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Publication numberUS3853864 A
Publication typeGrant
Publication dateDec 10, 1974
Filing dateDec 7, 1972
Priority dateDec 7, 1972
Also published asCA1017341A1, DE2360329A1
Publication numberUS 3853864 A, US 3853864A, US-A-3853864, US3853864 A, US3853864A
InventorsA Santilli, A Scotese
Original AssigneeAmerican Home Prod
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
1,7-dialkyl-1,2-dihydro-4-hydroxy-1,8-naphthyridine-3-carboxylic acid alkyl esters
US 3853864 A
Abstract
1,7-Dialkyl-1,2-dihydro-4-hydroxy-1,8-naphthyridine-3-carboxylic acid alkyl esters are prepared by cyclization of 2-[(2-alkoxycarbonylethyl)alkylamino]-6-alkylnicotinic acid alkyl esters. The compounds so produced exhibit in vivo antibacterial activity.
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Description  (OCR text may contain errors)

United States Patent 191 Santilli et al.

[451 Dec. 10, 1974 1,7-DIALKYL-1,2-DIHYDRO-4-HYDROXY- 3,786,043 1/1974 Brundage et a1. 260/2955 B 1 N HT Y 3 0 3,813,406 5/1974 Wada et al. 260/2955 B ACID ALKYL ESTERS o OTHER PUBLICATIONS [75] Inventors: Arthur A. Santilh, l-Iavertown;

Anthony Scmese, King of Prussia Lesher et al., Journal of Medicinal And Pharmaceutiboth of cal Chemistry, vol. 5, N0. 5, Pages 1063-1065, Sept. [73] Assignee: American Home Products 1962 RS] Corporation, New York, NY.

[22] Filed: Dec. 7, 1972 Primary Examiner-Alan L. Rotman 1 pp No: 313,048 Attorney, Agent, or Firm-David E. Frankhouser [52] U.S. Cl 260/2955 B, 260/2955 R, 57 ABSTRACT 260/294.8 F,424/266 [51] hit. Cl C07c 31/36 1 7 1 1 1 2 i 4 1 [58] Field of Search 260/2955 B 3 carboxylic acid alkyl esters are prepared by cycliza tion of 2-[(2-alkoxycarbonylethyl)alkylamin01-6- alkylnicotinic acid alkyl esters. The compounds so References C'ted produced exhibit in vivo antibacterial activity.

UNITED STATES PATENTS 3,590,036 6/1971 Lesher et a1. 260/2955 B 8 Claims, 1 Drawing Figure 9. 3 e COR 0 COR I HNCH CH E0R I 2 it 9 R N Y R N NCH CH COR I[ 111 II COR R N u R PAIENI an: 1 0 m4 m ow zo zoz z N mmow Alllllll 1 1,7-DIALKYL-1,2-DIHYDRO-4-HYDROXY-l,8- NAPHTHYRIDINE-S-CARBOXYLIC ACID ALKYL ESTERS This invention relates to new and pharrnacologically active chemical compounds classified in the art of chemistry as alkyl esters of l,7-dialkyl-1,2-dihydro-4- hydroxy- 1 ,8-naphthyridine-3-carboxylic acids.

1 ,7-Dialkyl-l ,4-dihydro-4-oxo- 1 ,8-naphthyridine-3- carboxylic acids and esters thereof are described in U.S. Pat. No. 3,590,036 and in an article by G. Lesher et al. J. Med. Pharm. Chem, 5, 1063 (1962).

The invention sought to be patented in its broadest aspect comprises chemical compounds of the structural formula I COR1 \N N wherein R is a straight-chain lower alkyl group, R and- R are each a straight-chain or branched lower alkyl group, and M is hydrogen or an alkali metal, and, when M is hydrogen, the non-toxic, pharmaceutically acceptable acid addition salts thereof.

Preferred embodiments of the compounds of Formula I are those wherein R is ethyl, R is methyl, R is a straight-chain or branched lower alkyl group, and M is hydrogen.

The compounds of Formula I exhibit in vivo antibacterial effects as demonstrated by evaluation in standard in vivo anti-bacterial test procedures.

