WO1992013078A3 - Pasteurella haemolytica glycoprotease gene and the purified enzyme - Google Patents

Pasteurella haemolytica glycoprotease gene and the purified enzyme Download PDF

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Publication number
WO1992013078A3
WO1992013078A3 PCT/CA1992/000019 CA9200019W WO9213078A3 WO 1992013078 A3 WO1992013078 A3 WO 1992013078A3 CA 9200019 W CA9200019 W CA 9200019W WO 9213078 A3 WO9213078 A3 WO 9213078A3
Authority
WO
WIPO (PCT)
Prior art keywords
glycoprotease
purified enzyme
pasteurella haemolytica
gene
haemolytica glycoprotease
Prior art date
Application number
PCT/CA1992/000019
Other languages
French (fr)
Other versions
WO1992013078A2 (en
Inventor
Alan Mellors
Reggie Y C Lo
Khalid M Abdullah
Original Assignee
Univ Guelph
Alan Mellors
Reggie Y C Lo
Khalid M Abdullah
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Guelph, Alan Mellors, Reggie Y C Lo, Khalid M Abdullah filed Critical Univ Guelph
Priority to DK92902804T priority Critical patent/DK0567503T3/en
Priority to EP92902804A priority patent/EP0567503B1/en
Priority to US08/087,797 priority patent/US5543312A/en
Priority to DE69223502T priority patent/DE69223502T2/en
Publication of WO1992013078A2 publication Critical patent/WO1992013078A2/en
Publication of WO1992013078A3 publication Critical patent/WO1992013078A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea

Abstract

A purified DNA molecule comprises a DNA sequence of approximately 975 pb coding for a glycoprotease having a molecular weight of approximately 35.2 kD. The glycoprotease may be isolated from cultures of P.haemolytica. The glycoprotease is specific for cleaving O-glycosylated carbohydrate portions from O-glycoproteins. The glycoprotease has a major cleavage site in glycophorin A between Arg31-Asp32.
PCT/CA1992/000019 1991-01-15 1992-01-15 Pasteurella haemolytica glycoprotease gene and the purified enzyme WO1992013078A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DK92902804T DK0567503T3 (en) 1991-01-15 1992-01-15 Gene for Pasteurella haemolytica glycoprotease, as well as the purified enzyme.
EP92902804A EP0567503B1 (en) 1991-01-15 1992-01-15 Pasteurella haemolytica glycoprotease gene
US08/087,797 US5543312A (en) 1991-01-15 1992-01-15 Pastuerella haemolytica glycoprotease gene and the purified enzyme
DE69223502T DE69223502T2 (en) 1991-01-15 1992-01-15 GENES FOR THE GLYCOPROTEASE FROM PASTEURELLA HAEMOLYTICA

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB919100825A GB9100825D0 (en) 1991-01-15 1991-01-15 Pasteurella haemolytica glycoprotease gene and the purified enzyme
GB9100825.0 1991-01-15

Publications (2)

Publication Number Publication Date
WO1992013078A2 WO1992013078A2 (en) 1992-08-06
WO1992013078A3 true WO1992013078A3 (en) 1992-12-10

Family

ID=10688453

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1992/000019 WO1992013078A2 (en) 1991-01-15 1992-01-15 Pasteurella haemolytica glycoprotease gene and the purified enzyme

Country Status (8)

Country Link
US (1) US5543312A (en)
EP (1) EP0567503B1 (en)
AT (1) ATE161050T1 (en)
CA (1) CA2101781A1 (en)
DE (1) DE69223502T2 (en)
DK (1) DK0567503T3 (en)
GB (1) GB9100825D0 (en)
WO (1) WO1992013078A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837268A (en) * 1991-10-16 1998-11-17 University Of Saskatchewan GnRH-leukotoxin chimeras
US6793927B1 (en) 1993-12-06 2004-09-21 The United States Of America As Represented By The Secretary Of The Department Of Agriculture Construction of Pasteurella haemolytica vaccines
US5587305A (en) * 1993-12-06 1996-12-24 The United States Of America As Represented By The Department Of Agriculture Pasteurella haemolytica transformants
US6071724A (en) * 1996-12-13 2000-06-06 Eli Lilly And Company Streptococcus pneumoniaegene sequence era
GB9808423D0 (en) * 1998-04-22 1998-06-17 Glaxo Group Ltd Bacterial polypeptide family
US6740113B2 (en) * 1998-05-29 2004-05-25 Scimed Life Systems, Inc. Balloon expandable stent with a self-expanding portion
AU4392599A (en) * 1998-06-25 2000-01-10 Protegene Inc. Human glycoprotease-like proteins and dnas encoding these proteins
US6220934B1 (en) * 1998-07-23 2001-04-24 Micron Technology, Inc. Method for controlling pH during planarization and cleaning of microelectronic substrates
US6222026B1 (en) 1998-09-08 2001-04-24 Smithkline Beecham Corporation Gcp
US6368815B1 (en) * 1999-03-29 2002-04-09 Warner-Lambert Company Screening of molecules that inhibit human phosphodiesterase 4A produced by non-recombinant cell lines
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4906571A (en) * 1986-10-06 1990-03-06 University Of Guelph Cell surface modification using a novel glycoproteinase of pasteurella haemolytica

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4503035B1 (en) * 1978-11-24 1996-03-19 Hoffmann La Roche Protein purification process and product
US5082785A (en) * 1987-01-30 1992-01-21 Hoffmann-La Roche Inc. Biosynthesis of 2 keto-l-gulonic acid

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4906571A (en) * 1986-10-06 1990-03-06 University Of Guelph Cell surface modification using a novel glycoproteinase of pasteurella haemolytica

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Biochem. Soc. Transact., vol. 18, part 5, October 1990, (London, GB), K.M. ABDULLAH et al.: "Distribution of glycoprotease activity and the glycoprotease gene among serotypes of Pasteurella haemolytica", pages 901-903, see whole article (cited in the application) *
Infection and Immunity, vol. 50, no. 3, December 1985, (Washington, DC, US), R.Y.C. LO et al.: "Cloning and expression of the leukotoxin gene of Pasteurella haemolytica A1 in Escherichia coli K-12", pages 667-671, see whole document (cited in the application) *
Journal of Bacteriology, vol. 173, no. 18, September 1991, (Washington, DC, US), K.M. ABDULLAH et al.: "Cloning, nucleotide sequence and expression of the Pasteurella haemolytica A1 glycoprotease gene", pages 5597-5603, see whole document *

Also Published As

Publication number Publication date
CA2101781A1 (en) 1992-07-16
US5543312A (en) 1996-08-06
GB9100825D0 (en) 1991-02-27
DK0567503T3 (en) 1998-08-24
ATE161050T1 (en) 1997-12-15
EP0567503A1 (en) 1993-11-03
DE69223502T2 (en) 1998-07-16
DE69223502D1 (en) 1998-01-22
WO1992013078A2 (en) 1992-08-06
EP0567503B1 (en) 1997-12-10

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