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Publication numberUS3901658 A
Publication typeGrant
Publication dateAug 26, 1975
Filing dateJul 30, 1974
Priority dateJul 30, 1974
Publication numberUS 3901658 A, US 3901658A, US-A-3901658, US3901658 A, US3901658A
InventorsCarl A Burtis, Wayne F Johnson
Original AssigneeUs Energy
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Whole blood analysis rotor assembly having removable cellular sedimentation bowl
US 3901658 A
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Description  (OCR text may contain errors)

United States Patent [1 1 Burtis et al.

[ WHOLE BLOOD ANALYSIS ROTOR ASSEMBLY HAVING REMOVABLE CELLULAR SEDIMENTATION BOWL [75] Inventors: Carl A. Burtis, Knoxville; Wayne F.

Johnson, Loudon, both of Tenn.

[73] Assignee: The United States of America as represented by the United States Energy Research and Development Administration, Washington, DC.

22 Filed: July 30,1974

21 Appl.No.:493,006

[52] U5. Cl. 23/259; 233/26; 356/39; 356/246 [51] Int. Cl. 1. B04B 5/12; GOlN 33/16; G01N 21/00; GOlN 1/10 [581 Field of Search 23/253 R, 259; 356/39, 356/197, 246; 233/26 [56] References Cited UNITED STATES PATENTS Maddox et al 23/259 51 Aug. 26, 1975 Mailen 23/259 Mailen 23/259 X Primary Examiner-Morris O. Wolk Assistant ExaminerTimothy W. Hagan Attorney, Agent, or FirmDean E. Carlson; David S. Zachry; Stephen D. Hamel 7 ABSTRACT A rotor assembly for performing photometric analyses using whole blood samples. Following static loading of a gross blood sample within a centrally located, removable, cell sedimentation bowl, the red blood cells in the gross sample are centrifugally separated from the plasma, the plasma displaced from the sedimentation bowl, and measured subvolumes of plasma distributed to respective sample analysis cuvettes positioned in an annular array about the rotor periphery. Means for adding reagents to the respective cuvettes are also described.

7 Claims, 1 Drawing Figure WHOLE BLOOD ANALYSIS ROTOR ASSEMBLY HAVING REMOVABLE CELLULAR SEDIMENTATION BOWL BACKGROUND OF THE INVENTION The invention described herein relates generally to photometers and more particularly to an improved whole blood analysis rotor assembly for a multi-station dynamic photometer of the rotary cuvette type. It was made in the course of, or under, a contract with the U.S. Atomic Energy Commission.

Fast photometric analyzers incorporating multistation rotary cuvette systems are becoming widely used in various laboratories because of their ability to rapidly and accurately analyze large numbers of samples. Of particular interest are blood tests including glucose, LDl-I, SGOT, SGPT, BUN, total protein, alkaline, phosphatase, bilirubin, calcium, chloride, sodium, potassium, and magnesium. Since such tests are normally performed on blood plasma, blood cells must be removed from whole blood samples prior to analysis. Cuvette rotors designed to accept and automatically process whole blood samples must, therefore, be capable of separating plasma from cellular material. In addition, such rotors must be designed for receiving a sample in a loading operation, measuring discrete subvolumes of separated plasma from each sample analysis cuvette and transferring the subvolumes into respective cuvettes.

One rotor assembly which has been designed to accept and automatically process whole blood samples is described in copending application Ser. No. 489,305 of common assignee. That rotor is difficult to clean since red cells are closely packed within capillary sized passageways during a centrifugal separation operation de signed to separate the plasma and cellular components. Also, because of its design which requires that part (about half) of a sample be wasted, blood volumes are required greatly in excess of that used in the actual analyses.

It is, accordingly, a general object of the invention to provide an improved rotor for a multi-station photometric analyzer which is suitable for use in performing whole blood analyses.

Another more particular object of the invention is to provide an improved rotor for a multi-station photometric analyzer suitable for receiving a whole blood sample, centrifuging the whole blood sample to separate it into cellular and plasma components, measuring discrete plasma subvolumes, and transferring the subvolumes to respective sample analysis cuvettes.

Another particular object of the invention is to provide an improved rotor for a multi-station photometric analyzer suitable for receiving a whole blood sample wherein sedimented cellular components are readily removable following sample analysis.

Still another object of the invention is to provide an improved rotor for a multi-station photometric analyzer suitable for receiving a whole blood sample wherein the volume of blood required for analysis is minimized.