In describing the invention herein, reference will be made to the annexed Drawing which shows the preparation of the l,7-di(1ower)alkyl-1,2-dihydro-4- hydroxy-l,8-naphthyridine-3-carboxylic acid, (lower- )alkyl esters. In the Drawing, R, R and R have the meanings hereinbefore set forth in Formula I, M is an alkali metal, and Y is chloro, bromo, iodo, benzenesulfonyloxy, p-methylbenzenesulfonyloxy, or pbromobenzenesulfonyloxy.

Referring now to the Drawing, where the compounds are assigned Roman numerals for identification, the compounds of the invention are prepared according to the following methods:

In the first step, a nicotinic acid ester (II), having a displaceable substituent in the 2-position, is reacted with a 3-[N-alkylamino1propionic acid ester (III) to produce a nicotinic acid ester compound (IV) having an (alkoxycarbonylethyl)alkylamino substituent in the 2-position. The reaction is carried out in the presence of an acid acceptor, such as sodium carbonate, in'a reaction-inert solvent, such as dimethylformamide (DMF) or dimethyl-acetamide (DMA), at a temperature ranging from 150C to 175C. With DMF or DMA as solvents, it is convenient to perform the reaction at the reflux temperature.

In the second step, the Z-nicotinic acid ester compound (IV) is cyclized under Dieckmann reaction conditions to afford an alkyl 1,7-dialkyl-l,2-dihydro-4- hydroxy-l ,8-naphthyridine-3-carboxylate.

The cyclization reaction is preferably effected by heating the intermediate (IV) at a temperature ranging from 50C to 100C, (preferably 60C to 80C) in the presence of an alkali metal lower alkoxide in a nonreactive organic solvent, preferably a lower alkanol.

Under these conditions, the product is obtained as the alkali metal enolate salt (V). It will be appreciated that, to prevent transesterification, best results will be obtained when the alkoxide reagent and the alkanol employed as solvent'contain the same alkyl moiety (R that is present in the alkyl ester function at the 3- position of the cyclized product. The alkali metal alkoxide can conveniently be prepared in situ by dissolving the alkali metal in the desired alkanol solvent, and thereafter adding the intermediate to be cyclized.

The enol salt (V) can be converted to the enol (VI) by acidification with an acid whereby the alkali metal (M) is replaced by hydrogen. When a weak acid (i.e., one which will not protonate the amine function) is employed, the product is obtained in the form of the free amine. If desired, the free amine can be converted to an acid addition salt by treatment with a strong acid. The acid employed for formation of the acid addition salt must be non-toxic and acceptable for pharmaceutical use. Among the suitable acids are hydrochloric, hydrobromic, sulfuric, nitric, sulfonic, benzenesulfonic, p-toluenesulfonic, methanesulfonic, or phosphoric.

As used herein and in the appended claims, lower alkyl means an alkyl group having from one to six carbon atoms. The term lower alkoxide means an alkoxy group having from one to six carbon atoms. Alkali metal means sodium or potassium.

The starting compounds for the processes described herein are either known compounds or can be prepared from known compounds by procedures well known in the art. 4

It will be appreciated by those skilled in the art that the compounds of the invention can exist as tautomers. Hence, the compounds can be depicted in the enol form as in Formula I or in the keto form as in Formula la:

Infrared and nuclear magnetic resonance spectrographic data indicate that the enol form (I) is the predominate form. Hence, the compounds of the invention are depicted and named herein in the enol form as 1,2- dihydro-4-hydroxyl ,8-naphthyridine-3-carboxylic acids rather than as l,2,3,4-tetrahydro-4-oxo-l ,8- naphthyridine-3-carboxylic acids. It is understood that both the enol and keto forms are equivalent for the purposes of the invention.

When the compounds of the invention are employed as in vivo anti-bacterial agents, they may be administered alone or in combination with pharmaceutically acceptable carriers, the proportion of which is determined by the solubility and chemical nature of the compound, chosen route of administration, and standard pharmaceutical practice. For example, they may be administered orally in the form of tablets or capsules containing such excipients as starch, lactose, magnesium stearate, and so forth. They may be administered orally in the form of solution or they may be injected parenterally, e.g. intramuscularly. For parenteral administration, they may be used in the form of a sterile solution or suspensions containing other solutes, for example, enough saline or glucose to make the solution isotonic.

