Search Images Maps Play YouTube News Gmail Drive More »
Sign in
Screen reader users: click this link for accessible mode. Accessible mode has the same essential features but works better with your reader.

Patents

  1. Advanced Patent Search
Publication numberUS3909419 A
Publication typeGrant
Publication dateSep 30, 1975
Filing dateFeb 27, 1974
Priority dateFeb 27, 1974
Publication numberUS 3909419 A, US 3909419A, US-A-3909419, US3909419 A, US3909419A
InventorsWaldemar A Ayres
Original AssigneeBecton Dickinson Co
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Plasma separator with squeezed sealant
US 3909419 A
Abstract
A blood collection and separator assembly of the type suitable for use in centrifuging blood to separate the plasma, the light phase, from the cellular portion, the heavy phase. The assembly includes a collection container and a pair of cylinders slidably disposed in the container. The first or top cylinder has a specific gravity greater than the light phase and the second or bottom cylinder has a specific gravity lighter than the heavy phase. Interposed between the cylinders are one or more layers of micro encapsulated beads of gelatin or other sealant. The average specific gravity of the first plus the second cylinders is between that of the plasma and red cells, so that the pair of cylinders float on and above the top of the heavy phase, thereby establishing the seal slightly above the interface between the light phase and heavy phase of the blood. When centrifuged the two cylinders move to the top of the packed red cells and break and squeeze out the sealant thereby forming a seal between the cylinders and the inner wall of the container slightly above the interface between the two phases of the blood.
Images(1)
Previous page
Next page
Claims  available in
Description  (OCR text may contain errors)

9 Umte States Patent 1 1 1 1 3,909,419

Ayres Sept. 30, 1975 PLASMA SEPARATOR WITH SQUEEZED [57] ABSTRACT SEALANT Inventor: Waldemar y s Rutherford A blood collection and separator assembly of the type suitable for use in centrifuging blood to separate the [73] AssigneeI Becton, Dickinson and Company, 1 plasma, the light phase, from the cellular portion, the

East Rutherford, NJ heavy phase. The assembly includes a collection container and a pair of cylinders slidably disposed in the Filedi 27, 1974 container. The first or top cylinder has a specific grav- [21] Appl. No.: 446,367 ity greater than the light phase and the second or bottom cylinder has a spec1fic gravity lighter than the 1 heavy phase. Interposed between the cylinders are one U.S-

or morg layers of micro encapsulated beads of gelatin [51] Int. Cl? BOlD 21/26 or other l t, The average specific gravity of the Field of Search 23/230 13, 258-5, 292; first plus the second cylinders is between that of the 128/2 F, 214 R, 218 M, 272; 210/83, 8 plasma and red cells, so that the pair of cylinders float 109, 131, 359, 5 A, 518, 1316- 23, 24; on and above the top of the heavy phase, thereby es- 233/1 A, 1 26 tablishing the seal slightly above the interface between the light phase and heavy phase of the blood. When l l References Cited centrifuged the two cylinders move to the top of the UNITED STATES PATENTS packed red cells and break and squeeze out the seal- 3,437,266 4/1969 Patterson .2 210/010. 23 am thereby forming a Seal between the Cylinders and 3,647,070 3/1972 Adler 210/83 h inn r ll f h n ine g ly above h in r- 3 780 935 12/1973 Lukacs et al. ZlO/DIG. 23 face between the two phases of the blood.

