|Publication number||US5043591 A|
|Application number||US 07/476,397|
|Publication date||Aug 27, 1991|
|Filing date||Nov 10, 1988|
|Priority date||Nov 10, 1987|
|Also published as||CA1323996C, DE3873269D1, DE3873269T2, EP0316172A1, EP0316172B1, EP0386063A1, WO1989004472A1|
|Publication number||07476397, 476397, PCT/1988/974, PCT/GB/1988/000974, PCT/GB/1988/00974, PCT/GB/88/000974, PCT/GB/88/00974, PCT/GB1988/000974, PCT/GB1988/00974, PCT/GB1988000974, PCT/GB198800974, PCT/GB88/000974, PCT/GB88/00974, PCT/GB88000974, PCT/GB8800974, US 5043591 A, US 5043591A, US-A-5043591, US5043591 A, US5043591A|
|Inventors||Ian K. Ludlow, Paul H. Kaye|
|Original Assignee||The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (3), Non-Patent Citations (3), Referenced by (41), Classifications (17), Legal Events (4)|
|External Links: USPTO, USPTO Assignment, Espacenet|
1. Field of the Invention
This invention relates to apparatus for the analysis of fluidborne particles. For examples, in the study of aerosols, aerosol dispersion and airborne particulate pollution control, there is a requirement for the rapid determination of particle size distribution especially in the diameter range 1 to 10 microns, together with some knowledge of the geometry and symmetry of individual particles. The latter information could, for example, enable particles with spherical symmetry to be identified and thus allow the counting/monitoring of liquid droplets in an environment including other solid, non-spherical particles. In the context of the present specification, the term particles is intended to apply both to solid bodies and to drops of liquid.
It is desirable for such apparatus to be able to count individual particles in a sample at rates of, typically 20,000 particles per second, to be able to distinguish between spherical and non-spherical particles in the sample and to count each type. Another desirable feature is to categorise spherical particles having diameters of a few microns into a number of size bands and also in this connection to classify particle coincidences as `non-spherical` and hence to ignore them in the compilation of size spectra based on the assumption the particle is spherical.
2. Field of Prior Art
The normal techniques for the examination of particles, as used in several instruments available commercially, employ the detection and analysis of electromagnetic radiation scattered by the particles. All such instruments use a mechanical mechanism to drive the sample air through a "sensing volume" where the carried particles are illuminated by the incident electromagnetic radiation. The radiation scattered by the particles is received by one or more detectors which convert the energy to electrical signals from which information may be extracted by appropriate electrical circuits.
Particle analysers are known, for example, as described in UK Patent Application numbers 8619050, 2044951A and U.S. Pat. No. 3,946,239. These all describe analysers which comprise a concave reflector in a scatter chamber, and a flow of sample fluid intercepted by a beam of radiation. The light scattered from individual particles in the fluid is directed by the reflector to radiation collectors and subsequently analysed. All of these, however, suffer from being cumbersome and fragile and consequently not readily portable. Moreover, light scattered at low angles from the particles in the sample is not detected by any of the above prior art systems.
Another analyser is described in UK Patent number 2041516 which discloses a particle analyser of the type described above but which has an additional feature in that the concave reflector has a transparent window in it which is used to collect back scattered light by use of lenses and photomultipliers. The intensity of the back scattered light achieved by this apparatus is very low and passes through collimation means before being collected by photomultipliers. This means that not all back scattered radiation will be detected and the apparatus required to collect the light is complex, cumbersome and relatively expensive. Moreover, the apparatus is not portable.
There is therefore a need for a particle analyser which is portable compact and relatively inexpensive and determines the size, geometry and number of particles in a sample fluid, and is additionally capable of effectively and efficiently detecting and analysing light scattered at low angles from the individual particles in the sample.
According to one aspect of the present invention a particle analyser includes a first scatter chamber, means for providing a sample of fluid in the form of a laminar flow through the first scatter chamber, a beam of radiation, adapted to intercept the sample at right angles to a direction of flow of the sample at a focal point of a first concave reflector, the first concave reflector being used to direct the radiation scattered by individual particles in the sample towards at least one radiation collector, means for converting the radiation collected into electrical signals for processing and analysis, and means for dumping the non-scattered radiation characterised in that an aperture in the first concave reflector leads to a second scatter chamber comprising a second concave reflector with a radiation collector located at its near focal point and positioned so that its far focal point is at the point of interception of the beam of radiation and the sample.