The dosage of the present pharmacologically active agents will vary with the form of administration and the particular compound chosen. Furthermore, it will vary with the particular subject under treatment. Generally, treatment is initiated with small dosages substantially less than the optimum dose of the compound. T hereafter, the dosage is increased by small increments until the optimum effect under the circumstances is reached. It will generally be found that when the composition is administered orally, larger quantities of the active agent will be required to produce the same effect as a smaller quantity given parenterally. In general, the compounds of this invention are most desirably administered at a dosage that will generally afford effective results without causing any harmful or deleterious side effects.

The manner and processes for making and using the compounds of the invention are illustrated in the following examples, wherein all temperatures are expressed as degrees Centigrade.

EXAMPLE 1 2-Chloro-6-Methylnicotinic Acid Methyl Ester A mixture of 25 g. of 2-hydroxy-6-methyl nicotinic acid in 125 ml. of phosphorus oxychloride is heated under reflux for 2.5 hours. The phosphorus oxychloride is then removed in a rotary evaporator. The residue is slowly poured into 600 ml. of cold methanol. The methanol is evaporated and the residue dissolved in 250 ml. of water and extracted with 150 ml. of chloroform. The chloroform layer is dried over magnesium sulfate, filtered and evaporated to give 29.5 g. of the title compound as an oil.

Analysis for c H NClO Calculated: C, 51.77; H, 4.34; N, 7.55 Found: C, 51.92; H, 4.65; N, 7.04

7 EXAMPLE ll 2-[ 2-Ethoxycarbonylethyl )Ethylamino1-6-Methylnicotinic Acid Methyl Ester A mixture of 12 g. of 2-chloro-6-methylnicotinic acid, methyl ester, 0.5 g. of ethyl-3- (ethylamino)propionate [D. W. Adamson, J. Chem. Soc., 1949 Suppl. Issue 1 S 144] and 7 g. of sodium carbonate in 100 ml. of dimethylformamide (DMF) is heated under reflux for 18 hours. The mixture is filtered and the DMF evaporated. The residue is partitioned between 250 ml. of ether and 250 ml. of water. The ether layer is dried over magnesium sulfate, filtered, and evaporated to give an oil which is used directly in Example 111.

EXAMPLE III l-Ethyll ,2-Dihydro-4-Hydroxy-7-Methyl-1 ,8- Naphthyridine-Ii-Carboxylic Acid Ethyl Ester, Hydrochloride The oil from Example 11 is added to a sodium ethoxide solution prepared by dissolving 3.0 g. of sodium in 250 ml. of absolute ethanol. The mixture is heated at reflux temperature for minutes. The mixture is cooled. The insoluble material is collected and trimrated with 200 ml. of 20% aqueous acetic acid solution. The insolubles are collected and recrystallized from petroleum ether. The free base (mp. 7580) is dissolved in ethyl acetate and acidified with ethereal hydrochloric acid to yield 1.3 g. of the title compound, m.p. 161164. Analysis for C14H19N2ClO3 Calculated: C, 56.28; H, 6.41; N, 9.38

Found: C, 56.52; H, 6.54; N, 9.62

EXAMPLE IV 2-[ Z-n-Butoxycarbonylethyl )Ethylamino ]-6-Methylnicotinic Acid Methyl Ester A mixture of 18.5 g. of 2-chloro-6-methylnic0tinic acid methyl ester, 17.3 g. of butyl 3- ethylaminopropionate and 10.6 g. of sodium carbonate in ml. of dimethylformamide (DMF) is heated under reflux for 20 hours. The mixture is filtered and the DMF evaporated. The residue is partitioned between 250 ml. of ether and 250 ml. of water. The ether layer is dried over magnesium sulfate, filtered and evaporated to afford an oil which is used directly in Example V.

EXAMPLE V l-Ethyl-l ,2-Dihydro-4-Hydroxy-7-Methyl-l ,8- Naphthyridine-3-Carboxylic Acid Butyl Ester, Hydrochloride The oil from Example IV is added to a solution of so dium n-butoxide prepared by dissolving 2.3 g. of sodium in 400 ml. of butanol. The mixture is heated at reflux temperature for 10 minutes. The precipitate is collected and dissolved in ml. of 10% aqueous acetic acid. The solution is extracted with 150 ml. of ether. The ether layer is dried over magnesium sulfate, filtered, and diluted with 15 ml. of ethyl acetate. On acid ification of this solution with ethereal hydrochloric acid a precipitate separates. Recrystallization of the precipitate from ethyl acetate gives 0.8 g. of the title compound, m.p. 124-127.

