WO1995009051A1 - A mechanical triturator for biological material - Google Patents

A mechanical triturator for biological material Download PDF

Info

Publication number
WO1995009051A1
WO1995009051A1 PCT/EP1994/003202 EP9403202W WO9509051A1 WO 1995009051 A1 WO1995009051 A1 WO 1995009051A1 EP 9403202 W EP9403202 W EP 9403202W WO 9509051 A1 WO9509051 A1 WO 9509051A1
Authority
WO
WIPO (PCT)
Prior art keywords
plate
chamber
cutting member
rotation
input portion
Prior art date
Application number
PCT/EP1994/003202
Other languages
French (fr)
Inventor
Gianmarco Roggero
Original Assignee
Consul T.S. S.R.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consul T.S. S.R.L. filed Critical Consul T.S. S.R.L.
Priority to AU78098/94A priority Critical patent/AU676386B2/en
Priority to US08/615,282 priority patent/US5731199A/en
Priority to CA002171117A priority patent/CA2171117C/en
Priority to DE69412272T priority patent/DE69412272T2/en
Priority to DK94928814T priority patent/DK0720513T3/en
Priority to EP94928814A priority patent/EP0720513B1/en
Priority to JP51010795A priority patent/JP3604141B2/en
Publication of WO1995009051A1 publication Critical patent/WO1995009051A1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
    • B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
    • B02C18/08—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers
    • B02C18/10—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers with drive arranged above container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
    • B02C18/30—Mincing machines with perforated discs and feeding worms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
    • B02C18/30—Mincing machines with perforated discs and feeding worms
    • B02C18/36—Knives or perforated discs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting

Definitions

  • the present invention relates to a mechanical triturator or grinder for biological materials such as animal or vegetable tissues and the like, which is suitable for laboratory and/or industrial use to produce separate cells or cell nuclei, for example, for biopsy or DNA analysis.
  • the object of the present invention is to provide a mechanical device which avoids lengthy and tedious mechanical chopping operations carried out with scalpels or the like and enzymatic cell-separation treatments.
  • a mechanical triturator comprising:
  • a cutting member in the form of a foraminous plate 6 which is disposed transversely in the chamber 4 so as to define an input portion 12 for the supply of the material to be triturated and a portion 14 for collecting the triturated material, and which has a plurality of blades extending from the general plane of the plate 6 into the input portion 12,
  • a rotor member 16 mounted for rotation in the chamber 4 and having a grinding member 20 which is fixed to the rotor 16 and is disposed in the input portion 12, and cooperates with the cutting member 6 in order, as a result of its rotation, to supply the biological material into contact with the blades and to cause the trituration thereof.
  • Figure 1 is a schematic, axial section of a triturator according to the invention
  • Figure 2 is an exploded perspective view of the triturator of Figure 1
  • FIGS 3, 4 and 5 show schematically the sequence of use of the triturator according to the invention.
  • a triturator comprises a cylindrical housing 2 of plastics material defining a chamber 4.
  • a cutting member constituted by a perforated plate 6 or disc is disposed transversely in the chamber 4.
