WO1999023250A3 - Spezifisches und sensitives nukleinsäurenachweisverfahren - Google Patents

Spezifisches und sensitives nukleinsäurenachweisverfahren Download PDF

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Publication number
WO1999023250A3
WO1999023250A3 PCT/EP1998/006961 EP9806961W WO9923250A3 WO 1999023250 A3 WO1999023250 A3 WO 1999023250A3 EP 9806961 W EP9806961 W EP 9806961W WO 9923250 A3 WO9923250 A3 WO 9923250A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequence
nucleic acid
bind
specific
nucleic acids
Prior art date
Application number
PCT/EP1998/006961
Other languages
English (en)
French (fr)
Other versions
WO1999023250A2 (de
Inventor
Christoph Kessler
Gerd Haberhausen
Knut Bartl
Henrik Orum
Original Assignee
Roche Diagnostics Gmbh
Christoph Kessler
Gerd Haberhausen
Knut Bartl
Henrik Orum
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19748690A external-priority patent/DE19748690A1/de
Priority claimed from DE19814001A external-priority patent/DE19814001A1/de
Priority claimed from DE19814828A external-priority patent/DE19814828A1/de
Application filed by Roche Diagnostics Gmbh, Christoph Kessler, Gerd Haberhausen, Knut Bartl, Henrik Orum filed Critical Roche Diagnostics Gmbh
Priority to JP2000519105A priority Critical patent/JP2002505071A/ja
Priority to CA002308762A priority patent/CA2308762A1/en
Priority to EP98965653A priority patent/EP1029084A2/de
Priority to AU21521/99A priority patent/AU741141B2/en
Publication of WO1999023250A2 publication Critical patent/WO1999023250A2/de
Publication of WO1999023250A3 publication Critical patent/WO1999023250A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

Gegenstand der Erfindung ist ein Verfahren zum Nachweis einer Nukleinsäure enthaltend die Schritte Herstellung einer Vielzahl von Amplifikaten eines Teilstücks dieser Nukleinsäure mit einer Länge von weniger als 100 Nukleotiden mit Hilfe zweier Primer, von denen einer an eine erste Bindesequenz (A) eines Strangs der Nukleinsäure binden kann und von denen der andere an eine zweite Bindesequenz (C'), die zu einer mit (A) nicht überlappenden, in 3'-Richtung von (A) gelegenen Sequenz (C) im wesentlichen komplementär ist, binden kann, in Anwesenheit einer Sonde mit einer Bindesequenz (D), welche an die zwischen den Sequenzen (A) und (C) gelegene dritte Sequenz (B) oder das Komplement (B') davon binden kann, wobei diese Sonde eine Reportergruppe und eine Quenchergruppe enthält, unter Verwendung einer Polymerase mit 5'-Nukleaseaktivität und Nachweis der Nukleinsäure durch Messung eines Signals, welches durch die Freisetzung der Reportergruppe bedingt ist.
PCT/EP1998/006961 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren WO1999023250A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000519105A JP2002505071A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法
CA002308762A CA2308762A1 (en) 1997-11-04 1998-11-03 Specific and sensitive nucleic acid detection method
EP98965653A EP1029084A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren
AU21521/99A AU741141B2 (en) 1997-11-04 1998-11-03 Specific and sensitive method for detecting nucleic acids

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE19748690A DE19748690A1 (de) 1997-11-04 1997-11-04 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814001A DE19814001A1 (de) 1998-03-28 1998-03-28 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814001.0 1998-04-02
DE19814828.3 1998-04-02
DE19748690.8 1998-04-02
DE19814828A DE19814828A1 (de) 1998-04-02 1998-04-02 Spezifisches und sensitives Nukleinsäurenachweisverfahren

Publications (2)

Publication Number Publication Date
WO1999023250A2 WO1999023250A2 (de) 1999-05-14
WO1999023250A3 true WO1999023250A3 (de) 1999-07-22

Family

ID=27217887

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/EP1998/006961 WO1999023250A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren
PCT/EP1998/006951 WO1999024606A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren
PCT/EP1998/006952 WO1999023249A2 (de) 1997-11-04 1998-11-03 Spezifisches und empfindliches nukleinsäurenachweisverfahren

Family Applications After (2)

Application Number Title Priority Date Filing Date
PCT/EP1998/006951 WO1999024606A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren
PCT/EP1998/006952 WO1999023249A2 (de) 1997-11-04 1998-11-03 Spezifisches und empfindliches nukleinsäurenachweisverfahren