The beam of radiation may be provided by a laser which may be mounted in any one of a number of ways so that the beam intercepts the sample flow at right angles. For example, it may be mounted aligned with the principal axis of the first concave reflector; such an arrangement would make the apparatus more rugged and compact.
The first concave reflector may be a parabolic mirror, or, alternatively may be an ellipsoid mirror, which would reflect the scattered light to a single point of detection.
The advantage of having a second chamber mounted coaxially with the first chamber is so that light scattered at low angles from the individual particles in the sample can be detected and analysed also. This information is particularly useful in determining the size of particles. The second chamber also includes a second concave reflector. The second concave reflector is preferably an ellipsoid minor and has a radiation collector located at a near focal point and a far focal point at the point of interception of the beam and sample. Thus, light scattered at low angles is reflected by the ellipsoid mirror to the near focal point and collected by the radiation collector there. Radiation collectors of any suitable type may be used in the present invention and may include photomultiplier units, optical fibre leading to such units or lenses directing the radiation to a photomultiplier unit or optical fibre.
According to a second aspect of the present invention a method of particle analysis includes the steps of:
passing a sample of fluid in the form of a laminar flow through a first scatter chamber;
Passing a beam of radiation through the first scatter chamber so as to intercept the sample at right angles to a direction of flow at a focal point of a first concave reflector, the first concave reflector being used to direct the radiation towards at least one radiation collector; characterised in that the back scattered radiation is collected in a second chamber, including a second concave reflector and leading from an aperture in the first concave reflector, by use of a radiation collector located at a near focal point of the second concave reflector which is also positioned so that its far focal point is at the point of interception of the beam of radiation and the sample; converting the radiation collected into electrical signals, processing and analysing the electrical signals; and dumping the non-scattered radiation.
The sample may be an aerosol.
The number of embodiments of the invention will now be described by way of example only, with reference to the accompanying drawings, in which:
FIG. 1 is a side view in section of a preferred embodiment of the invention;
FIG. 1a is a view along the line in FIG. 1;
FIG. 2 is a side view in section of another embodiment of the invention;
As shown in FIG. 1a first scattering chamber 10 includes a first concave reflector in the form of a parabolic mirror 11, lenses 12, and radiation collectors 13. A laser 14 is mounted aligned with the principal axis of the parabolic mirror 11 and directs a beam 15 of radiation towards the focal point 16 of the parabolic mirror 11 where it intercepts with the sample fluid 17 in the form of a laminar flow. An aperture 18 leads to a second chamber 19 which includes a second concave reflector in the form of an ellipsoid reflector 20 and a radiation collector 21 located at the near focal point of the ellipsoid reflector 20 and the ellipsoid reflector is positioned so that its far focal point is situated at the focal point 16 of the first parabolic reflector 11. A beam dump 22, typically a Raleigh horn is located at an aperture in the ellipsoid mirror 20 to collect the non-scattered radiation. Radiation collectors 13 and 21 are connected to photomultiplier tubes 23. FIG. 1a shows a possible arrangement of radiation collectors 13 around the laser 14. Although only three collectors are shown here, any number of detectors may be located radially around the laser 14.
Another embodiment of the invention is shown in FIG. 2. In this embodiment both a first concave reflector 50 and a second concave reflector 51 are ellipsoid mirrors. Once again, the laser 14 is at an angle of 90° to the principal axes of the reflectors, so that mirror 41 directs the beam 15 along the principal axis. The sample 17 is directed at right angles to the laser beam 15 and intercepts it at the near focal point 16 of the first ellipsoid reflector 50. The second ellipsoid reflector 51 is positioned so that its far focal point coincides with point 16. Photomultiplier tubes 23 are located at the far focal point of the first concave reflector 50 and at the near focal point of the second concave reflector 51 to collect the scattered radiation. The beam dump 22 is located within the second scatter chamber 19 to dump the non-scattered radiation. The radiation collector 23 in FIG. 2 is positioned to face the aperture 18 in the first chamber 10 as opposed to being placed at 90° to this direction as shown in FIG. 1. The latter arrangement would collect relatively more radiation of low angle deflection, but less overall since only deflections in the direction of the face of the collector will be recorded.
