WO2000001231A3 - Improved methods for storing neural cells - Google Patents

Improved methods for storing neural cells Download PDF

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Publication number
WO2000001231A3
WO2000001231A3 PCT/US1999/015115 US9915115W WO0001231A3 WO 2000001231 A3 WO2000001231 A3 WO 2000001231A3 US 9915115 W US9915115 W US 9915115W WO 0001231 A3 WO0001231 A3 WO 0001231A3
Authority
WO
WIPO (PCT)
Prior art keywords
neural cells
methods
storing
cells
improved methods
Prior art date
Application number
PCT/US1999/015115
Other languages
French (fr)
Other versions
WO2000001231A8 (en
WO2000001231A2 (en
Inventor
Jan Koopmans
Douglas B Jacoby
Jonathan Dinsmore
Original Assignee
Diacrin Inc
Jan Koopmans
Douglas B Jacoby
Jonathan Dinsmore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diacrin Inc, Jan Koopmans, Douglas B Jacoby, Jonathan Dinsmore filed Critical Diacrin Inc
Priority to AU49680/99A priority Critical patent/AU760659B2/en
Priority to EP99933678A priority patent/EP1094703A2/en
Priority to CA002333303A priority patent/CA2333303A1/en
Priority to US09/743,242 priority patent/US6821779B1/en
Publication of WO2000001231A2 publication Critical patent/WO2000001231A2/en
Publication of WO2000001231A3 publication Critical patent/WO2000001231A3/en
Publication of WO2000001231A8 publication Critical patent/WO2000001231A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The instant methods pertain to improved methods for storing neural cells, preferably dissociated neural cells, prior to their use in transplantation and to the cells obtained using such methods. One embodiment pertains to methods for storing the neural cells in medium lacking added buffer or added protein, other embodiments feature neural cells which are maintained at 4 °C prior to cryopreservation and have comparable viability and/or functionality to freshly harvested cells. In addition, methods for storing and/or transplantation of porcine neural cells are described.
PCT/US1999/015115 1998-07-06 1999-07-01 Improved methods for storing neural cells WO2000001231A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU49680/99A AU760659B2 (en) 1998-07-06 1999-07-01 Improved methods for storing neural cells
EP99933678A EP1094703A2 (en) 1998-07-06 1999-07-01 Improved methods for storing neural cells
CA002333303A CA2333303A1 (en) 1998-07-06 1999-07-01 Improved methods for storing neural cells
US09/743,242 US6821779B1 (en) 1998-07-06 1999-07-01 Methods for storing neural cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/110,772 1998-07-06
US09/110,772 US6713245B2 (en) 1998-07-06 1998-07-06 Methods for storing neural cells such that they are suitable for transplantation

Publications (3)

Publication Number Publication Date
WO2000001231A2 WO2000001231A2 (en) 2000-01-13
WO2000001231A3 true WO2000001231A3 (en) 2000-06-22
WO2000001231A8 WO2000001231A8 (en) 2000-08-03

Family

ID=22334848

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/015115 WO2000001231A2 (en) 1998-07-06 1999-07-01 Improved methods for storing neural cells

Country Status (5)

Country Link
US (2) US6713245B2 (en)
EP (1) EP1094703A2 (en)
AU (1) AU760659B2 (en)
CA (1) CA2333303A1 (en)
WO (1) WO2000001231A2 (en)