Other objects of the invention will be apparent from an examination of the following written description of the invention and the appended drawings.

SUMMARY OF THE INVENTION In accordance with the invention, an improved rotor assembly is provided for use in performing whole blood analyses in a multi-station photometric analyzer. Included in the rotor assembly is a generally disk-shaped main rotor body and a removable sedimentation bowl nested within the main rotor body and adapted to rotate with that body as a unit. Features defined by the main rotor body include: an annular plasma distribution manifold for receiving plasma displaced from the sedimentation bowl, volume measuring chambers and passageways for receiving plasma from the distribution manifold, means for receiving plasma overflow from the distribution manifold, sample analysis cuvettes disposed in a circular array about the rotor periphery and means for loading reagents into the sample analysis cuvettes. The removable sedimentation bowl includes a hollow disk-shaped base portion and an upstanding annular neck portion through which whole blood samples are statically loaded into the base portion and through which separated plasma is displaced under dynamic operating conditions. Using the subject improvedrotor assembly, cellular components are removed from whole blood samples and retained in the sedimentation bowl which can be cleaned between operations or disposed of and replaced with a new bowl.

DESCRIPTION OF THE PREFERRED EMBODIMENT Referring now to the drawing, which is a top, cut away, isometric view of a rotor assembly made in accordance with the invention, the rotor assembly is seen to include a sedimentation bowl 1 and a disk-shaped main rotor body 2. The sedimentation bowl is shaped to, nest concentrically within the rotor body at the level of the rotor body base and to rotate upon a turntable (not shown) with the rotor body as a unit. As shown, the sedimentation bowl comprises a shallow, hollow, disk-shaped base portion 3 and an upstanding annular neck portion 4 through which whole blood samples may be statically loaded into the base portion and separated plasma displaced under dynamic operating conditions. Neck portion 4 is provided with a slightly tapered inner surface having a larger diameter at its end which is fixed to base portion 3 to ensure movement of displacing liquid into the base portion during a plasma displacing operation. The main rotor body in the preferred embodiment is a vertically stacked, laminar construction comprising a base 5, a divider plate 6, a chamber plate 7, and capping plate 8. The base plate 5 is an annulus of transparent material which provides lower windows for the sample analysis cuvettes which is sized to provide an opening for receiving sedimentation bowl 1. Divider plate 6 is annular shaped and extends centripetally beyond the inner periphery of the base 5, permitting upward projection of the neck portion 4 therethrough. The divider plate 6 functions as a vertical retainer for the sedimentation bowl and as a lower wall for an annular plasma distribution manifold 9 formed in the centripetal region between divider plate 6 and a tapered portion of the chamber plate 7. i

The chamber plate 7 is, in comparison with the outer laminations, a thick annulus which provides a matrix defining the main functional chambers and inner connecting passageways as described below. The annular plasma distribution manifold 9 is formed by relieving a shallow conical portion of the matrix from the lower centripetal edge of the chamber plate. Plasma distribution manifold 9 extends from axially below to axially above the upper extremity of neck portion 4 when, as

shown, sediment bowl 1 is fully inserted within the main rotor body in operating position. A multiplicity (only one shown) of plasma volumetric measuring chambers are disposed in a circular array and displaced axially above and radially overlapping the distribution manifold 9. Plasma inlet ports 11 extend axially and provide liquid communication between distribution manifold 9 and each measuring chamber 10 to permit passage of plasma from the plasma distribution manifold to the respective measuring chambers. Ports 11 are precisely located on a common radius to facilitate equal filling of chambers 10 under centrifugal conditions. The measuring chambers 10 are vented to manifold 9 by means of vent ports 12 located centripetal to the plasma inlet ports 11.

A circular array of sample analysis cuvettes 13 is located peripherally within the divider plate 6 and chamber plate 7. Sample analysis cuvettes 13 are equal in number to, and are somewhat angularly offset from respective plasma measuring chambers 10. One reference cuvette, which is not in communication with a plasma measuring chamber 10, may be provided for photometric blank solutions. Corresponding measuring chambers 10 and sample analysis cuvettes 13 are in communication through corresponding folded passageways 14 which extend from the centrifugal extremity of each measuring chamber 10 and the centripetal extremity of each sample analysis cuvette 13. Each passageway 14 comprises three interconnected, radially extended segments which describe an N shaped path with a first segment 14a extending from the measuring chamber radially outward to a point about equal to the radius at which sample analysis cuvettes 13 are disposed, a second segment 14b extending radially inward from the centrifugal end of the first segment to a point centripetal to the circle upon which commonly lie the plasma inlet ports 11, and a third segment 14c extending radially outward from the centripetal end of the second segment to a sample analysis cuvette 13. At least one plasma overflow chamber 15 is defined within the matrix of the divider plate 6 in a generally peripheral location. The overflow chamber 15 is in communication with plasma distribution manifold 9 by means of an overflow passageway 16 which enters the distribution manifold 9 at a point just centripetal to the circle upon which lie plasma inlet ports 1 l. Overflow passage way 16 extends from plasma distribution manifold 9 upward to the top of the chamber plate 7, and then centrifugally to enter the overflow chamber 15.