5 Claims, 2 Drawing Figures PLASMASEPARAT OR WITH SQUEEZED I SEALANT w I BACKGROUND OF'THE INVENTION" 1 It is known to separate blood intolits cornponlent parts by centrifugation, for example, theassembly-disclosed in- US. Pat. No; 2,460,641.- However, this particular assembly'does'not employ afmeans forsealing the separated plasma or serum phase""fror'nthe cellular phase? It is also known to provide assemblies for 'imanually separating the plasma ,orserum phase frc pr'ri th e cellular phase for example, as disclosed in U.S P at. Nos. 3,586,064; 3,661,265; 7 3,355,098; -.3,481,477; 3,512,940; a i3,693,s04. In all of thesedevices the serumiscollectd in ablood collection container and means are provided for separating the plasma 'orser'um phase from the cellular pha'se employing filters, valves, transfer tubes or. the like. I I I It is also known to provide assemblies for thesealed separation of, blood in which a piston isactuated by centrifugalforce such as is disclosed in PaLNos. 3,508,653 and 3,779,383. These devices use either a distortable piston made of resilient material or valve means associated with the piston toeffect a sealed separation after centrifugation. It is also known that a liquid sealant can be used for the separated portions of a blood sample as is coveredby US. Pat. No 3,780,935, but such patent does not disclose the two, cylinders for squeezing out the sealant of the present invention.

SUM ARY OF THEmvENTIO'N I It is an object of the present invention ito provide a plasma separator assembly including a collection container and a pair ofcylinders with encapsulated sealant interposed between the cylinders slidably disposed in the container, which cylinders havespecifi'c gravities respectively greater and lower than blood s o that upon centrifugation the sealant between the cylinders will be released and form a seal between the cylinders and the container wall slightly above the plasma-cellular interface.

It is another object of the invention to provide a plasma separator assembly which is economical to manufacture and can be used in conjunction with standard blood collecting equipment.

DESCRIPTION OF THE DRAWINGS For a better understanding of the invention, references are made to the drawings which illustrate the preferred embodiment of the invention herein.

FIG. 1 is a sectional, elevational view showing the plasma separator assembly of the present invention and also illustrating a pointed cannula penetrating one of .the stoppered ends of the container through which blood is introduced into the container prior to its separation.

FIG. 2 is a sectional, elevational view similar to the view of FIG. 1, showing the two cylinders and the interposed sealant forming an impervious barrier across the container at the plasma-cellular interface.

. IDES RIPUON OFTI-IELPREFERIRED EMBODIMENT For a better ilnderstanding of the invention herein a descriptioh of thedrawing s of the illustrative embodiishad'with particular reference to FIGS. 1 and i nula 14 so that-blood canb e transferred from a blood source'into the container under aseptic conditions'The closures 12and'13 are preferably self-sealing so that when the cannula is removed from the closure 12 there will be no loss of blood passing through 'the penetration portion of closure 12 illustrated in FIG." 1.

Disposed in the container area pair of sliding cylinde'rs, the first or top cyIin'der IS'having a specific grav it'yjgreater thanthe light phase of blood (greaterth ani 1.03) andthe second or bottom cylinder 16 having specific gravity lower than the heavy phase of blood (less than 1.09 sp.g. The average specific gravity of the first cylinder plus the second cylinder is between the plasma and red cells, for example 1.06. The fir lst cylinder carries one or more layers of rnicro encapsulated beads 17 abstaining gelatin or other sealant 18.

,When operating the separator assembly of the invention herein it is preferred that the assembly be evacuated sothat when cannula l4 penetrates closure 12 blood will fillcontainer l1 automatically. It is also contemplated to provide a separator, assemblysuitablefor use with blood collecting assembly disclosediin US. Pat, Nos. 2,460,641, 3,469,572 and 3,494,352.

After,the cannula14 is withdrawn and container 11 is filled-withbloodtheassernbly is placed ina centrifuge and the blood is separated by initially employing moderate centrifugal force. This precipitates or separates theblood cells into the lower portion of container 11 and automatically moves the cylinders .15 and 16 to float on the plasma-red cell interface. Thereafter the rotational speed of the centrifuge is increased which causes the squeeze between the cylinders to increase and ruptures the encapsulated beads and squeezes out the sealant (FIG. 2). The sealant surrounds the two cylinders and contacts the inner wall of the container and forms a seal across the container between the light and heavy phases of the blood.

Then the centrifugal forces are terminated and the separated blood sample is ready for use. As desired, the

plasma can be taken from one end and/or the concentrated red cells can be taken from the other end.

While variations of the invention herein may be had, the objectives of the invention have been illustrated and described.

