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Publication numberUS3773468 A
Publication typeGrant
Publication dateNov 20, 1973
Filing dateApr 3, 1972
Priority dateApr 3, 1972
Publication numberUS 3773468 A, US 3773468A, US-A-3773468, US3773468 A, US3773468A
InventorsR Hubbard, W Matthew
Original AssigneeR Hubbard, W Matthew
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Centrifugable extraction and hohogenization vessel
US 3773468 A
Abstract
A glass tube closed at one end and open at the other, said open end being threaded, said tube being adapted, on the one hand, to receive a pestle slidingly within it, the interior surface of the tube and the exterior surface of the pestle being roughened so as to effect grinding of the material within the tube and, on the other hand, to receive a cap for closing the tube for shaking its content together with a solvent and for sealing the contents within the tube during centrifugation.
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Description  (OCR text may contain errors)

United States Patent [1 1 Hubbard et al.

[ CENTRIFUGABLE EXTRACTION AND HOHOGENIZATION VESSEL [76] Inventors: Roger W. Hubbard, 488 Winter St., Framingham, Mass. 01701; William T. Matthew, 35 Grove St., Hopkinton, Mass. 01748 [22] Filed: Apr. 3, 1972 [21] App]. No.2 240,373

[52] US. Cl. 23/259, 23/292 [58] Field of Search 23/259, 292, 253; 73/425, 425.2

[56] References Cited UNITED STATES PATENTS 3,072,362 1/1963 Allen 243/35 3,459,369 8/1969 Marks 23/292 X Nov. 20, 1973 3,464,798 9/1969 Kilthau 23/292 X 3,565,582 2/1971 Young 23/253 X 3,684,455 8/1972 Vacirca et al. 23/292 X Primary Examiner-Morris O. Wolk Assistant Examiner-R. E. Serwin Attorney-Robert T. Gammons et a1.

[57] ABSTRACT A glass tube closed at one end and open at the other, said open end being threaded, said tube being adapted, on the one hand, to receive a pestle slidingly within it, the interior surface of the tube and the exterior surface of the pestle being roughened so as to effect grinding of the material within the tube and, on the other hand, to receive a cap for closing the tube for shaking its content together with a solvent and for sealing the contents within the tube during centrifugation.

8 Claims, 7 Drawing Figures .CENTRIFUGABLE EXTRACTION AND I-IOI-IOGENIZATION VESSEL BACKGROUND OF THE INVENTION The usualpractice-in taking samples and extractions is to subject :the'material to be sampled to comminution witha motarand'pes'tle, transfer it to a test tube where it is shakenxup-with a solvent and then retransferring it to a centrifuge device for centrifugation. When very smallamounts of 'material are all that is available for testing the several transfers result in substantial loss of material consequently making the tests somewhat unreliable. There is no apparatus presently available in which a very small amount of material can be ground up,-extracted and centrifuged in a single piece of equipment. It is accordingly the purpose of this invention to provide anapparatus in which all three operations may be performed without transferring the material from one receptacle to. another so that even the smaLlest sample can be processed successfully.

SUMMARY As herein illustrated, the apparatus comprises a hollow tubeclosed at one end and open at the other, said tube having a uniform interior cross-section throughout the major portion of its length from end to end, the surface of which is'roughened, said tube being designed alternately to receive a pestle provided with a grinding head having a cross-section corresponding to that of the interior of the tube adapted to be slidably moved in the tube by means of a handle attached thereto to effect grinding of the material contained in the tube and to receive a cap for sealing the open top for agitation of the ground material in a solvent and/or sealing the tube during the subsequent centrifugation. The tube and pestle are comprised of glass, the interior surface of the tube and the exterior surface of the pestle being roughened and being slidably fitted with a clearance in the order of 0.004 inches. The tube has a wall thickness in the order of 0.094 inches, its open end is threaded and the cap is comprised of plastic containing threads by means of which it is threaded onto the open end of the tube. The top of the cap at the inside is fitted with a Teflon disc.

The invention will now be described in greater detail with reference to the accompanying drawings wherein:

FIG. 1 diagrammatically illustrates a centrifuge for receiving a capped tube for 'centrifugation;

FIG. 2 is a diametrical section of one component of the sampling apparatus comprising a hollow tube closed at one end and open at the other;

FIG. 3 is an elevation of another component of the sampling apparatus comprising a pestle adapted to be slidably engaged within the tube;

FIG. 4 is a diametrical section of a modified form of the tube shown in FIG. 2;

FIG. 5 is an elevation of a pestle adapted to be used with the tube shown in FIG.'4;

FIG. 6 is an enlarged diametrical section of another component of the sampling apparatus comprising a cap adapted to be mounted to the open end of the tube; and

FIG. 7 is a section of a sealing disc.

Referring to the drawings, the sampling apparatus comprisesessentially three components, to wit, a tube 10, a pestle 1'2 and'a cap 14.

The tube 10 has a closed end 16 and an open end 18. The interior of the tube is of uniform cross-section The pestle 12 has a grinding head 26, the axial length of which is approximately 1.25 inches, the crosssection of which corresponds to that of the interior cross-section of the tube and which has an outside diameter of approximately 0.508 inches. The head is provided with a handle 28 which measures from one end of the head to its distal end approximately 7 inches.