As shown, each sample analysis cuvette 13 is provided with a cleanout passageway 17 extending from its lower centripetal extremity to the cavity which is formed in base 5 upon removal of sedimentation bowl 1 from its operating position. Plasma overflow chamber 15 may likewise be provided with a cleanout passageway.

Capping plate 8 is superimposed on chamber plate 7 partially to provide a top closure for the measuring chambers 10, sample analysis cuvettes 13, passageways 14, plasma overflow chamber 15 and overflow passageways 16. The capping plate also provides a matrix for forming reagent loading ports 18 (only 1 shown) which permit direct loading access to each sample analysis cuvette from the topside of the rotor assembly. Second cleanout passageways 19 extend between the reagent loading ports 18 and the centripetal extremity 20 of annular capping plate 8 to provide for fluid cleaning of the sample analysis cuvettes as do the first cleanout passageways 17.

In operation, diverse test reagents are pipetted into the sample analysis cuvettes 13 through the reagent loading ports 18 while the rotor is kept stationary. Whole blood is statically loaded within sedimentation bowl 1 and then centrifuged at about 4000 RPM to sediment cellular components in the periphery of the hollow base portion of the bowl. A comparatively dense, water immiscible liquid, e.g., a halocarbon oil, is added to the sedimented blood under the same dynamic conditions to displace plasma centripetally and upwardly through the neck portion 4 of the sedimentation bowl. The volume of displacing liquid is predetermined to slightly exceed the total volume of the measuring system defined by chambers 10 and passageways 14, in order that the measuring system will fill, yet not overflow in volume exceeding that of the overflow chamber 15. The displaced plasma spills over the top of neck portion 4 and is caught within distribution manifold 9. The plasma then passes through inlet ports 11 into plasma measuring chambers 10 and corresponding passageways 14 until it reaches the limiting centripetal level as defined by plasma overflow passagway 16. Excess plasma flows through overflow passageway 16 into the overflow chamber 15. The thus measured plasma subvolumes are displaced from the measuring chambers 10 and passageways 14 into respective sample analysis cuvettes by intermittent application of air pressure to the open center portion of capping plate 8, while maintaining rotation of the entire rotor assembly at about 1000 RPM. It is necessary to predetermine the combined volume of reagent and plasma samples to be sufficient for photometric measurement, without overfilling the sample analysis cuvettes to the point where liquid could be lost by way of the cleanout passageways 17.

The above described preferred embodiment and method of operation is intended to be illustrative and should not be interpreted in a limiting sense. For example, particulate suspensions other than whole blood could be processed to remove particulates and the clarified supernatant analyzed. Also, the particular manner in which reagents are loaded into the sample analysis cuvettes could differ from that illustrated in that separate reagent loading cavities could be provided which communicate by means of suitable passageways with respective sample analysis cuvettes. It is intended rather, that the invention be limited in scope only by the following claims.

What is claimed is:

1. A rotor assembly for a photometric solution analyzer of the rotary cuvette type suitable for use in analyzing whole blood samples comprising:

a. a generally disk-shaped main rotor body defining:

i. an annular plasma distribution manifold;

ii. a plurality of volume measuring chambers distributed in a circular array, said volume measuring chambers being in liquid flow communication with said plasma distribution manifold;

iii. means limiting the centripetal level of plasma in said volume measuring chambers during operation of said rotor;

iv. a plurality of sample analysis cuvettes disposed in a circular array about the periphery of said main rotor body, said sample analysis cuvettes being in liquid communication with said volume measuring chambers; and

v. means for loading reagents into said sample analysis cuvettes; and

b. a sedimentation bowl nested within said main rotor body and adapted to rotate with that body as a unit,

said sedimentation bowl comprising:

i. a hollow disk-shaped base portion, said base portion having a centrally located top opening for receiving whole blood samples and discharging displaced plasma; and

ii. an upstanding, open-ended, annular neck portion integrally fixed to said base portion in register with said top opening, the top end of said neck portion terminating within the center of said annular plasma distribution chamber within a plane axially intermediate to the axial extremities of such chamber.

