|Publication number||US3907505 A|
|Publication date||Sep 23, 1975|
|Filing date||May 30, 1973|
|Priority date||May 30, 1973|
|Publication number||US 3907505 A, US 3907505A, US-A-3907505, US3907505 A, US3907505A|
|Inventors||Glenn Lee Beall, Fred K White|
|Original Assignee||Miles Lab|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (3), Referenced by (35), Classifications (7), Legal Events (2)|
|External Links: USPTO, USPTO Assignment, Espacenet|
United States Patent Real] et al.
[4 1 Sept. 23, 1975 SELECTIVELY DETACHABLE APPARATUS lnventors: Glenn Lee Beall, Gurnee; Fred K.
White, Glen Ellyn, both of Ill.
Miles Laboratories, Inc., Elkhart, Ind.
Filed: May 30, 1973 Appl. No.: 365,158
US. Cl. 23/259; 23/292; 220/234 Int. Cl. BOIL 3/00 Field of Search 23/292, 259; 220/234,
References Cited UNITED STATES PATENTS 5/l965 Kuhlman 220/234 X Smith ct al 220/234 Pollack et al. 220/234 X Primary Examiner-Morris O. Wolk Assistant Exuminer-Michael S. Marcus Attorney, Agent, or Firm-Louis E. Davidson  ABSTRACT Organoplastic apparatus which is capable of being frangibly separated into a plurality of individual segments. The separable segments are attached to one another by a relatively thin narrow frangible member which is integral with and extends longitudinally be tween the separable segmentspThis frangible member is reinforced by at least one integral rib member which extends transversely thereof between the separable segments.
5 Claims, 6 Drawing Figures US Patent Sept. 23,1975 Sheet 1 of2 3,907,505
f2? 29 28 E T E 7/ ;f/// 3o 2? 30 F 28 FIG. 3 FIG. 4
44 4o 39 as 260 400 H FIG. 5
SELECTIVELY DETACHABLE APPARATUS BACKGROUND ANDFRIOR ART Organoplastic laboratory apparatus having a plurality of chambers or test wells is well-known. Usually such apparatus is used in the formin which produced, and the user selects an apparatus-having the desired final number of chambers or test wells. Thelmanufacturer can make available several forms of a particular type of apparatus each form having a different numberof chambers or test wells. This increases the unit manufacturing cost and still limits the user to the particular apparatus configurations supplied by the manufacturers. a
It has been suggested in the segments each having a lesser number ofchambers than in the initial overall apparatus. It was previously suggested that the spacing between adjacent chambers could be scored,as with a glass cutter, the chambersto be separated by breaking the apparatus along the scored line. This technique is expensive from a manu facturin g standpoint. Another prior art suggestion was to form the apparatus with a thin frangible member between adjacent chambers, such member to be broken by bending. This technique had the disadvantage of requiring severalbending movements in order to break the frangible member.
There is thus a need for organoplastic apparatus which can be inexpensively manufactured and which, with a minimum of effort, can be separated as desired into segments. i
SUMMARY OF THE INVENTION In accordance with the present invention unitary organoplastic apparatus is provided which is capable of being frangibly separated into a plurality of individual segments wherein the separable segments are attached to one another by a relatively thin narrow frangible member which is integral with and extends longitudinally between such separable segments, each thin frangible member being reinforced by at least one integral rib member which extends transversely thereof be tween the separable segments.
DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view of one embodiment of the present invention;
FIG. 2 is an enlarged vertical cross-section view taken along Line 22 of FIG. 1;
FIG. 3 is an enlarged vertical cross-section view taken along Line 3-3 of FIG. 1;
FIG. 4 is a vertical cross-section view taken along Line 4-4 of FIG. 2;
FIG/ 5 is a plan view of the underside of another embodiment of the present invention; and
FIG. 6 is an enlarged partial lower perspective view of the underside shown in FIG. 5.
