WO2000022409A3 - Microfluidic devices connected to capillaries with minimal dead volume - Google Patents

Microfluidic devices connected to capillaries with minimal dead volume Download PDF

Info

Publication number
WO2000022409A3
WO2000022409A3 PCT/CA1999/000868 CA9900868W WO0022409A3 WO 2000022409 A3 WO2000022409 A3 WO 2000022409A3 CA 9900868 W CA9900868 W CA 9900868W WO 0022409 A3 WO0022409 A3 WO 0022409A3
Authority
WO
WIPO (PCT)
Prior art keywords
capillary
dead volume
capillaries
hole
devices connected
Prior art date
Application number
PCT/CA1999/000868
Other languages
French (fr)
Other versions
WO2000022409A2 (en
Inventor
Thompson Tang
D Jed Harrison
Nicolas Bings
Can Wang
Gregor Ocvirk
Jianjun Li
Cameron Skinner
Pierre Thibault
Original Assignee
Univ Alberta
Inst Biolog Sciences
Thompson Tang
D Jed Harrison
Nicolas Bings
Can Wang
Gregor Ocvirk
Jianjun Li
Cameron Skinner
Pierre Thibault
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Alberta, Inst Biolog Sciences, Thompson Tang, D Jed Harrison, Nicolas Bings, Can Wang, Gregor Ocvirk, Jianjun Li, Cameron Skinner, Pierre Thibault filed Critical Univ Alberta
Priority to CA002386056A priority Critical patent/CA2386056A1/en
Priority to AT99944204T priority patent/ATE267393T1/en
Priority to DE69917476T priority patent/DE69917476T2/en
Priority to EP99944204A priority patent/EP1127258B1/en
Publication of WO2000022409A2 publication Critical patent/WO2000022409A2/en
Publication of WO2000022409A3 publication Critical patent/WO2000022409A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6034—Construction of the column joining multiple columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Abstract

A method is provided for joining a microchip device to a capillary tube. The microchip device has a capillary channel opening onto an edge surface of the device. A short hole is drilled into the edge surface, aligned with the capillary channel. The drilling is done with a flat bottom, preferably by a two-step drilling process. Then, the end of the capillary can be inserted into the hole so that its end is substantially flush with the flat bottom of the hole, thereby eliminating dead volume. Testing has shown that this connection provides very little band broadening of samples transported through the capillary channel into the capillary tube. The tip of the capillary tube can be tapered, so that it is suitable for use as an electrospray source for a mass spectrometer.
PCT/CA1999/000868 1998-10-09 1999-09-23 Microfluidic devices connected to capillaries with minimal dead volume WO2000022409A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002386056A CA2386056A1 (en) 1998-10-09 1999-09-23 Microfluidic devices connected to capillaries with minimal dead volume
AT99944204T ATE267393T1 (en) 1998-10-09 1999-09-23 METHOD FOR CONNECTING A CAPILLARY TO A MICROFLUID DEVICE
DE69917476T DE69917476T2 (en) 1998-10-09 1999-09-23 METHOD FOR CONNECTING A CAPILLARY TO A MICROFLUID DEVICE
EP99944204A EP1127258B1 (en) 1998-10-09 1999-09-23 Method of joining a capillary tube to a microfluidic device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/169,146 US6605472B1 (en) 1998-10-09 1998-10-09 Microfluidic devices connected to glass capillaries with minimal dead volume
US09/169,146 1998-10-09

Publications (2)

Publication Number Publication Date
WO2000022409A2 WO2000022409A2 (en) 2000-04-20
WO2000022409A3 true WO2000022409A3 (en) 2000-07-13

Family

ID=22614404

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1999/000868 WO2000022409A2 (en) 1998-10-09 1999-09-23 Microfluidic devices connected to capillaries with minimal dead volume

Country Status (6)