Analysis for C H N O Cl Calculated: C, 58.80; H, 7.09; N, 8.57; Cl, 10.85 Found: C, 58.44; H, 7.16; N, 8.60; C], 10.96

EXAMPLE V1 2-[(Z-Ethoxycarbonylethyl )n-Butylaminol-(u-Methylnicotinic Acid Methyl Ester A mixture of 3.7 g. of 2-chloro-6-methylnicotinic acid methyl ester, 3.4 g. of ethyl 3- butylaminopropionate and 2.12 g. of sodium carbonate in 40 ml. of dimethylformamide (DMF) is heated under reflux for 6 hours. The mixture is filtered and the filtrate is partitioned between 500 ml. of water and 100 ml. of chloroform. The chloroform layer is dried over magnesium sulfate, filtered, and evaporated to give an oil which is used directly in Example Vll.

EXAMPLE VII l-Butyll ,2-Dihydro-4-Hydroxy 7-Methyll ,8- Naphthyridine-3-Carboxylic Acid Efliyl Ester, Hydrochloride The oil from Example Vl is added to a sodium ethoxide solution prepared by dissolving 0.46 g. of sodium in 50 ml. of ethanol. After warming for 5 minutes the mixture is filtered and the filtrate cooled in an ice bath. The precipitate thus formed is triturated with 100 ml. of 10% aqueous acetic acid. The insolubles are collected and dissolved in ethyl acetate. Acidification with ethereal hydrochloric acid gives a precipitate which is filtered. The filtrate is cooled in ice to afford a second crop of the title compound, m.p. l l21 18. Analysis for C1 H23N203Cl Calculated: C, 58.80; H, 7.09; N, 8.57

Found: C, 58.78; H, 6.99;-N, 8.60

EXAMPLE VllI 2-Benzenesulfonyloxy-6-Methylnicotinic Acid Methyl Ester To a solution of 0.45 g. of sodium in 150 ml. of ethanol is added 3.34 g. of 2-hydroxy-6-methylnicotinic acid methyl ester. After stirring for 30 minutes the insoluble material is collected and suspended in 100 m1. of N,N-dimethylformamide. To this suspension is added 3.53 g. of benzenesulfonyl chloride. The mixture is warmed on a steam bath for a few minutes and filtered. The filtrate is diluted with 100 ml. of water. On cooling, a precipitate develops. Recrystallization from benzenepetroleum affords the title compound, m.p. 6164.

Analysis for C H NO S Calculated: C, 54.71; H, 4.26; N, 4.56 Found: C, 54.55; H, 4.20; N, 4.31

EXAMPLE IX.

l-Ethyl-l ,2-Dihydro-4-Hydroxy-7-Methyl-l ,8- Napythyridine-3-Carboxylic Acid Ethyl Ester (Alternative Method) A stirred mixture of 3.07 g. of 2-benzenesulfonyloxy- -methylnicotinic acid, methyl ester, 1.45 g. of ethyl 3-ethyl-aminopropionate, and 1.06 g. of sodium carbonate in 25 ml. of N,N-dimethylformamide is heated under reflux for 6 hours. The mixture is filtered and the filtrate partitioned between 500 ml. if water and 100 ml. of ether. The ether layer is dried over magnesium sulfate, filtered and evaporated. The residue is added to a solution of 0.23 g. of sodium in 30 ml. of ethanol and is warmed to reflux temperature for a few minutes. The precipitate is collected and triturated with 50 ml. of 10% aqueous acetic acid to afford 1-ethyl-1,2- dihydro-4-hydroxy-7-methyl, l ,8-naphthyridine-3- carboxylic acid, ethyl ester. A mixture m.p. with the free base prepared in Example IIl showed no depression, and the infrared spectra of both samples are identical.

EXAMPLE X The in vivo therapeutic activity of the compounds of Formula I against bacterial infections is demonstrated and elicited by employing the following test procedure:

Forty male mice, weighing 18 g. i l g., are divided into four separate groups containing ten mice in each group. Each animal is injected intraperitoneally with 0.5 ml. of a standardized suspension of the infective agent in 5% gastric mucin. Six hours post infection, each mouse is given a single oral dose of the test compound.