  • the plate 6 may be produced by the punching and stretching of a stainless steel plate.
  • the perforations in the plate have preferably dimensions of between 20 and 100 ⁇ m and the edges of each microperforation constitute trituration blades.
  • the microperforations can be produced by punches with any profile and preferably square or hexagonal profiles so that the perforated plate 6 preferably has from four to six blades surrounding each hole.
  • a peripheral edge of the perforated plate bears against a shoulder 8 of the cylindrical housing 2 and the perforated plate is fixed to the latter by means of a cylindrical bush 10 coupled with the cylindrical internal wall of the housing 2.
  • the perforated plate 6 divides the chamber 4 of the housing 2 into an input portion 12 for the supply of the material to be triturated and a portion 14 for collecting the triturated material.
  • the blades of the perforated plate 6 are oriented towards the input portion 12.
  • a rotor member 16 disposed in the chamber 4 of the housing 2 comprises a shaft 18 to which a grinding member or screw 20 is fixed; the screw 20 being disposed in the input portion 12 of the chamber 4 and cooperating with the perforated plate 6 in order, as a result of its rotation at high speed, to supply the biological material into contact with the blades of the perforated plate 6 so as to cause the trituration of the material.
  • the shaft 18 is supported for rotation by a plain bearing 22 integral with the bush 10.
  • the bearing 22 is connected to the side walls of the bush 10 by means of two or more spokes 24 between which holes are defined for the introduction of the material to be triturated.
  • the upper portion of the cylindrical housing 2 is closed by a cover 26 having a central hole 28 through which the shaft 18 extends.
  • the rotor member 16 also comprises a sweeping blade member or rotary screw 30 having one or more flat blades.
  • the sweeping blade member 30 is fixed to an end of the shaft 18 which projects into the collection portion 14 through a hole 32 in the perforated plate 6.
  • the blade member 30 is adjacent and parallel to the lower surface of the plate in the collection portion and, in operation, creates an upwards flow of liquid through the holes in the plate and thus cleans the holes.
  • the liquid which exerts this cleaning action may be contained in the biological sample since the latter is generally constituted by a suspension of tissue or aggregated cells in a liquid.
  • the shaft 18 has a shank 34 at its upper end for connecting it to the shaft of a motor, for example, of the turbine type, for rotating the rotor member 16.
  • the base of the cylindrical housing 2 is constituted by a disc 36 which is connected to the rest of the housing by means of a weakened portion 38.
  • the disc 36 is coupled with a seat 40 in a cup-like body 42 which is intended to collect the triturated material.
  • the housing 2 is fixed to the cup-like body 42 by the plug-in or snap- coupling of the disc 36 with the seat 40 (see Figures 3 and 4) .
  • the triturated material is collected by the removal of the cylindrical housing 2 from the base 42.
  • the weakened portion 38 which connects the base disc 36 to the housing 2 is torn. This ensures that the device can be used only once, to prevent problems of contamination of the material processed.
  • All the components of the triturator 1 except the perforated plate 6 may be made of sterilizable plastics materials suitable for use in the medical field.