DESCRIPTION OF THE INVENTION Referring to FIGS. 1 and 2, the illustrated embodiment of the apparatus 10 of the present invention comprises an elongated rectangular base portion 12 having a planar top surface 14, a planar bottom surface 15 and a plurality of walls, such as 16 and 18, extending upward from said base portion to form a plurality of spaced rectangular chambers or test wells. Walls 16 prior art that a'multiplechamber apparatus be provided which is separable into form chamber 17 and walls 18 form adjacent chamber 19. In a similar fashion chambers 20, 21, 22, 23, 24 and 25 are formed on said base portion. Formed in the bottom surface 15 is alongitudinal groove 26 which separates the base portion 12 into two elongated segments 27 and 28 which are attached to each other by an elongated thin narrow member 29 which is integral with and extends longitudinally between such segments. This thin member 29 is reinforced near one end by an integral rib member 30 which extends transversely thereof between segments 27 and 28. The member 29 is reinforced near its other end with a similar integral rib member 31 which is shown in FIG. 2.
Apparatus 10 is easily and inexpensively formed as a unitary structure from frangible organoplastic materials, such as polystyrene, polyacrylate, polymethacryate, acrylonitrile-styrene copolymers, nitrile acrylonitrile-styrene copolymers, polyphenyleneoxide, phenoxy resins and the like. These materials are also desirably transparent.
The apparatus illustrated in FIG. 1 has eight chambers or test wells. If it is desired to employ only four chambers, segment27 which has the desired number of chambers can be detached from segment 28 by breaking the apparatus along thin narrow frangible member 29. when forces are applied to the apparatus, as shown by the arrows labeled F" in FIG. 4, which tend to bend the two segments 27 and 28 about a hinge formed member 29, the rib member 30 prevents such bending and, forces member 29 to conveniently break due to tensile stress. If rib member 30 were not present. member 29 wouldjust bend and would require several backand-forth bending movements before member 29 would break. The presence of rib member 30 thus enables the two segments to be separated easier than if it were not present. In view of the extended length of thin narrow frangible member 29, it is desirable to have additional rib members, such as rib member 31, disposed along its length to assist in preventing appreciable bending of member 29.
If it is desired to have the apparatus separable into still smaller segments, the structure of the embodiment shown in FIG. 5 can be employed. Elements in FIG. 5 corresponding to similar elements in FIGS. 1-4 have the same numbers with the suffix a. As shown in FIG.
5, the bottom surface 15a of base member 12a is formed with transverse grooves 32, 33, 34 and 47 as well as with longitudinal groove 26a. The transverse grooves enable the segments to be broken off along the corresponding thin narrow frangible members 35, 36, 37 and 46. Rib members 38 and 41 reinforce the frangible member 35, rib members 39 and 42 reinforce the frangible member 36, rib members and 43 reinforce the frangible member 37 and rib members 44 and 45 reinforce the frangible member 46 in the same way that rib members 3011 and 31a reinforce the frangible member 29a. Rib members 38, 39, 40, 41, 42, 43, 44 and 45 function as described above to prevent bending of the thin narrow frangible members reinforced thereby during breaking. There must be at least one rib member associated with each thin narrow frangible member that is to be broken.
Further details of the relationship between the grooves and the rib members of FIG. 5 are shown in FIG. 6. This is an enlarged partial lower perspective view of the underside shown in FIG. 5. The various elements corresponding to those of FIG. 5 are numbered identically.
The frangible member, such as element 29, should have a thickness of about twenty percent of the thickness of the adjacent members, such as element segments 27 and 28. The width of element 29, for example, between segments 27 and 28 should be about equal to its thickness. The rib member, such as rib 30, should have'a width about equal to that of the thickness of the frangible member 29 and a depth at least about equal to the width of. the rib member.
The;thin frangible member shown as element 29 in FIG. 4 has a thickness, measured in the dimension of from about 0.0l to about 0.015 in. and a width from segment 21 to segment 28 of from about 0.0] to about 0.015 in. when segments 27 and 28 have a thickness of about0.05 in. The rib members represented by element 30in FlG. 4 and element 301: in FIG. 5 have a width, measured in the dimension W", of about 0.015 in. and a depth distance, measured in the dimension Y, of from about 0.015 to about 0.045 in.
The above-described apparatus employed a total of eight chambers or test wells. It is understood that apparatus of the present invention can employ more or less than eight chambers or test wells.
