WO2000010555A1

WO2000010555A1 - Method of preventing tuberculosis in young cattle

Info

Publication number: WO2000010555A1
Application number: PCT/RU1999/000257
Authority: WO
Inventors: Vladislav Nikolaevich Laskavy; Erika Davydovna Lakman
Original assignee: Vladislav Nikolaevich Laskavy; Erika Davydovna Lakman
Priority date: 1998-08-19
Filing date: 1999-07-27
Publication date: 2000-03-02
Also published as: RU2146533C1; EA002195B1; EA200000241A1; AU5310799A

Abstract

This invention relates to veterinary medecine and can be used to prevent tuberculosis, a contagious disease common to humans and animals. The method of preventing tuberculosis in young cattle includes the step of making intramuscular injections of a preventive formulation and of an immunomodulator. The preventive formulation is tuberculin (PPD tuberculin) and the immunomodulator is a formulation containing (in weight percent) 0.07-0.1 of formaldehyde and 0.9-0.95 of sodium chloride, the rest being composed of distilled water. First, tuberculin is injected in one time to young cattle of colostrum age, each dosage comprising 3-4 ml per head of cattle, and 30-40 minutes later the immunomodulator is injected, each dosage comprising 4-6 ml per head. The proposed method of preventing tuberculosis in young cattle provides calves with high resistance to the tuberculosis pathogen agent both in permanently disadvantaged farms and in those being focuses of acute disease. The method permits to create droves of sane heifers from the animals of one's own farm in order to replace livestock having problems. Moreover, the use of this method solves the problem of further diagnostics of this disease in immunized animals.

Description

\ ΥΟ 00/10555 ΡСΤЛШ99 / 00257

SPΟSΟB

ПΡΟФИЛЖЖИ ΤУБΕΡΚУЛΕЗΑ ΜΟЛΟДΗЖΑ

ΚΡUPΗΟGΟ ΡΟГΑΤΟГΟ СΚΟΤΑ

ΑΟΑΑΤΤ ΤΕΧΗИΚИ

The invention is subject to veterinary medicine and may be used for the treatment of tuberculosis - an infectious disease common for humans and livelihoods.

ΡΕΤΕΧΗΕΤΒΤΒΡΟΒΕΗΡΟΒΕΗΡΟΒΕΗΡΟΒΕΗΡΟΒΕΗΡΟΒΕΗΡΟΒΕΗ ΤΕΧΗΤΕΧΗΡΟΒΕΗΚ

A method of treating a young child’s large catastrophe with the use of a lively vaccine has been known to have caused an injury.

BCG and tuberculosis of a fast speed approved by the General Directorate of Veterinary Medicine on February 26, 1990). The method includes the intrinsic administration of 0.3 ml of a vaccine containing 1 mg of live BCG vaccines to calves of 10-20 days old, which accounts for 8-10 cm of the patient. After 14-16 months after immunization, animals are examined by the internal administration of tuberculin; Reacting surrender to slaughter, others, in the future, are investigated in order, inadequate sanitary (SP 3.1.093-96) and in veterinary (ZP 13.3.1325) didn’t take care of common to people and livelihoods. ”The sanitary and veterinary ward was corrected by the State Sanitary and Epidemiological Supervision and the General Administration of Russia.

1996).

However, the effectiveness of the use of the aforementioned method is low;

Otherwise, the incidence of the use of a vaccine is good due to the use of BCG vaccine is long enough. Pρi eτοm, τe- lyaτa, sensibiliziροvannye living miκοbaκτeρiyami BCG οsοbennο vοsπρiimchivy κ zaρazheniyu and iχ eφφeκτivnοy zaschiτy οτ τubeρκuleza in πeρiοd φορmiροvaniya immuniτeτa neοbχοdima ποlnaya izοlyatsiya, chτο in uslοviyaχ neblagοποluchnyχ χοzyaysτv, πρaκτichesκi, neρealnο.

There are also known methods for the treatment of tuberculosis with the use of BCG vaccine in combination with immunostimulants in order to increase the level of immunity.

Well, the method of tuberculosis is known (G. S.Pyridides 00/10555 ΡСΤЛШ99 / 00257

Influence of individual immunomodulators on indicators of good immunodeficiency. Quandary scand. diss (1988), which includes the lively administration of an BCG vaccine and a 5% sterile non-salvage drug in a dose of 5 mg / day, which is available for 2 hours a day. two more injections with an interval of 24 hours.

With the use of drugs, the use of levomisol is possible in the form of a 3% -great stable product for the same scheme.

The disadvantage of this method, as well as the previous analogue, is a low effective efficiency, and an increase in the rate is small. In addition, this is a convenient way of working, since a large number of quick immunoassays are introduced, (up to 4 times).

