WO1999060390A3 - Electrophoresis sequencing apparatus - Google Patents

Electrophoresis sequencing apparatus Download PDF

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Publication number
WO1999060390A3
WO1999060390A3 PCT/US1999/015901 US9915901W WO9960390A3 WO 1999060390 A3 WO1999060390 A3 WO 1999060390A3 US 9915901 W US9915901 W US 9915901W WO 9960390 A3 WO9960390 A3 WO 9960390A3
Authority
WO
WIPO (PCT)
Prior art keywords
allows
sample
lanes
lane
gel
Prior art date
Application number
PCT/US1999/015901
Other languages
French (fr)
Other versions
WO1999060390A9 (en
WO1999060390A2 (en
Inventor
Robert W Flesher
Kevin J Barnes
Original Assignee
Apogee Designs Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Apogee Designs Ltd filed Critical Apogee Designs Ltd
Publication of WO1999060390A2 publication Critical patent/WO1999060390A2/en
Publication of WO1999060390A9 publication Critical patent/WO1999060390A9/en
Publication of WO1999060390A3 publication Critical patent/WO1999060390A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44708—Cooling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples

Abstract

A compact, low cost, automated electrophoresis apparatus provides for the optical detection of multiple DNA samples. A substantially cylindrical gel cartridge houses multiple sample lanes that are sealed on the outer periphery by a shrink tube, which allows for straightforward gel casting, as well as for the pre-casting of sequencing gels. The radial column design of the gel cartridge also facilitates the use of a light source to illuminate one or more sample lanes at the same time. The radial column design further provides for an increased sample lane depth that allows for reading through a greater optical path length of the migrated samples, yielding greater optical signal strength, and an increased signal to noise ratio. The gel lanes can be designed to vary in depth as a function of longitudinal length, allowing for a variable current density. Additionally, this radial design allows for different types of optical detection techniques to be performed on the sample, including transmittance and fluorescence. A system of cooling channels incorporated into the radial column design allows for a coolant to be recirculated in the cooling channels between the actual running sample lanes for better lane-to-lane thermal control. This cooling system allows higher operating voltages and facilitates the use of multiple thermal zones, as required, for instance, for single-strand conformation polymorphism (SSCP) applications.
PCT/US1999/015901 1998-04-24 1999-04-26 Electrophoresis sequencing apparatus WO1999060390A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/065,901 1998-04-24
US09/065,901 US6113767A (en) 1998-04-24 1998-04-24 Electrophoresis sequencing apparatus

Publications (3)

Publication Number Publication Date
WO1999060390A2 WO1999060390A2 (en) 1999-11-25
WO1999060390A9 WO1999060390A9 (en) 2000-02-24
WO1999060390A3 true WO1999060390A3 (en) 2001-05-31

Family

ID=22065907

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/015901 WO1999060390A2 (en) 1998-04-24 1999-04-26 Electrophoresis sequencing apparatus

Country Status (2)

Country Link
US (1) US6113767A (en)
WO (1) WO1999060390A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002237206A1 (en) * 2001-03-07 2002-09-19 Nkt Research A/S Method for the manufacture of gradient gels and method for analysing biomolecules on ultra-long ipg gels
US6974528B2 (en) * 2001-07-24 2005-12-13 Biomics, Inc. High performance wide bore electrophoresis

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3867271A (en) * 1973-08-13 1975-02-18 Hoefer Scientific Instr Gel electrophoresis apparatus and method
DE3034899A1 (en) * 1980-09-16 1982-04-29 Walter Dr. 7800 Freiburg Littke Continuous electrophoresis appts. has tubes for gel - fitted in with split sealing rings using handling device
US4374723A (en) * 1978-11-13 1983-02-22 C. Desaga Gmbh Nachf. Erich Fecht Apparatus for electrophoresis
US4375401A (en) * 1981-05-20 1983-03-01 Nicholas Catsimpoolas Electrophoresis system
EP0280567A2 (en) * 1987-02-26 1988-08-31 Fuji Photo Film Co., Ltd. Means for electrophoresis
EP0475555A1 (en) * 1990-09-11 1992-03-18 Bioseparations, Inc. Multi-modality electrical separator apparatus and method
US5483075A (en) * 1994-11-01 1996-01-09 Perkin-Elmer Corporation Rotary scanning apparatus

