WO1995004162A3 - Dna sequencing enzymes - Google Patents

Dna sequencing enzymes Download PDF

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Publication number
WO1995004162A3
WO1995004162A3 PCT/US1994/008610 US9408610W WO9504162A3 WO 1995004162 A3 WO1995004162 A3 WO 1995004162A3 US 9408610 W US9408610 W US 9408610W WO 9504162 A3 WO9504162 A3 WO 9504162A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
polymerases
dna sequencing
family
sequencing
Prior art date
Application number
PCT/US1994/008610
Other languages
French (fr)
Other versions
WO1995004162A2 (en
Inventor
Myron F Goodman
Linda J Reha-Krantz
Original Assignee
Univ Southern California
Univ Alberta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to PL94312836A priority Critical patent/PL312836A1/en
Priority to JP7506010A priority patent/JPH09509301A/en
Priority to SK141-96A priority patent/SK14196A3/en
Priority to AU74088/94A priority patent/AU699498B2/en
Priority to NZ269727A priority patent/NZ269727A/en
Priority to KR1019960700551A priority patent/KR960704067A/en
Priority to BR9407403A priority patent/BR9407403A/en
Priority to EP94924079A priority patent/EP0713535A1/en
Application filed by Univ Southern California, Univ Alberta filed Critical Univ Southern California
Publication of WO1995004162A2 publication Critical patent/WO1995004162A2/en
Publication of WO1995004162A3 publication Critical patent/WO1995004162A3/en
Priority to BG100188A priority patent/BG100188A/en
Priority to NO960408A priority patent/NO960408L/en
Priority to FI960469A priority patent/FI960469A/en
Priority to LVP-96-23A priority patent/LV11486B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

There are provided variant family B DNA polymerases having no 3'→5' exonuclease activity. These variant polymerases have utility as DNA sequencing polymerases. Methods for DNA sequencing with family B DNA polymerases and chain-terminating nucleotides not previously used for sequencing have been developed. The methods disclosed involve the use of family B DNA polymerases not known heretofore to have utility in DNA sequencing, such as variant or wild type forms of phage T4 DNA polymerase or Escherichia coli DNA polymerase II, with novel combinations of deoxynucleotides and chain-terminating nucleotides.
PCT/US1994/008610 1993-08-02 1994-08-01 Dna sequencing enzymes WO1995004162A2 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
BR9407403A BR9407403A (en) 1993-08-02 1994-08-01 DNA polymerase and method for DNA sequence and method for identification and isolation of DNA polymerase
SK141-96A SK14196A3 (en) 1993-08-02 1994-08-01 Enzymes sequencing dna
AU74088/94A AU699498B2 (en) 1993-08-02 1994-08-01 DNA sequencing enzymes
NZ269727A NZ269727A (en) 1993-08-02 1994-08-01 A group of dna polymerase which have no 3'-5' exonuclease activity
KR1019960700551A KR960704067A (en) 1993-08-02 1994-08-01 DNA Sequencing Enzymes
PL94312836A PL312836A1 (en) 1993-08-02 1994-08-01 Variations of family b of dna polymerase used for dna sequencing
EP94924079A EP0713535A1 (en) 1993-08-02 1994-08-01 Dna sequencing enzymes
JP7506010A JPH09509301A (en) 1993-08-02 1994-08-01 Enzyme for DNA sequencing
BG100188A BG100188A (en) 1993-08-02 1995-12-04 Dna sequence enzymes
NO960408A NO960408L (en) 1993-08-02 1996-01-31 DNA sequencing enzymes
FI960469A FI960469A (en) 1993-08-02 1996-02-01 DNA sequencing enzymes
LVP-96-23A LV11486B (en) 1993-08-02 1996-02-14 Dna sequencing enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/101,593 US5547859A (en) 1993-08-02 1993-08-02 Chain-terminating nucleotides for DNA sequencing methods
US08/101,593 1993-08-02

Publications (2)

Publication Number Publication Date
WO1995004162A2 WO1995004162A2 (en) 1995-02-09
WO1995004162A3 true WO1995004162A3 (en) 1995-06-01

Family

ID=22285458

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/008610 WO1995004162A2 (en) 1993-08-02 1994-08-01 Dna sequencing enzymes

Country Status (18)