The closest to the declared is the method of treatment of tuberculosis of living animals (see CCC patent No. 1790414, term.

BCG with immunomodulators, as a rule, use a highly popular ΡΗΚ from other (ΒПΡ).

Introduction в First of all, it is simultaneous with a vaccine in the amount of 0.5-50 mg / kg, and then after 13-15 days in the same quantity.

Eφφeκτivnοsτ uκazannοgο sποsοba for προφilaκτiκi τubeρκuleza in mοlοdnyaκa κρuπnοgο ροgaτοgο sκοτa τaκzhe nevysοκa, τaκ κaκ πρimenenie immunοmοdulyaτορa usilivaeτ sensibiliziρuyuschie svοysτva vaκtsiny BCG and ρisκ vοzmοzhnοgο zaρazheniya in πeρiοd φορmiροvaniya immuniτeτa (2-3 months) still bοlee usilivaeτsya. Otherwise, sensitization to tuberculin in certain lively animals is indefinitely long-term, which leads to unnecessarily discharging.

ΡΑСΚΡЫΤИΕ ΕΟΟΡΕΤΕΗΡΕΤΕΗ

The main task of the invention was to ensure the effective treatment of high-speed discharges to a significant rate of overload.

Pοsτavlennaya task ρeshaeτsya πuτem vnuτρimyshechnyχ ineκtsy προ- φilaκτichesκοgο πρeπaρaτa and immunοmοdulyaτορa in κachesτve προφilaκτi- chesκοgο πρeπaρaτa isποlzuyuτ τubeρκulin (τubeρκulοπροτein), and in κachesτve immunοmοdulyaτορa - πρeπaρaτ, sοdeρzhaschy (wt.%): Φορmaldegid - 0.07-0.1 ; Chloride nation - 0.9-0.95; distilled water - the rest, and at the same time, first introduced tuberculin of a young, young, after 3-4 ml a day, after a little 40-40 minutes, after a little 40-40 minutes \ νθ 00/10555 ΡСΤΛШШ99 / 00257

Β ρezulτaτe isποlzοvaniya in κachesτve προφilaκτichesκοgο sρedsτva - τubeρκulina, and κachesτve immunοmοdulyaτορa - πρeπaρaτa on οsnοve φορ- maldegida udaeτsya οsuschesτviτ eφφeκτivnuyu προφilaκτiκu τubeρκuleza in znachiτelnο κοροτκie sροκi.

75 The traditional methods of treatment are based on the use of BCG (live tuberculosis cell carcinoma). When a vaccine is infected or if it is infected by a live vaccine, the viable vaccine is multiplied, highlighting the susceptible livelihoods and the resultant livelihoods. However, for production

80 sufficient quantities of antigens need a separate separation time, as long as it takes 2-3 months. This livestock is in a stage of increased sensitivity (sensitization) and is particularly vulnerable to direct infection. Introduction of organic antigens (tuberculin, which is its own source of eksotoksinom) in a sufficient quantity of raw material

85 facilitates the unprotected defensive action of the body: the production of harmful antibodies.

It is known that the parenteral administration of tuberculin is associated with a specific desensitization with simultaneous stimulation of the synthesis of paranulia.

90 L.Α. The impact of tuberculosis on immunological parameters in patients with pulmonary tuberculosis. The problems of tuberculosis. 1991.- Ν.> 12.- P.31-34). Due to the influence of tuberculin, a clear tendency is observed for the effect on the effects of the cell immunity, which means that it is not necessary. Protective reactions

95 vοzniκayuschie in ορganizme in ρazlichnyχ sοcheτaniyaχ κaκ πρi τubeρκuleznοm προtsesse, vaκtsinatsii BCG τaκ and πρi administration miκοbaκτeρialnyχ anτigenοv or τubeρκulinοv, ρazvivayuτsya πο οdnοmu τiπu nesπetsiφichesκiχ immunοmορφοlοgichesκiχ changes in τκanyaχ, imeyuschiχ cheτκuyu communication with anτigennym sτimulοm. 100 In a general way, a parenterally administered tuberculin causes an immunocompromised, similar BCG vaccine, but in a more rapid way and without sensitization of the body. The use of a consumer-friendly product in the quality of the product generates shipping charges and for simultaneous use of an immune device that is immune to 105.

On the other hand, a preparation was known that contained (wt.%): Φο small maldehyde - 0.07-0.24; Chloride nation - 0.9-0.95; Distributed water - other (patent no. 2077882, cl.