Country Status (6)

Country Link
US (1) US7105318B2 (de)
EP (3) EP1029084A2 (de)
JP (3) JP2002509694A (de)
AU (3) AU1232099A (de)
CA (3) CA2308368C (de)
WO (3) WO1999023250A2 (de)

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EP1038022B1 (de) * 1997-12-12 2005-07-20 Digene Corporation Bewertung von humaner papillomvirus verwandter krankheit
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7807802B2 (en) 2002-11-12 2010-10-05 Abbott Lab Polynucleotides for the amplification and detection of Chlamydia trachomatis and Neisseria gonorrhoeae
EP2121956B1 (de) 2006-12-21 2016-08-17 Gen-Probe Incorporated Verfahren und zusammensetzungen zur amplizierung von nukleinsäuren
CN101680027A (zh) * 2007-03-16 2010-03-24 454生命科学公司 检测hiv耐药变异体的系统和方法
GB0714389D0 (en) 2007-07-21 2007-09-05 Barry Callebaut Ag Process and product
JP2011518333A (ja) * 2008-04-17 2011-06-23 キアジェン ゲイサーズバーグ インコーポレイテッド 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット
WO2010062556A1 (en) 2008-10-27 2010-06-03 Qiagen Gaithersburg Inc. Fast results hybrid capture assay and system
ES2644516T3 (es) * 2009-01-28 2017-11-29 Qiagen Gaithersburg, Inc. Método y ensayo de preparación de muestras de gran volumen específico de secuencia
CN102414327B (zh) * 2009-05-01 2015-04-08 奇亚根盖瑟斯堡股份有限公司 用于在样品中检测rna剪接形式的非靶向扩增方法
WO2011003020A1 (en) 2009-07-01 2011-01-06 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
WO2011032124A1 (en) 2009-09-14 2011-03-17 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
WO2011094528A2 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an hpv in a sample
JP6103941B2 (ja) 2010-01-29 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド 核酸の配列特異的精製および多重分析のための方法および組成物
WO2011146629A2 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
US9885092B2 (en) 2011-02-24 2018-02-06 Qiagen Gaithersburg Inc. Materials and methods for detection of HPV nucleic acids
WO2022230214A1 (ja) * 2021-04-28 2022-11-03 バイオニクス株式会社 光学測定装置

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EP0229701A2 (de) * 1986-01-10 1987-07-22 F. Hoffmann-La Roche Ag Nachweis von Viren durch Verstärkung und Hybridisation
WO1991010675A1 (en) * 1990-01-19 1991-07-25 Stichting Researchfonds Pathologie Primers and process for detecting human papillomavirus genotypes by pcr
WO1995002690A1 (en) * 1993-07-13 1995-01-26 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
US5527898A (en) * 1988-09-09 1996-06-18 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe

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EP0229701A2 (de) * 1986-01-10 1987-07-22 F. Hoffmann-La Roche Ag Nachweis von Viren durch Verstärkung und Hybridisation
US5527898A (en) * 1988-09-09 1996-06-18 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
WO1991010675A1 (en) * 1990-01-19 1991-07-25 Stichting Researchfonds Pathologie Primers and process for detecting human papillomavirus genotypes by pcr
WO1995002690A1 (en) * 1993-07-13 1995-01-26 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe

Also Published As

Publication number Publication date
EP1029083A2 (de) 2000-08-23
WO1999023250A2 (de) 1999-05-14
WO1999024606A2 (de) 1999-05-20
JP2002505071A (ja) 2002-02-19
CA2308762A1 (en) 1999-05-14
WO1999023249A2 (de) 1999-05-14
AU741141B2 (en) 2001-11-22
US20030175765A1 (en) 2003-09-18
CA2312779A1 (en) 1999-05-20
US7105318B2 (en) 2006-09-12
EP1029077A2 (de) 2000-08-23
AU2152099A (en) 1999-05-31
CA2308368C (en) 2009-01-20
WO1999024606A3 (de) 1999-07-22
JP2002509694A (ja) 2002-04-02
WO1999023249A3 (de) 1999-09-10
AU2152199A (en) 1999-05-24
AU1232099A (en) 1999-05-24
CA2308368A1 (en) 1999-05-14
EP1029084A2 (de) 2000-08-23
JP2001521765A (ja) 2001-11-13

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