Abstract

A mechanical triturator for biological material comprises a cylindrical housing (2) defining a chamber (4) in which a cutting member (6) constituted by a perforated plate is disposed. A rotor member (16), mounted for rotation in the chamber (4), has a screw (20) which cooperates with the cutting member (6) in order, as a result of its rotation, to supply the biological material into contact with the cutting member (6) and to cause the trituration thereof.

Description

A mechanical triturator for biological material
The present invention relates to a mechanical triturator or grinder for biological materials such as animal or vegetable tissues and the like, which is suitable for laboratory and/or industrial use to produce separate cells or cell nuclei, for example, for biopsy or DNA analysis.
The object of the present invention is to provide a mechanical device which avoids lengthy and tedious mechanical chopping operations carried out with scalpels or the like and enzymatic cell-separation treatments.
According to the present invention, this object is achieved by a mechanical triturator comprising:
a cylindrical housing 2 defining a chamber 4,
a cutting member in the form of a foraminous plate 6 which is disposed transversely in the chamber 4 so as to define an input portion 12 for the supply of the material to be triturated and a portion 14 for collecting the triturated material, and which has a plurality of blades extending from the general plane of the plate 6 into the input portion 12,
a rotor member 16 mounted for rotation in the chamber 4 and having a grinding member 20 which is fixed to the rotor 16 and is disposed in the input portion 12, and cooperates with the cutting member 6 in order, as a result of its rotation, to supply the biological material into contact with the blades and to cause the trituration thereof.
Further characteristics and advantages of the present invention will become clear in the course of the detailed description which follows, given purely by way of non- limiting example, with reference to the appended drawings, in which:
Figure 1 is a schematic, axial section of a triturator according to the invention,
Figure 2 is an exploded perspective view of the triturator of Figure 1, and
Figures 3, 4 and 5 show schematically the sequence of use of the triturator according to the invention.
With reference to Figures 1 and 2, a triturator, indicated 1, comprises a cylindrical housing 2 of plastics material defining a chamber 4.
A cutting member constituted by a perforated plate 6 or disc is disposed transversely in the chamber 4. The plate 6 may be produced by the punching and stretching of a stainless steel plate. The perforations in the plate have preferably dimensions of between 20 and 100 μm and the edges of each microperforation constitute trituration blades. The microperforations can be produced by punches with any profile and preferably square or hexagonal profiles so that the perforated plate 6 preferably has from four to six blades surrounding each hole.
A peripheral edge of the perforated plate bears against a shoulder 8 of the cylindrical housing 2 and the perforated plate is fixed to the latter by means of a cylindrical bush 10 coupled with the cylindrical internal wall of the housing 2. The perforated plate 6 divides the chamber 4 of the housing 2 into an input portion 12 for the supply of the material to be triturated and a portion 14 for collecting the triturated material. The blades of the perforated plate 6 are oriented towards the input portion 12.
A rotor member 16 disposed in the chamber 4 of the housing 2 comprises a shaft 18 to which a grinding member or screw 20 is fixed; the screw 20 being disposed in the input portion 12 of the chamber 4 and cooperating with the perforated plate 6 in order, as a result of its rotation at high speed, to supply the biological material into contact with the blades of the perforated plate 6 so as to cause the trituration of the material. The shaft 18 is supported for rotation by a plain bearing 22 integral with the bush 10. The bearing 22 is connected to the side walls of the bush 10 by means of two or more spokes 24 between which holes are defined for the introduction of the material to be triturated.
The upper portion of the cylindrical housing 2 is closed by a cover 26 having a central hole 28 through which the shaft 18 extends.
The rotor member 16 also comprises a sweeping blade member or rotary screw 30 having one or more flat blades. The sweeping blade member 30 is fixed to an end of the shaft 18 which projects into the collection portion 14 through a hole 32 in the perforated plate 6. The blade member 30 is adjacent and parallel to the lower surface of the plate in the collection portion and, in operation, creates an upwards flow of liquid through the holes in the plate and thus cleans the holes. The liquid which exerts this cleaning action may be contained in the biological sample since the latter is generally constituted by a suspension of tissue or aggregated cells in a liquid.
The shaft 18 has a shank 34 at its upper end for connecting it to the shaft of a motor, for example, of the turbine type, for rotating the rotor member 16.
The base of the cylindrical housing 2 is constituted by a disc 36 which is connected to the rest of the housing by means of a weakened portion 38. The disc 36 is coupled with a seat 40 in a cup-like body 42 which is intended to collect the triturated material.
With reference to Figures 3, 4 and 5, the housing 2 is fixed to the cup-like body 42 by the plug-in or snap- coupling of the disc 36 with the seat 40 (see Figures 3 and 4) . Upon completion of the trituration of the material, the triturated material is collected by the removal of the cylindrical housing 2 from the base 42. During this operation, the weakened portion 38 which connects the base disc 36 to the housing 2 is torn. This ensures that the device can be used only once, to prevent problems of contamination of the material processed.
All the components of the triturator 1 except the perforated plate 6 may be made of sterilizable plastics materials suitable for use in the medical field.
The simplicity and ease of assembly of the various components of the device enable the cost to be kept to an acceptable level for disposable devices.

