|Publication number||US7339027 B2|
|Application number||US 10/449,609|
|Publication date||Mar 4, 2008|
|Filing date||Jun 2, 2003|
|Priority date||May 31, 2002|
|Also published as||CA2487291A1, EP1513549A2, EP1513549A4, US8304381, US20040127407, US20090088375, WO2003102148A2, WO2003102148A3|
|Publication number||10449609, 449609, US 7339027 B2, US 7339027B2, US-B2-7339027, US7339027 B2, US7339027B2|
|Inventors||Wen Y. Chen|
|Original Assignee||Ghc Research Development Corporation|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (8), Non-Patent Citations (36), Referenced by (2), Classifications (20), Legal Events (5)|
|External Links: USPTO, USPTO Assignment, Espacenet|
This application claims priority to U.S. Provisional Application No. 60/384,121, filed May 31, 2002 which is incorporated herein by reference in its entirety.
The present invention was supported in part by the Endowment Fund of the Greenville Hospital System and Grants (DAMD17-99-1-9129, DAMD17-01-1-0207, NIH/NCI 1R21CA87093).
The present invention relates generally to the methodology of preparing and using fusion proteins comprising a human prolactin antagonist and an angiogenesis inhibitor for use in treatment of cancers.
Human breast cancer is the predominant malignancy and the leading cause of cancer death in women from Western society, as reported by Miller et al., (eds) BIOLOGY OF FEMALE CANCERS, 31-42 (CRC Press, 1997). According to recent estimates from the American Cancer Society, one in every eight U.S. women will have breast cancer and the disease will kill 43,500 women in 1998.
Several lines of evidence have strongly linked prolactin (PRL) to breast cancer development. Expression levels of prolactin receptors (PRLR) reportedly are higher in human breast cancer cells than in normal breasts epithelial cells (Reynolds et al., 1997), or in surgically removed breast cancer tissues (Touraine, Martini P. et al., Increased Expression Of Prolactin Receptor Gene In Human Breast Tumors Versus Continguous Normal Breast Tissues, (Abstract) 79th Annual Meeting of Endocrine Society, p. 113, (1997)). PRLR levels in malignant breast tissue can be five-fold higher than in the surrounding normal tissue (see Touraine et al. (1997), supra, making the malignant cells highly sensitive to the stimulation by hPRL. Additionally, it has been suggested that one mechanism of the mitogenic action of estrogen in breast may influence the production and secretion of human prolactin (hPRL), since there is a positive correlation between PRLR, estrogen receptors or progesterone receptor levels (Sirbasku, 1978; Dixon and Lippman 1986; Lippman and Dickson, 1989). Taken together, these findings lead to a hypothesis that hPRL serves as an autocrine/paracrine growth factor that plays an important role in mammary carcinogenesis (Clevenger, et al., Am. J. Pathology, 146: 695-705 (1995); Ginsburg, E. et al., Cancer Res., 55: 2591-2595 (1995)).
An association between PRL expression and prostate disease has also been proposed in Wennbo et al., Endocrinol. 138: 4410-4415 (1997). PRL receptors are found in prostate tissue as reported Aragona et al., Endocrinol. 97: 677-684 (1975), and Leake et al., J. Endocrinol., 99: 321-328 (1983). In addition, PRL levels has observed that can increase with age (Hammond et al., Clin. Endocrinol., 7: 129-135 (1977), Vekemans et al., Br. Med. J. 4: 738-739 (1975)) coincident with the development of prostate hyperplasia. Transgenic mice overexpressing the PRL gene developed dramatic enlargement of the prostate gland. (see Wennbo et al. (1977), supra).
In view of its link to both breast and prostate cancer, PRL signaling represents an attractive target for therapeutic intervention. Heretofore, however, no suitable medicaments have been available for this purpose.
Inhibition of tumor angiogenesis has also been shown to hold great promise in treating cancer. Angiogenesis is a complex multi-step process that includes endothelial cell proliferation, migration, and differentiation, degradation of extracellular matrices, tube formation, and sprouting of new capillary branches (Tarui et al., 2001). Tumors often over-express several pro-angiogenic molecules, including members of fibroblast growth factor (FGF) and vascular endothelial growth factor families (VEGF, Kim et al., 1993; Cheng et al., 1996; Benjamin and Keshet 1997). Both vessel density and angiogenesis directly correlate with metastasis formation and prognosis (Vijayagopal et al., 1998; Guidi et al., 2000). Excessive angiogenesis is part of the pathology of cancer, and preventing angiogenesis in a tumor could effectively induce a dormant state in the tumor cells (Folkman, 1995; Hanahan and Folkman 1996). Blocking angiogenesis has demonstrated great promise as a therapeutic approach to treat or even eradicate cancer by cutting off its blood supply. Anti-angiogenesis therapy for cancer is effective because: (1) tumor growth is dependent on angiogenesis; (2) degree of angiogenesis is proportional to invasiveness of tumor; (3) tumor endothelial cells are qualitatively different from endothelial cells in adult non-neoplastics tissue; (4) endogenous inhibitors and stimulators of angiogenesis exist and have been isolated. Ryan and Wilding 2000. A number of unique biological effects make the angiogenesis inhibitors intriguing anticancer agents such as (1) acquired drug resistance may be less likely than with cytotoxic agents; tumor dormancy may be achieved through prolonged drug administration; (2) haematological toxicity is unlikely as often seen in chemotherapeutics; and (3) potential for synergy with cytotoxic agents.
Two important molecules that have the most promising affect on inhibiting angiogenesis are the soluble endogenous factors angiostatin and endostatin. Endostatin, a 20 kDa C-terminal fragment of collagen XVIII, was first characterized by O'Reilly et al. (1997) and has been reported to exhibit antiangiogenic and tumor-regressing activities (O'Reilly et al., 1997; Boehm et al., 1997). Angiostatin, a proteolytic fragment of plasminogen, has also been described to exert potent antiangiogenic and anti-tumor activities in a variety of tumor models (O'Reilly et al., 1994, 1996). The mechanisms by which endostatin and angiostatin inhibit angiogenesis are not known. Both endostatin and angiostatin are currently in early phase of clinical trials (see review by Herbst et al., 2001).
One of the most potent and specific angiogenic factors is VEGF (reviewed by Ferrara, 2001). VEGF and its high-affinity tyrosine kinase receptor Flk-1/KDR are central regulators of both physiological and pathological angiogenesis. The high expression level of VEGF and Flk-1 in the tumor endothelium indicates that this signal transduction system stimulates the proliferation and the survival of tumor vessels by a paracrine mechanism (Kim et al., 1993; Cheng 1996; Ferrara, 2001). Direct evidence for this hypothesis was provided by the inhibition of tumor growth in animal models by the application of VEGF neutralizing antibodies (Kim et al., 1993; Cheng 1996) or by the gene transfer of dominant negative Flk-1 receptor mutants (Millauer et al., 1994; 1996). Flk-1 expression is suppressed in adult endothelium, but is highly induced in the newly formed blood vessels in a variety of human tumors. Most recent studies using adenovirus as a delivery system to directly compare the efficacy of endostatin, angiostatin as well ligand binding ectodomains of VEGF receptors Flk-1 (Flk-1-BP) show that Flk-1-BP is a better angiogenesis inhibitor than either endostatin or angiostatin (Kuo et al., 2001).
