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Publication numberUS3623611 A
Publication typeGrant
Publication dateNov 30, 1971
Filing dateNov 10, 1969
Priority dateNov 10, 1969
Publication numberUS 3623611 A, US 3623611A, US-A-3623611, US3623611 A, US3623611A
InventorsReitz Richard H, Riley William H
Original AssigneeDow Chemical Co
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Microdialyzer apparatus
US 3623611 A
Abstract  available in
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Claims  available in
Description  (OCR text may contain errors)

United States Patent Inventors William 11. Riley;

Richard H. Reitz, both of Midland, Mich. 875,020

Nov. 10, 1969 Nov. 30, 1971 The Dow Chemical Company Midland. Mich.

Appl. No. Filed Patented Assignee MICRODIALYZER APPARATUS 5 Claims, 3 Drawing Figs.

US. Cl 210/321 lnt.Cl 801d 31/00 Field of Search i. 210/22,

[56] References Cited UNITED STATES PATENTS 3,211,645 10/1965 Ferrari 210/321 X 3,333.706 8/1967 lsreeli..... '210/321 3,341,299 9/1967 Catravas. 23/253 X 3.425.560 2/1969 Smythe 210/321 X Primary E.raminerF rank A. Spear, .l r. Attorneys-Griswold and Burdick and Earl D Ayers MICRODIALYZER APPARATUS BACKGROUND OF THE INVENTION This invention relates to dialysis apparatus and particularly to microdialyzer apparatus,

Although the desirability of using microdialysis techniques has been recognized that, the practice has been limited because of the difi'lculty in manipulating dialysis tubing sacs or the expense of securing sandwich-supported devices which do not leak.

Accordingly, a principal object of this invention is to provide improved microdialyzer apparatus.

Another object of this invention is to provide improved, more economical microdialyzer apparatus.

A further object of this invention is to provide improved, easy to use and easy to assemble and disassemble microdialyzer apparatus.

In accordance with this invention there is provided microdialyzer apparatus including a pair of elastic, fluid-impervious sheets usually having a plurality ofcutout parts which communicate with one edge of the sheets, the cutout parts in each sheet being aligned with each other and separated by a penneable membrane. Rigid backup sheets are held under pressure against each of the elastic sheets to define fluidtight pockets with the cutout parts.

The invention, as well as additional objects and advantages thereof, will best be understood when the following detailed description is read in connection with the accompanying drawing, in which:

FIG. 1 is a plan view of apparatus in accordance with this invention;

FIG. 2 is a side elevational view of an elastic sheet element in accordance with this invention, and

HO. 3 is a sectional view taken along the line 33 of FIG. 1.

Referring to the drawing, there is shown microdialyzer apparatus, indicated generally by the numeral 10, comprising backing plates 12, 14, usually made of glass, elastic sheet elements 16, 18, usually made of so-called medical or food-grade Silastic sheeting; and a permeable membrane 20, a cellulose membrane, for example, sandwiched between the elastic sheets 16, 18.

The assembly is held together, under pressure by clamps 22, 24.

Referring to FIGS. 2 and 3, it may be seen that the elastic sheets 16, 18 each have cutout parts 26a, 26b, respectively, which are aligned with each other when the apparatus is assembled for operative use. Each of the cutout parts 26a, 26b communicates with a lengthwise edge of its elastic sheet (28 in FIG. 2).

1n the apparatus as assembled, a plurality of fluid-receiving pockets are formed with the cutout part of the elastic sheets forming sidewalls and the membrane 20 or backing plate 12 or 14 forming the front wall and backwall, respectively.

A stopper 30, shown in FIG. 3, usually closes the pockets after they are loaded with liquid.

In operation, as is well known in the art, measured volumes of different liquid-based materials are placed in respective pockets separated by the membrane 20, and usually are vibrated to shorten the time required for sufficient dialysis to take place The amount of liquid-based material remaining in each pocket is then carefully withdrawn and measured.

While the rigid plates 12, 14 are usually made of glass, other materials which are rigid, fluid-impervious and nonreactive with the materials involved in the dialysis may be used.

Similarly, the sheets 16, 18 may be made of any suitable elastic material which is fluid-impervious impervious and nonreactive to the materials involved in the dialysis.

The simple sandwich-type construction is easily assembled and disassembled, easily cleaned, and is inexpensive to manufacture.

ln one example of apparatus in accordance with the invention the sheets l6, 18 are made of Silastic medical grade material 0.120 inch in thickness, 1 inch wide and 6 inches long. The cutout parts are made using a No. 4 (for example) cork borer centered three-eighths inch below one edge and spaced apart at least one-half inch. Small channels are cut from the cork borer bored holes to the adjacent edge of the sheet with a razor blade. The elastic sheets are then glued to 1- inch by 6-inch by A-inch plate glass by Silastic medical adhesive, Brand A, and allowed to set 24 hours before using. In some instances gluing of the sheets 16, 18 to the backing plate is not necessary.

Rubberlike silicone materials other than Silastic sheeting may be used to make the sheets 16, 18.

What is claimed is:

l. Microdialyzer apparatus comprising a sheet of permeable membrane, a pair of sheets of liquid-impermeable elastic material, said sheets each having one side abutting against said membrane, each of said sheets having at least one cutout part, each of said cutout parts communicating with an edge of its sheet, said sheets being so aligned that identical cutouts are adjacent to each other on opposite sides of said membrane, a pair of rigid liquid-impermeable backing plate elements, one of said backing plate elements abutting against each of said sheets on the side of the sheet which is remote from said membrane, said plates covering said cutout parts, clamping means, said clamping means being coupled across said backing plates, and means for capping said cutout parts along said edges.

2. Apparatus in accordance with claim 1, wherein said backer plates are made ofglass.

3. Apparatus in accordance with claim 1, wherein said sheets are made of medical or food-grade rubberlike silicone sheeting.

4. Apparatus in accordance with claim 1, wherein each of said sheets has an array of cutout pans, each of said cutout parts being the same size.

5. Apparatus in accordance with claim 1, wherein said sheets are bonded to said backing plates.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US3211645 *Jul 17, 1962Oct 12, 1965Technicon InstrMethod and apparatus for filtering sanguineous liquid streams
US3333706 *Oct 6, 1965Aug 1, 1967Technicon InstrDialyzer assembling means
US3341299 *Aug 14, 1964Sep 12, 1967Technicon CorpChromatography analysis apparatus and method
US3425560 *Oct 2, 1967Feb 4, 1969Technician CorpDialyzer assembling means
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US5183564 *Dec 5, 1991Feb 2, 1993Hong Chin ChenStirring device for facilitating dialysis
US5733442 *Apr 11, 1994Mar 31, 1998Shukla; Ashok K.Microdialysis/Microelectrodialysis system
US6458275 *Jun 5, 2000Oct 1, 2002Harvard Apparatus, Inc.Multi-well equilibrium dialysis system
US6776908 *Sep 28, 2000Aug 17, 2004Pfizer Inc.Micro-equilibrium dialysis vertically-loaded apparatus
US7863015Sep 13, 2005Jan 4, 2011Roche Diagnostics GmbhApparatus for concurrent in vitro protein synthesis of multiple proteins; cell-free translation
WO2001093979A1 *Jun 5, 2001Dec 13, 2001Andrew CreaseyMulti-well equilibrium dialysis systems
U.S. Classification210/321.84
International ClassificationB01D61/24, B01D61/28
Cooperative ClassificationB01D61/28
European ClassificationB01D61/28