Claims

1. A mechanical triturator for biological materials such as animal or vegetable tissues and the like, characterised in that it comprises:
a cylindrical housing (2) defining a chamber (4),
a cutting member in the form of a foraminous plate (6) which is disposed transversely in the chamber (4) so as to define an input portion (12) for the supply of the material to be triturated and a portion (14) for collecting the triturated material, and which has a plurality of blades extending from the general plane of the plate (6) into the input portion (12) ,
a rotor member (16) mounted for rotation in the chamber (4) and having a grinding member (20) which is fixed to the rotor (16) and is disposed in the input portion (12) , and cooperates with the cutting member (6) in order, as a result of its rotation, to supply the biological material into contact with the blades and to cause the trituration thereof.
2. A device according to Claim 1, characterised in that the rotary member (16) comprises a sweeping blade member (30) which is fixed for rotation with the rotor (16) , and is disposed adjacent and parallel to the lower surface of the plate (6) in the collection portion (14) , said sweeping blade member (30) being suitable for preventing the biological material from obstructing the holes during trituration.
3. A device according to Claim 1 or Claim 2, characterised in that the plate (6) constituting the cutting member is a micro-stretched foraminous metal plate with holes having dimensions of between 20 and 100 urn.
4. A device according to Claim 3, characterised in that the foraminous plate (6) constituting the cutting member has from four to six blades surrounding each hole.
5. A device according to any one of the preceding claims, characterised in that the housing comprises a cylindrical body having an open end with a cover (26) for closing the input portion (12) and a base (36) which can be separated from the housing (2) by the tearing of a weakened portion (38) , the base (36) being connectible to a cup-like container (42) for collecting the triturated material.
PCT/EP1994/003202 1993-09-28 1994-09-26 A mechanical triturator for biological material WO1995009051A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU78098/94A AU676386B2 (en) 1993-09-28 1994-09-26 A mechanical triturator for biological material
US08/615,282 US5731199A (en) 1993-09-28 1994-09-26 Mechanical triturator for biological material
CA002171117A CA2171117C (en) 1993-09-28 1994-09-26 A mechanical triturator for biological material
DE69412272T DE69412272T2 (en) 1993-09-28 1994-09-26 MECHANICAL CRUSHING DEVICE FOR BIOLOGICAL MATERIAL
DK94928814T DK0720513T3 (en) 1993-09-28 1994-09-26 Apparatus for mechanical comminution of a biological material
EP94928814A EP0720513B1 (en) 1993-09-28 1994-09-26 A mechanical triturator for biological material
JP51010795A JP3604141B2 (en) 1993-09-28 1994-09-26 Mechanical triturator for biological materials

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITTO93A000706 1993-09-28
ITTO930706A IT1260682B (en) 1993-09-28 1993-09-28 MECHANICAL SHREDDER DEVICE OF BIOLOGICAL MATERIAL

Publications (1)

Publication Number Publication Date
WO1995009051A1 true WO1995009051A1 (en) 1995-04-06

Family

ID=11411757

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1994/003202 WO1995009051A1 (en) 1993-09-28 1994-09-26 A mechanical triturator for biological material

Country Status (11)

Country Link
US (1) US5731199A (en)
EP (1) EP0720513B1 (en)
JP (1) JP3604141B2 (en)
AT (1) ATE169244T1 (en)
AU (1) AU676386B2 (en)
CA (1) CA2171117C (en)
DE (1) DE69412272T2 (en)
DK (1) DK0720513T3 (en)
ES (1) ES2121230T3 (en)
IT (1) IT1260682B (en)
WO (1) WO1995009051A1 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997024933A1 (en) * 1996-01-05 1997-07-17 Grow Green Pty. Ltd. Protein feedstock, compositions and methods and apparatus for the production thereof
WO2000053128A1 (en) * 1999-03-10 2000-09-14 Clokie Cameron M L Oral craniofacial bone mill
CN104450509A (en) * 2014-09-29 2015-03-25 江南大学 Spirulina cell-wall breaking shearing device
WO2019012231A1 (en) 2017-07-12 2019-01-17 Stemcis Tissue mechanical fragmentation device intended for the preparation of a composition of isolated cells, and corresponding method
WO2019145029A1 (en) * 2018-01-24 2019-08-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Device and method for dissociating tissue
EP3896145A1 (en) * 2012-09-06 2021-10-20 GID BIO, Inc. Tissue processing apparatus and method for processing adipose tissue
US11717602B2 (en) 2016-08-30 2023-08-08 Lifecell Corporation Systems and methods for medical device control

