WO1995003417A3 - Homologously recombinant slow growing mycobacteria and uses therefor - Google Patents

Homologously recombinant slow growing mycobacteria and uses therefor Download PDF

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Publication number
WO1995003417A3
WO1995003417A3 PCT/US1994/008267 US9408267W WO9503417A3 WO 1995003417 A3 WO1995003417 A3 WO 1995003417A3 US 9408267 W US9408267 W US 9408267W WO 9503417 A3 WO9503417 A3 WO 9503417A3
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WO
WIPO (PCT)
Prior art keywords
growing mycobacteria
homologously recombinant
slow
mycobacteria
uses therefor
Prior art date
Application number
PCT/US1994/008267
Other languages
French (fr)
Other versions
WO1995003417A2 (en
Inventor
Anna Aldovini
Richard A Young
Original Assignee
Whitehead Biomedical Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Whitehead Biomedical Inst filed Critical Whitehead Biomedical Inst
Priority to EP94922679A priority Critical patent/EP0710287A1/en
Publication of WO1995003417A2 publication Critical patent/WO1995003417A2/en
Publication of WO1995003417A3 publication Critical patent/WO1995003417A3/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/32—Mycobacterium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

A method of transforming slow-growing mycobacteria, such as M. bovis BCG, M. leprae, M. tuberculosis, M. avium, M. intracellulare and M. africanum; a method of manipulating genomic DNA of slow-growing mycobacteria through homologous recombination; a method of producing homologously recombinant (HR) slow-growing mycobacteria in which heterologous DNA is integrated into the genomic DNA at a homologous locus; homologously recombinant (HR) slow-growing mycobacteria having heterologous DNA integrated into their genomic DNA at a homologous locus; and mycobacteria DNA useful as a genetic marker.
PCT/US1994/008267 1993-07-22 1994-07-22 Homologously recombinant slow growing mycobacteria and uses therefor WO1995003417A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP94922679A EP0710287A1 (en) 1993-07-22 1994-07-22 Homologously recombinant slow growing mycobacteria and uses therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/095,734 US5807723A (en) 1987-03-02 1993-07-22 Homologously recombinant slow growing mycobacteria and uses therefor
US08/095,734 1993-07-22

Publications (2)

Publication Number Publication Date
WO1995003417A2 WO1995003417A2 (en) 1995-02-02
WO1995003417A3 true WO1995003417A3 (en) 1995-04-06

Family

ID=22253350

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/008267 WO1995003417A2 (en) 1993-07-22 1994-07-22 Homologously recombinant slow growing mycobacteria and uses therefor

Country Status (3)

Country Link
US (4) US5807723A (en)
EP (1) EP0710287A1 (en)
WO (1) WO1995003417A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807723A (en) 1987-03-02 1998-09-15 Whitehead Institute For Biomedical Research Homologously recombinant slow growing mycobacteria and uses therefor
US5843664A (en) * 1996-06-11 1998-12-01 Institut Pasteur Method of selection of allelic exchange mutants
JP2001518781A (en) * 1997-03-28 2001-10-16 サイタクラナル、ファーマスーティクス、インク Mycobacterium recombinant vaccine
WO1998056931A1 (en) * 1997-06-11 1998-12-17 Institut Pasteur Attenuated recombinant mycobacteria useful as immunogens or as vaccine components
DK1036198T3 (en) 1997-12-08 2013-01-02 California Inst Of Techn Method for Preparation of Polynucleotide and Polypeptide Sequences
US20030124685A1 (en) * 1999-02-01 2003-07-03 Yoko Kuwabara Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing l-arginine
US6255086B1 (en) 1999-02-01 2001-07-03 Ajinomoto Co., Inc. Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
AU5559300A (en) * 1999-07-06 2001-01-22 Institut Pasteur Method of making and identifying attenuated microorganisms, compositions utilizing the sequences responsible for attenuation, and preparations containing attenuated microorganisms
AU2001230912A1 (en) 2000-01-11 2001-07-24 The Board Of Regents Of The University Of Nebraska Identification of virulence determinants
US6924118B2 (en) * 2000-04-17 2005-08-02 The Regents Of The University Of California Recombinant intracellular pathogen immunogenic compositions and methods for use
US6471967B1 (en) 2000-04-17 2002-10-29 The Regents Of The University Of California Recombinant intracellular pathogen vaccines and methods for use
KR20030005836A (en) * 2001-07-10 2003-01-23 현대자동차주식회사 Small parts manufacturing method for vehicle
TR200501010T2 (en) * 2002-09-26 2005-05-23 Kaya K�Ksalan Orhan Molecular size markers for the detection of mycobacterial species
EP1684798A4 (en) * 2003-10-16 2007-12-05 Univ California Recombinant intracellular pathogen immunogenic compositions and methods for use
US8383132B2 (en) * 2003-10-16 2013-02-26 The Regents Of The University Of California Immunostimulatory recombinant intracellular pathogen immunogenic compositions and methods of use
US8168421B2 (en) * 2005-12-09 2012-05-01 University Of Georgia Research Foundation, Inc. Microbial vaccine and vaccine vector