2. The rotor assembly of claim 1 wherein said sedimentation bowl is removably nested within said main rotor body.

3. The rotor assembly of claim 1 further including a plurality of passageways communicating between said sample analysis cuvettes and said volume measuring chambers, each of said passageways comprising three radially extending interconnected passageway segments; a first segment extending radially from a respective sample analysis cuvette to a point centripetal to the, centripetal ends of said volume measuring chambers, a

second segment extending from the centripetal end of said first segment to a point centrifugal to said volume measuring chambers, and a third segment extending from the centrifugal end of said second segment to the centrifugal end of a respective volume measuring chamber.

4. The rotor assembly of claim 1 wherein said volume measuring chambers are axially displaced from said plasma distribution manifold with the centripetal ends of said volume measuring chambers radially overlapping the centrifugal extremity of said plasma distribution manifold.

5. The rotor assembly of claim 4 wherein axially extending plasma inlet ports communicate between said plasma distribution manifold and respective volume measuring chambers, said inlet ports being disposed on a common radius about the center of rotation of said rotor assembly.

6. The rotor assembly of claim 5 wherein said means limiting the centripetal level of plasma in said volume measuring chambers comprises a plasma overflow chamber and a plasma overflow passageway communicating between said plasma distribution manifold and said plasma overflow chamber.