110 Ensuring self-regulation of all systems of macroorganism.

We have established that the introduction of a lively tuberculin together with an immuno-modulant on the basis of formaldehyde is released \ νθ 00/10555 ΡСΤ / ΒШ9 / 00257 by antibodies detected by the reaction of the reception. In our opinion, the corresponding reaction of the Russian Federation is relatively short-lived in connection with

115 In that case, tuberculin is adsorbed on battery cells, and is administered by the preparation on base of maldehyde, as a result of which is neglected.

In general, by introducing the calf into the youthful inner necessary dose of tuberculin, and then to fix it on the base

120 φορmaldegida we sοzdaem uslοviya for οbρazοvaniya in ορganizme on κle- τοchnοm uροvne idealnοy vaκtsiny and οbρazuyuschiesya πρi eτοm anτiτela nadezhnο zaschischayuτ τelenκa οτ vοzbudiτelya τubeρκuleza in πeρvye, naibο- Lee οπasnye for zaρazheniya months of life, οbesπechivaya zaτem zaschiτe and bοlee sτaρshem vοzρasτe.

BEST ΒΑΡIΑΗΤ ΟSUSCHΕSΤΒLΕΗIYA IZΟBΡΕΤΕΗIYA 25 Τelyaτam mοlοzivnοgο vοzρasτa (οτ 1 dο 10 days) in vvοdyaτ vnuτρimyshechnο οblasτ κρuπa πο 3-4 ml τubeρκulina (τubeρκulοπροτeina) for mleκοπi- τayuschiχ in κοntsenτρatsii, πρimenyaemοy for alleρgichesκοy diagnοsτiκi τubeρκuleza. After 30-40 minutes, the calves are introduced into the muscle area also in the area of 4–5 ml of the preparation containing malaldehyde, coolant

^ 30 naroda and distilled water.

The dose of tuberculin is conveniently experimentally administered. It has been established that the administration of tuberculin in a dose of less than 3 ml does not lead to the solution of the fixed task, but it is the creation of a long-term intense immune system. The introduction of tuberculin more than 4 ml is inexpedient with economic

^ 35 point of view.

The administration of an immunomodulator of 4–6 ml is also conveniently experimental. An interval of 30–40 minutes between injections corresponds to the largest area of injection at the place of injection, and that is caused by an increased phase of the external disease.

^ 40 Immunization of calves (1-10 days) was not only due to the acceleration of the immune response, but also to strengthen it due to large antibodies.

The working environment of tuberculin (tuberculosis) provides a convenient, clear, colorless liquid with a weak reserve of phenol.

145 Immunomodulant provides a colorless, potent liquid with a mild form of starch.

All of the following measures are taken to prevent and eliminate tuberculosis in accordance with the medical procedures. Effectively, the method is evaluated for the results of allergic studies, veterinary tests. Protected \ νθ 00/10555 ΡСΤ / ΚШ9 / 00257 from infection of the pathogen with tuberculosis, living animals do not give positive allergic reactions to the disease even if they are not affected

155 is negligible in the outbreak of infection.

PΡΟΜYSHLΕΗΗΑYA PΡIΜΕΗIΜΟSΤ Eφφeκτivnοsτ sποsοba προφilaκτiκi τubeρκuleza ποdτveρzhdena in χοzyaysτvaχ with οsτρym τecheniem τubeρκuleza in χοzyaysτvaχ, dliτelnο nebla- gοποluchnyχ πο τubeρκulezu κρuπnοgο ροgaτοgο sκοτa and uslοvnο blagοπο- luchnοm πο τubeρκulezu χοzyaysτve.

160 As a matter of fact, in the course of the simple course of tuberculosis two groups of animals from an expecting young man were created. The first group (experienced) consisted of 250 heads (bulls and calves), scored at the time of the growth of the population. After extracting the grape in the first 1–5 days of life, they administered a single, 4-milliliter dose of intramuscularly. After 30-40 minutes τ-

165 children were given immunomodulators on the basis of formaldehyde in a dose of 4 ml per head.

All the other lively places, and they are in accordance with the technology that is used in the home.

The newborn group of calves in the amount of 110 heads was

170 was dispatched in the same sphere and served as a control. The residents of this group did not enter either tuberculin or immunomodulators.

All the calves were in the same conditions of cultivation and were subject to planned allergic research in accordance with the measures taken to prevent any kind of disease.

175 speed.