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990000594A2 (en) * 1988-07-07 1990-01-25 Whitehead Institute For Biomedical Research Recombinant mycobacterial expression vehicles and uses therefor
WO1990015873A1 (en) * 1989-06-19 1990-12-27 Whitehead Institute For Biomedical Research Vector-mediated genomic insertion and expression of dna in bcg

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59205983A (en) * 1983-04-28 1984-11-21 ジエネツクス・コ−ポレイシヨン Development of different kind gene by procaryotic microorganism
GB8314645D0 (en) * 1983-05-26 1983-06-29 Wellcome Found Bivalent vaccines
US4906742A (en) * 1986-07-31 1990-03-06 Whitehead Institute For Biomedical Research Encoding antigens of M. Leprae
US5807723A (en) 1987-03-02 1998-09-15 Whitehead Institute For Biomedical Research Homologously recombinant slow growing mycobacteria and uses therefor
EP0347425B1 (en) * 1987-03-02 1995-12-27 Whitehead Institute For Biomedical Research Recombinant mycobacterial vaccine
US4952500A (en) * 1988-02-01 1990-08-28 University Of Georgia Research Foundation, Inc. Cloning systems for Rhodococcus and related bacteria
US4910140A (en) * 1988-04-18 1990-03-20 Bio-Rad Laboratories, Inc. Electroporation of prokaryotic cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990000594A2 (en) * 1988-07-07 1990-01-25 Whitehead Institute For Biomedical Research Recombinant mycobacterial expression vehicles and uses therefor
WO1990015873A1 (en) * 1989-06-19 1990-12-27 Whitehead Institute For Biomedical Research Vector-mediated genomic insertion and expression of dna in bcg

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANNA ALDOVINI ET AL.: "The uraA locus and homologous recombination in Mycobacterium bovis", JOURNAL OF BACTERIOLOGY, vol. 175, no. 22, November 1993 (1993-11-01), pages 7282 - 7289 *
J. HERMANS ET AL.: "Transformation of Mycobacterium aurum by electroporation: the use of glycine, lysozyme and isonicotinic acid hydrazide in enhancing transformation efficiency", FEMS MICROBIOLOGY LETTERS, vol. 72, 1990, pages 221 - 224 *
ROBERT N. HUSSON ET AL.: "Gene replacement and expression of foreign DNA in Mycobacteria", JOURNAL OF BACTERIOLOGY, vol. 172, no. 2, February 1990 (1990-02-01), pages 519 - 524 *
WILLIAM R. JACOBS, JR. ET AL.: "Genetic systems for Mycobacteria", METHODS IN ENZYMOLOGY, vol. 204, 1991, pages 537 - 555 *

Also Published As

Publication number Publication date
US6355486B1 (en) 2002-03-12
US6022745A (en) 2000-02-08
US5807723A (en) 1998-09-15
WO1995003417A2 (en) 1995-02-02
US5866403A (en) 1999-02-02
EP0710287A1 (en) 1996-05-08

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