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6398402B1 (en) * 1998-02-11 2002-06-04 Chris Thomas Disposable disruptor agitator tool having a bladed rotor disposed in a stator
IT1320860B1 (en) * 2000-12-13 2003-12-10 Consul T S S R L EQUIPMENT FOR THE DISGREGATION TREATMENT OF BIOLOGICAL SAMPLES AND DISGREGATOR DEVICE FOR USE
NZ518432A (en) * 2002-04-16 2004-09-24 Agres Ltd A homogeniser and method of cleaning same.
US20040173697A1 (en) * 2003-03-07 2004-09-09 Emerson Electric Co. Food waste reduction mechanism for disposer
TWI255738B (en) * 2003-12-31 2006-06-01 Ind Tech Res Inst Tissue pulverizer
DE102004009708B3 (en) * 2004-02-27 2005-09-01 Ika - Werke Gmbh & Co. Kg dispersing
ITTO20040743A1 (en) * 2004-10-27 2005-01-27 Gianmarco Roggero DISPOSABLE HOMOGENIZING DEVICE, AND ITS USE FOR THE PREPARATION OF HOMOGENIZED SAMPLES
DE102005034629B4 (en) * 2005-07-19 2007-09-13 Dr. Hielscher Gmbh Device and method for the mechanical disruption of cells
DE502005002844D1 (en) * 2005-09-16 2008-03-27 Prionics Ag Apparatus for shredding biological sample material
EP1975593B1 (en) * 2006-01-20 2019-10-16 Mizuho Medy Co., Ltd. Sample trituration vessel and tool
DE102008062570B3 (en) * 2008-12-16 2010-01-21 Ika-Werke Gmbh & Co. Kg Dispersing device, has roller bearing arranged in axial conveying direction at back side to dispersing region that is formed from rotor and stator, and outlet port for treating medium and arranged at back side of roller bearing
CA3056049A1 (en) 2010-06-01 2011-12-08 Auxocell Laboratories, Inc. Method of culturing mesenchymal stem cells and uses thereof in therapy
ES2725564T3 (en) 2011-11-08 2019-09-24 Auxocell Laboratories Inc Systems and methods of cellular processing
USD748462S1 (en) 2014-08-11 2016-02-02 Auxocell Laboratories, Inc. Centrifuge clip
US9993748B2 (en) 2014-08-11 2018-06-12 Auxocell Laboratories, Inc. Centrifuge clip and method
CN106604985B (en) 2014-09-04 2019-05-14 贝克顿·迪金森公司 Device and method for dissociating biological tissue samples
MX2017007932A (en) * 2014-12-15 2017-12-14 Human Brain Wave S R L Disgregating device of biological material and corresponding manufacturing method and method for the preparation of cell suspensions and tissue micrografts.
ES2647458B1 (en) * 2016-06-21 2018-09-28 Jose Miguel CASANOVA ROSELL System and method for capillary regeneration based on autologous stem cell micrografts and their use.
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WO2018222376A1 (en) 2017-06-01 2018-12-06 Becton, Dickinson And Company Devices for dissociating a biological tissue sample and methods of use thereof
CN108579973A (en) * 2017-12-13 2018-09-28 贵州金银药业有限公司 The quick mashing device of Chinese medicine
US20200072712A1 (en) * 2018-08-29 2020-03-05 Predictive Technology Group, Inc. Tissue mincers, related systems, and related methods
WO2020101622A1 (en) * 2018-11-12 2020-05-22 T-Bi̇yoteknoloji̇ Laboratuvar Esteti̇k Medi̇kal Kozmeti̇k San Ti̇c Ltd Şti̇ Blade - filter kit developed to be used for fat transfer
US11241661B2 (en) * 2019-04-15 2022-02-08 M. Technique Co., Ltd. Stirrer
US11033295B2 (en) 2019-05-06 2021-06-15 Tissuemill Technologies Llc Atraumatically formed tissue composition, devices and methods of preparation and treatment
IT201900006854A1 (en) 2019-05-15 2020-11-15 Fidia Farm Spa Device for disintegrating biological material and related method of disintegration and cell preparations
IT201900014106A1 (en) * 2019-08-06 2019-11-06 Armando Roggero Disruptor device and polymeric dissociator of solid biological materials for medical and regenerative surgical use
US20220349784A1 (en) * 2020-01-20 2022-11-03 Genetrack Biolabs Inc. Methods and devices for mincing biological tissue
GB2587257B (en) 2020-03-09 2021-09-15 Alma Lasers Ltd Lipoaspirate processing
CN115916411A (en) * 2020-04-06 2023-04-04 迈阿密大学 Apparatus and method for tissue processing
US20220025316A1 (en) 2020-07-22 2022-01-27 Prim Sigma Technologies, Inc. Trituration devices for tissue disaggregation
US11491490B1 (en) * 2021-05-27 2022-11-08 Biogreen 360, Inc. Organic waste management system

