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Publication numberUS5096672 A
Publication typeGrant
Application numberUS 07/573,377
Publication dateMar 17, 1992
Filing dateAug 24, 1990
Priority dateAug 28, 1989
Fee statusPaid
Also published asDE69021672D1, DE69021672T2, DE69032860D1, DE69032860T2, EP0415307A2, EP0415307A3, EP0415307B1, EP0649679A2, EP0649679A3, EP0649679B1
Publication number07573377, 573377, US 5096672 A, US 5096672A, US-A-5096672, US5096672 A, US5096672A
InventorsJukka Tervamaki, Kari Vauramo
Original AssigneeLabsystems Oy
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Cuvette matrix and its tray
US 5096672 A
The invention concerns a cuvette matrix and its tray. The matrix comprises adjacent cuvettes (2) connected with one another by flexible connecting elements (3). The tray has an aperture (8) for each well, with a flexible clamping element (9).
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We claim:
1. A cuvette matrix and tray therefor comprising at least one row of cuvettes flexibly interconnected one to the other by stems to allow for flexible play between each cuvette,
each of said cuvettes being in the shape of a well of substantially cylindrical shape,
each of said wells having an open top and a closed bottom,
the cylindrical walls of each well being thickened at the upper portion thereof and slightly tapered at its lower portion to enable reception of said cuvette matrix by said tray,
said tray for receiving said cuvette matrix comprising a frame having by rows of cuvette-receiving apertures having side walls adapted to frictionally receive and support each of said flexibly interconnected wells when said interconnected wells are inserted tapered end first into corresponding apertures of said tray,
said apertures being defined by substantially rigidly disposed side walls defining said apertures,
the side walls intersection to form each of said apertures,
an inward wall of each of said apertures being severed to provide at least one flexibly deformable clamping element in each of said apertures for frictionally engaging and holding each of said wells.
2. The cuvette matrix and tray as in claim 1, wherein a peripheral shoulder is provided on each of said wells between the thickened upper portion and said lower tapered portion, and wherein stop means is provided in each of said apertures to engage said peripheral shoulder.
3. The cuvette matrix and tray of claim 2, wherein the apertures of said tray are quadrangular in shape.
4. The cuvette matrix and tray as in claim 3, wherein one or more interconnected wells can be removed from the matrix at a time.
5. The cuvette matrix and tray as in claim 4, wherein said connecting stems are breakable such that a well si removable by breaking the stem connected to said well.
6. The cuvette matrix and tray as in claim 5, wherein the breakable stems flexibly interconnecting one well to the other are connectingly disposed along the sides of the wells, or along alternate sides thereof.
7. The cuvette matrix and tray as in claim 3, wherein said severed inward wall of each of said apertures is configurated as a deformable flexible finger for frictionally holding the confined well against an opposite wall of said aperture.
8. The cuvette matrix and tray as in claim 7, wherein said flexible finger is adapted to be horizontally flexible.
9. The cuvette matrix and tray as in claim 8, wherein said aperture has only one finger for holding said well therein.
10. The cuvette matrix and tray as in claim 1, wherein the closed bottom of each of said well is optically light transmitting.
11. The cuvette matrix and tray as in claim 1, wherein said inward wall of each of said apertures in said tray is severed diagonally at substantially the center of said side wall to provide a diagonal gap in said inward wall and thereby form a pair of fingers which flex horizontally when a cuvette is frictionally forced into said aperture.

This invention concerns a cuvette matrix comprising of rows of flexibly connected cuvette or wells and a tray therefor. If necessary, smaller parts of the matrix can be removed and put back into the tray. The cuvette matrix is especially suitable for use in different diagnostic measurements, for example EIA-assays. Cuvette matrices can, for example, form a so called microtitration plate.

Generally used for diagnostic assays are test plates formed by rows of cuvettes, for example, the so called microtitration plate into the cuvettes of which the samples are placed. Generally speaking a standard plate is used comprising 8>12 cuvettes with a distribution of 9 mm. Also known are cuvette sets in which a smaller part or segment of which can be removed, if necessary. Thus, it is not necessary to use the whole set, especially where there are only a few samples to measure.