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3129158A (en) * 1961-01-24 1964-04-14 Raymond Samuel Process for gel electrophoresis
US3290240A (en) * 1962-03-14 1966-12-06 Edward J Neren Vertical electrophoresis apparatus with adjustable media tube
US3208929A (en) * 1962-09-11 1965-09-28 Robert H Raymond Apparatus for gel electrophoresis
US3956099A (en) * 1972-10-30 1976-05-11 Louis Israel Continuous preparative electrophoresis apparatus
US3927826A (en) * 1974-08-27 1975-12-23 Us Health Rotor for centrifugal testing of electrophoresis gel
US3980546A (en) * 1975-06-27 1976-09-14 Caccavo Francis A Micropreparative electrophoresis apparatus
US4048049A (en) * 1976-11-19 1977-09-13 Hoefer Scientific Instruments Tube gel electrophoresis device
US5190629A (en) * 1987-07-16 1993-03-02 Fuji Photo Film Co., Ltd. Electrophoresis medium membrane
US4834854A (en) * 1987-08-20 1989-05-30 Fuji Photo Film Co., Ltd. Method of making an electrophoresis medium
US4908112A (en) * 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
JP2853745B2 (en) * 1989-04-12 1999-02-03 株式会社日立製作所 Light detection electrophoresis device
US5529679A (en) * 1992-02-28 1996-06-25 Hitachi, Ltd. DNA detector and DNA detection method
US5730850A (en) * 1993-04-23 1998-03-24 Hitachi, Ltd. Capillary array electrophoresis system
US5439578A (en) * 1993-06-03 1995-08-08 The Governors Of The University Of Alberta Multiple capillary biochemical analyzer
US5332480A (en) * 1993-06-16 1994-07-26 University Of Iowa Research Foundation Capillary bed electrophoresis
JP3034770B2 (en) * 1994-09-29 2000-04-17 日立ソフトウエアエンジニアリング株式会社 Capillary electrophoresis device
US5900132A (en) * 1995-03-23 1999-05-04 Beckman Coulter, Inc. Capillary holder
US5885430A (en) * 1996-10-04 1999-03-23 Spectrumedix Corporation Capillary tube holder for an electrophoretic apparatus
US5833826A (en) * 1996-12-13 1998-11-10 The Perkin-Elmer Corporation Method and apparatus for reducing the distortion of a sample zone eluting from a capillary electrophoresis capillary
US5903348A (en) * 1997-03-12 1999-05-11 Nz Applied Technologies, Inc. System and method for molecular sample measurements

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3867271A (en) * 1973-08-13 1975-02-18 Hoefer Scientific Instr Gel electrophoresis apparatus and method
US4374723A (en) * 1978-11-13 1983-02-22 C. Desaga Gmbh Nachf. Erich Fecht Apparatus for electrophoresis
DE3034899A1 (en) * 1980-09-16 1982-04-29 Walter Dr. 7800 Freiburg Littke Continuous electrophoresis appts. has tubes for gel - fitted in with split sealing rings using handling device
US4375401A (en) * 1981-05-20 1983-03-01 Nicholas Catsimpoolas Electrophoresis system
EP0280567A2 (en) * 1987-02-26 1988-08-31 Fuji Photo Film Co., Ltd. Means for electrophoresis
EP0475555A1 (en) * 1990-09-11 1992-03-18 Bioseparations, Inc. Multi-modality electrical separator apparatus and method
US5483075A (en) * 1994-11-01 1996-01-09 Perkin-Elmer Corporation Rotary scanning apparatus

Also Published As

Publication number Publication date
US6113767A (en) 2000-09-05
WO1999060390A9 (en) 2000-02-24
WO1999060390A2 (en) 1999-11-25

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