents Of The University Of Texas System||Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode|
|US8372044||May 20, 2005||Feb 12, 2013||Safety Syringes, Inc.||Syringe with needle guard injection device|
|US8404196||Feb 28, 2012||Mar 26, 2013||Mcluen Design, Inc.||Multi-well rotary synthesizer|
|US8702958||May 31, 2012||Apr 22, 2014||Board Of Regents Of The University Of Texas System||Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode|
|US8747780||Feb 25, 2013||Jun 10, 2014||Mcluen Design, Inc.||Multi-well rotary synthesizer|
|US8840774||Oct 28, 2013||Sep 23, 2014||Board Of Regents Of The University Of Texas System||Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode|
|US9068954 *||Mar 28, 2012||Jun 30, 2015||Catalytic Combustion Corporation||Monolith catalyst test system and method for its use|
|US9069358||Jun 24, 2013||Jun 30, 2015||Biolytic Lab Performance, Inc.||System for controlling and optimizing reactions in solid phase synthesis of small molecules|
|US9341545||Mar 12, 2014||May 17, 2016||Ceramex Ltd||Testing catalytic efficiency of an exhaust component|
|US9562880||Jun 29, 2015||Feb 7, 2017||Catalytic Combustion Corporation||Monolith catalyst test system and method for its use|
|US20010000723 *||Dec 19, 2000||May 3, 2001||Mcluen Gary R.||Multi-well rotary synthesizer|
|US20010001035 *||Dec 19, 2000||May 10, 2001||Northwest Engineering Inc.||Multi-well rotary synthesizer|
|US20010007644 *||Dec 19, 2000||Jul 12, 2001||Mcluen Gary R.||Multi-well rotary synthesizer|
|US20010051114 *||Jul 9, 2001||Dec 13, 2001||Mcluen Gary R.||Multi-well rotary synthesizer|
|US20030118477 *||Dec 6, 2002||Jun 26, 2003||Igen International, Inc.||Apparatus and methods for carrying out electrochemiluminescence test measurements|
|US20040090168 *||Jun 19, 2003||May 13, 2004||Kumar Sudeep M.||Electrochemiluminescence flow cell and flow cell components|
|US20050054987 *||Oct 21, 2004||Mar 10, 2005||Safety Syringes, Inc.||Disposable self-shielding unit dose syringe guard|
|US20050101022 *||Nov 10, 2003||May 12, 2005||Vaughn Stephen N.||Catalyst testing apparatus and process|
|US20050252859 *||Apr 26, 2005||Nov 17, 2005||Bruker Biospin Gmbh||Drying SPE cartridges|
|US20060246593 *||Jan 3, 2003||Nov 2, 2006||Towler Gavin P||Material processing apparatus and method|
|US20070034529 *||Jun 2, 2006||Feb 15, 2007||Bard Allen J||Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode|
|US20070193933 *||Feb 14, 2005||Aug 23, 2007||Pierre Vidalinc||Method and devices for dry loading of chromatography resins|
|US20080014639 *||Jul 13, 2007||Jan 17, 2008||Vincent Matthew J||Rapid serial experimentation of catalysts and catalyst systems|
|US20110290731 *||May 26, 2011||Dec 1, 2011||Ozbal Can C||Cartridge changers and methods for utilizing the same|
|US20140255277 *||May 21, 2014||Sep 11, 2014||Mcluen Design, Inc.||Multi-well rotary synthesizer|
|EP0425297A2 *||Oct 26, 1990||May 2, 1991||Helena Laboratories Corporation||Column analyzer system|
|EP0425297A3 *||Oct 26, 1990||May 5, 1993||Helena Laboratories Corporation||Column analyzer system|
|WO2003095196A1 *||May 5, 2003||Nov 20, 2003||Techelan, Llc.||Apparatus and method for high throughput analysis of compound-membrane interactions|
|U.S. Classification||73/864.85, 422/64, 210/198.2, 436/177, 210/361, 73/61.56, 73/23.42, 210/781, 422/70|
|International Classification||G01N30/60, G01N30/00, G01N30/24|
|Cooperative Classification||G01N30/24, G01N30/6091|