U.S. Pat. No. 4,154,795 discloses a micro-titration plate the wells of which are connected to one another by rigid, straight stems. The stems can be broken and in this way it is possible to remove a predetermined amount of wells from the plate. The tray of the plate is equipped with posts placed in spaces between the wells. One problem with this solution is the fact that the wells do not stand upright in the tray properly, when the tray is moved. For example during stages of washing, it is often necessary to turn the tray upside down, whereby the wells tend to fall out. Also the fact that the different wells are at different heights in the tray can cause difficulties with the measuring device.


The cuvette matrix in accordance with the present invention and its tray with some of its favorable applications is disclosed in the claims.

An important aspect of the invention is the fact that the cuvettes are connected with one another with flexible connecting elements and the fact that there are flexible clamping elements in the tray to hold the cuvettes in place with help of the friction.


In drawings of the detailed description of the invention FIGS. 1a and 1b show one row-formed cuvette matrix in accordance with the invention as viewed from side and above, and FIGS. 2a and 2b show one cuvette matrix tray in accordance with the invention as viewed from side and above; and FIGS. 3a and 3b show a detail of the tray in accordance with FIGS. 2a and 2b, where the matrix in accordance with FIGS. 1a and 1b and one of its cuvettes has been placed in as viewed from side and above.


The cuvette matrix in accordance with the invention is formed by straight rows of cuvettes, with one of them or several side by side. The matrix is advantageously made of some suitable plastic material by injection-moulding. The cuvettes are preferably cylindric cups or wells. For optical measurements, if necessary, their bottom is transparent. The matrices are suitable for use especially in different diagnostic assays on fluid samples, such as in EIA assays. If necessary, the cuvettes can be pretreated, for example the content can be coated with antigen of the antibody to be assayed.

At least a part of adjacent cuvettes of the matrix connected with one another by flexible connecting elements, that permit the cuvettes to move a little or provide play in relation to one another, at least horizontally. The connecting elements are situated in a way that the lower part of each cuvette can be put in the aperture of the tray described later. The connecting elements are preferably like stems and they are placed to connect the cuvettes at their top part. The required flexibility is attained by placing the stems at a distance from the centre line of the cuvette row. It is also possible to use curved stems right by the centre line.

The cuvettes are preferably connected to one another in a way that a desired amount of cuvettes can readily be removed from the matrix. The removability is preferably attained by employing connecting elements which are readily breakable.

The tray is formed by a frame with an aperture at least for one cuvette of the matrix. The aperture includes a flexible clamping element that fastens the cuvette into the aperture with help of the friction, preferably by pressing its lower part from the sides. The clamping element, anyway, provides sufficient play so that the cuvette can be pushed into the aperture. The clamping element may press the cuvette from one side or several sides. According to one embodiment the clamping element presses the cuvette against a rigid frame. The clamping element may comprise one or more flexible fingers. The finger is preferably flexible horizontally.

FIGS. 1-4 illustrate one application of the invention adapted to a micro test plate comprising apertures 8×12.

FIGS. 1a and 1b illustrate a one-row cuvette matrix 1. The single cuvettes, i.e. wells 2 thereof are preferably connected to one another by narrow stems 3. The stems 3 are fixed near the top part of the wells. The stems 3 are placed at a distance from the centre line of the cuvette row at sides of the cuvette row, so that the stems 3 next to each other are each in opposite sides. The stems 3 provide sufficient play so that each distance between adjacent wells 2 can get smaller and wider for some hundredths or tenths of a millimeter.

The inside of the wells 2 is cylindrical. Their bottom forms a light transmission measuring window. The window is protected against scratching with a collar around the window.

On the outer surface of the wells 2, slightly below the middle there is a shoulder 5, broader than the lower part of the well, which determine how deep the well can be pushed into the tray 6. The outer surface of the lower part of the well 2 is cone-shaped, tapered slightly downwards.

