|Publication number||US5096672 A|
|Application number||US 07/573,377|
|Publication date||Mar 17, 1992|
|Filing date||Aug 24, 1990|
|Priority date||Aug 28, 1989|
|Also published as||DE69021672D1, DE69021672T2, DE69032860D1, DE69032860T2, EP0415307A2, EP0415307A3, EP0415307B1, EP0649679A2, EP0649679A3, EP0649679B1|
|Publication number||07573377, 573377, US 5096672 A, US 5096672A, US-A-5096672, US5096672 A, US5096672A|
|Inventors||Jukka Tervamaki, Kari Vauramo|
|Original Assignee||Labsystems Oy|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (13), Referenced by (59), Classifications (21), Legal Events (5)|
|External Links: USPTO, USPTO Assignment, Espacenet|
This invention concerns a cuvette matrix comprising of rows of flexibly connected cuvette or wells and a tray therefor. If necessary, smaller parts of the matrix can be removed and put back into the tray. The cuvette matrix is especially suitable for use in different diagnostic measurements, for example EIA-assays. Cuvette matrices can, for example, form a so called microtitration plate.
Generally used for diagnostic assays are test plates formed by rows of cuvettes, for example, the so called microtitration plate into the cuvettes of which the samples are placed. Generally speaking a standard plate is used comprising 8>12 cuvettes with a distribution of 9 mm. Also known are cuvette sets in which a smaller part or segment of which can be removed, if necessary. Thus, it is not necessary to use the whole set, especially where there are only a few samples to measure.
U.S. Pat. No. 4,154,795 discloses a micro-titration plate the wells of which are connected to one another by rigid, straight stems. The stems can be broken and in this way it is possible to remove a predetermined amount of wells from the plate. The tray of the plate is equipped with posts placed in spaces between the wells. One problem with this solution is the fact that the wells do not stand upright in the tray properly, when the tray is moved. For example during stages of washing, it is often necessary to turn the tray upside down, whereby the wells tend to fall out. Also the fact that the different wells are at different heights in the tray can cause difficulties with the measuring device.
The cuvette matrix in accordance with the present invention and its tray with some of its favorable applications is disclosed in the claims.
An important aspect of the invention is the fact that the cuvettes are connected with one another with flexible connecting elements and the fact that there are flexible clamping elements in the tray to hold the cuvettes in place with help of the friction.
In drawings of the detailed description of the invention FIGS. 1a and 1b show one row-formed cuvette matrix in accordance with the invention as viewed from side and above, and FIGS. 2a and 2b show one cuvette matrix tray in accordance with the invention as viewed from side and above; and FIGS. 3a and 3b show a detail of the tray in accordance with FIGS. 2a and 2b, where the matrix in accordance with FIGS. 1a and 1b and one of its cuvettes has been placed in as viewed from side and above.
The cuvette matrix in accordance with the invention is formed by straight rows of cuvettes, with one of them or several side by side. The matrix is advantageously made of some suitable plastic material by injection-moulding. The cuvettes are preferably cylindric cups or wells. For optical measurements, if necessary, their bottom is transparent. The matrices are suitable for use especially in different diagnostic assays on fluid samples, such as in EIA assays. If necessary, the cuvettes can be pretreated, for example the content can be coated with antigen of the antibody to be assayed.
At least a part of adjacent cuvettes of the matrix connected with one another by flexible connecting elements, that permit the cuvettes to move a little or provide play in relation to one another, at least horizontally. The connecting elements are situated in a way that the lower part of each cuvette can be put in the aperture of the tray described later. The connecting elements are preferably like stems and they are placed to connect the cuvettes at their top part. The required flexibility is attained by placing the stems at a distance from the centre line of the cuvette row. It is also possible to use curved stems right by the centre line.
The cuvettes are preferably connected to one another in a way that a desired amount of cuvettes can readily be removed from the matrix. The removability is preferably attained by employing connecting elements which are readily breakable.