The amount of compound given varies in each of the four groups; i.e., 4 graded doses are administered. The

animals are observed for 14 days and deaths recorded daily. The CD (curative dose) values are determined by acceptable statistical procedures. [See Reed and Muensch, Amer. J. of Hygiene, 27, 493 (1938)].

When tested as above-described, l-ethyl-l ,2- dihydro-4- hydroxy-7-methyl-1 ,8-naphthyridine-3-carboxylic acid ethyl ester hydrochloride showed therapeutic activity, as measured by CD values, against infections caused by the following gram-negative organisms at a dosage level in the range of 2 to 6 mg/mouse: Escherichia coli 920, Salmonella typhimurium SaB-l, Salmonella typhosa SaD-l 2, Proteus vulgaris 347, and Proteus mirabilis 3.

l-Ethyl- 1 ,2-dihydro-4-hydroxy-7-methyl-1 ,8- naphthyridine-3-carboxylic acid n-butyl ester, hydrochloride, showed activity (CD against Escherichia coli 920 at about 2 mg/mouse.

What is claimed is:

1. A compound of the formula wherein R is a straight-chain lower alkyl group, R and R are each a straight-chain or branched lower alkyl group, and M is hydrogen or an alkali metal, and, when M is hydrogen, the non-toxic, pharmaceutically acceptable acid addition salts thereof.

2. A compound as defined in Formula 1 wherein R is ethyl, R is methyl, R is a straight-chain or branched lower alkyl group, and M is hydrogen, and the nontoxic, pharmaceutically acceptable acid addition salts thereof.

3. A compound as defined in claim 2 which is l-ethyl- 1 ,2-dihydro-4-hydroxy-7-methyl-1,8-naphthyridine-3- carboxylic acid ethyl ester.

4. A compound as defined in claim 2 which is l-ethyll ,2-dihydro-4-hydroxy-7-methyl-l ,8-naphthyridine-3- carboxylic acid ethyl ester, hydrochloride.

5. A compound as defined in claim 2 which is l-ethyl- 1 ,2-dihydro-4-hydroxy-7-methyl-l ,8-naphthyridine-3- carboxylic acid butyl ester.

6. A compound as defined in claim 2 which is l-ethyll ,2-dihydro-4-hydroxy-7-methyl-1,8-naphthyridine-3- carboxylic acid butyl ester, hydrochloride.

7. A compound as defined in claim 1 which is lbutyl-l ,2-dihydro-4-hydroxy-7-methyll ,8-naphthyridine-3-carboxylic acid ethyl ester.

8. A compound as defined in claim 1 which is lbutyl-l ,2-dihydro-4-hydroxy-7-methyl-1 ,8-naphthyridine-3-carb0xylic acid ethyl ester, hydrochloride.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US3590036 *Nov 18, 1968Jun 29, 1971George Y LesherNaphthyridine-3-carboxylic acids,their derivatives and preparation thereof
US3786043 *Jun 19, 1972Jan 15, 1974Sterling Drug Inc7-methyl-4-chloro (or -4-lower-alkoxy)-1,8-naphthyridine-3-carboxylates
US3813406 *Feb 18, 1971May 28, 1974Koei Chemical CoProcess for preparing 1-alkyl-1,8-naphthyridine compounds
Non-Patent Citations
Reference
1 *Lesher et al., Journal of Medicinal And Pharmaceutical Chemistry, Vol. 5, No. 5, Pages 1063 1065, Sept. 1962 Rsl J5.
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US4128649 *Jun 29, 1977Dec 5, 1978Sandoz, Inc.4-Hydroxy-pyrido[2,3-b]pyridine-2(1H)-one-3-carboxylic acids and esters
US4273930 *Aug 9, 1979Jun 16, 1981Sandoz, Inc.4-Hydroxy-2-quinolinone-3-carboxylic acid esters
US4281131 *Jul 16, 1979Jul 28, 1981Sandoz, Inc.4-Hydroxy-2-quinolinone-3-carboxylic acids and salts thereof
Classifications
U.S. Classification546/123, 546/318, 546/310
International ClassificationA61P31/04, C07D471/04, A61K31/435, C07D213/80
Cooperative ClassificationC07D471/04, C07D213/80
European ClassificationC07D213/80, C07D471/04