WO1999060110A3 - Stable envelope proteins for retroviral, viral and liposome vectors and use in gene and drug therapy - Google Patents

Stable envelope proteins for retroviral, viral and liposome vectors and use in gene and drug therapy Download PDF

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Publication number
WO1999060110A3
WO1999060110A3 PCT/US1999/011155 US9911155W WO9960110A3 WO 1999060110 A3 WO1999060110 A3 WO 1999060110A3 US 9911155 W US9911155 W US 9911155W WO 9960110 A3 WO9960110 A3 WO 9960110A3
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WO
WIPO (PCT)
Prior art keywords
envelope
mutants
retroviral
gene
viral
Prior art date
Application number
PCT/US1999/011155
Other languages
French (fr)
Other versions
WO1999060110A2 (en
WO1999060110A9 (en
Inventor
Lorraine M Albritton
Tatiana Zavorotinskaya
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Univ Tennessee Res Corp
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Publication date
Application filed by Univ Tennessee Res Corp filed Critical Univ Tennessee Res Corp
Priority to AU40898/99A priority Critical patent/AU4089899A/en
Publication of WO1999060110A2 publication Critical patent/WO1999060110A2/en
Publication of WO1999060110A9 publication Critical patent/WO1999060110A9/en
Publication of WO1999060110A3 publication Critical patent/WO1999060110A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13045—Special targeting system for viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13061—Methods of inactivation or attenuation
    • C12N2740/13062—Methods of inactivation or attenuation by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6045—RNA rev transcr viruses
    • C12N2810/6054—Retroviridae

Abstract

This invention includes retrovirus envelope mutants into which heterologous peptide or glycopeptide sequences can be linked for expression and stable presentation on retroviral vectors. The envelope mutants are characterized by the ability to restore the target penetration capability that is lost or greatly diminished upon fusion of heterologous sequences to the wild type envelope protein and the ability to increase the fusion envelope protein stability and decrease envelope shedding from virus particles. The envelope mutants are created by rotating residues in at least one of 7 motifs. The disclosed envelope proteins also can be used in liposome or pseudotype-virus compositions for delivery of agents including nucleic acid molecules. Methods of preparing and utilizing these envelope mutants in gene therapy are also described.
PCT/US1999/011155 1998-05-20 1999-05-20 Stable envelope proteins for retroviral, viral and liposome vectors and use in gene and drug therapy WO1999060110A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU40898/99A AU4089899A (en) 1998-05-20 1999-05-20 Stable envelope proteins for retroviral, viral and liposome vectors and use in gene and drug therapy

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8614998P 1998-05-20 1998-05-20
US60/086,149 1998-05-20

Publications (3)

Publication Number Publication Date
WO1999060110A2 WO1999060110A2 (en) 1999-11-25
WO1999060110A9 WO1999060110A9 (en) 2000-02-17
WO1999060110A3 true WO1999060110A3 (en) 2000-04-13

Family

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Family Applications (1)

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PCT/US1999/011155 WO1999060110A2 (en) 1998-05-20 1999-05-20 Stable envelope proteins for retroviral, viral and liposome vectors and use in gene and drug therapy

Country Status (3)

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US (1) US6448390B1 (en)
AU (1) AU4089899A (en)
WO (1) WO1999060110A2 (en)

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EP1932426A1 (en) * 1998-10-01 2008-06-18 University of Southern California Gene delivery system and methods of use
EP1811036B8 (en) * 1999-02-22 2011-09-21 Georgetown University Antibody fragment-targeted immunoliposomes for systemic gene delivery
AU2001258659A1 (en) * 2000-05-30 2001-12-11 Ich Productions Limited Improved methods of transfection
WO2002022663A2 (en) * 2000-09-18 2002-03-21 Maxygen, Inc. Stress resistant retroviruses
US20050100890A1 (en) * 2003-10-15 2005-05-12 Davidson Beverly L. Methods for producing and using in vivo pseudotyped retroviruses
WO2007095201A2 (en) * 2006-02-15 2007-08-23 The Regents Of The University Of California Pseudotyped retroviral vectors and methods of use thereof
US20100076061A1 (en) * 2008-04-30 2010-03-25 University Of Iowa Research Foundation Repeated administration of lentiviral vectors to respiratory cells
US8829173B2 (en) 2008-09-26 2014-09-09 Tocagen Inc. Recombinant vectors
WO2010045002A2 (en) 2008-09-26 2010-04-22 Tocagen Inc. Gene therapy vectors and cytosine deaminases
US9669049B2 (en) 2010-10-31 2017-06-06 Tocagen Inc. Compositions comprising gamma retrovirus vectors and methods of treating proliferative disorders
EP2691530B1 (en) 2011-06-10 2018-03-07 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
WO2014066700A1 (en) 2012-10-25 2014-05-01 Tocagen Inc. Retroviral vector with mini-promoter cassette
US9642921B2 (en) 2012-12-20 2017-05-09 Tocagen Inc. Cancer combination therapy and recombinant vectors
EA201890640A1 (en) 2015-09-04 2018-09-28 Токаджен Инк. RECOMBINANT VECTORS CONTAINING PEPTIDE 2A

Citations (4)

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WO1998011243A2 (en) * 1996-09-11 1998-03-19 The General Hospital Corporation Expression of an exogenous gene in a mammalian cell by use of a non-mammalian dna virus having an altered coat protein
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WO1999060110A2 (en) 1999-11-25
US6448390B1 (en) 2002-09-10
AU4089899A (en) 1999-12-06
WO1999060110A9 (en) 2000-02-17

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