The stems 3 can be broken by hand. This enables the required amount of wells 2 to be readily removed.

In the both ends of the cuvette matrix there are flanges 7 at the top part, that can also be broken off.

In the tray 6 in accordance with FIGS. 2a and 2b there are 8×12 apertures 8, in cross-sectional quadrangle shape. The side of the aperture 8 is slightly shorter than the biggest diameter of the lower part of the well 2. The apertures 8 form 8 rows, marked with letters (A-H) and 12 columns, marked with numbers (1-12) that is, 8×12. The apertures 8 are delimited by a rectangular frame with separation walls perpendicular to one another.

Separation walls parallel with the columns are integral and rigid. From the second separation wall on, from the side, every other wall parallel with the rows is also integral and rigid, the separation walls extending laterally and longitudinally across said frame.

From the first separation wall, from the side, each inward wall parallel with the rows is cut off or severed vertically at the centre line of the column, but diagonally against the separation wall and so that there is a small gap between the cut-off ends. The thus formed fingers 9, parallel with the separation walls of the rows, are slightly bent in horizontal direction. Thus, a well 2 can be pushed into each aperture 8, whereby the finger 9 bends away from the centre of the aperture. The finger 9 helps to keep the well 2 in the aperture with the aid of the friction.

The upper edge of the rigid separation walls 10 provides a stop for shoulder 5 on the outer surface of the well 2. Also on the sides of the tray the frame has ancons against the shoulders 5.

On the side of the tray there is a pin 11 at the lower end of each column. One head flange 7 of the cuvette matrix has a corresponding hole 12. Thus, the cuvette matrix is capable of being positioned the right way on the tray.