Having described the invention, what is claimed is:

1. A separator assembly, capable of separating blood into its component light phase or plasma and heavy phase or cellular portion comprising:

a. a container having at least one open end which is adapted to receive blood for subsequent separation into a light phase and a heavy phase;

b. a closure sealing the open end of the container, the closure being formed of a self-sealing elastomeric material which is penetrable by a cannula through which blood to be separated is conducted into the container;

c. a pair of cylinders and rupturable capsules of sealant slidably mounted in the container, the upper cylinder having a specific gravity greater than the light phase, the lower cylinder having a specific gravity less than the heavy phase, and the combined cylinders plus capsules having a specific gravity between the two phases of blood; and

d. said rupturable capsules of sealant having a specific gravity between the two phases of blood and being interposed between the two cylinders, whereby when centrifugal force is used the blood separates into its light and heavy phases, the cylinders move through the light phase and come to a 7 stop at the top of the heavy phase, and the pressure between the cylinders ruptures the capsules and releases the sealant to form a seal slightly above the interface between the phases.

2. The separator of claim 1 wherein the said container comprises a tubular body open at each end in which closures formed of elastomeric material are mounted in sealing engagement with the tubular body and the cylinders are disposed adjacent one of said closures.

3. The separator of claim 1 wherein the sealant is gelatin.

4. A separator assembly capable of separating blood into its component light phase, plasma, and heavy phase, cellular portion comprising:

a. a container for receiving blood having closures at each end for sealing the container;

b. a pair of cylinders plus rupturable capsules of sealant slidably mounted in the container adjacent the upper closure, the upper cylinder having a specific gravity greater than the light phase, the lower cylinder having a specific gravity less than the heavy phase of blood, and the combined cylinders plus capsules having a specific gravity between the two phases of blood; and

c. said rupturable capsules of sealant having a specific gravity between the two phases of blood and being interposed between the two cylinders,

whereby when centrifugal force is used the blood separates into its light and heavy phases, the cylinders move through the light phase and come to a stop at the top of the heavy phase, and the pressure between the cylinders ruptures the capsules and releases the sealant to form a seal slightly above the interface between the phases. 5. A separator assembly capable of separating blood into its component light phase of plasma and heavy 10 phase of cellular portion comprising:

a. a tubular container having two open ends;

b. resilient closure means adapted to close said ends with at least one said closure means capable of being penetrated by a cannula for filling with blood;

c. a slidable pair of cylinders plus rupturable capsules containing sealant mounted in said container, said cylinders being located initially adjacent the closure opposite to that through which the cannula may penetrate, the space within the tube and between the penetrable closure and the cylinders forming a first chamber for receiving blood from the cannula;

d. the upper cylinder having a specific gravity greater than the light phase of blood, the lower cylinder having a specific gravity less than the heavy phase of blood, and the combined cylinders plus capsules having an average specific gravity between the two phases of blood; and

e. the rupturable capsules containing sealant having a specific gravity between the two phases of blood and being interposed between the two cylinders;

f. said slidable cylinders being adapted to move automatically to float on the top of the interface between the phases while moderate centrifugal force is used to precipitate the cellular portion of the blood with the displaced plasma flowing into the upper portion of the first chamber;