Accordingly, it is an object of the invention to provide a medicament that is capable of interfering with the prolactin signaling mechanism in a cancer cell.
It is yet another object of the invention to provide a medicament that inhibits angiogenesis in the tumor cell lines.
It is still another object of the invention to provide a method for treating a patient suffering from cancer by simultaneously antagonizing a receptor present in a targeted cancer cell and inhbiting tumor angiogenesis.
It is another object of the invention to provide a method of treating cancer by employing the medicaments described herein.
These and other objects which will be more readily apparent upon reading the following disclosure may be achieved by the present invention.
In a composition of matter aspect, the present invention relates substantially to a protein comprising a receptor antagonizing domain and an angiogensis inhibiting domain. The invention further provides that the receptor antagonizing domain can be an apoptosis-promoting domain, while the angiogensis inhibiting domain can be endostatin. The receptor antagonizing domain also can be the amino acid sequence SEQ ID NO: 1 or conservative variants thereof.
In a methodological aspect, the present invention relates to a method for treating cancer, comprising administering to a patient an effective amount of a protein having a receptor-antagonizing domain and angiogensis inhibiting domain. The invention further provides a methodology for administering to a patient any of the proteins described herein.
It has been discovered by the present inventors that the combined effects of endocrine-based and targeted anti-angiogenesis therapies greatly enhance the treatment of cancer. For instance, compositions and methods of treatment disclosed herein act to inhibit the autocrine/paracrine effects of endogenous PRL by blocking the PRLR, typically resulting in apoptosis. In addition, this approach inhibits tumor angiogenesis, further inhibiting tumor growth.
As used herein, “apoptosis” refers to a process whereby developmental or environmental stimuli activate a genetic program to implement a specific series of events that culminate in the death and efficient disposal of a cell. The morphological changes in the cell include dramatic shrinkage of cell volume, accompanied by dilation of endoplasmic reticulum and convolution of the plasma membrane. In turn, this causes the cell to break up into a series of membrane-bounded bodies, containing structurally normal, yet compacted, organelles. The nucleus undergoes discontinuous chromatin condensation and nuclease-mediated DNA fragmentation occurs, degrading chromosomal DNA into small oligonucleosomal fragments. The nucleus and cytoplasm condense and the dying cell ultimately fragments into membrane-bound apoptotic bodies that are rapidly phagocytosed and digested by macrophages or by neighboring cells.
The present invention combines the benefits associated with blocking the PRLR and inhibiting angiogenesis by utilizing a multi-domain molecule, each domain having the ability to carry out one of these functions. Typical molecules have a “receptor-antagonizing domain” or an “apoptosis-promoting domain,” combined with a “angiogensis inhibiting domain.”
Since drug efficacy is affected by its serum half-life, one potential limitation of G129R alone, as well as endostatin alone in cancer treatment is their relative short serum half-life (Zhang et al., Clin. Cancer Res., 8:1196-1205 (2002)). The present in this study demonstrates that G129R-Endostatin has a three-fold longer serum half-life when compared to G129R alone While not wishing to be bound to a particular theory, the inventors believe that the enhanced in vivo anti-tumor effects of G129R-Endostatin, as compared to G129R and endostatin may also be attributed to the localization of endostatin portion of the fusion protein to the tumor site. The localized endostatin, in turn, results in inhibition of the surrounding endothelial cells that will form the tumor associated vascular network, and inhibition of breast cancer cell proliferation within the tumor mass.
As used herein, a “receptor-antagonizing domain” is a ligand that specifically binds to a receptor that is associated with a disorder like cancer, whereupon binding to the receptor, the receptor-antagonizing domain acts to inhibit one or more cellular processes, thereby interrupting the etiology or maintenance of the disease. Such a domain that induces apoptosis is herein referred to as the “apoptosis-promoting domain,” while a “angiogenesis inhibiting domain” is one that inhibits formation of the tumor neovasculature.
The benefits of a fusion protein having these characteristics are immense. For example, carcinogenic tissues are often characterized by increased levels of one or more protein receptors. A fusion protein containing a domain that is specific to one of these receptors will be able to specifically target the cancer tissue. Where the receptor antagonizing domain disrupts the etiology of the cancer, or disrupts cancer maintenance, as is the case of an apoptosis-promoting domain, the receptor antagonizing portion of the molecule has a direct therapeutic effect. In addition, due to the presence of the angiogenesis inhibiting domain, the molecule has a secondary therapeutic effect by inhibiting formation of the tumor neovasculature, thereby depriving the tumor of blood supply and the associated nutrients needed for tumor growth.
Accordingly, candidates to receive the therapy according to this invention include individuals who suffer from malignant tumors those of which are characterized by the presence of at least one receptor related to tumor maintenance or proliferation. In a preferred embodiment, the receptor-antagonizing domain of the fusion protein is an apoptosis-promoting domain, which binds to a targeted membrane-bound receptor. Such binding induces apoptosis; simultaneously, the angiogenesis inhibiting domain inhibits formation of the tumor neovasculature.
The Inventive Bi-Functional Protein
In accordance with the invention, bi-functional proteins are contemplated that have unique dual therapeutic effects on malignant tissue, namely (a) receptor-antagonizing and/or apoptosis-promoting (which may be one and the same) and (b) angiogenesis inhibition. The invention also contemplates nucleic acids (e.g. DNA or RNA) encoding the inventive bi-functional proteins.
The invention contemplates a first domain that, in one aspect, will localize the effects of the receptor antagonizing domain to the diseased tissue. For example, carcinogenic tissues are often characterized by increased levels of one or more protein receptors. A fusion protein containing a domain that is specific to one of these receptors will be able to specifically target the cancer tissue, resulting in a localized tumor cytotoxicity reaction directed to the targeted tissue.
In one embodiment, the domain that targets a particular receptor site is a receptor-antagonizing domain, which, as its name suggests, binds to and antagonizes its cognate receptor. As long as the receptor antagonizing domain recognizes a receptor that is highly expressed in cancer cells, it is suitable for use in the present invention. In a preferred embodiment, the receptor-antagonizing domain is an apoptosis-promoting domain.
An additional therapeutic benefit of this dual-function molecule is that the receptor-antagonizing domain typically has endocrine-blocking ability. Thus, where the receptor-antagonizing domain, for example, is a prolactin antagonist, the normal endocrine function of prolactin will be disrupted. As a consequence of this endocrine-blocking, in the case of prolactin and similar molecules, for instance, apoptosis of the targeted cells can result. In that case, the receptor-antagonizing domain is also an apoptosis-promoting domain.
In the case of an apoptosis-promoting domain, such a domain generally is designed by creating antagonists of the normal function of a cellular component that is involved in preventing apoptosis. In both breast and prostate cancer tissue, for example, carcinogenesis and malignant cell proliferation is stimulated, at least in part, by increased levels of PRLR. Signaling via the PRLR is known to be mediated by dimerization of the prolactin receptor, which is itself mediated by dimerization of receptor-bound prolactin molecules. The binding of endogenous PRL to two PRLRs induces PRLR dimerization, thereby triggering signal transduction into the cancer cells. Accordingly, one embodiment of the invention entails antagonizing the normal apoptosis-inhibiting function of prolactin using a prolactin antagonist (PRLA) (i.e., a prolactin antagonist domain).