The tray is formed by a frame with an aperture at least for one cuvette of the matrix. The aperture includes a flexible clamping element that fastens the cuvette into the aperture with help of the friction, preferably by pressing its lower part from the sides. The clamping element, anyway, provides sufficient play so that the cuvette can be pushed into the aperture. The clamping element may press the cuvette from one side or several sides. According to one embodiment the clamping element presses the cuvette against a rigid frame. The clamping element may comprise one or more flexible fingers. The finger is preferably flexible horizontally.
FIGS. 1-4 illustrate one application of the invention adapted to a micro test plate comprising apertures 8×12.
FIGS. 1a and 1b illustrate a one-row cuvette matrix 1. The single cuvettes, i.e. wells 2 thereof are preferably connected to one another by narrow stems 3. The stems 3 are fixed near the top part of the wells. The stems 3 are placed at a distance from the centre line of the cuvette row at sides of the cuvette row, so that the stems 3 next to each other are each in opposite sides. The stems 3 provide sufficient play so that each distance between adjacent wells 2 can get smaller and wider for some hundredths or tenths of a millimeter.
The inside of the wells 2 is cylindrical. Their bottom forms a light transmission measuring window. The window is protected against scratching with a collar around the window.
On the outer surface of the wells 2, slightly below the middle there is a shoulder 5, broader than the lower part of the well, which determine how deep the well can be pushed into the tray 6. The outer surface of the lower part of the well 2 is cone-shaped, tapered slightly downwards.
The stems 3 can be broken by hand. This enables the required amount of wells 2 to be readily removed.
In the both ends of the cuvette matrix there are flanges 7 at the top part, that can also be broken off.
In the tray 6 in accordance with FIGS. 2a and 2b there are 8×12 apertures 8, in cross-sectional quadrangle shape. The side of the aperture 8 is slightly shorter than the biggest diameter of the lower part of the well 2. The apertures 8 form 8 rows, marked with letters (A-H) and 12 columns, marked with numbers (1-12) that is, 8×12. The apertures 8 are delimited by a rectangular frame with separation walls perpendicular to one another.
Separation walls parallel with the columns are integral and rigid. From the second separation wall on, from the side, every other wall parallel with the rows is also integral and rigid, the separation walls extending laterally and longitudinally across said frame.
From the first separation wall, from the side, each inward wall parallel with the rows is cut off or severed vertically at the centre line of the column, but diagonally against the separation wall and so that there is a small gap between the cut-off ends. The thus formed fingers 9, parallel with the separation walls of the rows, are slightly bent in horizontal direction. Thus, a well 2 can be pushed into each aperture 8, whereby the finger 9 bends away from the centre of the aperture. The finger 9 helps to keep the well 2 in the aperture with the aid of the friction.
The upper edge of the rigid separation walls 10 provides a stop for shoulder 5 on the outer surface of the well 2. Also on the sides of the tray the frame has ancons against the shoulders 5.
On the side of the tray there is a pin 11 at the lower end of each column. One head flange 7 of the cuvette matrix has a corresponding hole 12. Thus, the cuvette matrix is capable of being positioned the right way on the tray.
Lower edges of the tray extend lower than the bottoms of the wells 2 in the tray. Additionally, there are lips in the corners 13 of the tray to enable the trays to be readily piled.
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|U.S. Classification||422/552, 422/942, 220/23.4, 206/558, 220/23.86, 206/560, 220/23.83, 422/560|
|International Classification||B01L3/00, G01N35/02, G01N1/10, G01N33/48, B01L9/06, G01N33/53, G01N33/543|
|Cooperative Classification||B01L3/5085, B01L3/50855, B01L9/06|
|European Classification||B01L3/50855, B01L3/5085, B01L9/06|
|Aug 24, 1990||AS||Assignment|
Owner name: LABSYSTEMS OY, HELSINKI
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:TERVAMAKI, JUKKA;VAURAMO, KARI;REEL/FRAME:005434/0498
Effective date: 19900321
|Jun 1, 1993||CC||Certificate of correction|
|Aug 31, 1995||FPAY||Fee payment|
Year of fee payment: 4
|Aug 16, 1999||FPAY||Fee payment|
Year of fee payment: 8
|Sep 3, 2003||FPAY||Fee payment|
Year of fee payment: 12