The pestle is adapted to be slidably interengaged with the interior of the tube and to be manipulated therein by means of a handle 28 to grind material placed in the tube and to this end the entire interior surface of the tube is etched or mechanically roughened and the exterior surface of the head of the pestle is correspondingly etched or roughened.

A plastic cap 14 is provided for sealing the content of the tube within the tube during shaking with a solvent and for the centrifugation. The cap 14 contains threads 32 by means of which it is threaded to the open end of the tube and has within it at the top a Teflon disc 34 which forms a seal between the cap and the open end of the tube. The Teflon disc is approximately 0.079 inches thick.

FIGS. 4 and 5 show an alternative form of the apparatus wherein the closed end of the tube is conical and the end of the head of the pestle is correspondingly shaped.

FIG. 1 shows diagrammatically a centrifuge comprising a rotor 36 rotatable about a vertical axis X-X, the rotor containing pockets 38 for receiving capped tubes. Desirably to protect the tubes and to hold them snugly engaged within the pockets, tubular rubber sleeves 39 are inserted into the pockets to cushion the capped tubes.

The procedure with such a piece of apparatus is to place the sample to be examined or tested in the tube, effect its comminution by reciprocating the pestle therein, remove the pestle, add a solvent, apply the cap and shake the material to dissolve it in the solvent and then mount the capped tube or tubes in the centrifuge shown in FIG. 1 to effect centrifugation.

It should be understood that the present disclosure is for the purpose of illustration only and that this invention includes all modifications and equivalents falling within the scope of the appended claims.

We claim:

1. A sampling assembly comprising a hollow tube closed at one end and open at the other, said tube having a uniform interior cross-section throughout the major portion of its length from end to end, the surface of which is roughened, a cylindrical grinding head having a cross-section corresponding to that of the interior of the tube, the surface of which is roughened like that of the interior of the tube, a handle fixed at one end to one end of the head by means of which the head may be moved in shearing engagement with the interior of the tube to grind material contained in the tube, a cap for closing the open end of the tube following grinding, and a thread circumferentially of the open end of the tube onto which the cap is adapted to be screwed for sealing the tube incident to centrifugation of the content of the tube.

2. Apparatus according to claim 1, wherein the difference between the inside diameter of the tube and the outside diameter of the head of the pestle is in the order of 0.004 inches.

3. Apparatus according to claim 1, wherein the pestle has a roughened surface which extends axially thereof and is in the order of 1.25 inches.

4. Apparatus according to claim 1, wherein the interior wall of the tube at the closed end is semispherical and the end of the head of the pestle is correspondingly the cap and the open end of the tube.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US3072362 *May 17, 1962Jan 8, 1963Elvy W AllenVial holder
US3459369 *Sep 16, 1965Aug 5, 1969Beckman Instruments IncCentrifuge test tube cap
US3464798 *Nov 16, 1965Sep 2, 1969Gustave Frederick KilthauIon-exchange or chemical interaction plastic form
US3565582 *Nov 30, 1967Feb 23, 1971Robert R YoungMethods and means for handling blood test specimens
US3684455 *Dec 19, 1969Aug 15, 1972Mallinckrodt Chemical WorksApparatus for mixing liquids
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US3865551 *Nov 17, 1972Feb 11, 1975Atsushi SaikiLaboratory analysis instrument
US3933440 *Aug 12, 1974Jan 20, 1976International Telephone And Telegraph CorporationChemical reaction vessel
US4013419 *Jan 30, 1976Mar 22, 1977Betzer Peter RDigestion bomb
US4074824 *Dec 3, 1975Feb 21, 1978Kontes Glass CompanyContainer for storage and shipment of chemical standards, radioactive isotopes and the like
US4366930 *Apr 27, 1981Jan 4, 1983Trombetti Jr Albert VTablet pulverizer
US4397425 *May 19, 1980Aug 9, 1983Pennzoil CompanyGrinding apparatus
US5174508 *Aug 16, 1991Dec 29, 1992Source For Automation, Inc.Knife-edge impellers to pulverize, circulate solvent; automated; drug analysis
US5244635 *Jun 19, 1992Sep 14, 1993Cirrus Diagnostics, Inc.Centrifuge vessel with coaxial waste chamber having cap to prevent waste fluid transfer from the chamber into the vessel
US5533683 *Dec 20, 1994Jul 9, 1996Biomedical Polymers, Inc.Tissue grinding system
US5593097 *Jun 10, 1994Jan 14, 1997Eastman Kodak CompanyMicro media mill and method of its use
US5829696 *Aug 27, 1997Nov 3, 1998Michelle S. DeStefanoApparatus for grinding a specimen such as a tissue sample
US5871700 *Nov 21, 1997Feb 16, 1999C.A. Greiner & Sohne Gesellschaft M.B.H.Holders with seals
EP0014179A1 *Jan 14, 1980Aug 6, 1980Ciba-Geigy AgDevice for extracting components from solid and semi-solid materials
WO1994000762A1 *Jun 8, 1993Jan 6, 1994Cirrus Diagnostics IncCentrifuge vessel for automated solid-phase immunoassay
Classifications
U.S. Classification422/550, 215/379, 241/199.11, 215/44, 422/918, 241/169.2
International ClassificationB01L3/14
Cooperative ClassificationB01L3/5021
European ClassificationB01L3/5021