The apparatus of the present invention is useful in a well-known manner in medical laboratory practice as well as in biological research to grow various cells or tissues in particular media.
What is claimed is:
1. An organoplastic device having a plurality of test wells for receiving material, thetest wells being formed on a generally planar base, the base having a top and a bottom surface, and having at least one straight groove extending edge to edge in the bottom surface and extending toward the upper surface, each said groove providing a locus for frangible separation of the formed test wells, the residue above each said groove constituting a frangible member for the said separation, each frangible member being reinforced by at least one solid rib member transverse to the respective groove within the groove and dependently contacting the upper portion of the groove, the total thickness of said rib member and said frangible member being lessthan the thickness of said generally planar base.
2. Apparatus according to claim 1 wherein the organoplastic material is selected from the class consisting of polystyrene, polyacrylate, polymethacrylate, acrylonitrile-styrene copolymers, nitrile-acrylonitrilestyrene copolymers,; polyphenyleneoxide and phenoxy resins. v
3. Apparatus according to claim 1 wherein the frangible member has a thickness of about 20 percent of the thickness of the generally planar base and a width about equal to its thickness and wherein the integral rib member has a width about equal to the thickness of the frangible member and a depth at least about equal to the width of said rib member.
4. Apparatus according to claim '1 wherein the frangible member has a thickness of from about 0.01 to about 0.015 in. and a width of from about 0.01 to about 0.015
5. Apparatus according to claim 1 wherein the reinforcing rib member has a width of about 0.015 in. and
a depth of from about 0.015 to about 0.045 in.
* l l =l
|Cited Patent||Filing date||Publication date||Applicant||Title|
|US3184054 *||Jul 13, 1962||May 18, 1965||Rca Corp||Package|
|US3520439 *||Oct 2, 1968||Jul 14, 1970||Hindmarch Smith Plastics Ltd||Container|
|US3660934 *||May 11, 1970||May 9, 1972||Vaughns Of Arizona||Molded expandable nursery tray|
|Citing Patent||Filing date||Publication date||Applicant||Title|
|US4038149 *||Dec 31, 1975||Jul 26, 1977||Linbro Scientific, Inc.||Laboratory trays with lockable covers|
|US4154795 *||Jul 21, 1977||May 15, 1979||Dynatech Holdings Limited||Microtest plates|
|US4215092 *||Jul 11, 1978||Jul 29, 1980||Osmo A. Suovaniemi||Apparatus for liquid portioning and liquid transferring|
|US4392389 *||Sep 3, 1981||Jul 12, 1983||Dragerwerk Aktiengesellschaft||Sampling tube having closing caps|
|US4472357 *||Nov 18, 1981||Sep 18, 1984||Medical Laboratory Automation, Inc.||Blood bank cuvette cassette and label therefor|
|US4495289 *||Jun 28, 1982||Jan 22, 1985||Data Packaging Corporation||Tissue culture cluster dish|
|US4829006 *||Feb 1, 1988||May 9, 1989||Difco Laboratories||Centrifugation vial and cluster tray|
|US4970053 *||Aug 18, 1989||Nov 13, 1990||Beckman Instruments, Inc.||Reagent cartridge|
|US5075082 *||Aug 10, 1990||Dec 24, 1991||Beckman Instruments, Inc.||Reagent cartridge|
|US5208161 *||Aug 25, 1988||May 4, 1993||Polyfiltronics N.A., Inc.||Filter units|
|US5470536 *||Oct 25, 1993||Nov 28, 1995||Labsystems Oy||Cuvette matrix|
|US5514343 *||Jun 22, 1994||May 7, 1996||Nunc, As||Microtitration system|