Signal transduction in the PRLR signaling pathway involves signal transducers and activators of transcription (STAT) phosphorylation, which is involved in preventing or blocking apoptosis, the normal result of PRLR agonism. Thus, G129R antagonist promotes apoptosis by inhibiting STAT 5 phosphorylation in human breast cancer cells. Accordingly, blocking the PRLR inhibits the autocrine/paracrine effects of endogenous PRL, which involves STAT 5, and results in apoptosis. Thus, one class of apoptosis-promoting compounds contemplated by the invention is one that can inhibit STAT 5 phosphorylation.
A suitable PRLA contemplated by the invention generally will retain the characteristic of specific binding to the PRLR, yet will have some structural deficiency that disrupts the normal PRL apoptosis-blocking mechanism. Such a structural deficiency includes those that disrupt PRL (and thus PRLR) dimerization.
In one preferred embodiment, shown in SEQ ID NO: 1, this structural deficiency is a substitution of Gly to Arg at a position corresponding to 129 in hPRL (denoted as hPRL-G129R). As shown in
This embodiment is supported by Chen et al., Clin. Can. Res. 5: 3583-93 (1999), who disclose a species comparison of amino acid sequences within the third α-helical region of PRLs, shown in Table 1. (SEQ ID NOS 9-40, respectively, in order of appearance)
*Table 1 This table has been reproduced from Cooke et al. Journal of Biological Chemistry, 256:4007-4016 (1981). We note that this table provides that the amino acid in position number 128 of the human prolactin sequence is an arginine. However, our sequence data (see FIG. 1) and Genebank accession numbers BC015850, NM_00948, X54393, V00566, M29386, D00411 and U75583 indicate that amino acid number 128 in hPRL is a glutamic acid.
According to Table 1, it is clear that Gly 129 of hPRL is invariable among PRLs, suggesting an important role in its function. Thus, substituting any amino acid for Gly 129 should produce PRLA in each of these species (Chen et al., Molec. Endocrinol. (1995)). In one embodiment, an antagonist is created by substituting a relatively bulky side chain amino acid, such as Arg for Gly 129. Accordingly, one aspect of the invention contemplates conservative variants of PRL that are characterized by the presence of a relatively small side-chain amino acid (i.e. Gly) at a specific position, such that substituting the small side-chain amino acid for a bulky side-chain amino acid will result in an antagonistic form of the protein. In a preferred embodiment, the receptor antagonizing domain is a hPRL-G129R antagonist.
The receptor-antagonizing domain of present invention also includes conservative variants of receptor antagonizing domains discussed herein. The overall structure and composition of the inventive domains, in that respect, are important only insofar as they confer the appropriate functional characteristics, i.e., receptor antagonism, apoptosis induction, positive immunomodulation.
Conservative variants according to the invention generally conserve the overall molecular structure of the protein domains. Given the properties of the individual amino acids comprising the disclosed protein products, some rational substitutions will be apparent. Amino acid substitutions, i.e. “conservative substitutions,” may be made, for instance, on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues involved.
For example: (a) nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; (b) polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; (c) positively charged (basic) amino acids include arginine, lysine, and histidine; and (d) negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Substitutions typically may be made within groups (a)-(d). In addition, glycine and proline may be substituted for one another based on their ability to disrupt α-helices. Similarly, certain amino acids, such as alanine, cysteine, leucine, methionine, glutamic acid, glutamine, histidine and lysine are more commonly found in α-helices, while valine, isoleucine, phenylalanine, tyrosine, tryptophan and threonine are more commonly found in β-pleated sheets. Glycine, serine, aspartic acid, asparagine, and proline are commonly found in turns. Some preferred substitutions may be made among the following groups: (i) S and T; (ii) P and G; and (iii) A, V, L and I. Given the known genetic code, and recombinant and synthetic DNA techniques, the skilled scientist readily can construct DNAs encoding the conservative amino acid variants.
Conservative variants specifically contemplate truncations of the presently described receptor antagonizing domains. Truncations may be made from the N- or C-terminus, but generally do not entail deleting more than about 30% of the native molecule. More preferably, less than about 20%, and most preferably, less than about 10%, of the native molecule is deleted.
In general, both the DNA and protein molecules of the invention can be defined with reference to “sequence identity.” Some molecules have at least about 50%, 55% or 60% identity. Preferred molecules are those having at least about 65% sequence identity, more preferably at least 70% sequence identity. Other preferred molecules have at least about 80%, more preferably at least 85%, sequence identity. Most preferred molecules have at least about 90%, more preferably at least 95%, sequence identity. As used herein, two nucleic acid molecules or proteins are said to “share significant sequence identity” if the two contain regions which possess greater than 85% sequence (amino acid or nucleic acid) identity.
“Sequence identity” is defined herein with reference the Blast 2 algorithm, which is available at the NCBI website, using default parameters. References pertaining to this algorithm include: Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990) “Basic local alignment search tool.” J. Mol. Biol. 215: 403-410; Gish, W. & States, D. J. (1993) “Identification of protein coding regions by database similarity search.” Nature Genet. 3: 266-272; Madden, T. L., Tatusov, R. L. & Zhang, J. (1996) “Applications of network BLAST server” Meth. Enzymol. 266: 131-141; Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. (1997) “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.” Nucleic Acids Res. 25: 3389-3402; and Zhang, J. & Madden, T. L. (1997) “PowerBLAST: A new network BLAST application for interactive or automated sequence analysis and annotation.” Genome Res. 7: 649-656. Accordingly, the prolactin peptide sequences from different species, which include those listed in Table 1, can be aligned, using standard computer programs like BLAST, to inform further variation in prolactin-derived receptor-antagonizing domains that preserve their essential function.
In addition to proteins that are conservative variants of those disclosed herein, the invention also contemplates the use of proteins that play a role in inducing tumor proliferation, wherein an amino acid substitution will inhibit the protein's ability to induce this proliferation. For example, Gly 119 and Gly 120 of bovine growth hormone (bGH) and hGH, respectively, play critical roles in the action of GH in stimulating growth enhancement. Growth hormone receptor (GHR) dimerization is thought to be a key step for HG signal transduction. Accordingly, any amino acid substitution (other than Ala), especially one with a bulky side chain such as Arg at these respective positions will prevent receptor dimerization, resulting in a growth hormone antagonist (GHA). Thus, antagonists such as GHA are contemplated by the invention. In a preferred embodiment, the receptor antagonizing domain is a hGH-G120R antagonist.
Angiogenesis Inhibiting Domain
The invention also contemplates an additional, yet separate, domain that acts as an angiogenesis inhibitor. Preferred angiogenesis inhibiting domains include endostatin. In addition to endostatin, the invention contemplates other molecules, including proteins, that are capable of inhibiting the formation of the tumor neovasculature. For example, endostatin, angiostatin, and Flk-1 bp are contemplated in the present invention.
