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Publication numberUS3829223 A
Publication typeGrant
Publication dateAug 13, 1974
Filing dateJul 20, 1973
Priority dateJul 20, 1973
Publication numberUS 3829223 A, US 3829223A, US-A-3829223, US3829223 A, US3829223A
InventorsS Hamel
Original AssigneeAtomic Energy Commission
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Mixing rotor for fast analyzer of rotary cuvette type with means for enhancing the mixing of sample and reagent liquids
US 3829223 A
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Description  (OCR text may contain errors)

United States Patent [191 Hamel Aug. 13, 1974 MIXING ROTOR FOR FAST ANALYZER OF ROTARY CUVETTE TYPE WITH MEANS FOR ENHANCING THE MIXING OF SAMPLE AND REAGENT LIQUIDS [75] lnventor: Stephen D. Hamel, Oak Ridge,

Tenn.

[73] Assignee: The United States of America as represented by the Secretary of the United States Atomic Energy Commission, Washington, DC.

22 Filed: July 20, 1973 21 Appl. No.: 381,297

52 US. Cl ..356/246, 23/259, 250/576, 356/197 511 Int.Cl. ..G0lnl/10,GOln21/24 [58] Field of Search 356/39, 181,196, 197,

[56] References Cited UNITED STATES PATENTS 3,555,284 l/197l Anderson 356/246 Primary Examiner-Vicent P. McGraw Attorney, Agent, or Firm-John A. Horan; David S. Zachry; Stephen D. Hamel [5 7] ABSTRACT A rotor design which provides improved mixing of sample and reagent liquids in a fast photometric analyzer of the rotary cuvette type is described. According to the preferred embodiment, one or more ramp-like projections are provided along the wall of each sample analysis cuvette to enhance mixing.

2 Claims, 2 Drawing Figures PATENTED NIB 1 31974 3,829 9223 MIXING ROTOR FOR FAST ANALYZER OF ROTARY CUVETTE TYPE WITH MEANS FOR ENHANCING THE MIXING OF SAMPLE AND REAGENT LIQUIDS BACKGROUND OF THE INVENTION The invention described herein relates generally to photometers and more particularly to an improved rotor for fast analyzers of the rotary cuvette type characterized by improved mixing of sample and reagent liquids in its sample analysis cuvettes. It was made by an employee of the US. Atomic Energy Commission in the course of his employment.

The general design and operation of fast photometric analyzers of the rotary cuvette type are generally described in US. Pat. No. 3,555,284, issued Jan. 12, 1971, to common assignee in in the name of Norman G. Anderson. In the analyzer described in that patent, a central loading disk is provided for statically receiving sample and reagent liquids prior to a photometric analysis operation. The sample and reagent liquids are then brought together dynamically by rotation induced forces in a separate mixing chamber or directly in respective sample analysis cuvettes. Complete and rapid mixing of the sample and reagent liquids is essential to ensure accurate photometric analysis of the cuvette contents.

Several techniques inlcuding tangential insertion of sample and reagent liquids into the cuvettes,the drawing of air bubbles through the cuvettes, rapid rotor acceleration and decleration, and parallel transfer of sample and reagent liquids have been used to enhance mixing with some degree of success. However, the recent introduction of miniaturized fast analyzers with correspondingly miniaturized rotors and sample analysis cuvettes, loading cavities, and sample and reagent volumes has resulted in decreased turbulent mixing because of the relatively increased effects of surface tension on the fluid flow of small liquid volumes.

It is, accordingly, a general object of the invention to provide a rotor design for a fast analyzer of the rotary cuvette type whereby sample and reagent mixing is enhanced.

Another object of the invention is to provide a rotor design for a fast analyzer of the rotary cuvette type wherein the sample analysis cuvettes are designed to enhance mixing.

SUMMARY OF THE INVENTION A rotor design characterized by improved mixing of sample and reagent liquids is provided for fast analyzers of the rotary cuvette type. At least one ramp-like projection is provided along the wall of each sample analysis cuvette to induce mixing of sample and reagent liquids upon rotation of the rotor. The use of such projections eliminates the need for supplemental mixing techniques and is especially desirable in miniature rotors where surface tension effects seriously inhibit turbulent mixing.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a top plan view of a rotor made in accordance with the invention.

FIG. 2 is a vertical section view of the rotor of FIG. 1.

DESCRIPTION OF THE PREFERRED EMBODIMENT Referring to the drawings, a typical single sample analysis station 1 comprises two sample and reagent static loading cavities 2 and 3 serially connected by means of passages 4 and 5 with each other and with a sample analysis cuvette 6. As shown in FIG. 2, the rotor is of laminated construction with a central opa ue disk 7 sandwiched between transparent plates 8 an 9. Apertures 11 and 12 in plate 8 facilitate static loading of cavities 2 and 3 prior to dynamic transfer of sample and reagent liquids from those cavities to respective cuvettes 6. Other loading cavity arrangements such as described in copending application Ser. No. 203,248, filed Nov. 30, 1971 and now US. Pat. No. 3,744,974, of common assignee maybe used without departing from the invention, which is restricted, rather, to an improved sample analysis cuvette design characterized by improved mixing of sample and reagent liquids discharged thereto.

As shown in the top view of FIG. 1, each cuvette 6 is provided with at least one axially and radiall extending ramplike projection 13. A preferred con lguration of projections 13 incorporates generally concave side portions which effectivelg direct liquid in the cuvette inward as shown by the roken flow arrows 14. Such flow effectively mixes the contents of cuvettes 6 by forcing liquid near the cuvette walls inward.

