WO1996020287A3 - Detection of nucleic acids by target-catalyzed product formation - Google Patents

Detection of nucleic acids by target-catalyzed product formation Download PDF

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Publication number
WO1996020287A3
WO1996020287A3 PCT/US1995/016771 US9516771W WO9620287A3 WO 1996020287 A3 WO1996020287 A3 WO 1996020287A3 US 9516771 W US9516771 W US 9516771W WO 9620287 A3 WO9620287 A3 WO 9620287A3
Authority
WO
WIPO (PCT)
Prior art keywords
fragment
polynucleotide analyte
detection
oligonucleotide
polynucleotide
Prior art date
Application number
PCT/US1995/016771
Other languages
French (fr)
Other versions
WO1996020287A2 (en
Inventor
Edwin F Ullman
Linda M Western
Samuel J Rose
Original Assignee
Behringwerke Ag
Edwin F Ullman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23429108&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1996020287(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Behringwerke Ag, Edwin F Ullman filed Critical Behringwerke Ag
Priority to DE69528670T priority Critical patent/DE69528670T2/en
Priority to EP95944648A priority patent/EP0842294B1/en
Publication of WO1996020287A2 publication Critical patent/WO1996020287A2/en
Publication of WO1996020287A3 publication Critical patent/WO1996020287A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Abstract

A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5'-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5'-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3' of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the present invention are also disclosed.
PCT/US1995/016771 1994-12-23 1995-12-22 Detection of nucleic acids by target-catalyzed product formation WO1996020287A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE69528670T DE69528670T2 (en) 1994-12-23 1995-12-22 DETECTION OF NUCLEIC ACIDS BY NUCLEASE-CATALYZED PRODUCT PRODUCTION
EP95944648A EP0842294B1 (en) 1994-12-23 1995-12-22 Detection of nucleic acids by nuclease-catalyzed product formation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36316994A 1994-12-23 1994-12-23
US08/363,169 1994-12-23

Publications (2)

Publication Number Publication Date
WO1996020287A2 WO1996020287A2 (en) 1996-07-04
WO1996020287A3 true WO1996020287A3 (en) 1996-09-06

Family

ID=23429108

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/016771 WO1996020287A2 (en) 1994-12-23 1995-12-22 Detection of nucleic acids by target-catalyzed product formation

Country Status (5)

Country Link
US (7) US5792614A (en)
EP (2) EP0842294B1 (en)
DE (1) DE69528670T2 (en)
ES (1) ES2185719T3 (en)
WO (1) WO1996020287A2 (en)

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Also Published As

Publication number Publication date
US6121001A (en) 2000-09-19
DE69528670T2 (en) 2004-03-11
US6110677A (en) 2000-08-29
EP0842294A2 (en) 1998-05-20
US20050191676A1 (en) 2005-09-01
EP0842294B1 (en) 2002-10-23
US6368803B1 (en) 2002-04-09
EP1260593A2 (en) 2002-11-27
ES2185719T3 (en) 2003-05-01
WO1996020287A2 (en) 1996-07-04
US5792614A (en) 1998-08-11
US20080213767A1 (en) 2008-09-04
EP1260593A3 (en) 2003-12-03
US20020155548A1 (en) 2002-10-24
DE69528670D1 (en) 2002-11-28

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