Flk-1 is a full length receptor that binds VEGF. Flk-1 bp is the extracellular portion of the Flk-1 receptor that is responsible for binding to VEGF. Flk-1 bp instead of Flk-1 is used in the present invention in order to avoid insertion into the cell membrane.
The invention also includes conservative variants (as detailed above) of the aforementioned angiogenesis inhibiting domains.
Preparing Exemplary Bi-Functional Molecules
A bi-functional protein contemplated by this invention is one that contains each of the previously mentioned domains, namely receptor-antagonizing (which also may be apoptosis-promoting) and angiogenesis inhibiting, wherein upon such fusing, both domains substantially retain their associated characteristics, independent of the other.
In one embodiment, the fusion protein is a hPRLA-endostatin protein, as shown in
Suitable methods for creating the fusion protein should be ones that do not substantially change the biological activity of either of these domains. For example, it has been demonstrated that fusion of the N-terminal of IL-2 to the C-terminal end of an antibody does not change the biological activity of IL-2 Reisfeld et al. (1996), supra. Therefore, a similar strategy can be adopted to produce a fusion protein according to the invention. This process includes designing a cDNA encoding a fusion protein which links the N-terminus of the positive immunomodulator domain to the C-terminus of receptor-antagonizing domain.
Moreover, there is evidence that the C-terminal ends of hGH (we deleted up to 10 amino acids) are not important for growth promoting activities in transgenic mice (Chen et al., 1993) and, based on structural similarity, fusion of a positive modulator to the C-terminal end of other receptor-antagonizing domains, such as hPRLA, should not alter the binding affinity of these domains.
The present invention is not limited to any particular method of producing the desired fusion protein contemplated herein. According to the contemplated recombinant methods of production, however, the invention provides recombinant DNA constructs comprising one or more of the nucleotide sequences of the domains described in the present invention. The recombinant constructs of the present invention comprise a vector, such as a plasmid or viral vector, into which a DNA or DNA fragment, typically bearing an open reading frame, is inserted, in either orientation. The invention further contemplates cells containing these vectors.
Recombinant protein production is well known in the art and is outlined briefly below.
Useful expression vectors for bacterial use are constructed by inserting a structural DNA sequence encoding a desired protein together with suitable translation initiation and termination signals in operable reading phase with a functional promoter. The vector will comprise one or more phenotypic selectable markers and an origin of replication to ensure maintenance of the vector and, if desirable, to provide amplification within the host. Suitable prokaryotic hosts for transformation include E. coli, Bacillus subtilis, Salmonella typhimurium and various species within the genera Pseudomonas, Streptomyces, and Staphylococcus, although others may, also be employed as a matter of choice. In a preferred embodiment, the prokaryotic host is E. coli.
Bacterial vectors may be, for example, bacteriophage-, plasmid- or cosmid-based. These vectors can comprise a selectable marker and bacterial origin of replication derived from commercially available plasmids typically containing elements of the well known cloning vector pBR322 (ATCC 37017). Such commercial vectors include, for example, GEM 1 (Promega Biotec, Madison, Wis., USA), pBs, phagescript, PsiX174, pBluescript SK, pBs KS, pNH8a, pNH16a, pNH18a, pNH46a (Stratagene); pTrc99A, pKK223-3, pKK233-3, pKK232-8, pDR540, and pRIT5 (Pharmacia). A preferred vector according to the invention is the Pt71 expression vector (Paris et al., Biotechnol. Appl. Biochem. 12: 436-449 (1990)).
These “backbone” sections are combined with an appropriate promoter and the structural sequence to be expressed. Bacterial promoters include lac, T3, T7, lambda P
Following transformation of a suitable host strain and growth of the host strain to an appropriate cell density, the selected promoter is derepressed/induced by appropriate means (e.g., temperature shift or chemical induction) and cells are cultured for an additional period. Cells are typically harvested by centrifugation, disrupted by physical or chemical means, and the resulting crude extract retained for further purification.
Various mammalian cell culture systems can also be employed to express recombinant protein. Examples of mammalian expression systems include selected mouse L cells, such as thymidine kinase-negative (TK) and adenine phosphoribosul transferase-negative (APRT) cells. Other examples include the COS-7 lines of monkey kidney fibroblasts, described by Gluzman, Cell 23: 175 (1981), and other cell lines capable of expressing a compatible vector, for example, the C127, 3T3, CHO, HeLa and BHK cell lines. Mammalian expression vectors will comprise an origin of replication, a suitable promoter and enhancer, and also any necessary ribosome binding sites, polyadenylation site, splice donor and acceptor sites, transcriptional termination sequences, and 5′ flanking non-transcribed sequences. DNA sequences derived from the SV40 viral genome, for example, SV40 origin, early promoter, enhancer, splice, and polyadenylation sites may be used to provide the required non-transcribed genetic elements.
Mammalian promoters include CMV immediate early, HSV thymidine kinase, early and late SV40, LTRs from retrovirus, and mouse metallothionein-I. Exemplary mammalian vectors include pWLneo, pSV2cat, pOG44, pXT1, pSG (Stratagene) pSVK3, pBPV, pMSG, and pSVL (Pharmacia). In a preferred embodiment, the mammalian expression vector is pUCIG-MET. Selectable markers include CAT (chloramphenicol transferase).
In mammalian host cells, a number of viral-based expression systems may be utilized. In cases where an adenovirus is used as an expression vector, the coding sequence of interest may be ligated to an adenovirus transcription/translation control complex, e.g., the late promoter and tripartite leader sequence. This chimeric gene may then be inserted in the adenovirus genome by in vitro or in vivo recombination. Insertion in a non-essential region of the viral genome (e.g., region E1 or E3) will result in a recombinant virus that is viable and capable of expressing a target protein in infected hosts. (E.g., See Logan et al., 1984, Proc. Natl. Acad. Sci. USA 81: 3655-3659).
The proteins of the present invention can be formulated according to known methods to prepare pharmaceutically useful compositions, whereby the inventive molecules, or their functional derivatives, are combined in admixture with a pharmaceutically acceptable carrier vehicle. Suitable vehicles and their formulation, inclusive of other human proteins, e.g., human serum albumin, are described, for example, in Remington's Pharmaceultical Sciences (16th ed., Osol, A., Ed., Mack, Easton Pa. (1980)). To form a pharmaceutically acceptable composition suitable for effective administration, such compositions will contain an effective amount of one or more of the proteins of the present invention, together with a suitable amount of carrier vehicle.
Pharmaceutical compositions for use in accordance with the present invention may be formulated in conventional manner using one or more physiologically acceptable carriers or excipients. Thus, the bi-functional molecules and their physiologically acceptable salts and solvate may be formulated for administration by inhalation or insufflation (either through the mouth or the nose) or oral, buccal, parenteral or rectal administration.
For oral administration, the pharmaceutical compositions may take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g., pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g., lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g., magnesium stearate, talc or silica); disintegrants (e.g., potato starch or sodium starch glycolate); or wetting agents (e.g., sodium lauryl sulphate). The tablets may be coated by methods well known in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they maybe presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g., methyl or propyl-p-hydroxybenzoates or sorbic acid). The preparations may also contain buffer salts, flavoring, coloring and sweetening agents as appropriate.
