WO1996035717A3 - Soluble, cleavable substrates of the hepatitis c virus protease - Google Patents

Soluble, cleavable substrates of the hepatitis c virus protease Download PDF

Info

Publication number
WO1996035717A3
WO1996035717A3 PCT/US1996/006389 US9606389W WO9635717A3 WO 1996035717 A3 WO1996035717 A3 WO 1996035717A3 US 9606389 W US9606389 W US 9606389W WO 9635717 A3 WO9635717 A3 WO 9635717A3
Authority
WO
WIPO (PCT)
Prior art keywords
soluble
hepatitis
virus protease
cleavable substrates
substrates
Prior art date
Application number
PCT/US1996/006389
Other languages
French (fr)
Other versions
WO1996035717A2 (en
Inventor
Rumin Zhang
Michael G Murray
Lata Ramanathan
Original Assignee
Schering Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Schering Corp filed Critical Schering Corp
Priority to EP96915540A priority Critical patent/EP0827508A2/en
Priority to AU57292/96A priority patent/AU5729296A/en
Priority to JP8532843A priority patent/JPH10506539A/en
Priority to MX9708679A priority patent/MX9708679A/en
Publication of WO1996035717A2 publication Critical patent/WO1996035717A2/en
Publication of WO1996035717A3 publication Critical patent/WO1996035717A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

Soluble HCV nonstructural substrates of the HCV polyprotein.
PCT/US1996/006389 1995-05-12 1996-05-09 Soluble, cleavable substrates of the hepatitis c virus protease WO1996035717A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP96915540A EP0827508A2 (en) 1995-05-12 1996-05-09 Soluble, cleavable substrates of the hepatitis c virus protease
AU57292/96A AU5729296A (en) 1995-05-12 1996-05-09 Soluble, cleavable substrates of the hepatitis c virus prote ase
JP8532843A JPH10506539A (en) 1995-05-12 1996-05-09 Soluble cleavable substrate for hepatitis C virus protease
MX9708679A MX9708679A (en) 1995-05-12 1996-05-09 Soluble, cleavable substrates of the hepatitis c virus protease.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/439,747 1995-05-12
US08/439,747 US5767233A (en) 1995-05-12 1995-05-12 Soluble cleavable substrates of the hepatitis C virus protease

Publications (2)

Publication Number Publication Date
WO1996035717A2 WO1996035717A2 (en) 1996-11-14
WO1996035717A3 true WO1996035717A3 (en) 1997-01-03

Family

ID=23745980

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/006389 WO1996035717A2 (en) 1995-05-12 1996-05-09 Soluble, cleavable substrates of the hepatitis c virus protease

Country Status (7)

Country Link
US (1) US5767233A (en)
EP (1) EP0827508A2 (en)
JP (1) JPH10506539A (en)
AU (1) AU5729296A (en)
CA (1) CA2220556A1 (en)
MX (1) MX9708679A (en)
WO (1) WO1996035717A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6303567B1 (en) * 1995-03-14 2001-10-16 Praecis Pharmaceuticals, Inc . Modulators of β-amyloid peptide aggregation comprising D-amino acids
CA2220551A1 (en) * 1995-05-12 1996-11-14 Schering Corporation Surface plasmon resonance based enzymatic assay
IT1277914B1 (en) * 1995-08-22 1997-11-12 Angeletti P Ist Richerche Bio PROCEDURE TO PRODUCE - IN PURE FORM AND IN HIGH QUANTITIES - POLYPEPTIDES WITH THE PROTEOLYTIC ACTIVITY OF THE NS3 PROTEASE OF HCV, AND
US5990276A (en) * 1996-05-10 1999-11-23 Schering Corporation Synthetic inhibitors of hepatitis C virus NS3 protease
JPH11513890A (en) * 1996-05-10 1999-11-30 シェーリング コーポレイション Synthetic inhibitor of hepatitis C virus NS3 protease
WO1998016657A1 (en) 1996-10-17 1998-04-23 Chiron Corporation Protease regulator screening assay
US6251583B1 (en) * 1998-04-27 2001-06-26 Schering Corporation Peptide substrates for HCV NS3 protease assays
WO2000052048A1 (en) 1999-03-04 2000-09-08 Praecis Pharmaceuticals Incorporated Modulators of beta-amyloid peptide aggregation comprising d-amino acids
EP1214454A1 (en) * 1999-08-30 2002-06-19 Merck & Co., Inc. Hepatitis c virus replication inhibitors
US6818396B1 (en) * 2000-11-28 2004-11-16 Proteus S.A. Process for determination of the activity of a substance using an in vitro functional test
CA2457059A1 (en) * 2001-08-10 2003-02-20 Ahram Biosystems Inc. System for detecting protease
US8450552B2 (en) * 2009-05-18 2013-05-28 Exxonmobil Chemical Patents Inc. Pyrolysis reactor materials and methods
JP5818059B2 (en) * 2010-03-04 2015-11-18 国立大学法人 長崎大学 How to identify viruses
EP3914702A4 (en) * 2019-01-25 2022-10-26 Senti Biosciences, Inc. Fusion constructs for controlling protein function

