WO1996018731A3 - Isolation of nucleic acid - Google Patents

Isolation of nucleic acid Download PDF

Info

Publication number
WO1996018731A3
WO1996018731A3 PCT/GB1995/002893 GB9502893W WO9618731A3 WO 1996018731 A3 WO1996018731 A3 WO 1996018731A3 GB 9502893 W GB9502893 W GB 9502893W WO 9618731 A3 WO9618731 A3 WO 9618731A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
sample
isolation
support
bound
Prior art date
Application number
PCT/GB1995/002893
Other languages
French (fr)
Other versions
WO1996018731A2 (en
Inventor
Arne Helge Deggerdal
Frank Larsen
Original Assignee
Dynal As
Dzieglewska Hanna Eva
Arne Helge Deggerdal
Frank Larsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10765877&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1996018731(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to DE69533317T priority Critical patent/DE69533317T2/en
Priority to CA002207608A priority patent/CA2207608C/en
Priority to EP95940351A priority patent/EP0796327B1/en
Priority to AU41829/96A priority patent/AU706211B2/en
Priority to AT95940351T priority patent/ATE272110T1/en
Priority to US08/849,686 priority patent/US20040215011A1/en
Priority to JP51846396A priority patent/JP3787354B2/en
Application filed by Dynal As, Dzieglewska Hanna Eva, Arne Helge Deggerdal, Frank Larsen filed Critical Dynal As
Publication of WO1996018731A2 publication Critical patent/WO1996018731A2/en
Publication of WO1996018731A3 publication Critical patent/WO1996018731A3/en
Priority to US11/234,001 priority patent/US7173124B2/en
Priority to US11/671,426 priority patent/US20070190559A1/en
Priority to US12/054,332 priority patent/US7989614B2/en
Priority to US12/130,959 priority patent/US20090149646A1/en
Priority to US12/130,926 priority patent/US20090068724A1/en
Priority to US13/160,428 priority patent/US8691969B2/en
Priority to US14/183,999 priority patent/US20140291576A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Abstract

The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.
PCT/GB1995/002893 1994-12-12 1995-12-12 Isolation of nucleic acid WO1996018731A2 (en)

Priority Applications (14)

Application Number Priority Date Filing Date Title
DE69533317T DE69533317T2 (en) 1994-12-12 1995-12-12 ISOLATION OF NUCLEIC ACIDS
CA002207608A CA2207608C (en) 1994-12-12 1995-12-12 Isolation of nucleic acid
EP95940351A EP0796327B1 (en) 1994-12-12 1995-12-12 Isolation of nucleic acid
AU41829/96A AU706211B2 (en) 1994-12-12 1995-12-12 Isolation of nucleic acid
AT95940351T ATE272110T1 (en) 1994-12-12 1995-12-12 ISOLATION OF NUCLEIC ACIDS
US08/849,686 US20040215011A1 (en) 1994-12-12 1995-12-12 Isolation of nucleic acid
JP51846396A JP3787354B2 (en) 1994-12-12 1995-12-12 Nucleic acid isolation method
US11/234,001 US7173124B2 (en) 1994-12-12 2005-09-23 Isolation of nucleic acid
US11/671,426 US20070190559A1 (en) 1994-12-12 2007-02-05 Isolation of nucleic acid
US12/054,332 US7989614B2 (en) 1994-12-12 2008-03-24 Isolation of nucleic acid
US12/130,926 US20090068724A1 (en) 1994-12-12 2008-05-30 Isolation of Nucleic Acid
US12/130,959 US20090149646A1 (en) 1994-12-12 2008-05-30 Isolation of Nucleic Acid
US13/160,428 US8691969B2 (en) 1994-12-12 2011-06-14 Isolation of nucleic acid
US14/183,999 US20140291576A1 (en) 1994-12-12 2014-02-19 Isolation of nucleic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9425138.6 1994-12-12
GBGB9425138.6A GB9425138D0 (en) 1994-12-12 1994-12-12 Isolation of nucleic acid

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US08849686 A-371-Of-International 1995-12-12
US08/849,686 A-371-Of-International US20040215011A1 (en) 1994-12-12 1995-12-12 Isolation of nucleic acid
US11/234,001 Continuation US7173124B2 (en) 1994-12-12 2005-09-23 Isolation of nucleic acid

Publications (2)

Publication Number Publication Date
WO1996018731A2 WO1996018731A2 (en) 1996-06-20
WO1996018731A3 true WO1996018731A3 (en) 1996-09-12

Family

ID=10765877

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1995/002893 WO1996018731A2 (en) 1994-12-12 1995-12-12 Isolation of nucleic acid

Country Status (9)

Country Link
US (8) US20040215011A1 (en)
EP (1) EP0796327B1 (en)
JP (1) JP3787354B2 (en)
AT (1) ATE272110T1 (en)
AU (1) AU706211B2 (en)
CA (1) CA2207608C (en)
DE (1) DE69533317T2 (en)
GB (1) GB9425138D0 (en)
WO (1) WO1996018731A2 (en)

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US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples

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