WO1998006836A3 - Expression system derived from the lipase regulation cascade of pseudomonas alcaligenes - Google Patents

Expression system derived from the lipase regulation cascade of pseudomonas alcaligenes Download PDF

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Publication number
WO1998006836A3
WO1998006836A3 PCT/US1997/014450 US9714450W WO9806836A3 WO 1998006836 A3 WO1998006836 A3 WO 1998006836A3 US 9714450 W US9714450 W US 9714450W WO 9806836 A3 WO9806836 A3 WO 9806836A3
Authority
WO
WIPO (PCT)
Prior art keywords
pseudomonas alcaligenes
expression system
alcaligenes
expression
regulation cascade
Prior art date
Application number
PCT/US1997/014450
Other languages
French (fr)
Other versions
WO1998006836A9 (en
WO1998006836A2 (en
Inventor
Gijsbert Gerritse
Wilhelmus J Quax
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Priority to DE69731210T priority Critical patent/DE69731210T2/en
Priority to AT97938360T priority patent/ATE279513T1/en
Priority to CA2262510A priority patent/CA2262510C/en
Priority to DK04015305T priority patent/DK1557467T3/en
Priority to DK97938360T priority patent/DK0918851T3/en
Priority to DK04015824T priority patent/DK1555320T3/en
Priority to EP97938360A priority patent/EP0918851B1/en
Priority to AU40709/97A priority patent/AU4070997A/en
Publication of WO1998006836A2 publication Critical patent/WO1998006836A2/en
Publication of WO1998006836A3 publication Critical patent/WO1998006836A3/en
Publication of WO1998006836A9 publication Critical patent/WO1998006836A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Abstract

An expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.
PCT/US1997/014450 1996-08-16 1997-08-15 Expression system derived from the lipase regulation cascade of pseudomonas alcaligenes WO1998006836A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
DE69731210T DE69731210T2 (en) 1996-08-16 1997-08-15 EXPRESSION SYSTEM DERIVED FROM THE LIPASE REGULATORY CASCADE OF PSEUDONOMAS ALCALIGENES
AT97938360T ATE279513T1 (en) 1996-08-16 1997-08-15 EXPRESSION SYSTEM DERIVED FROM THE LIPASE REGULATORY CAScade OF PSEUDONOMAS ALCALIGENES
CA2262510A CA2262510C (en) 1996-08-16 1997-08-15 Expression system derived from the lipase regulation cascade of pseudomonas alcaligenes
DK04015305T DK1557467T3 (en) 1996-08-16 1997-08-15 Expression system derived from the lipase regulation cascade of Psedomonas alcalignenes
DK97938360T DK0918851T3 (en) 1996-08-16 1997-08-15 Expression system for altered expression levels
DK04015824T DK1555320T3 (en) 1996-08-16 1997-08-15 Expression system arising from the lipase regulation cascade in Pseudomonas alcaligenes
EP97938360A EP0918851B1 (en) 1996-08-16 1997-08-15 Expression system for altered expression levels
AU40709/97A AU4070997A (en) 1996-08-16 1997-08-15 Expression system for altered expression levels

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69909296A 1996-08-16 1996-08-16
US08/699,092 1996-08-16

Publications (3)

Publication Number Publication Date
WO1998006836A2 WO1998006836A2 (en) 1998-02-19
WO1998006836A3 true WO1998006836A3 (en) 1998-04-30
WO1998006836A9 WO1998006836A9 (en) 1998-06-25

Family

ID=24807894

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/014450 WO1998006836A2 (en) 1996-08-16 1997-08-15 Expression system derived from the lipase regulation cascade of pseudomonas alcaligenes

Country Status (8)

Country Link
US (3) US6048710A (en)
EP (3) EP1557467B1 (en)
AT (3) ATE396268T1 (en)
AU (1) AU4070997A (en)
CA (1) CA2262510C (en)
DE (3) DE69731210T2 (en)
DK (3) DK1555320T3 (en)
WO (1) WO1998006836A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19848016A1 (en) * 1998-10-17 2000-04-20 Roche Diagnostics Gmbh Production of extracellular microbial protein, particularly esterolytic enzymes, in heterologous bacteria transformed with appropriate nucleic acid and secretory apparatus
PL2336153T3 (en) * 2003-11-21 2016-08-31 Pfenex Inc Improved expression systems with SEC-system secretion
CN111019904A (en) * 2018-10-10 2020-04-17 安徽东方帝维生物制品股份有限公司 Construction method of immortalized sheep testis cell line adapting to sheep aphtha virus proliferation