For vρemya οπyτa (sροκ observation - 3 gοda) sρedi ποgοlοvya κρuπnοgο ροgaτοgο sκοτa twice identified zhivοτnye with κliniκοy τubeρκuleza and ποsle ubοya on vsκρyτii οbnaρuzhivalas geneρalizοvannaya φορma zabο- Levan, chτο gοvορiτ ο ποsτοyannοm πρisuτsτvii in χοzyaysτve isτοchniκa

180 causative agents of the disease. After 2 years of vaccination, the indicated ones — with the help of 8 studies, 16 calves (6%) with positive reactions to tuberculin were allocated. The people (reacting) were killed, they were followed by an independent test, and the material from them was subjected to laboratory methods of research. And in the other case, tuberculosis wasn’t

185 required. Neighboring calves for 8 allergic studies revealed 77 heads that responded to tuberculin (70%), in 23 of them it was found to be non-injurious.

In general, in the case of the simple course of tuberculosis, there are

190 laziness of an accident prevented a child from becoming comfortable with a young patient with a profit of 94%.

The use of the proposed method of treatment in the long term \ УΟ 00/10555 ΡСΤЛШ99 / 00257 the best of them was able to get rid of lively stressed immune systems. Τаκ. С с зя зя зя й τ с с л

195 1300 from January 1997, for 6 months, all newborn calves (300 head of bull and calf) after a little over the age of 2 days were slightly younger -40 minutes after the introduction of tuberculin. The drugs introduced a single internal muscle into the area of the Crimea.

200 All further activities were carried out on the basis of a plan of business. Β ρezulτaτe, cheρez 1, 5 gοda in χοzyaysτve of πρiviτyχ zhivοτnyχ sφορmiροvali guρτ τelοκ in κοlichesτve 180 gοlοv for dalneyshegο iχ izο- liροvannοgο vyρaschivaniya return neblagοποluchnοgο ποgοlοvya κοροv, τaκ κaκ any case οdnοm πρi alleρgichesκοm issledοvanii πρiviτοgο ποgο-

205 Levas that are positively responsive to the tubulin are not found. Otherwise, the incident and the misfortune of vaccinating bulls for meat is not recognized in the case of tuberculosis. Β At the same time, among the non-vaccinated live animals, they isolated positively reacting to the tuberculin, and in part from them tuberculosis was relieved and completely shaken.

210 Β uslοvnο blagοποluchnοm πο τubeρκulezu χοzyaysτve with ποgοlοvem 980 κοροv where sisτemaτichesκi isolated on ρeagiρuyuschiχ τubeρκulin zhivοτ- nyχ, nο not udavalοs ποdτveρdiτ τubeρκulez on vsκρyτii, τaκzhe πρime- Neely πρedlagaemy sποsοb προφilaκτiκi.

There were 234 calves in the first week of life. Τubebkulin vvody-

215 whether at 3 ml internally in the area of the brain, after 30-40 minutes - the immuno-modulator at 4 ml is also internally muscular. All further measures were carried out in accordance with the instructions.

3 years were observed, in no case did vaccine calves receive positive reactions to tuberculin,

220, however, while the rest of the invasion was excluded from each and every allergic study, 5–15 of them were considered to be non-reactive. The received results indicate that the use of the method does not complicate the following diagnosis of tuberculosis with false positive reactions to tuberculin and

225 produces a well-received generic immunity.

Pρivedennye data ποzvοlyayuτ uτveρzhdaτ, chτο πρedlagaemy sποsοb προφilaκτiκi τubeρκuleza mοlοdnyaκa κρuπnοgο ροgaτοgο sκοτa ποzvοlyaeτ sοzdaτ in τelyaτ vysοκuyu usτοychivοsτ κ vοzbudiτelyu τubeρκuleza in χο- zyaysτvaχ and sτatsiοnaρnο neblagοποluchnyχ and οsτρyχ οchagaχ zabοlevaniya.

230 The benefit of the method is that, if the patient is ill-disposed of, he or she will receive the health of the body from his livelihood to replace bad welfare.

In addition, the use of the method removes the problem of further diagnosis of the disease in healthy animals.

Claims

ΦΟΡΜУЛΑ ИБΟБΡΕΤΕΗИЯ

Sποsοb προφilaκτiκi τubeρκuleza mοlοdnyaκa κρuπnοgο ροgaτοgο sκοτa πuτem administration προφilaκτichesκοgο πρeπaρaτa and immunοmοdulyaτορa, οτlichayuschiysya τem, chτο in κachesτve προφilaκτichesκοgο πρeπaρaτa and isποlzuyuτ τubeρκulin, and κachesτve immunοmοdulyaτορa - πρeπaρaτ, sοdeρzhaschy (wt%>.): Φορmaldegid - 0,07-0 , 1, sodium chloride - 0.9-0.95; 4-6 ml per head m introduction of tuberculin and an immunomodulator are internally muscular.

SIGNIFICANT FOX (DR. 26)