Preparations for oral administration may be suitably formulated to give controlled release of the active compound. For buccal administration the composition may take the form of tablets or lozenges formulated in conventional manner.
For administration by inhalation, the bi-functional molecules for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of, e.g. gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.
The bi-functional proteins may be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g., in ampules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.
The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter or other glycerides.
In addition to the formulations described previously, the bi-functional molecules may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack may for example comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration.
The compositions, since they are useful in cancer treatment, may be formulated in conjunction with conventional chemotherapeutic agents. Conventional chemotherapeutic agents include alkylating agents, antimetabolites, various natural products (e.g., vinca alkaloids, epipodophyllotoxins, antibiotics, and amino acid-depleting enzymes), hormones and hormone antagonists. Specific classes of agents include nitrogen mustards, alkyl sulfonates, nitrosoureas, triazenes, folic acid analogues, pyrimidine analogues, purine analogs, platinum complexes, adrenocortical suppressants, adrenocorticosteroids, progestins, estrogens, antiestrogens and androgens. Some exemplary compounds include cyclophosphamide, chlorambucil, methotrexate, fluorouracil, cytarabine, thioguanine, vinblastine, vincristine, doxorubincin, daunorubicin, mitomycin, cisplatin, hydroxyurea, prednisone, hydroxyprogesterone caproate, medroxyprogesterone, megestrol acetate, diethyl stilbestrol, ethinyl estradiol, tomoxifen, testosterone propionate and fluoxymesterone. In treating breast cancer, for example, tamoxifen is particularly preferred.
The inventive therapeutic methods according to the invention generally utilize the bi-functional proteins identified above. The domains of the fusion proteins share the ability to specifically target a specific tissue and/or augment an immune response to targeted tissue. Accordingly, a typical method, involves binding a receptor of a target cell by the receptor-antagonizing domain of the fusion protein and/or inhibiting formation of tumor neovasculature via the angiogenesis inhibiting domain.
For example, the instant invention describes that a novel fusion protein, such as GEFP, is able to bind to the PRL receptor (PRLR) on T-47D human breast cancer cells, for example, and inhibit the signal transduction induced by PRL. At the same time, GEFP is able to inhibit human umbilical vein endothelial cell (HUVEC) proliferation and disrupt the formation of endothelial tube structures with potency similar to that of endostatin. Therapeutic methods involve administering to a subject in need of treatment a therapeutically effective amount of a fusion protein. “Therapeutically effective” is employed here to denote the amount of fusion proteins that are of sufficient quantity to inhibit or reverse cancer growth (e.g., induce apoptosis). Some methods contemplate combination therapy with known cancer medicaments or therapies, for example, chemotherapy (preferably using compounds of the sort listed above) or radiation. The patient may be a human or non-human animal. A patient typically will be in need of treatment when suffering from a cancer characterized by increased levels of receptors that promote cancer maintenance or proliferation.
Administration during in vivo treatment may be by any number of routes, including parenteral and oral, but preferably parenteral. Intracapsular, intravenous, intrathecal, and intraperitoneal routes of administration may be employed, generally intravenous is preferred. The skilled artisan will recognize that the route of administration will vary depending on the disorder to be treated.
Determining a therapeutically effective amount of the bi-functional protein, according to this invention, largely will depend on particular patient characteristics, route of administration, and the nature of the disorder being treated. General guidance can be found, for example, in the publications of the International Conference on Harmonisation and in REMINGTON'S PHARMACEUTICAL SCIENCES, chapters 27 and 28, pp. 484-528 (Mack Publishing Company 1990).
Determining a therapeutically effective amount specifically will depend on such factors as toxicity and efficacy of the medicament. Toxicity may be determined using methods well known in the art and found in the foregoing references. Efficacy may be determined utilizing the same guidance in conjunction with the methods described below in the Examples. A pharmaceutically effective amount, therefore, is an amount that is deemed by the clinician to be toxicologically tolerable, yet efficacious. Efficacy, for example, can be measured by the decrease in mass of the targeted tissue. Suitable dosages can be from about 1 mg/kg to 10 mg/kg.
Screening Assays to determine the biological activities of the fusion protein
The present invention also provides cell-based assay systems that can be used to compare the biological activities of the receptor antagonizing domain and angiogenesis inhibiting domain, respectively, and/or a fusion protein comprising each of these domains. To this end, antibody binding assays are used to ensure that the fused domains of the fusion protein are being expressed. Competitive binding assays may be used to ensure that each domain of the fusion protein binds to its normal receptor.
By introducing to a cell line various concentrations of a particular domain in its antagonized, non-antagonized, and fused forms, one of skill in the art could determine the biological activity of the apoptosis-promoting domain of the fused protein vis-à-vis the same domain in its non-fused state. There are numerous ways to measure apoptosis. These methods include, but are not limited to the following techniques: (1) Loss of cell viability—measured by a failure to either exclude vital dye or uptake MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), or MTS-PMS; (2) DNA fragmentation—assayed by agarose gel electrophoresis, PFG electrophoresis, in situ terminal transferase labeling (TUNEL); Cell and nuclear morphology—employing microscopy to visualize chromatin condensation, DNA organization, and cytoplasmic integrity; and Cysteine protease activation assays—utilizing caspase activation assays combined with colorimetric or fluorescent readouts, poly (ADP-ribose) polymerase (PARP) or laminin cleavage by western blot or immunohistochemisrty.
Likewise, a cell line that can measure activity of the angiogenesis inhibiting domain should be similarly used to monitor the activity of this domain of the fusion protein. Examples 7 and 8, which use human umbilical vein endothelial cell proliferation and organization assays, are possible, yet non-limiting methods to determine biological activity of the angiogenesis inhibiting domain in the fusion protein.
The following examples are intended to be illustrative and not limiting.
A two-step cloning procedure based upon published protocols (Catado et al., 2000) was used to generate recombinant DNA encoding hPRL-G129R fused to endostatin, angiostatin or Flk-1-BP, making a G129R-endostatin (G129R-endo), G129R-angiostatin, and G129R-Flk-1-bp fusion protein, respectively. Human endostatin, angiostatin, or Flk-1-bp were PCR amplified from Universal Quick-Clone cDNA (Clontech, CA). Each of the amplified cDNA fragments were first be cloned independently into a pCR2.1 TA cloning vector (Invitrogen, Inc.; Carlsbad, Calif.) and their sequences were confirmed.
For instance, Primers corresponding to G129R (5′ primer; restriction site for NdeI: 5′-CAT ATG TTG CCC ATC TGT CCC GGC-3′ (SEQ ID NO: 41) and 3′ primer; restriction site for BamHI: 5′-GGA TCC GCA GTT GTT GTT GTG GAT-3′ (SEQ ID NO: 42)) were used to amplify the G129R fragment from a previous clone (Chen et al., Clin. Cancer Res., 5:3583-3593 (1999)). Primers corresponding to human endostatin (5′ primer; restriction site for BamHI: 5′-GGA TCC CAC AGC CAC CGC GAC TTC CAG-3′ (SEQ ID NO: 43) and 3′ primer; restriction site XhoI with stop codon: 5′-CTC GAG CTA CTT GGA GGC AGT CAT GAA GC-3′ (SEQ ID NO: 44) were used to amplify the gene from a Human Universal QUICK-Clone cDNA library (Clontech, Palo Alto, Calif.). Another 5′ primer, NdeI: 5′-CAT ATG CAC AGC CAC CGC GAC TTC GAG, (SEQ ID NO: 45) was used with the XhoI 3′ primer for expression of human endostatin alone. All cDNA fragments were ligated separately into the TA cloning vector pCR2.1 (Invitrogen, Inc., Carlsbad, Calif.), restriction mapped and sequenced.