Lower edges of the tray extend lower than the bottoms of the wells 2 in the tray. Additionally, there are lips in the corners 13 of the tray to enable the trays to be readily piled.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US3175695 *Jun 15, 1961Mar 30, 1965Advance Scient CorpTissue culture tube rack means
US3470851 *Nov 21, 1967Oct 7, 1969Cannon Robert WIncubator egg tray
US3713985 *Oct 19, 1970Jan 30, 1973Kantor FDevice and method for testing potency of biological control reagents
US4154795 *Jul 21, 1977May 15, 1979Dynatech Holdings LimitedMicrotest plates
US4472357 *Nov 18, 1981Sep 18, 1984Medical Laboratory Automation, Inc.Blood bank cuvette cassette and label therefor
BE902982A2 * Title not available
EP0035779A1 *Mar 6, 1981Sep 16, 1981Walter Graf u. Co. GmbH & Co.Removable base for a cylindrical recipient
EP0106662B1 *Oct 12, 1983Nov 23, 1988Dynatech Laboratories, IncorporatedNon-fluorescent vessels for holding test samples in fluorescent assays
EP0114056A2 *Jan 10, 1984Jul 25, 1984Hoechst AktiengesellschaftSample stand
EP0188009A1 *Dec 31, 1985Jul 23, 1986BEHRINGWERKE AktiengesellschaftArrangement of cuvettes
EP0365827A2 *Sep 21, 1989May 2, 1990LABORATORIUM PROF. DR. RUDOLF BERTHOLD GmbH & Co. KGDevice for holding a plurality of sample containers for carrying out radiation measurements
FR2601452A1 * Title not available
GB2064998A * Title not available
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US5250440 *Jul 16, 1992Oct 5, 1993Schiapparelli Biosystems, Inc.Cuvette delivery module and turntable for a chemical analyzer
US5308584 *Sep 9, 1992May 3, 1994Biohit OyCuvette matrix tray
US5460783 *Jun 14, 1994Oct 24, 1995Synbiotics CorporationApparatus for automatically removing microtiter well-strips from well-strip holders
US5470536 *Oct 25, 1993Nov 28, 1995Labsystems OyCuvette matrix
US5508005 *Oct 26, 1993Apr 16, 1996Costar CorporationNon-screeching laboratory article
US5514343 *Jun 22, 1994May 7, 1996Nunc, AsMicrotitration system
US5576214 *Sep 14, 1994Nov 19, 1996Johnson & Johnson Clinical Diagnostics, Inc.Method of supplying disposable tips to an aspirator
US5650125 *Oct 14, 1993Jul 22, 1997Bosanquet; Andrew GeorgeMethod and apparatus for conducting tests
US5948363 *Apr 22, 1996Sep 7, 1999Gaillard; PatrickMicro-well strip with print tabs
US6106784 *Sep 26, 1997Aug 22, 2000Applied Chemical & Engineering Systems, Inc.Thawing station
US6171554Oct 2, 1996Jan 9, 2001Matrix Technologies CorporationApparatus and method for alphanumerically identifying and arranging test tubes
US6193064 *Nov 4, 1998Feb 27, 2001J. G. Finneran Associates, Inc.Multi-tier vial plate
US6221027 *Dec 2, 1999Apr 24, 2001Isadore PiteskyModular allergy testing apparatus
US6258041 *Dec 6, 1999Jul 10, 2001Isadore PiteskyStrip tray allergy testing apparatus
US6436351 *Jul 15, 1998Aug 20, 2002Deltagen Research Laboratories, L.L.C.Microtitre chemical reaction system
US6528302Jan 22, 2002Mar 4, 2003M.J. Research, Inc.Thin-well microplate and methods of making same
US6540965 *Jan 9, 2001Apr 1, 2003Central Labo Europe S.A.R.L.Biological analysis and apparatus for storage of biological samples
US6558631May 19, 2000May 6, 2003Advanced Biotechnologies Ltd.Multi-well plates
US6558947Aug 22, 2000May 6, 2003Applied Chemical & Engineering Systems, Inc.Thermal cycler
US6896144 *Jun 25, 2002May 24, 2005Innovative MicroplateFiltration and separation apparatus and method of assembly
US6918738Mar 11, 2002Jul 19, 2005Diversa CorporationStackable sample holding plate with robot removable lid
US7347977 *Jul 20, 2004Mar 25, 2008Eppendorf AgMicrotitration plate
US7402281Jul 18, 2003Jul 22, 2008Siemens Healthcare Diagnostics Inc.Magazine for inventorying reaction cuvettes in an automatic analyzer
US7631765Dec 6, 2007Dec 15, 2009Lincoln Diagnostics, Inc.Skin testing-device system
US7632465Dec 3, 2004Dec 15, 2009Thermo Fisher Scientific OyVessel tray
US7892504 *Jun 22, 2006Feb 22, 2011Tsubakimoto Chain Co.Pharmaceutical sample storage system
US7922672 *Jun 8, 2006Apr 12, 2011Lincoln Diagnostics, Inc.Skin testing-device system
US8197776 *May 27, 2009Jun 12, 2012Thermo Fisher Scientific OyReaction vessel and method for the handling thereof