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015575A1 (en) * 1990-04-04 1991-10-17 Chiron Corporation Hepatitis c virus protease
WO1993025575A1 (en) * 1992-06-10 1993-12-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Peptide for stimulation of cytotoxic t lymphocytes specific for hepatitis c virus
DE4240980A1 (en) * 1992-08-07 1994-02-10 Boehringer Mannheim Gmbh HCV peptide antigens and method for the determination of HCV
WO1995022985A1 (en) * 1994-02-23 1995-08-31 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Method for reproducing in vitro the proteolytic activity of the ns3 protease of hepatitis c virus (hcv)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5312737A (en) * 1988-03-11 1994-05-17 Abbott Laboratories CKS method of HCV protein synthesis
US5372928A (en) * 1989-09-15 1994-12-13 Chiron Corporation Hepatitis C virus isolates

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015575A1 (en) * 1990-04-04 1991-10-17 Chiron Corporation Hepatitis c virus protease
WO1993025575A1 (en) * 1992-06-10 1993-12-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Peptide for stimulation of cytotoxic t lymphocytes specific for hepatitis c virus
DE4240980A1 (en) * 1992-08-07 1994-02-10 Boehringer Mannheim Gmbh HCV peptide antigens and method for the determination of HCV
WO1995022985A1 (en) * 1994-02-23 1995-08-31 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Method for reproducing in vitro the proteolytic activity of the ns3 protease of hepatitis c virus (hcv)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
C. LIN ET AL.: "Hepatitis C virus NS3 serine proteinase", J. VIROL., vol. 68, no. 12, 1994, pages 8147 - 8157, XP002018452 *
C. STEINKUHLER ET AL.: "In vitro activity of hepatitis C virus protease NS3 purified from recombinant baculovirus-infected Sf9 cells", J. BIOL. CHEM., vol. 271, no. 11, 1996, pages 6367 - 6373, XP002018451 *
K. SUDO ET AL.: "Establishment of an in vitro assay system for screening hepatitis C protease inhibitors using high performance liquid chromatography", ANTIVIRAL RESEARCH, vol. 32, 1996, pages 9 - 18, XP000608038 *

Also Published As

Publication number Publication date
US5767233A (en) 1998-06-16
WO1996035717A2 (en) 1996-11-14
AU5729296A (en) 1996-11-29
CA2220556A1 (en) 1996-11-14
MX9708679A (en) 1998-02-28
EP0827508A2 (en) 1998-03-11
JPH10506539A (en) 1998-06-30

Similar Documents

Publication Publication Date Title
AU5729196A (en) Soluble, active hepatitis c virus protease
ZA979327B (en) Inhibitors of serine proteases, particularly hepatitis C virus NS3 protease.
WO1996035717A3 (en) Soluble, cleavable substrates of the hepatitis c virus protease
AU2055301A (en) Alpha-ketoamide inhibitors of hepatitis c virus ns3 protease
WO1993000365A3 (en) Hepatitis c virus (hcv) polypeptides
WO2004014852A3 (en) Iminothiazolidinones as inhibitors of hcv replication
AU7063598A (en) Hepatitis c ns3 protease inhibitors
AU6371900A (en) Lactam inhibitors of hepatitis c virus ns3 protease
AU2933797A (en) Synthetic inhibitors of hepatitis c virus ns3 protease
AU2002230763A1 (en) Inhibitors of hepatitis c virus ns3 protease
AU2001245356A1 (en) Inhibitors of hepatitis c virus ns3 protease
WO1994023041A3 (en) Method for selective inactivation of viral replication
AU652941B2 (en) Non-A, Non-B hepatitis virus particles
AU3714499A (en) Hepatitis c virus mimotopes
YU5800A (en) A universally applicable blood plasma
SI0932617T1 (en) Inhibitors of serine proteases, particularly hepatitis c virus ns3 protease
CA2220729A1 (en) Method for refolding insoluble aggregates of hepatitis c virus protease
WO1997040147A8 (en) Antigenically reactive regions of the hepatitis a virus polyprotein
IL143798A0 (en) Hepatitis c virus ns3 protease
AU4397097A (en) Soluble polypeptides with activity of the ns3 serine protease of hepatitis c virus, and process for their preparation and isolation
AU5924396A (en) Compositions and methods for the diagnosis of, and vaccinati on against, hepatitis c virus (hcv)
AU5069800A (en) Novel gbv sequence
WO1995025806A3 (en) Packaging-deficient lentiviruses
EP0593291A3 (en) Nonstructural protein of hepatitis C virus, and diagnostic method and kit using the same.
AU5397498A (en) Sequences derived from the genome of the c hepatitis virus, and use thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AU AZ BB BG BR BY CA CN CZ EE GE HU IS JP KG KR KZ LK LR LT LV MD MG MK MN MX NO NZ PL RO RU SG SI SK TJ TM TR TT UA UZ VN AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AU AZ BB BG BR BY CA CN CZ EE GE HU IS JP KG KR KZ LK LR LT LV MD MG MK MN MX NO NZ PL RO RU SG SI SK TJ TM TR TT UA UZ VN AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
ENP Entry into the national phase

Ref document number: 2220556

Country of ref document: CA

Ref country code: CA

Ref document number: 2220556

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: PA/a/1997/008679

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 1996915540

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1996915540

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1996915540

Country of ref document: EP