The cloned cDNA fragments were then re-isolated by restriction digestion, purified and ligated into the pET22b+ expression vector (Novagen, Madison, Wis.;
E. coli, such as BL21(DE3) cells (Novagen, Madison, Wis.), were transformed with plasmids encoding G129R fusion proteins using the calcium chloride method. Bacteria were allowed to grow overnight in Luria-Bertani (LB) broth (ampicillin 50 μg/ml) at 37 C. The following day a LB growth culture was generated by inoculation of 5% of the seed culture and grown for ≈2.5 hours at 37° C. with agitation.
Cells were transformed and grown as described in Example 2. IPTG (Fisher Scientific) was added to the culture (1 mM final concentration) to induce expression of G129R fusion proteins and incubated for an additional 4 hours. Bacteria were pelleted and resuspended in a solution containing 0.2M NaPO4 pH 8, 10 mM EDTA, and 0.5% Triton X-100. The resuspended bacteria were lysed with a 550 Sonic Dismembrator (Fisher Scientific). The products, which were present in the form of inclusion bodies, were pelleted at 12,000 g for 15 minutes and resuspended in 0.2M NaPO4 pH7, 1% v/v beta mercaptoethanol, and 8M Urea for refolding. The refolding process consists of dialyzing the protein against decreasing amounts of urea and beta-mercaptoethanol in the presence of 50 mM NH4HCO3 pH 8.0 for three consecutive days. The sample was first filtered through a 0.22 micron filter (VWR), degassed and then purified by Sepharose ionic exchange columns followed by Sephacryl gel filtration columns (Pharmacia, Piscataway, N.J.) using a FPLC system (Pharmacia, Piscataway, N.J.).
The samples of the purified proteins were run on a 12% SDS-PAGE gel and stained with Coumassie Blue. As shown in
All proteins used in this project were purified using two column procedures, i.e. ionic exchange followed by size exclusion columns using a FPLC system. The purified proteins were tested for endotoxin levels through Cape Cop Inc. The endotoxin levels were approximately 5EU/mg protein, which is considered acceptable for mouse studies.
Radioreceptor binding assays were performed as previously described (Chen et al., 1991; Appendix B). Briefly, T-47D cells will be grown in six-well tissue culture plates until 90% confluent (˜105 cells/well). Monolayers of cells were starved in serum-free RPMI-1640 medium for 2 h. The cells were then incubated at room temperature for two hours in serum-free RPMI-1640 containing 8×104 cpm 125I hPRL (Specific Activity=30 μCi/μg; NEN Dupont, Boston, Mass.) with or without hPRL, hPRL-G129R, endostatin, or GEFP at various concentrations. Cells were then washed three times in serum-free RPMI-1640 and solubilized in 0.5 ml of 0.1N NaOH/1% SDS, and the bound radioactivity was determined by a Gamma counter (ICN Biomedical, model 4/600plus; Costa Mesa, Calif.). The resulting data are shown in
These data show that PRL, G129R and G129R-Endostatin all competitively displaced the 125I-hPRL from the PRLR on T-47D cells with similar affinity, whereas endostatin did not, suggesting that G129R-Endostatin retained its PRLR recognition ability.
T-47D cells were grown in RPMI-1640 medium containing 10% Charcoal Stripped Fetal Bovine Serum (CSFBS; growth medium). For each experiment, cells were passed into 6 well culture plates in growth medium and cultured to 90% confluency. On the day of the experiment, cells were depleted in serum free media for one hour and incubated in hPRL, G129R or combination of two for 30 min. After treatment, T47-D cells were washed once with ice-cold PBS and collected by gentle scraping in 1 ml ice-cold lysis buffer [20 mM Tris-Cl (pH 7.4), 100 mM NaCl, 2 mM EDTA, 1% NP-40, 1 mM phenylmethylsulfonyl fluoride, 10 ug/ml aprotinin, 10 ug/ml leupeptin]. The lysis mixture was then passed through a 22 gauge needle several times avoiding air bubbles and centrifuged at 12,000×g for 20 minutes. The supernatant was then transferred to a new microcentrifuge tuber. Five μg of STAT5 monoclonal antibody will then be added to 100 microliters (200-500 micrograms total protein) of cell lysate along with 400 microliters of ddH2O and 500 microliters of 2×IP buffer [1% Triton X-100, 150 mM NaCl, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM EGTA, 0.2 mM sodium vanadate, 0.2 mM PMSF, 0.5% NP-40] to each reaction. After overnight incubation at 4° C. and gentle rotation, 50 microliters of prewashed (1×IP buffer) protein A agarose beads were added to each IP reaction and continue the Incubation for another 2 hours at 4° C. The agarose beads were washed 3× with 1×IP buffer and the protein will then be eluted by resuspending the protein A agarose beads in 50 microliters of 1×SDS PAGE loading buffer. Samples were then be subjected to 4-12.5% SDS-PAGE and immune blot analysis using horse radish peroxidase (HRP)-conjugated anti-phosphotyrosine antibody PY20 and ECL reagent kit (Amersham, IL). As shown in
STAT5 phosphorylation is one indicator of PRL-mediated signal transduction in mammary cells, and we have used STAT5 phosphorylation assay as a measure of the antagonistic effects of G129R and its variants (Cataldo et al., Int. J. Oncol., 17:1179-1185 (2000)). The status of STAT5 phosphorylation was examined by treating T-47D cells with PRL, G129R, endostatin and G129R-Endostatin. As shown in
T-47D and HUVEC cells were maintained as previously described. Cells were passed onto Lab-Tek Chamber Slide System (Fisher Scientific) and grown to approximately 70% confluency. HUVEC cells were cultured in low serum medium (2% FBS) and T-47D cells were serum depleted for 30 min. Cells were treated with either 10 g/ml of G129R, 10 g/ml of endostatin or 20 g/ml of G129R-Endostatin for 30 min at 37° C. Cells were treated in their respected serum-free media, and all staining was performed in triplicate and repeated at least twice. After treatment, cells were washed with phosphate buffered saline (PBS) [NaCl 120 mmol; KCl 2.7 mmol; phosphate buffer salts 10 mmol; pH=7.4], fixed with 4% Para-Formaldehyde (BD Biosciences, Bedford, Mass.) for 25 min at 4° C. and permeabilized with 0.2% Triton-X-100 in 1×PBS. Cells were incubated in blocking buffer for 30 min with 2% Bovine Serum Albumin (BSA; Fisher Scientific). Cells were incubated with the primary antibodies rabbit anti-human Endostatin (Ab-2), 1:200, and mouse anti-human PRL antiserum, 1:1000, at room temperature for 2 h. After incubation, cells were washed three times with 1% BSA/PBS and subjected to secondary antibody (1:500) incubation for 2 h at room temperature using Alexa Fluor 594 goat anti-mouse IgG (red fluorescence) and Alexa Fluor 488 goat anti-rabbit IgG (green fluorescence) (Molecular Probes, Inc., Eugene, Oreg.), respectively. Cells were rinsed twice with 1% BSA/PBS and incubated with Anti-Fade equilibrium buffer (10 l/well) (Molecular Probes) for 10 min at room temperature. The chambers were then removed and cover slides were mounted for observation. All wells were examined under an Olympus I×70 fluorescent microscope using 488 mm and 594 nm wavelengths. Digital photographs were taken at 450× magnification.