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994002617A2 (en) * 1992-07-23 1994-02-03 Gist-Brocades N.V. Cloning and expression of a lipase modulator gene from pseudomonas pseudoalcaligenes
WO1995030744A2 (en) * 1994-05-04 1995-11-16 Genencor International Inc. Lipases with improved surfactant resistance
US5529917A (en) * 1985-08-09 1996-06-25 Gist-Brocades Compositions and methods for making lipolytic enzymes
WO1997013847A1 (en) * 1995-10-12 1997-04-17 Genencor International, Inc. Expression system, vector and cell transformed thereby

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3079276B2 (en) * 1988-02-28 2000-08-21 天野製薬株式会社 Recombinant DNA, Pseudomonas sp. Containing the same, and method for producing lipase using the same
US5756315A (en) * 1989-12-05 1998-05-26 Kyowa Hakko Kogyo Co., Ltd. Inosine-guanosine kinase
IE911869A1 (en) * 1990-06-01 1991-12-04 Regeneron Pharma A family of map2 protein kinases
US6514745B1 (en) * 1993-07-19 2003-02-04 The Regents Of The University Of California Oncoprotein protein kinase

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5529917A (en) * 1985-08-09 1996-06-25 Gist-Brocades Compositions and methods for making lipolytic enzymes
WO1994002617A2 (en) * 1992-07-23 1994-02-03 Gist-Brocades N.V. Cloning and expression of a lipase modulator gene from pseudomonas pseudoalcaligenes
WO1995030744A2 (en) * 1994-05-04 1995-11-16 Genencor International Inc. Lipases with improved surfactant resistance
WO1997013847A1 (en) * 1995-10-12 1997-04-17 Genencor International, Inc. Expression system, vector and cell transformed thereby

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
AKRIM M. ET AL.: "Xcp-mediated protein secretion in Pseudomonas aeruginosa: identification of two additional genes and evidence for regulation of xcp gene expression.", MOLECULAR MICROBIOLOGY, vol. 10, no. 2, 1993, pages 431 - 443, XP002054741 *
BALLY M. ET AL.: "Protein secretion in Pseudomonas aeruginosa: characterization of seven xcp genes and processing of secretory apparatus components by prepilin peptidase.", MOLECULAR MICROBIOLOGY, vol. 6, no. 9, 1992, pages 1121 - 1131, XP002054738 *
FOGLINO M. ET AL.: "A direct sufhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa.", MICROBIOLOGY, vol. 141, 1995, pages 431 - 439, XP002054742 *
JAEGER K.-E. ET AL.: "Lipase of Pseudomonas aeruginosa: Molecular Biology and Biotechnological Application.", MOLECULAR BIOLOGY OF PSEUDOMONADS, vol. 5, 1996, pages 319 - 330, XP002054739 *
LATIFI A. ET AL.: "Multiple homologues of LuxR and LuxI control expression of virulence determinants and secondary metabolites through quorum sensing in Pseudomonas aeruginosa PAO1.", MOLECULAR MICROBIOLOGY, vol. 17, no. 2, 1995, pages 333 - 343, XP002054743 *
NUNN D.N. AND LORY S.: "Components of the protein-excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidase.", PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 47 - 51, XP002054740 *
PEREZ-MARTIN J. AND DE LORENZO V.: "Integration host factor suppresses promiscuous activation of the sigma(54)-dependent promoter Pu of Pseudomonas putida.", PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 7277 - 7281, XP002054737 *
TOTTEN P.A. ET AL.: "The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene.", JOURNAL OF BACTERIOLOGY, vol. 172, no. 1, 1990, pages 389 - 396, XP002054976 *

Also Published As

Publication number Publication date
US6225106B1 (en) 2001-05-01
EP0918851B1 (en) 2004-10-13
EP1555320B1 (en) 2008-05-21
CA2262510C (en) 2012-10-23
EP0918851A2 (en) 1999-06-02
US6313283B1 (en) 2001-11-06
EP1557467B1 (en) 2008-05-21
DE69731210D1 (en) 2004-11-18
DE69731210T2 (en) 2005-10-20
DE69738724D1 (en) 2008-07-03
CA2262510A1 (en) 1998-02-19
ATE396268T1 (en) 2008-06-15
WO1998006836A2 (en) 1998-02-19
DK0918851T3 (en) 2005-01-24
DE69738723D1 (en) 2008-07-03
EP1557467A1 (en) 2005-07-27
EP1555320A3 (en) 2005-07-27
ATE279513T1 (en) 2004-10-15
DK1555320T3 (en) 2008-09-29
US6048710A (en) 2000-04-11
AU4070997A (en) 1998-03-06
DK1557467T3 (en) 2008-09-15
ATE396267T1 (en) 2008-06-15
EP1555320A2 (en) 2005-07-20

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