In use, the sample of fluid 17 is supplied in laminar flow by means of a sheath of constant velocity air being supplied around the sample, as shown in FIGS. 1 and 2. This is so that the outer parts of the sample flow have the same velocity as the inner parts. The outer parts of the sample would otherwise flow more slowly due to friction with stationary air next to the sample flow. Additionally, a coaxial tube supplying the sheath of air is designed to dynamically focus particles in the sample to provide a laminar flow of particles. The laser beam 15 intercepts at right angles the flow of fluid 17 and light is scattered from the individual particles contained in the fluid. The scattered radiation reflects off the walls of the first concave reflector in the first scatter chamber 10. If first concave reflector is a parabolic mirror (FIG. 1) the radiation is reflected parallel to its principal axis or if it is an ellipsoid mirror 50 (in FIG. 2), the radiation is directed to the far focal point of the mirror. This deflected radiation is then directed towards photomultiplier tubes 23 either directly, as in FIG. 2, or by using lenses 12 as in FIG. 1 to direct the radiation towards the photomultiplier units 23.
Radiation scattered at low angles by the particles is collected in the second chamber 19, which may include an ellipsoid mirror 20 and 51 in FIGS. 1 and 2 and radiation collectors which may be a photomultiplier tube 23 as in FIG. 2 or a lens 21 in FIG. 1 leading to such a tube 23.
All the radiation collected is then converted into electrical signals, processed and analysed, and the information may be extracted by appropriate electronic circuits.
Although this invention has been described by way of example and with reference to possible embodiments thereof, it is to be understood that modifications or improvements may be made without departing from the scope of the invention as defined in the appended claims.
|Cited Patent||Filing date||Publication date||Applicant||Title|
|US4200802 *||Mar 28, 1979||Apr 29, 1980||The United States Of America As Represented By The United States Department Of Energy||Parabolic cell analyzer|
|FR2535051A1 *||Title not available|
|GB2041516A *||Title not available|
|1||Aerosol Measurement, editor D. Lundgren, published 1979 Gainerville, (US) R. W. Storey: "Aerosol Field Measurements Using Light-Scattering Photometers", pp. 241-259, see pp. 241-244: Instruments.|
|2||*||Aerosol Measurement, editor D. Lundgren, published 1979 Gainerville, (US) R. W. Storey: Aerosol Field Measurements Using Light Scattering Photometers , pp. 241 259, see pp. 241 244: Instruments.|
|3||*||International Search Report.|
|Citing Patent||Filing date||Publication date||Applicant||Title|
|US5467189 *||Jan 12, 1995||Nov 14, 1995||Venturedyne, Ltd.||Improved particle sensor and method for assaying a particle|
|US5471299 *||Feb 18, 1993||Nov 28, 1995||The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland||Apparatus and method for the analysis of particle characteristics using monotonically scattered light|
|US5767967 *||Jan 2, 1997||Jun 16, 1998||Yufa; Aleksandr L.||Method and device for precise counting and measuring the particulates and small bodies|
|US7631568||Aug 28, 2007||Dec 15, 2009||Quest Technologies||Particulate monitor|
|US7713687||Nov 29, 2001||May 11, 2010||Xy, Inc.||System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations|
|US7723116||May 25, 2006||May 25, 2010||Xy, Inc.||Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm|
|US7758811||Mar 29, 2004||Jul 20, 2010||Inguran, Llc||System for analyzing particles using multiple flow cytometry units|
|US7771921||Sep 28, 2006||Aug 10, 2010||Xy, Llc||Separation systems of frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations|
|US7799569||Mar 16, 2009||Sep 21, 2010||Inguran, Llc||Process for evaluating staining conditions of cells for sorting|
|US7820425||Dec 7, 2006||Oct 26, 2010||Xy, Llc||Method of cryopreserving selected sperm cells|