Country Link
US (3) US5547859A (en)
EP (1) EP0713535A1 (en)
JP (1) JPH09509301A (en)
KR (1) KR960704067A (en)
CN (1) CN1132529A (en)
AU (1) AU699498B2 (en)
BG (1) BG100188A (en)
BR (1) BR9407403A (en)
CA (1) CA2168471A1 (en)
CZ (1) CZ30696A3 (en)
FI (1) FI960469A (en)
HU (1) HUT75841A (en)
LV (1) LV11486B (en)
NO (1) NO960408L (en)
NZ (1) NZ269727A (en)
PL (1) PL312836A1 (en)
SK (1) SK14196A3 (en)
WO (1) WO1995004162A2 (en)

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US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
EP0910664A1 (en) * 1996-04-15 1999-04-28 University Of Southern California Synthesis of fluorophore-labeled dna
US5827716A (en) * 1996-07-30 1998-10-27 Amersham Life Science, Inc. Modified Pol-II type DNA polymerases
US6291164B1 (en) * 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
EP1082458A1 (en) * 1998-05-01 2001-03-14 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1226255B1 (en) * 1999-10-29 2006-03-29 Stratagene California Compositions and methods utilizing dna polymerases
US20040009486A1 (en) 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (en) * 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2248911A1 (en) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
WO2006007207A2 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
EP2071927A2 (en) 2006-09-28 2009-06-24 Illumina, Inc. Compositions and methods for nucleotide sequencing
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
EP2725107B1 (en) 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified ddNTPs and nucleic acid comprising inosine with reversible terminators
EP2940029B1 (en) 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
US7811810B2 (en) 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7767441B2 (en) * 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
DK2245199T3 (en) 2008-02-01 2014-02-03 Gen Hospital Corp APPLICATION OF MICROVESCIES IN DIAGNOSTICATION, PROGRAMMING AND TREATMENT OF MEDICAL DISEASES AND CONDITIONS
US20130029339A1 (en) 2009-09-09 2013-01-31 The General Hospital Corporation Use of microvesicles in analyzing kras mutations
EP3461912B1 (en) 2009-09-09 2022-07-13 The General Hospital Corporation Use of microvesicles in analyzing nucleic acid profiles
WO2012031008A2 (en) 2010-08-31 2012-03-08 The General Hospital Corporation Cancer-related biological materials in microvesicles
WO2012064993A1 (en) 2010-11-10 2012-05-18 Exosome Diagnosties, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
EP2903597B1 (en) 2012-10-03 2019-12-18 Exosome Diagnostics Inc. Use of microvesicles in diagnosis, prognosis, and treatment of medical diseases and conditions
DK2970356T3 (en) 2013-03-15 2018-08-27 Illumina Cambridge Ltd Modified nucleosides or nucleotides
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
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EP0516245A1 (en) * 1987-01-14 1992-12-02 President And Fellows Of Harvard College DNA Sequencing
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WO1991016446A1 (en) * 1989-03-24 1991-10-31 Consejo Superior Investigaciones Cientificas In vitro dna synthesis reactions using phi 29 dna polymerase and a dna fragment encoding said polymerase
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Also Published As

Publication number Publication date
FI960469A (en) 1996-03-27
AU7408894A (en) 1995-02-28
CN1132529A (en) 1996-10-02
PL312836A1 (en) 1996-05-13
LV11486A (en) 1996-08-20
CA2168471A1 (en) 1995-02-09
NZ269727A (en) 1997-11-24
AU699498B2 (en) 1998-12-03
WO1995004162A2 (en) 1995-02-09
BR9407403A (en) 1996-11-05
JPH09509301A (en) 1997-09-22
HU9600235D0 (en) 1996-03-28
BG100188A (en) 1996-12-31
SK14196A3 (en) 1996-06-05
LV11486B (en) 1997-02-20
US5928919A (en) 1999-07-27
US5660980A (en) 1997-08-26
CZ30696A3 (en) 1996-06-12
NO960408D0 (en) 1996-01-31
FI960469A0 (en) 1996-02-01
NO960408L (en) 1996-03-27
EP0713535A1 (en) 1996-05-29
KR960704067A (en) 1996-08-31
HUT75841A (en) 1997-05-28
US5547859A (en) 1996-08-20

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