US8591791Nov 7, 2007Nov 26, 2013Eppendorf AgMethod of manufacturing a microtitration plate
US8641968May 27, 2009Feb 4, 2014Thermo Fisher Scientific OyChemical analyser, method for sample-based analysis, device for handling cuvettes, and loading method
US8968679 *May 19, 2005Mar 3, 2015Emd Millipore CorporationReceiver plate with multiple cross-sections
US9138742Oct 15, 2014Sep 22, 2015Emd Millipore CorporationReceiver plate with multiple cross-sections
US20020009397 *Jun 6, 2001Jan 24, 2002Taisuke HironoCuvette stand and stand with cuvettes
US20020076356 *Nov 15, 2001Jun 20, 2002Thomas RothmannApparatus for receiving samples
US20020176807 *Jul 10, 2002Nov 28, 2002Combichem, Inc.Microtitre chemical reaction system
US20020195386 *Jun 25, 2002Dec 26, 2002Young Stephen G.Filtration and separation apparatus and method of assembly
US20030054543 *Nov 1, 2002Mar 20, 2003Lafferty William MichaelDevice for moving a selected station of a holding plate to a predetermined location for interaction with a probe
US20050013747 *Jul 18, 2003Jan 20, 2005Thai Huynh-BaMagazine for inventorying reaction cuvettes in an automatic analyzer
US20050019225 *Aug 16, 2004Jan 27, 2005Sanadi Ashok RameshMethod and apparatus for preventing cross-contamination of multi-well test plates
US20050058578 *Jul 20, 2004Mar 17, 2005Eppendorf AgMicrotitration plate
US20060263875 *May 19, 2005Nov 23, 2006Scott Christopher AReceiver plate with multiple cross-sections
US20060269641 *May 12, 2006Nov 30, 2006Applera CorporationThermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US20070017885 *Jun 22, 2006Jan 25, 2007Hiroyuki TaikeSample storage system for pharmaceutical development
US20070031296 *Aug 1, 2006Feb 8, 2007Advanced Biotechnologies LimitedImproved two-part microwell plates and methods of fabricating same
US20070217958 *Dec 3, 2004Sep 20, 2007Tapani OrhaVessel Tray
US20070299361 *Jun 8, 2006Dec 27, 2007Lincoln Diagnostics, Inc.Skin testing-device system
US20080073242 *Dec 6, 2007Mar 27, 2008Lincoln Diagnostics, Inc.Skin Testing-Device System
US20080084004 *Nov 7, 2007Apr 10, 2008Eppendorf AgMicrotitration Plate
US20080293157 *May 24, 2007Nov 27, 2008Gerald FredericksonApparatus and method of performing high-throughput cell-culture studies on biomaterials
US20110064543 *May 27, 2009Mar 17, 2011Thermo Fisher Scientific OyReaction vessel and method for the handling thereof
US20110091986 *May 27, 2009Apr 21, 2011Thermo Fisher Scientific OyChemical analyser, method for sample-based analysis, device for handling cuvettes, and loading method
US20110123415 *Jul 15, 2009May 26, 2011Bruce PetersonMicroplate and methods for making the same
US20130237443 *Mar 6, 2013Sep 12, 2013Scienion AgSpotting plate and process for its production
US20150108076 *Oct 21, 2014Apr 23, 2015Health Diagnostic Laboratory, Inc.Test tube rack insert device
CN103648647A *Jun 22, 2012Mar 19, 2014霍尔格·彼尼克Cuvette module having an electrically conductive cuvette carrier
CN103648647B *Jun 22, 2012Apr 1, 2015霍尔格·彼尼克Cuvette module having an electrically conductive cuvette carrier
DE10203940A1 *Feb 1, 2002Aug 21, 2003Fraunhofer Ges ForschungPlate for use in cryogenic storage of biological specimens has wells, into which specimens are placed, fits into cover in same way as drawer of matchbox, and is held in position by e.g. studs and recesses
DE10203940B4 *Feb 1, 2002Jun 14, 2006Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.Kryoprobenträger zur modularen Kryospeicherung
DE10262208B4 *Feb 1, 2002Apr 3, 2008Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.Probenträger für eine Vielzahl von Kryoproben
U.S. Classification422/552, 422/942, 220/23.4, 206/558, 220/23.86, 206/560, 220/23.83, 422/560
International ClassificationB01L3/00, G01N35/02, G01N1/10, G01N33/48, B01L9/06, G01N33/53, G01N33/543
Cooperative ClassificationB01L3/5085, B01L3/50855, B01L9/06
European ClassificationB01L3/50855, B01L3/5085, B01L9/06
Legal Events
Aug 24, 1990ASAssignment
Effective date: 19900321
Jun 1, 1993CCCertificate of correction
Aug 31, 1995FPAYFee payment
Year of fee payment: 4
Aug 16, 1999FPAYFee payment
Year of fee payment: 8
Sep 3, 2003FPAYFee payment
Year of fee payment: 12