g. the cylinders being further adapted to automatically stop in the plasma slightly above the heavy phase and rupture the capsules and release the sealant to form a seal slightly above the interface between the phases.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US3437266 *Jul 3, 1967Apr 8, 1969Sondell Research & Dev CoCentrifugal separation enhancement
US3647070 *Jun 11, 1970Mar 7, 1972Technicon CorpMethod and apparatus for the provision of fluid interface barriers
US3780935 *Jul 10, 1972Dec 25, 1973Lukacs & Jacoby AssSerum separating method
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US4012325 *May 27, 1975Mar 15, 1977Eastman Kodak CompanyBiological fluid dispenser and separator
US4055501 *Jan 16, 1976Oct 25, 1977Sherwood Medical Industries Inc.Fluid collection device with phase partitioning means
US4088582 *Jan 16, 1976May 9, 1978Sherwood Medical Industries Inc.Blood phase separation means
US4169060 *Oct 25, 1977Sep 25, 1979Eastman Kodak CompanyBlood-collecting and serum-dispensing device
US4369117 *May 12, 1980Jan 18, 1983American Hospital Supply CorporationSerum separating method and apparatus
US4818386 *Oct 8, 1987Apr 4, 1989Becton, Dickinson And CompanyDevice for separating the components of a liquid sample having higher and lower specific gravities
US4877520 *Jun 6, 1988Oct 31, 1989Becton, Dickinson And CompanyDevice for separating the components of a liquid sample having higher and lower specific gravities
US4946601 *Aug 22, 1988Aug 7, 1990Sherwood Medical CompanyBlood serum separator tube
US5236604 *May 29, 1991Aug 17, 1993Sherwood Medical CompanySerum separation blood collection tube and the method of using thereof
US5269927 *Dec 16, 1991Dec 14, 1993Sherwood Medical CompanySeparation device for use in blood collection tubes
US5454958 *Sep 1, 1993Oct 3, 1995Sherwood Medical CompanyMethod for sampling in a container having a material therein which separates from a barrier material
US5736033 *Dec 13, 1995Apr 7, 1998Coleman; Charles M.Separator float for blood collection tubes with water swellable material
US6390966 *Apr 18, 2001May 21, 2002Large Scale Proteomics CorporationMethod for making density gradients
US6479298Dec 3, 1999Nov 12, 2002Becton, Dickinson And CompanyDevice and method for separating components of a fluid sample
US6905612Mar 21, 2003Jun 14, 2005Hanuman LlcPlasma concentrate apparatus and method
US7077273 *Apr 27, 2001Jul 18, 2006Harvest Technologies CorporationBlood component separator disk
US7179391May 23, 2003Feb 20, 2007Biomet Manufacturing Corp.Apparatus and method for separating and concentrating fluids containing multiple components
US7223346Apr 18, 2005May 29, 2007Hanuman LlcMethods and apparatus for isolating platelets from blood
US7374678Sep 2, 2004May 20, 2008Biomet Biologics, Inc.Apparatus and method for separating and concentrating fluids containing multiple components
US7445125May 19, 2004Nov 4, 2008Harvest Technologies CorporationMethod and apparatus for separating fluid components
US7470371Oct 19, 2006Dec 30, 2008Hanuman LlcMethods and apparatus for isolating platelets from blood
US7547272Aug 19, 2005Jun 16, 2009Harvest Technologies CorporationBlood components separator disk
US7655124Feb 2, 2010Mady AttilaApparatus to assist platelet manipulation to prevent and treat endovascular disease and its sequelae
US7694828Apr 13, 2010Biomet Manufacturing Corp.Method and apparatus for producing autologous clotting components
US7708152Jan 30, 2006May 4, 2010Hanuman LlcMethod and apparatus for preparing platelet rich plasma and concentrates thereof
US7771590Aug 10, 2010Biomet Manufacturing Corp.Method and apparatus for collecting biological materials
US7780860May 19, 2008Aug 24, 2010Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US7806276Apr 11, 2008Oct 5, 2010Hanuman, LlcBuoy suspension fractionation system
US7824559Jan 30, 2006Nov 2, 2010Hanumann, LLCApparatus and method for preparing platelet rich plasma and concentrates thereof