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US7311905B2 (en) * 2002-02-13 2007-12-25 Anthrogenesis Corporation Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells
CA2430989A1 (en) 2000-12-06 2002-06-13 Robert J. Hariri Method of collecting placental stem cells
WO2002063962A1 (en) 2001-02-14 2002-08-22 Hariri Robert J Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells
KR101042448B1 (en) * 2002-11-26 2011-06-16 안트로제네시스 코포레이션 Cytotherapeutics, cytotherapeutic units and methods for treatments using them
US20070269412A1 (en) 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
NZ597304A (en) * 2005-10-13 2013-06-28 Anthrogenesis Corp Immunomodulation using placental stem cells
EP2390312A1 (en) 2005-11-29 2011-11-30 Gamida Cell Ltd. Methods of improving stem cell homing and engraftment
JP5550235B2 (en) 2005-12-29 2014-07-16 アントフロゲネシス コーポレーション Placental stem cell population
AU2006332679A1 (en) 2005-12-29 2007-07-12 Anthrogenesis Corporation Co-culture of placental stem cells and stem cells from a second source
CN104099290A (en) 2006-10-23 2014-10-15 人类起源公司 Methods and compositions for treatment of bone defects with placental cell populations
CN103356711A (en) 2007-02-12 2013-10-23 人类起源公司 Immunomodulation using placental stem cells
AU2008307633C1 (en) 2007-09-28 2015-04-30 Celularity Inc. Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells
JP6169316B2 (en) 2008-08-20 2017-07-26 アンスロジェネシス コーポレーション Improved cell composition and method for producing the same
KR20180108887A (en) 2008-08-20 2018-10-04 안트로제네시스 코포레이션 Treatment of stroke using isolated placental cells
RU2562154C2 (en) 2008-11-19 2015-09-10 Антродженезис Корпорейшн Amniotic adhesive cells
US20100209399A1 (en) * 2009-02-13 2010-08-19 Celavie Biosciences, Llc Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects
ES2646750T3 (en) 2010-01-26 2017-12-15 Anthrogenesis Corporation Treatment of bone-related cancers using placental stem cells
KR20190076060A (en) 2010-04-07 2019-07-01 안트로제네시스 코포레이션 Angiogenesis using placental stem cells
EP2555783A1 (en) 2010-04-08 2013-02-13 Anthrogenesis Corporation Treatment of sarcoidosis using placental stem cells
CN101971796B (en) * 2010-05-26 2013-04-17 赛业（广州）生物科技有限公司 Nonprogrammed cell frozen stock solution free of proteins
WO2012009422A1 (en) 2010-07-13 2012-01-19 Anthrogenesis Corporation Methods of generating natural killer cells
US8969315B2 (en) 2010-12-31 2015-03-03 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
ES2707579T3 (en) 2011-06-01 2019-04-04 Celularity Inc Pain treatment using placental cytoblast
WO2013055476A1 (en) 2011-09-09 2013-04-18 Anthrogenesis Corporation Treatment of amyotrophic lateral sclerosis using placental stem cells
JP6012164B2 (en) * 2011-11-25 2016-10-25 住友化学株式会社 Method for cryopreserving tissue derived from pluripotent stem cells
AU2014215458A1 (en) 2013-02-05 2015-08-13 Anthrogenesis Corporation Natural killer cells from placenta
US10634686B2 (en) * 2013-09-23 2020-04-28 University of Pittsburgh—of the Commonwealth System of Higher Education Biomarkers related to organ function

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1329499A3 (en) * 1991-07-08 2003-09-03 Neurospheres Holdings Ltd. Growth factor-responsive progenitor cells which can be proliferated in vitro

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
C. HUMPEL ET AL.: "HUMAN FETAL CORTICAL TISSUE FRAGMENTS SURVIVE GRAFTING FOLLOWING ONE WEEK TORAGE AT +4°C", CELL TRANSPLANTATION, vol. 3, no. 6, 1994, NEW YORK, US, pages 475 - 479, XP000867331 *
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; CAI R S ET AL: "Cryopreservation and culture of the human fetal brain tissues.", XP002128374, retrieved from STN Database accession no. 94125473 *
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; YOSHIMOTO Y ET AL: "Cool storage of fetal rat brain for neural transplantation.", XP002128373, retrieved from STN Database accession no. 93001643 *
G. NIKKHAH ET AL.: "PRESERVATION OF FETAL VENTRAL MESENCEPHALIC CELLS BY COOL ATORAGE: IN-VITRO VIABILITY AND TH-POSITIVE NEURON SURVIVALAFTERR MICROTRANSPLANTATION TO THE STRIATUM.", BRAIN RESEARCH, vol. 687, no. 1-2, 1995, AMSTERDAM,NL, pages 22 - 34, XP000867281 *
J.C. KAWAMOTO ET AL.: "CRYOPRESERVATION OF PRIMARY NEURONS FOR TISSUE CULTURE.", BRAIN RESEARCH, vol. 384, no. 1, 1986, AMSTERDAM, NL, pages 84 - 93, XP000867273 *
JOURNAL OF TONGJI MEDICAL UNIVERSITY, (1993) 13 (3) 138-42. *
NO TO SHINKEI. BRAIN AND NERVE, (1992 JUN) 44 (6) 553-7. *
P. BRUNDIN ET AL.: "MONITORING OF CELL VIABILITY IN SUSPENSIONS OF EMBRYONIC CNS TISSUE AND ITS USE AS A CRITERION FOR INTRACEREBRAL GRAFT SURVIVAL.", BRAIN RESEARCH, vol. 331, no. 2, 1985, AMSTERDAM, NL, pages 251 - 259, XP000867410 *
V. SILANI ET AL.: "HUMAN NEURONAL CELL VIABILITY DEMONSTRATED IN CULTURE AFTER CRYOPRESERVATION.", BRAIN RESEARCH, vol. 473, no. 1, 1988, AMSTERDAM, NL, pages 169 - 174, XP000867327 *

Also Published As

Publication number Publication date
WO2000001231A8 (en) 2000-08-03
AU760659B2 (en) 2003-05-22
CA2333303A1 (en) 2000-01-13
EP1094703A2 (en) 2001-05-02
AU4968099A (en) 2000-01-24
US6713245B2 (en) 2004-03-30
US6821779B1 (en) 2004-11-23
WO2000001231A2 (en) 2000-01-13
US20020102239A1 (en) 2002-08-01

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