In theory, the mixing action depends upon the inherent relative rotation which develops between the cuvette walls and liquid contained therein during and briefly following acceleration of the rotor which defines the sample analysis cuvettes. The magnitude of this relative rotation decreases to a minimum following initial acceleration of the rotor since the cuvette contents are also accelerated by the propellin effect of the confining cuvette walls until it approaches the rotational speed of the cuvette walls with the rotor operating at constant speed. Acceleration and deceleration of the rotor will increase the mixing action of projections 13 by causing further relative rotation of the cuvette walls and the cuvette contents.

The foregoing description of one embodiment of the invention is offered for illustrative purposes only and should not be interpreted in a strictly limiting sense. For example, sample and reagent loading arrangements other than the one shown may be used without departing from the scope of the invention. Also, more than one projection per sample analysis cuvette may be used. It is intended, rather, that the invention be limited only by the scope of the claims attached hereto.

What is claimed is:

1. In a rotor for use in a fast photometric anal zer of the rotary cuvette type comprising a disk-shape member of laminated construction with a central opaque disk sandwiched between top and bottom transparent walls, and wherein said disk-shaped member defines a circular array of sample analysis cuvettes extending axially through said central opaque disk and means for loading and injecting sample and reagent liquids into said sample analysis cuvettes; the improvement wherein each of said sample analysis cuvettes has a generally cylindrical side wall defined by said opaque disk with a projection extending axially along said side wall between said top and bottom transparent walls and radially inward toward the center of said cuvette for enhancing the mixing of sample and reagent liquids therein.

2. The improvement of claim I wherein said projection defines two axially extending, intersecting, concave surfaces, each having a radius of curvature which is smaller than that pf s aid angple analysis cuvette.

Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US3890101 *Feb 15, 1974Jun 17, 1975Us EnergyCollection ring for use in multiple-sample blood fractionation centrifugal rotors
US3953172 *May 10, 1974Apr 27, 1976Union Carbide CorporationBlood, centrifuging
US4043678 *Mar 1, 1976Aug 23, 1977Technicon Instruments CorporationCuvette
US4123173 *Jun 9, 1976Oct 31, 1978Electro-Nucleonics, Inc.Rotatable flexible cuvette arrays
US4202665 *Nov 6, 1978May 13, 1980Albert Einstein College Of Medicine Of Yeshiva University A Division Of Yeshiva UniversityDetection of hepatitis B surface antigen
US4225558 *Sep 19, 1978Sep 30, 1980Honeywell Inc.Radially-spaced recessed tray
US4226531 *Aug 29, 1977Oct 7, 1980Instrumentation Laboratory Inc.Disposable multi-cuvette rotor
US4239853 *Jan 22, 1979Dec 16, 1980Bradley Rex LAntibiotic testing method and apparatus having a channelized reservoir
US4254084 *Dec 13, 1978Mar 3, 1981Blum Alvin SOptical cell for photometric analysis
US4279862 *Nov 16, 1978Jul 21, 1981Bretaudiere Jean PierreCentrifugal photometric analyzer
US4314970 *Aug 27, 1980Feb 9, 1982Instrumentation Laboratory Inc.Rotor assemblies for use in photometric centrifugal analyzers
US4373812 *Mar 25, 1981Feb 15, 1983Instrumentation Laboratory Inc.Cuvette assembly
US4468371 *Jul 19, 1982Aug 28, 1984Daryl Laboratories, Inc.Immunoassay test slide
US4557600 *Aug 30, 1982Dec 10, 1985Boehringer Mannheim GmbhProcess and device for the control and mixing of a fluid current subjected to centrifugal force
US4629703 *Jan 14, 1983Dec 16, 1986Technicon Instruments CorporationAutomated analytical system
US4883763 *Sep 30, 1988Nov 28, 1989Abbott LaboratoriesSample processor card for centrifuge
US4894204 *Dec 9, 1988Jan 16, 1990InovelfRotor with dynamic pipeting for a centrifuge analysis device
US4999304 *Dec 28, 1987Mar 12, 1991Miles Inc.Analyzing blood and other body fluids
US5071625 *Jan 20, 1988Dec 10, 1991Fisher Scientific CompanyRotor; circumferential array; interlock structure; aligned orientation; air circulation; centrifugal analyzers
US5627041 *Sep 2, 1994May 6, 1997Biometric Imaging, Inc.Fluid flow of sample, receivers and centrifuging
US5693233 *Dec 4, 1995Dec 2, 1997AbaxisMethods of transporting fluids within an analytical rotor
US8486333Jan 16, 2009Jul 16, 2013Micropoint Biosciences, Inc.Centrifugal fluid analyzer rotor
USRE30391 *Feb 23, 1976Sep 2, 1980Abbott LaboratoriesChemical analysis cuvette
DE4006498A1 *Mar 2, 1990Jan 24, 1991Gen ElectricLamellendichtungen
DE4006498C2 *Mar 2, 1990Apr 1, 1999Gen ElectricVorrichtung zum Abdichten eines Raumes in einer Turbomaschine
Classifications
U.S. Classification356/246, 356/427, 250/576, 422/72, 422/549
International ClassificationG01N21/07, B01F13/00
Cooperative ClassificationB01F2215/0037, G01N21/07, B01F13/0016
European ClassificationG01N21/07, B01F13/00K