US7832566May 25, 2006Nov 16, 2010Biomet Biologics, LlcMethod and apparatus for separating and concentrating a component from a multi-component material including macroparticles
US7837884Dec 29, 2008Nov 23, 2010Hanuman, LlcMethods and apparatus for isolating platelets from blood
US7845499May 25, 2006Dec 7, 2010Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US7866485Jul 31, 2007Jan 11, 2011Hanuman, LlcApparatus and method for preparing platelet rich plasma and concentrates thereof
US7901584Mar 8, 2011Hanuman, LlcPlasma concentration
US7914689Mar 29, 2011Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US7922972Apr 12, 2011Harvest Technologies CorporationMethod and apparatus for separating fluid components
US7947236May 24, 2011Becton, Dickinson And CompanyDevice for separating components of a fluid sample
US7987995Aug 2, 2011Hanuman, LlcMethod and apparatus for preparing platelet rich plasma and concentrates thereof
US7992725Aug 9, 2011Biomet Biologics, LlcBuoy suspension fractionation system
US8012077May 23, 2008Sep 6, 2011Biomet Biologics, LlcBlood separating device
US8048297Nov 1, 2011Biomet Biologics, LlcMethod and apparatus for collecting biological materials
US8048320Jun 17, 2010Nov 1, 2011Biomet Manufacturing Corp.Method and apparatus for collecting biological materials
US8048321Aug 11, 2010Nov 1, 2011Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US8062534Dec 6, 2010Nov 22, 2011Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US8096422Nov 1, 2010Jan 17, 2012Hanuman LlcApparatus and method for preparing platelet rich plasma and concentrates thereof
US8105495Jan 10, 2011Jan 31, 2012Hanuman, LlcMethod for preparing platelet rich plasma and concentrates thereof
US8119013Oct 4, 2010Feb 21, 2012Hanuman, LlcMethod of separating a selected component from a multiple component material
US8133389Jul 29, 2011Mar 13, 2012Hanuman, LlcMethod and apparatus for preparing platelet rich plasma and concentrates thereof
US8163184Mar 25, 2011Apr 24, 2012Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US8187475May 29, 2012Biomet Biologics, LlcMethod and apparatus for producing autologous thrombin
US8187477May 29, 2012Hanuman, LlcMethods and apparatus for isolating platelets from blood
US8236258Aug 7, 2012Biomet Biologics, LlcMethod and apparatus for collecting biological materials
US8313954Nov 20, 2012Biomet Biologics, LlcAll-in-one means of separating blood components
US8328024Aug 4, 2011Dec 11, 2012Hanuman, LlcBuoy suspension fractionation system
US8337711Dec 25, 2012Biomet Biologics, LlcSystem and process for separating a material
US8394342Mar 12, 2013Becton, Dickinson And CompanyDensity phase separation device
US8512575Aug 6, 2012Aug 20, 2013Biomet Biologics, LlcMethod and apparatus for collecting biological materials
US8524171Apr 13, 2011Sep 3, 2013Becton, Dickinson And CompanyDevice for separating components of a fluid sample
US8551344Apr 9, 2010Oct 8, 2013Biomet Manufacturing, LlcMethod and apparatus for producing autologous clotting components
US8567609Apr 19, 2011Oct 29, 2013Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US8591391Apr 12, 2010Nov 26, 2013Biomet Biologics, LlcMethod and apparatus for separating a material
US8596470Feb 20, 2012Dec 3, 2013Hanuman, LlcBuoy fractionation system
US8603346 *Sep 22, 2011Dec 10, 2013Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US8747781Jul 21, 2009Jun 10, 2014Becton, Dickinson And CompanyDensity phase separation device
US8783470May 25, 2012Jul 22, 2014Biomet Biologics, LlcMethod and apparatus for producing autologous thrombin
US8794452Aug 1, 2013Aug 5, 2014Becton, Dickinson And CompanyDensity phase separation device
US8801586 *Dec 20, 2012Aug 12, 2014Biomet Biologics, LlcSystem and process for separating a material
US8808551Nov 15, 2010Aug 19, 2014Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US8950586Jul 1, 2013Feb 10, 2015Hanuman LlcMethods and apparatus for isolating platelets from blood