An immunofluorescence assay was used to determine if G129R-Endostatin has the ability to bind to both breast cancer and endothelial cells (
HUVEC's and T-47D cells were grown in their respective phenol-red free medium. Fully confluent HUVEC and T-47D cell cultures were trypsinized and cells were resuspended in medium containing 5% FBS. Cells were then seeded into 96-well culture plates at a density of 5,000 HUVEC's/well (in the presence of 2.5 ng/ml of basic fibroblast growth factor (bFGF) (Sigma) in the presence of 1 μg/ml of heparin (Sigma)) and 15,000 T-47D cells/well. After an incubation of 24 h, various concentrations of G129R, endostatin, or G129R-Endostatin were added to the appropriate well. Cells were further incubated for 72 h at 37° C. in a humidified 5% CO2 incubator. The viability of the cells was determined using the MTS-PMS (CellTiter 96 Aqueous Kit; Promega Corp., Madison, Wis.) calorimetric assay (following the manufacturer's instructions), and absorbance at 490 nm was determined using a microplate reader (Bio-Rad). Cell survival was calculated as a percentage of the control values. All experiments were carried out in triplicate.
Cell proliferation assays were carried out to examine the dual effects of G129R-Endostatin in inhibiting the proliferation of both HUVEC and T-47D cells. G129R-Endostatin revealed to be as effective as endostatin in inhibiting the proliferation of HUVEC cells in a dose dependent manner (
In another experiment, the cell proliferation assay for HUVEC cells followed the procedure described by Bae et al. Briefly, HUVEC cells were seeded in gelatin-coated 48-well plates at a density of 1×105 cells/well in 300 μL of growth media and were incubated for 2 days at 37° C. Plates were washed 3× with warm, serum-free growth medium before 200 μL of serum-free growth medium was added to the negative control and normal growth media was used for the positive control, as well as for the experimental samples. In the experimental samples, 500 ng/mL of protein was tested. These plates were allowed to incubate a day at 37° C. Next, 0.5 μCi of methyl-[3H]thymidine in 20 uL was added to each well and allowed to incubate a day at 37° C. The plates were then washed 4× with PBS containing 0.1% BSA, and the cells were solubilized with 150 μL 0.4N NaOH at room temperature for 20 min. To neutralize this reaction, 30 μL of 2N HCl was added to each well, and the contents were transferred to scintillation vials to determine the radioactivity of each sample in a scintillation counter. This experiment was carried out in triplicate and the resulting data is shown in
This assay generally followed a known procedure. (Morales et al. 1995) HUVECs were maintained in Leibovitz's medium containing 10% FBS. Prior to culture, 24-well culture plates were prepared by coating wells with Matrigel (polymerization for 30 min at 37° C.). HUVEC (1×105) suspended in 1 ml of Leibovitz medium, with or without the GEFP along with controls, was added into each well. Cells were incubated at 37° C. overnight. After removal of the culture medium, the culture was fixed with Diff Quick (Dade Behring Inc. Newark, Del.). The area of the endothelial tube network in each culture was examined microscopically by the Optomax-Olympus microscope (
Prominent tubal structures were demonstrated by the control cells (
Eight athymic nude mice were inoculated subcutaneously with 1×106 T-47D cells mixed with Matrigel. Tumors were allowed to grow for 21 days. Mice were randomized and divided into two equal groups. One group was injected with GEFP (10 mg/kg/mouse); the other group was injected with 100 μl of sterile saline (speckled) for 25 consecutive days. Tumor volume, calculated on day 12 and day 25 post treatment using the equation: [(short dimensions)×(long dimension)]/2, is shown in
Female Balb/c mice (Jackson Lab, Bar Harbor, Me.) were used to determine the serum half-life of G129R-Endostatin. Two hundred micrograms of G129R or 200 g of G129R-Endostatin was injected (i.p.) into Balb/c mice (n=4). Blood samples were obtained from each mouse at time intervals of 2, 4, 8, and 24 h by tail vein bleeding. Samples were placed on ice and immediately centrifuged for 5 min at 4° C. The serum was collected and frozen at −20° C. until further use. The serum concentration of both G129R and G129R-Endostatin was determined using the hPRL IRMA kit (DPC, Inc.). Area under the curve (AUC) was calculated by linear trapezoidal method from 0 to 24 h.
It has been demonstrated that increasing the size of a protein may increase its half-life (Zhang et al., Clin. Cancer Res., 8:1196-1205 (2002)). The relative short serum half-life of G129R and endostatin present a considerable challenge to the clinical use of these potential therapeutic agents. To examine whether the pharmacokinetics, namely half-life, of G129R-Endostatin are increased compared to G129R alone the relative serum half-life was assessed. As shown in
Two breast cancer cell lines, T-47D and 4T1, were used to investigate the anti-tumorigenic effects of a hPRL-G129R-Endo fusion protein. 8 control animals were treated with G129R-endostatin fusion protein after nude mice were innoculated with T47D tumor cells (n=4) or 4T1 tumor cells (n=4). The results are depicted in
The therapeutic efficacy of GEFP was confirmed using a mouse breast cancer cell line 4T1 in vivo.
In vivo studies were further considered and results are provided in
Among the four treatment groups, G129R-Endostatin (807±235 mm3) demonstrated the best inhibitory effects on 4T1 tumor growth compared to control group (2,851±305 mm3), G129R (1,897±194 mm3), endostatin (1,271±142 mm3), and the combination treatment (1,399±147 mm3) (FIG. 15A). The final tumor weights at the end of the experiment were: G129R-Endostatin (841±121 mg); control (1,970±410 mg), G129R (1,409±265 mg), endostatin (1,159±170 mg), and the combination of G129R and endostatin (1,149±195 mg) (
These examples are provided for illustrative purposes are not intended to limit the scope of the invention in any manner. It will be recognized by one of skill in the art that fusion proteins within the scope of the present invention may be created by a variety of means without departing from the spirit or scope of the present invention.