|US6096562 *||Oct 27, 1997||Aug 1, 2000||Nalge Nunc International Corporation||Multi-slide assembly including slide, frame and strip cap, and methods thereof|
|US6383820||Feb 22, 2000||May 7, 2002||Nalge Nunc International Corporation||Multi-slide assembly including slide, frame and strip cap, and methods thereof|
|US6540965||Jan 9, 2001||Apr 1, 2003||Central Labo Europe S.A.R.L.||Biological analysis and apparatus for storage of biological samples|
|US6558631||May 19, 2000||May 6, 2003||Advanced Biotechnologies Ltd.||Multi-well plates|
|US6803239||May 6, 2002||Oct 12, 2004||Nalge Nunc International Corporation||Multi-slide assembly including slide, frame and strip cap, and methods thereof|
|US6912966||Sep 18, 2003||Jul 5, 2005||E-Z Dock, Inc.||Molded plastic gangway|
|US7316805||Sep 2, 2000||Jan 8, 2008||Qiagen, Inc.||Laboratory cap and well for hanging-drop crystallization methods|
|US20050061227 *||Sep 18, 2003||Mar 24, 2005||Troy Ostreng||Molded plastic gangway|
|US20070184551 *||Apr 5, 2007||Aug 9, 2007||Jean-Pascal Viola||Crystallization Methods for laboratory cap and well|
|US20110286897 *||Nov 24, 2011||Eppendorf Ag||Microtiter plate|
|USRE34133 *||Jun 30, 1988||Nov 24, 1992||Dynatech Holdings, Ltd.||Microtest plates|
|DE9015317U1 *||Nov 7, 1990||Mar 14, 1991||Panitz, Norbert, Dr., 6500 Mainz, De||Title not available|
|DE9203583U1 *||Mar 17, 1992||May 7, 1992||Alcan Deutschland Gmbh, 3400 Goettingen, De||Title not available|
|DE10203940A1 *||Feb 1, 2002||Aug 21, 2003||Fraunhofer Ges Forschung||Plate for use in cryogenic storage of biological specimens has wells, into which specimens are placed, fits into cover in same way as drawer of matchbox, and is held in position by e.g. studs and recesses|
|DE10203940B4 *||Feb 1, 2002||Jun 14, 2006||Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.||Kryoprobenträger zur modularen Kryospeicherung|
|DE19933458B4 *||Jul 15, 1999||Aug 20, 2015||Eppendorf Ag||Einrichtungen und Systeme zum Handhaben von Flüssigkeitsproben|
|DE20101080U1 *||Jan 20, 2001||Jun 6, 2002||Evotec Biosystems Ag||Untersuchungsvorrichtung|
|DE102010008036A1 *||Feb 5, 2010||Aug 11, 2011||Eppendorf AG, 22339||Mikrotiterplatte|
|EP1053790A2 *||May 18, 2000||Nov 22, 2000||Advanced Biotechnologies Limited||Improved multi-well plates.|
|EP1348485A1 *||Feb 28, 2002||Oct 1, 2003||The Automation Partnership (Cambridge) Limited||Sample handling system and method|
|EP1754538A2||Aug 2, 2006||Feb 21, 2007||Advanced Biotechnologies Limited||Two-part microwell plates and methods of fabricating same|
|WO2000002661A1 *||Jul 13, 1999||Jan 20, 2000||Central Labo Europe (S.A.R.L.)||Plate for biological analysis and preservation of biological samples|
|WO2000029110A1 *||Nov 18, 1999||May 25, 2000||Pepscan Systems B.V.||Matrix plate, method and apparatus for sample analysis|
|U.S. Classification||422/552, 220/23.4, 422/942|
|International Classification||B01L3/00, C12M1/34|
|Jul 11, 1988||AS02||Assignment of assignor's interest|
Owner name: MILES LABORATORIES, INC., A DE CORP.
Effective date: 19880616
Owner name: NUNC, INCORPORATED, 2373 TELLER ROAD, NEWBURY PARK
|Jul 11, 1988||AS||Assignment|
Owner name: NUNC, INCORPORATED, 2373 TELLER ROAD, NEWBURY PARK
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:MILES LABORATORIES, INC., A DE CORP.;REEL/FRAME:004940/0607
Effective date: 19880616
Owner name: NUNC, INCORPORATED, A DE CORP.,CALIFORNIA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:MILES LABORATORIES, INC., A DE CORP.;REEL/FRAME:4940/607
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:MILES LABORATORIES, INC., A DE CORP.;REEL/FRAME:004940/0607