US8992862Nov 15, 2012Mar 31, 2015Biomet Biologics, LlcAll-in-one means of separating blood components
US8998000May 14, 2010Apr 7, 2015Becton, Dickinson And CompanyDensity phase separation device
US9011687Oct 7, 2013Apr 21, 2015Biomet Biologics, LlcMethod and apparatus for producing autologous clotting components
US9011800Jul 16, 2009Apr 21, 2015Biomet Biologics, LlcMethod and apparatus for separating biological materials
US9079123Aug 6, 2013Jul 14, 2015Becton, Dickinson And CompanyDensity phase separation device
US9095849Apr 11, 2013Aug 4, 2015Becton, Dickinson And CompanyDevice for separating components of a fluid sample
US9114334Dec 9, 2013Aug 25, 2015Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US9138664Dec 2, 2013Sep 22, 2015Biomet Biologics, LlcBuoy fractionation system
US9239276Oct 28, 2013Jan 19, 2016Biomet Biologics, LlcApparatus and method for separating and concentrating fluids containing multiple components
US9333445Jul 21, 2009May 10, 2016Becton, Dickinson And CompanyDensity phase separation device
US9339741May 2, 2014May 17, 2016Becton, Dickinson And CompanyDensity phase separation device
US20040166029 *Feb 24, 2004Aug 26, 2004Losada Robert J.Device for separating components of a fluid sample
US20040182788 *Mar 21, 2003Sep 23, 2004Randel DorianPlasma concentrate apparatus and method
US20040182795 *Mar 21, 2003Sep 23, 2004Randel DorianApparatus and method for concentration of plasma from whole blood
US20050186120 *Apr 18, 2005Aug 25, 2005Randel DorianMethods and apparatus for isolating platelets from blood
US20050196874 *Apr 18, 2005Sep 8, 2005Randel DorianMethods and apparatus for isolating platelets from blood
US20060032825 *Aug 19, 2005Feb 16, 2006Harvest Technologies CorporationBlood components separator disk
US20060151384 *May 19, 2004Jul 13, 2006Ellsworth James RMethod and apparatus for separating fluid components
US20070075016 *Aug 23, 2005Apr 5, 2007Biomet Manufacturing Corp.Method and apparatus for collecting biological materials
US20070208321 *May 3, 2007Sep 6, 2007Biomet Manufacturing Corp.Method And Apparatus For Collecting Biological Materials
US20080011684 *Jul 31, 2007Jan 17, 2008Dorian Randel EApparatus and method for preparing platelet rich plasma and concentrates thereof
US20090090671 *Oct 5, 2007Apr 9, 2009Mady AttilaApparatus to assist platelet manipulation to prevent and treat endovascular disease and its sequelae
US20090120852 *Nov 3, 2008May 14, 2009Ellsworth James RMethod and apparatus for separating fluid components
US20090236297 *Jun 2, 2009Sep 24, 2009Hanuman, LlcPlasma Concentrator Device
US20090283524 *May 15, 2009Nov 19, 2009Harvest Technologies CorporationBlood components separator disk
US20100160135 *Jul 21, 2009Jun 24, 2010Becton, Dickinson And CompanyDensity Phase Separation Device
US20100255977 *Jun 17, 2010Oct 7, 2010Biomet Manufacturing Corp.Method and Apparatus for Collecting Biological Materials
US20100288694 *May 14, 2010Nov 18, 2010Becton, Dickinson And CompanyDensity Phase Separation Device
US20110187021 *Aug 4, 2011Becton, Dickinson And CompanyDevice For Separating Components of a Fluid Sample
US20120015796 *Jan 19, 2012Biomet Biologics, LlcApparatus and Method for Separating and Concentrating Fluids Containing Multiple Components
US20130196425 *Dec 20, 2012Aug 1, 2013Biomet Biologics, LlcSystem and Process for Separating a Material
USRE43547Jun 15, 2011Jul 24, 2012Harvest Technologies CorporationBlood components separator disk
EP1006360A2 *Dec 6, 1999Jun 7, 2000Becton, Dickinson and CompanyDevice and method for separating components of a fluid sample
WO1996018897A1 *Dec 13, 1995Jun 20, 1996Coleman Charles MSeparator float for blood collection tubes
Classifications
U.S. Classification210/518, 422/918, 210/789
International ClassificationB01L3/14
Cooperative ClassificationB01L3/50215
European ClassificationB01L3/50215