WO2000003222A3 - Method for fabricating biosensors using hydrophilic polyurethane - Google Patents

Method for fabricating biosensors using hydrophilic polyurethane Download PDF

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Publication number
WO2000003222A3
WO2000003222A3 PCT/KR1999/000368 KR9900368W WO0003222A3 WO 2000003222 A3 WO2000003222 A3 WO 2000003222A3 KR 9900368 W KR9900368 W KR 9900368W WO 0003222 A3 WO0003222 A3 WO 0003222A3
Authority
WO
WIPO (PCT)
Prior art keywords
biosensors
bio
hydrophilic polyurethane
fabricating
active
Prior art date
Application number
PCT/KR1999/000368
Other languages
French (fr)
Other versions
WO2000003222A2 (en
Inventor
Geun Sig Cha
Hakhyun Nam
Jae Ho Shin
Original Assignee
Geun Sig Cha
Hakhyun Nam
Jae Ho Shin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geun Sig Cha, Hakhyun Nam, Jae Ho Shin filed Critical Geun Sig Cha
Priority to US09/485,513 priority Critical patent/US6509148B2/en
Publication of WO2000003222A2 publication Critical patent/WO2000003222A2/en
Publication of WO2000003222A3 publication Critical patent/WO2000003222A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes

Abstract

The present invention relates to the method for fabricating biosensors comprising signal detector and signal transducer. In particular, the present invention relates to the method for fabricating biosensors by mixing bio-active reagents, including enzymes, antibodies, antigens, cells and receptors, with hydrophilic polyurethane and then coating a signal transducer with the mixture directly to form a sensing film which serves as a signal detector. The method using hydrophilic polyurethane allows the simplification of the fabrication of biosensors without conducting complicated chemical reactions and washing steps, such as cross-linking. The bio-active reagent entrapped within the hydrophilic polyurethane film can retain its high activity for an extended period of time and the intrinsic potentiometric response of the underlying ion-selective polymeric membrane is not affected by the bio-active reagent immobilized polyurethane film coated on its sensing surface. Therefore, the biosensors are superior in specificity, selectivity, and stability. Also, the adoption of solid-state type electrodes by virtue of the direct immobilization of the bio-active reagents allows the biosensors to be miniaturized and to be mass-produced for low cost.
PCT/KR1999/000368 1998-07-09 1999-07-09 Method for fabricating biosensors using hydrophilic polyurethane WO2000003222A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US09/485,513 US6509148B2 (en) 1998-07-09 1999-07-09 Method for fabricating biosensors using hydrophilic polyurethane

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-1998-0027563A KR100349000B1 (en) 1998-07-09 1998-07-09 Manufacturing method of biosensor using hydrophilic polyurethane
KR1998/27563 1998-07-09

Publications (2)

Publication Number Publication Date
WO2000003222A2 WO2000003222A2 (en) 2000-01-20
WO2000003222A3 true WO2000003222A3 (en) 2000-03-30

Family

ID=19543529

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR1999/000368 WO2000003222A2 (en) 1998-07-09 1999-07-09 Method for fabricating biosensors using hydrophilic polyurethane

Country Status (3)

Country Link
US (1) US6509148B2 (en)
KR (1) KR100349000B1 (en)
WO (1) WO2000003222A2 (en)

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KR100455907B1 (en) * 2001-07-28 2004-11-12 주식회사 아이센스 Non-separation type enzyme-immunsensor using parallel microporous electrodes
WO2002097416A1 (en) * 2001-05-30 2002-12-05 I-Sens, Inc. Biosensor
US6896778B2 (en) * 2001-06-04 2005-05-24 Epocal Inc. Electrode module
JP4385219B2 (en) 2001-09-14 2009-12-16 アークレイ株式会社 Concentration measurement method
US7094330B2 (en) * 2002-12-02 2006-08-22 Epocal Inc. Heterogeneous membrane electrodes
US7842234B2 (en) 2002-12-02 2010-11-30 Epocal Inc. Diagnostic devices incorporating fluidics and methods of manufacture
US7767068B2 (en) 2002-12-02 2010-08-03 Epocal Inc. Heterogeneous membrane electrodes
EP1591778A1 (en) * 2004-04-26 2005-11-02 Roche Diagnostics GmbH Electrochemical gas sensor with hydrophilic membrane coating
US8394338B2 (en) * 2004-04-26 2013-03-12 Roche Diagnostics Operations, Inc. Process for hydrophilizing surfaces of fluidic components and systems
EP1885871B1 (en) 2005-05-17 2012-05-30 Radiometer Medical ApS Enzyme sensor with a cover membrane layer covered by a hydrophilic polymer
US9700252B2 (en) 2006-06-19 2017-07-11 Roche Diabetes Care, Inc. Amperometric sensor and method for its manufacturing
CN101473225B (en) * 2006-06-19 2016-05-11 霍夫曼-拉罗奇有限公司 Amperometric sensor and manufacture method thereof
US7751864B2 (en) 2007-03-01 2010-07-06 Roche Diagnostics Operations, Inc. System and method for operating an electrochemical analyte sensor
KR100928202B1 (en) * 2007-12-10 2009-11-25 한국전자통신연구원 Silicon biosensor and its manufacturing method
KR101050695B1 (en) 2008-12-15 2011-07-20 한국전자통신연구원 Manufacturing method of transistor for biosensor
US9084570B2 (en) 2010-10-08 2015-07-21 Roche Diagnostics Operations, Inc. Electrochemical sensor having symmetrically distributed analyte sensitive areas
US11125737B2 (en) * 2016-12-16 2021-09-21 Siemens Healthcare Diagnostics Inc. Microcapillary sensor array
CN110327056B (en) * 2019-05-24 2021-01-01 武汉大学 Intelligent hydrogel for visual blood glucose detection and preparation method thereof
KR20210041191A (en) 2019-10-07 2021-04-15 김용식 Device for cutting steel plate

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WO1996030431A1 (en) * 1995-03-27 1996-10-03 Minimed Inc. Polyurethane/polyurea compositions containing silicone for biosensor membranes

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WO1996030431A1 (en) * 1995-03-27 1996-10-03 Minimed Inc. Polyurethane/polyurea compositions containing silicone for biosensor membranes

Also Published As

Publication number Publication date
KR20000007958A (en) 2000-02-07
KR100349000B1 (en) 2003-03-26
WO2000003222A2 (en) 2000-01-20
US6509148B2 (en) 2003-01-21
US20020107227A1 (en) 2002-08-08

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