|US7833147||Jul 22, 2005||Nov 16, 2010||Inguran, LLC.||Process for enriching a population of sperm cells|
|US7838210||Mar 29, 2005||Nov 23, 2010||Inguran, LLC.||Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations|
|US7855078||Aug 15, 2003||Dec 21, 2010||Xy, Llc||High resolution flow cytometer|
|US7892725||Mar 29, 2005||Feb 22, 2011||Inguran, Llc||Process for storing a sperm dispersion|
|US7929137||Sep 8, 2009||Apr 19, 2011||Xy, Llc||Optical apparatus|
|US7943384||Jun 7, 2010||May 17, 2011||Inguran Llc||Apparatus and methods for sorting particles|
|US7987695||Sep 9, 2009||Aug 2, 2011||3M Innovative Properties Company||Particulate monitor|
|US8137967||Aug 21, 2006||Mar 20, 2012||Xy, Llc||In-vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations|
|US8211629||Aug 1, 2003||Jul 3, 2012||Xy, Llc||Low pressure sperm cell separation system|
|US8427640||Oct 21, 2009||Apr 23, 2013||M-I L.L.C.||Method and apparatus for measuring particle size distribution in drilling fluid|
|US8486618||Jul 18, 2011||Jul 16, 2013||Xy, Llc||Heterogeneous inseminate system|
|US8497063||Aug 24, 2010||Jul 30, 2013||Xy, Llc||Sex selected equine embryo production system|
|US8652769||Aug 9, 2010||Feb 18, 2014||Xy, Llc||Methods for separating frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations|
|US8664006||Mar 1, 2013||Mar 4, 2014||Inguran, Llc||Flow cytometer apparatus and method|
|US8709817||Feb 7, 2013||Apr 29, 2014||Inguran, Llc||Systems and methods for sorting particles|
|US8709825||Mar 1, 2013||Apr 29, 2014||Inguran, Llc||Flow cytometer method and apparatus|
|US8748183||Feb 7, 2013||Jun 10, 2014||Inguran, Llc||Method and apparatus for calibrating a flow cytometer|
|US8813583||Jun 21, 2011||Aug 26, 2014||3M Innovative Properties Company||Particulate monitor|
|US9007580||Apr 22, 2013||Apr 14, 2015||Schlumberger Norge As||Method and apparatus for measuring particle size distribution in drilling fluid|
|US9040304||Mar 12, 2014||May 26, 2015||Inguran, Llc||Multi-channel system and methods for sorting particles|
|US9365822||Feb 11, 2013||Jun 14, 2016||Xy, Llc||System and method for sorting cells|
|US9377390||Apr 10, 2015||Jun 28, 2016||Inguran, Llc||Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm|
|US9422523||Feb 11, 2013||Aug 23, 2016||Xy, Llc||System and method for sorting cells|
|US20090056417 *||Aug 28, 2007||Mar 5, 2009||Quest Technologies, Inc.||Particulate monitor|
|US20090320562 *||Sep 9, 2009||Dec 31, 2009||Kilps Patrick J||Particulate monitor|
|US20100315635 *||Jul 4, 2008||Dec 16, 2010||Christoph Janzen||Device and method for measuring static and dynamic scattered light in small volumes|
|US20110192595 *||Oct 21, 2009||Aug 11, 2011||M-I L.L.C.||Method and apparatus for measuring particle size distribution in drilling fluid|
|WO1995009354A1 *||Dec 6, 1993||Apr 6, 1995||Venturedyne Limited||Improved particle sensor and method for assaying a particle|
|WO2010048276A2 *||Oct 21, 2009||Apr 29, 2010||M-I L.L.C.||Method and apparatus for measuring particle size distribution in drilling fluid|
|WO2010048276A3 *||Oct 21, 2009||Jul 29, 2010||M-I L.L.C.||Method and apparatus for measuring particle size distribution in drilling fluid|
|WO2010058102A1||Nov 17, 2009||May 27, 2010||Centre National De La Recherche Scientifique - Cnrs -||Method and system for analysing solid particles in a medium|
|U.S. Classification||250/574, 356/343, 250/575|
|International Classification||G01N21/53, G01N15/00, G01N15/02, G01N15/14, G01N|
|Cooperative Classification||G01N2015/0294, G01N2015/1413, G01N2015/1493, G01N2021/6469, G01N15/1434, G01N15/1431, G01N2015/0238|
|European Classification||G01N15/14F, G01N15/14E2|
|Jun 4, 1990||AS||Assignment|
Owner name: SECRETARY OF STATE FOR DEFENCE IN HER BRITANNIC MA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:LUDLOW, IAN K.;KAYE, PAUL H.;REEL/FRAME:005332/0914
Effective date: 19900409
|Jan 10, 1995||FPAY||Fee payment|
Year of fee payment: 4
|Jan 19, 1999||FPAY||Fee payment|
Year of fee payment: 8
|Jan 17, 2003||FPAY||Fee payment|
Year of fee payment: 12