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1480969A (en) * 1923-07-27 1924-01-15 Nutriment Ltd Disintegrating solid substances
US2801665A (en) 1956-09-28 1957-08-06 Robert Friess K G Apparatus for comminuting meat and vegetables
FR1309224A (en) * 1961-08-17 1962-11-16 Fleischereimaschinen Ges M B H Machine for fractionating meat or the like
DE2601953A1 (en) * 1975-07-30 1977-02-17 Shinnosuke Funakubo CUTTING PLATE FOR MEAT MINCER AND METHOD OF MANUFACTURING THEREOF
EP0191010A1 (en) * 1985-02-04 1986-08-13 VOGELBUSCH GESELLSCHAFT m.b.H. Apparatus for disintegrating and extruding pasty stock

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3666187A (en) * 1970-05-13 1972-05-30 Us Health Education & Welfare Laboratory homogenizer
US5114858A (en) * 1990-06-26 1992-05-19 E. I. Du Pont De Nemours And Company Cellular component extraction process in a disposable filtration vessel

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1480969A (en) * 1923-07-27 1924-01-15 Nutriment Ltd Disintegrating solid substances
US2801665A (en) 1956-09-28 1957-08-06 Robert Friess K G Apparatus for comminuting meat and vegetables
FR1309224A (en) * 1961-08-17 1962-11-16 Fleischereimaschinen Ges M B H Machine for fractionating meat or the like
DE2601953A1 (en) * 1975-07-30 1977-02-17 Shinnosuke Funakubo CUTTING PLATE FOR MEAT MINCER AND METHOD OF MANUFACTURING THEREOF
EP0191010A1 (en) * 1985-02-04 1986-08-13 VOGELBUSCH GESELLSCHAFT m.b.H. Apparatus for disintegrating and extruding pasty stock

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997024933A1 (en) * 1996-01-05 1997-07-17 Grow Green Pty. Ltd. Protein feedstock, compositions and methods and apparatus for the production thereof
WO2000053128A1 (en) * 1999-03-10 2000-09-14 Clokie Cameron M L Oral craniofacial bone mill
EP3896145A1 (en) * 2012-09-06 2021-10-20 GID BIO, Inc. Tissue processing apparatus and method for processing adipose tissue
US11261418B2 (en) 2012-09-06 2022-03-01 The Gid Group, Inc. Tissue processing apparatus and method for processing adipose tissue
CN104450509A (en) * 2014-09-29 2015-03-25 江南大学 Spirulina cell-wall breaking shearing device
CN104450509B (en) * 2014-09-29 2016-02-03 江南大学 Spirulina cells broken wall shear
US11717602B2 (en) 2016-08-30 2023-08-08 Lifecell Corporation Systems and methods for medical device control
WO2019012231A1 (en) 2017-07-12 2019-01-17 Stemcis Tissue mechanical fragmentation device intended for the preparation of a composition of isolated cells, and corresponding method
FR3068986A1 (en) * 2017-07-12 2019-01-18 Stemcis DEVICE FOR MECHANICAL FRAGMENTATION OF TISSUES FOR THE PREPARATION OF A COMPOSITION OF ISOLATED CELLS, CORRESPONDING PROCESS
WO2019145029A1 (en) * 2018-01-24 2019-08-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Device and method for dissociating tissue

Also Published As

Publication number Publication date
ITTO930706A1 (en) 1995-03-28
CA2171117C (en) 2004-11-23
ES2121230T3 (en) 1998-11-16
US5731199A (en) 1998-03-24
JPH09502925A (en) 1997-03-25
ATE169244T1 (en) 1998-08-15
AU676386B2 (en) 1997-03-06
DE69412272D1 (en) 1998-09-10
JP3604141B2 (en) 2004-12-22
EP0720513A1 (en) 1996-07-10
DE69412272T2 (en) 1999-03-04
AU7809894A (en) 1995-04-18
EP0720513B1 (en) 1998-08-05
DK0720513T3 (en) 1999-05-03
ITTO930706A0 (en) 1993-09-28
IT1260682B (en) 1996-04-22
CA2171117A1 (en) 1995-04-06

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