7. The rotor assembly of claim 6 wherein said overflow passageway communicates with said plasma distribution manifold at a radius centripetal to the common radius on which said plasma inlet ports are disposed.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US3744974 *Nov 30, 1971Jul 10, 1973Atomic Energy CommissionLoading disk for photometric analyzer of rotary cuvette type
US3744975 *Dec 9, 1971Jul 10, 1973Atomic Energy CommissionRotor for multistation photometric analyzer
US3795451 *Apr 24, 1973Mar 5, 1974Atomic Energy CommissionRotor for fast analyzer of rotary cuvette type
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US4030888 *Feb 17, 1976Jun 21, 1977Toa Medical Electronics Co., Ltd.Automatic blood analyzer
US4035156 *Jan 21, 1977Jul 12, 1977The United States Of America As Represented By The United States Energy Research And Development AdministrationFilter type rotor for multistation photometer
US4256696 *Jan 21, 1980Mar 17, 1981Baxter Travenol Laboratories, Inc.Photometric analyzer, chromium, copper, alloy
US4274885 *Jul 19, 1979Jun 23, 1981Swartout Bobbye JMethod for washing centrifugal analyzer test disks
US4284602 *Dec 10, 1979Aug 18, 1981Immutron, Inc.Medical centrifuge
US4385115 *Oct 22, 1980May 24, 1983Hoffmann-La Roche Inc.Self contained microorganism analysis
US4431606 *May 1, 1981Feb 14, 1984Hoffmann-La Roche Inc.Multicuvette rotor for analyzer
US4469793 *Apr 7, 1982Sep 4, 1984Jean GuiganFor chemical reaction
US4576796 *Jan 18, 1984Mar 18, 1986Pelam, Inc.Centrifugal tissue processor
US4656009 *Sep 27, 1984Apr 7, 1987Le Materiel BiomedicalReaction support incorporating multiple recipients for testing liquid doses
US4663296 *Oct 26, 1983May 5, 1987Hoffmann-La Roche Inc.Multicuvette rotor for analyzer
US4756884 *Jul 1, 1986Jul 12, 1988Biotrack, Inc.For detecting presence of analyte in physiological fluid sample
US4817453 *Jan 22, 1988Apr 4, 1989E. I. Dupont De Nemours And CompanyFiber reinforced centrifuge rotor
US4835106 *Jul 17, 1987May 30, 1989Martin Marietta Energy Systems, Inc.Rotor for processing liquids using movable capillary tubes
US4963498 *Jan 15, 1988Oct 16, 1990BiotrackDetection of analyte by flow change after interaction with reagent
US5061381 *Jun 4, 1990Oct 29, 1991Abaxis, Inc.Distribution of separated plasma into differnt test wells with in centrifugal rotor for testing without transferring
US5160702 *Jan 17, 1989Nov 3, 1992Molecular Devices CorporationAnalyzer with improved rotor structure
US5173193 *Apr 1, 1991Dec 22, 1992Schembri Carol TCentrifugal rotor having flow partition
US5186844 *Apr 1, 1991Feb 16, 1993Abaxis, Inc.Separation chamber located radially outward from sample chamber and connected by flow restrictive channel
US5242606 *Oct 29, 1991Sep 7, 1993Abaxis, IncorporatedSample metering port for analytical rotor having overflow chamber
US5286454 *Apr 25, 1990Feb 15, 1994Nilsson Sven ErikCavity for taking up fluid by capillary action alone and at least one more requiring the application of centrifugal force
US5300779 *Aug 18, 1992Apr 5, 1994Biotrack, Inc.Capillary flow device
US5610074 *Feb 24, 1993Mar 11, 1997Beritashvili; David R.Centrifugal method and apparatus for isolating a substance from a mixture of substances in a sample liquid
US5650334 *Aug 31, 1995Jul 22, 1997First Medical, Inc.Labels on targets, polysaccharides and fluorescent dyes
US6299839Aug 31, 1995Oct 9, 2001First Medical, Inc.Mounted on the carriage and can be translated among a sample dispensing station, a fluid dispensing station, and a label detection zone.
US6692701 *Dec 9, 2002Feb 17, 2004V & P Scientific, Inc.Microarrayer
US6743632 *Mar 14, 2001Jun 1, 2004Universities Space Research AssociationCentrifugal analysis of stained samples; obtain slide, insert into container, apply force, monitor sample for staining pattern
US7026131Nov 15, 2002Apr 11, 2006Nagaoka & Co., Ltd.Methods and apparatus for blood typing with optical bio-discs
US7033747Apr 11, 2002Apr 25, 2006Nagaoka & Co., LtdApparatus for use in the detection of preferential targets in sample
US7061594Nov 9, 2001Jun 13, 2006Burstein Technologies, Inc.Disc drive system and methods for use with bio-discs
US7087203Nov 19, 2001Aug 8, 2006Nagaoka & Co., Ltd.Blood classification of humans; obtain erythrocytes, incubate with antibody, detect bound cells and classify
US7157049Nov 13, 2002Jan 2, 2007Nagaoka & Co., Ltd.Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
US7200088Jan 10, 2002Apr 3, 2007Burstein Technologies, Inc.System and method of detecting investigational features related to a sample
US7221632Jul 12, 2002May 22, 2007Burstein Technologies, Inc.Optical disc system and related detecting methods for analysis of microscopic structures
US7390464Jul 26, 2004Jun 24, 2008Burstein Technologies, Inc.Rotationally controlled liquid valves used independently or in combination with air chambers for pneumatic fluid displacement allowing sample isolation
US7582049 *Feb 8, 2006Sep 1, 2009Caridianbct, Inc.Fluid separation devices, systems and/or methods using a centrifuge and roller pump
US7582472Jul 26, 2004Sep 1, 2009Smith Kenneth EDevice which utilizes capillary flow for partitioning a liquefied sample into discrete volumes; detection and quantification of biological materials, microorganisms, and analytes in fluids
US7914753Dec 2, 2008Mar 29, 2011Industrial Technology Research InstituteAnalytical system, and analytical method and flow structure thereof
USRE30391 *Feb 23, 1976Sep 2, 1980Abbott LaboratoriesChemical analysis cuvette
EP0039825A1 *Apr 29, 1981Nov 18, 1981F. HOFFMANN-LA ROCHE & CO. AktiengesellschaftCuvette rotor for analyzer and method of operation of said cuvette rotor
EP0062907A1 *Apr 8, 1982Oct 20, 1982Jean GuiganMethod and apparatus for delivering a predetermined amount of sample liquid to a cell
WO1990013016A1 *Apr 25, 1990Oct 27, 1990Migrata Uk LtdCuvette
WO1991018656A1 *May 31, 1991Dec 12, 1991Abay SaAnalytical rotors and methods for analysis of biological fluids
WO1993008893A1 *Oct 27, 1992May 13, 1993Abay SaSample metering port for analytical rotor
WO2002060583A1 *May 18, 2001Aug 8, 2002Patrick H ClevelandMicroarrayer
Classifications
U.S. Classification494/16, 422/72, 356/39, 356/246
International ClassificationG01N21/07
Cooperative ClassificationG01N21/07
European ClassificationG01N21/07