WO1999067398A3 - Overexpression of phytase genes in yeast systems - Google Patents

Overexpression of phytase genes in yeast systems Download PDF

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Publication number
WO1999067398A3
WO1999067398A3 PCT/US1999/014106 US9914106W WO9967398A3 WO 1999067398 A3 WO1999067398 A3 WO 1999067398A3 US 9914106 W US9914106 W US 9914106W WO 9967398 A3 WO9967398 A3 WO 9967398A3
Authority
WO
WIPO (PCT)
Prior art keywords
phytase
overexpression
yeast systems
phytase genes
yeast
Prior art date
Application number
PCT/US1999/014106
Other languages
French (fr)
Other versions
WO1999067398A2 (en
WO1999067398A9 (en
Inventor
Xingen Lei
Original Assignee
Cornell Res Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22302276&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1999067398(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP99935340.2A priority Critical patent/EP1090129B2/en
Priority to CA2332180A priority patent/CA2332180C/en
Priority to DE69929886.5T priority patent/DE69929886C5/en
Priority to AU50837/99A priority patent/AU772071B2/en
Priority to DK99935340.2T priority patent/DK1090129T4/en
Priority to PL99345408A priority patent/PL345408A1/en
Priority to MXPA00012794 priority patent/MX258391B/en
Application filed by Cornell Res Foundation Inc filed Critical Cornell Res Foundation Inc
Priority to KR1020007014601A priority patent/KR100870886B1/en
Priority to ES99935340T priority patent/ES2255281T5/en
Priority to BRPI9911549A priority patent/BRPI9911549B1/en
Priority to HU0102800A priority patent/HUP0102800A2/en
Priority to JP2000556038A priority patent/JP2002518052A/en
Publication of WO1999067398A2 publication Critical patent/WO1999067398A2/en
Publication of WO1999067398A3 publication Critical patent/WO1999067398A3/en
Publication of WO1999067398A9 publication Critical patent/WO1999067398A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric

Abstract

The present invention relates to a method of producing a heterologous protein or polypeptide having phytase activity in a yeast system. The invention also provides proteins having phytase activity which have increased thermostability. Yeast strains which produce a heterologous phytase and the vectors used to produce the phytase are also provided.
PCT/US1999/014106 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems WO1999067398A2 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
JP2000556038A JP2002518052A (en) 1998-06-25 1999-06-23 Overexpression of phytase gene in yeast system
MXPA00012794 MX258391B (en) 1998-06-25 1999-06-23 OVEREXPRESSION OF FITASA GENES IN LIFTING SYSTEMS
DE69929886.5T DE69929886C5 (en) 1998-06-25 1999-06-23 OVEREXPRESSION OF PHYTASE IN YEAST SYSTEMS
AU50837/99A AU772071B2 (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems
KR1020007014601A KR100870886B1 (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems
PL99345408A PL345408A1 (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems
CA2332180A CA2332180C (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems
EP99935340.2A EP1090129B2 (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems
DK99935340.2T DK1090129T4 (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems
ES99935340T ES2255281T5 (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems
BRPI9911549A BRPI9911549B1 (en) 1998-06-25 1999-06-23 appa phytase production process of escherichia coli in yeast, protein or polypeptide, expression vector, and, yeast
HU0102800A HUP0102800A2 (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/104,769 1998-06-25
US09/104,769 US6451572B1 (en) 1998-06-25 1998-06-25 Overexpression of phytase genes in yeast systems

Publications (3)

Publication Number Publication Date
WO1999067398A2 WO1999067398A2 (en) 1999-12-29
WO1999067398A3 true WO1999067398A3 (en) 2000-04-20
WO1999067398A9 WO1999067398A9 (en) 2000-06-02

Family

ID=22302276

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/014106 WO1999067398A2 (en) 1998-06-25 1999-06-23 Overexpression of phytase genes in yeast systems

Country Status (18)

Country Link
US (6) US6451572B1 (en)
EP (3) EP1688500B1 (en)
JP (2) JP2002518052A (en)
KR (1) KR100870886B1 (en)
CN (1) CN100365126C (en)
AT (1) ATE317904T1 (en)
AU (1) AU772071B2 (en)
BR (1) BRPI9911549B1 (en)
CA (1) CA2332180C (en)
CZ (1) CZ301680B6 (en)
DE (1) DE69929886C5 (en)
DK (2) DK1688500T3 (en)
ES (2) ES2560661T3 (en)
HU (1) HUP0102800A2 (en)
MX (1) MX258391B (en)
PL (1) PL345408A1 (en)
PT (1) PT1688500E (en)
WO (1) WO1999067398A2 (en)

Cited By (2)

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US8455232B2 (en) 1998-06-25 2013-06-04 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
US8540984B2 (en) 2006-08-03 2013-09-24 Cornell Research Foundation, Inc. Phytases with improved thermal stability

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TW200305430A (en) * 2001-12-28 2003-11-01 Syngenta Participations Ag Thermotolerant phytase for animal feed
TWI262083B (en) * 2001-12-28 2006-09-21 Syngenta Participations Ag Microbially-expressed thermotolerant phytase for animal feed
CN100398645C (en) * 2002-08-16 2008-07-02 广东肇庆星湖生物科技股份有限公司 Yeast expression high specic activity phytase gene obtained using chemical synthesis and molecular evolution
CN100354421C (en) * 2002-08-16 2007-12-12 广东肇庆星湖生物科技股份有限公司 High expressed high temperature resistant phytase gene in methanol yeast
CA2925807C (en) 2002-09-13 2020-09-15 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
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WO2006012739A1 (en) * 2004-08-02 2006-02-09 UNIVERSITé LAVAL Nutritional ingredient containing bioavailable mineral nutrients
KR100754242B1 (en) 2005-08-29 2007-09-03 (주)진바이오텍 Method for Preparing Escherichia coli Phytase Using Promoter Derived from Yeast
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
EP2258854A1 (en) * 2009-05-20 2010-12-08 FH Campus Wien Eukaryotic host cell comprising an expression enhancer
EP2743347A2 (en) 2011-08-08 2014-06-18 Fertinagro Nutrientes, S.L. Novel phytase, method for obtaining same and use thereof
PL2964765T3 (en) * 2013-03-08 2019-10-31 Keck Graduate Inst Of Applied Life Sciences Yeast promoters from pichia pastoris
ES2716619T3 (en) 2013-03-08 2019-06-13 Biogrammatics Inc Yeast promoters for protein expression
CN110996667A (en) * 2017-07-27 2020-04-10 轨迹农业Ip有限责任公司 Efficient production of pichia pastoris and its use in promoting plant and animal health
EP3453719A1 (en) 2017-09-07 2019-03-13 Huvepharma Eood New thermostable phytases with high catalytic efficacy
CN109601822B (en) * 2019-01-22 2022-11-08 北京三强核力辐射工程技术有限公司 Method for degrading antibiotics in animal-derived food by synergistic irradiation of alkaloid
CN110029120B (en) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 Phytase high-yield strain and application thereof
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