|Cited Patent||Filing date||Publication date||Applicant||Title|
|US5854205 *||Oct 22, 1996||Dec 29, 1998||The Children's Medical Center Corporation||Therapeutic antiangiogenic compositions and methods|
|US6114305 *||May 3, 1995||Sep 5, 2000||Institute National De La Recherche Agronomique||Utilization of prolactin for preventing and/or treating the expression of brooding behavior in birds|
|US6342221||Apr 28, 2000||Jan 29, 2002||Board Of Regents, The University Of Texas System||Antibody conjugate compositions for selectively inhibiting VEGF|
|US6803211 *||Aug 24, 2001||Oct 12, 2004||Pfizer Inc.||Methods and compositions for diagnosing and treating disorders involving angiogenesis|
|US20020068043 *||Mar 23, 2001||Jun 6, 2002||Chen Wen Y.||Bi-functional cancer treatment agents|
|WO1999016889A1 *||Sep 30, 1998||Apr 8, 1999||Searle & Co||Fusion proteins comprising an angiostatin moiety and their use in anti-tumor treatment|
|WO1999046371A2||Mar 11, 1999||Sep 16, 1999||Elizabeth M Bruckheimer||Induction of apoptotic or cytotoxic gene expression by adenoviral mediated gene codelivery|
|WO2001070985A2 *||Mar 23, 2001||Sep 27, 2001||Wen Y Chen||Bi-functional cancer treatment agents|
|1||"Influence of Age on Serum Prolactin Levels in Women and Men", British Medical Journal, vol. 4, No. 5999, pp. 738-739, Dec. 1975.|
|2||Altschul et al., "Basic Local Alignment Search Tool", Journal of Molecular Biology, vol. 215, No. 3, pp. 403-410, Oct. 1990.|
|3||Altschul et al., "Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Progams", Nucleic Acids Research, vol. 25, No. 17, pp. 3389-3402, Sep. 1997.|
|4||Aragona et al., "Specific Prolactin Binding Sites in the Prostate and Testis of Rats", Endocrinology, vol. 97, No. 3, pp. 677-684, Jan. 1975.|
|5||Beck et al., "Prolactin Antagonist-Endostatin Fusion Protein as a Targeted Dual-Functional Therapeutic Agent for Breast Cancer," vol. 63, No. 13, Jul. 2003, pp. 3598-3604.|
|6||Chen et al., "A Human Prolactin Antagonist, hPRL-G129R, Inhibits Breast Cancer Cell Proliferation through Induction of Apoptosis", Clinical Cancer Research, vol. 5, pp. 3583-3593, Nov. 1999.|
|7||Chen et al., "In vivo Studies of the Anti-Tumor Effects of a Human Prolactin Antagonist, HPRL-G129R," vol. 20, Apr. 2002, pp. 813-818.|
|8||Clevenger et al., "Expression of Prolactin and Prolactin Receptor in Human Breast Carcinoma", American Journal of Pathology, vol. 146 No. 3, pp. 695-705, Mar. 1995.|
|9||*||Curti, Crit. Rev. in Oncology/Hematology, 1993, 14:29-39.|
|10||Database DISSABS on STN, AN 2003:.7678, Beck et al., "Relationship of the prolactin antagonist, G129R, with Bc1-2 and its therapeutic effects as a fusion protein with endostatin for the treatment of breast cancer", Dissertation Abstracts International, 2002, vol. 63, No. 11B, p. 5056.|
|11||Eskes et al. Mol. Cell. Biol., 20(3):929-935 (2000).|
|12||Esposti, M.D., Apoptosis, 7(5):433-440 (2002).|
|13||Ginsburg et al., "Prolactin Synthesis and Secretion by Human Breast Cancer Cells", Cancer Research, vol. 55, No. 11; pp. 2591-2595, Jun. 1995.|
|14||Gish et al., "Identification of Protein Coding Regions by Database Similarity Search", Nature Genetics, vol. 3, No. 3, pp. 266-272, Mar. 1993.|
|15||*||Gura, Science, 1997, 278:1041-1042.|
|16||Hammond et al., "Serum FSH, LH and Prolactin and Nornal Males and Patients with Prostatic Diseases", Clinical Endocrinology, vol. 7, No. 2, pp. 129-135, Aug. 1977.|
|17||Hu et al. Apoptosis, 8(3):277-289 (2003).|
|18||*||Jain, Sci. Am., 1994, 271:58-65).|
|19||Leake et al., "Characterization of the Prolactin Receptor in Human Prostate", Journal of Endocrinology, vol. 99, pp. 321-328, Apr. 1983.|
|20||Letai et al. Cancer Cell, 2(3):183-192 (2002).|
|21||Liu et al. Biochem. Biophys. Res. Commun., 330(3):865-870 (2005).|
|22||Llovera et al., "Human Protein (HPRL) Antagonists Inhibit HPRL-Activated Signaling Pathways Involved in Breast cancer Cell Proliferation," vol. 19, No. 41, Sep. 2000, pp. 4695-4705.|
|23||Lutter et al. Nat. Cell Biol., 2(10):754-756 (2000).|
|24||Madden et al., "Applications of Network BLAST Server", Methods in Enzymology, vol. 266, pp. 131-141, 1996.|
|25||Malonne et al., "Mechanisms of Tumor Angiogenesis and Therapeutic Implications: Angiogenesis Inhibitors," vol. 17, No. 1, 1999, pp. 1-14.|
|26||Osol, Arthur, Remington's 16<SUP>th </SUP>Edition, Pharmaceutical Sciences, 1980. (Table of Contents).|
|27||Partial European Search Report for EP 02 79 9347, mailed Jul. 26, 2006.|
|28||*||Pidgeon et al, Br. J Cancer 85:273-278, Jul. 20, 2001.|
|29||*||Sakkoula et al Br. J Pharm. 122:793-795, 1997.|
|30||Scorrano et al. Dev. Cell, 2(1):55-67 (2002).|
|31||*||Verheul et al, J Pathol 202:5-13, 2004.|
|32||Wang et al. Genes Dev., 10(22):2859-2869 (1996).|
|33||Wennbo et al., "Transgenic Mice Overexpressing the Prolactin Gene Develop Dramatic Enlargement of the Prostate Gland", Endocrinology, vol. 138, No. 10, pp. 4410-4415, Apr. 1997.|
|34||Zhai et al. Eur. J. Biochem., 268(1):48-55 (2001).|
|35||Zhang et al., "A Novel Design of Targeted Endocrine and Cytokine Therapy for Breast Cancer", Clinical Cancer Research, vol. 8, pp. 1196-1205, Apr. 2002.|
|36||Zhang et al., "PowerBLAST: A New Network BLAST Application for Interactive or Automated Sequence Analysis and Annotation", Genome Research, vol. 7, pp. 649-656, 1997.|
|Citing Patent||Filing date||Publication date||Applicant||Title|
|US20110129873 *||Apr 30, 2009||Jun 2, 2011||Monsanto Technology Llc||Recombinant DNA Vectors for Expression of Human Prolactin Antagonists|
|WO2013034116A1 *||Sep 10, 2012||Mar 14, 2013||Tsinghua University||Vascular endothelial myostatin mutant that mutates at atp binding sites|
|International Classification||A61K38/27, C07K14/575, A61P35/00, A61K38/22, C07K5/10, C12N9/68, C07K14/47, A61K38/00, C07K14/78|
|Cooperative Classification||C12Y304/21007, C07K14/78, C07K2319/00, C07K14/47, C12N9/6435, C07K14/57554|
|European Classification||C12N9/64F21D, C07K14/575K, C07K14/47, C07K14/78|
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