Country Link
US (2) US6605472B1 (en)
EP (1) EP1127258B1 (en)
AT (1) ATE267393T1 (en)
CA (1) CA2386056A1 (en)
DE (1) DE69917476T2 (en)
WO (1) WO2000022409A2 (en)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1255986A1 (en) * 2000-02-18 2002-11-13 Evotec OAI AG An end element for capillaries or chip channels
EP1336097A4 (en) * 2000-10-13 2006-02-01 Fluidigm Corp Microfluidic device based sample injection system for analytical devices
DE10154601B4 (en) * 2000-11-13 2007-02-22 Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto A micro device with an integrated protruding electrospray emitter and a method of fabricating the micro device
US20020168780A1 (en) * 2001-02-09 2002-11-14 Shaorong Liu Method and apparatus for sample injection in microfabricated devices
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
EP1270073B1 (en) * 2001-06-28 2005-02-16 Agilent Technologies, Inc. (a Delaware corporation) Microfluidic system with controller
US6803568B2 (en) * 2001-09-19 2004-10-12 Predicant Biosciences, Inc. Multi-channel microfluidic chip for electrospray ionization
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US7105810B2 (en) * 2001-12-21 2006-09-12 Cornell Research Foundation, Inc. Electrospray emitter for microfluidic channel
DE10213390C1 (en) * 2002-03-26 2003-08-14 Bruker Daltonik Gmbh Combining electrophoresis with analysis by mass spectrometry comprises maintaining electrophoresis potential until desired substance has been separated and switching it off during analysis
JP2005521425A (en) 2002-04-01 2005-07-21 フルイディグム コーポレイション Microfluidic particle analysis system
US7143785B2 (en) 2002-09-25 2006-12-05 California Institute Of Technology Microfluidic large scale integration
EP1546412B1 (en) 2002-10-02 2014-05-21 California Institute Of Technology Microfluidic nucleic acid analysis
AU2003900329A0 (en) * 2003-01-24 2003-02-13 Microtechnology Centre Management Limited Microfluidic connector
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
CA2521171C (en) 2003-04-03 2013-05-28 Fluidigm Corp. Microfluidic devices and methods of using same
US7476363B2 (en) 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US7007710B2 (en) 2003-04-21 2006-03-07 Predicant Biosciences, Inc. Microfluidic devices and methods
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7537807B2 (en) 2003-09-26 2009-05-26 Cornell University Scanned source oriented nanofiber formation
US7282705B2 (en) 2003-12-19 2007-10-16 Agilent Technologies, Inc. Microdevice having an annular lining for producing an electrospray emitter
AU2005231431B2 (en) * 2004-04-02 2011-02-10 Eksigent Technologies Llc Microfluidic connections
US20060022130A1 (en) * 2004-07-29 2006-02-02 Predicant Biosciences, Inc., A Delaware Corporation Microfluidic devices and methods with integrated electrical contact
US7591883B2 (en) * 2004-09-27 2009-09-22 Cornell Research Foundation, Inc. Microfiber supported nanofiber membrane
US20060166347A1 (en) * 2005-01-27 2006-07-27 Applera Corporation Sample preparation devices and methods
JP2007043042A (en) * 2005-07-07 2007-02-15 Sumitomo Electric Ind Ltd Wafer holder and manufacturing method thereof, wafer prober mounting same, and semiconductor heating device
DE102005032983B4 (en) * 2005-07-14 2007-05-31 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. nozzle assembly
DK1957794T3 (en) 2005-11-23 2014-08-11 Eksigent Technologies Llc Electrokinetic pump designs and drug delivery systems
US20070170056A1 (en) * 2006-01-26 2007-07-26 Arnold Don W Microscale electrochemical cell and methods incorporating the cell
US8647590B2 (en) * 2006-03-16 2014-02-11 Agilent Technologies, Inc. Optical detection cell with micro-fluidic chip
US20070218454A1 (en) * 2006-03-16 2007-09-20 Brennen Reid A Optical detection cell for micro-fluidics
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US20080182136A1 (en) * 2007-01-26 2008-07-31 Arnold Don W Microscale Electrochemical Cell And Methods Incorporating The Cell
US7867592B2 (en) 2007-01-30 2011-01-11 Eksigent Technologies, Inc. Methods, compositions and devices, including electroosmotic pumps, comprising coated porous surfaces
ES2797951T3 (en) 2007-04-04 2020-12-04 Ande Corp Integrated nucleic acid analysis
WO2009076134A1 (en) 2007-12-11 2009-06-18 Eksigent Technologies, Llc Electrokinetic pump with fixed stroke volume
CN101738449B (en) * 2008-11-07 2011-08-31 中国科学院大连化学物理研究所 Electrode bracket and applications thereof in micro-fluidic chip system
US20100199750A1 (en) * 2009-02-06 2010-08-12 Arnold Don W Microfludic Analysis System and Method
JP2012529908A (en) 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド Improved method for quantification of forensic DNA
US8951471B2 (en) * 2010-02-26 2015-02-10 Perkinelmer Health Sciences, Inc. Jet assembly for use in detectors and other devices
CN101823686B (en) * 2010-04-21 2012-07-04 大连理工大学 Sealing method of thermoplastic polymer multi-layer micro-fluidic chips
GB201011019D0 (en) 2010-07-01 2010-08-18 Weston Richard Ionisation mass spectrometry
SG11201406551WA (en) * 2012-07-12 2014-11-27 Agency Science Tech & Res A connector for microfluidic device, a method for injecting fluid into microfluidic device using the connector and a method of providing and operating a valve
US9453996B2 (en) 2013-10-23 2016-09-27 Tokitae Llc Devices and methods for staining and microscopy
DE102015120860B4 (en) * 2014-12-02 2022-10-20 Micromass Uk Limited Annular counter-electrode for improving beam stability and junction sensitivity on a ceramic tile-type microfluidic device
US10258741B2 (en) 2016-12-28 2019-04-16 Cequr Sa Microfluidic flow restrictor and system
CN114870917B (en) * 2022-05-09 2023-06-06 南京大学 Microfluidic chip for identifying different cells, preparation method thereof and detection platform

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4052132A (en) * 1975-11-10 1977-10-04 Oates William L Method and apparatus for drilling fine holes in frangible workpieces
US4394263A (en) * 1980-12-04 1983-07-19 Carlo Erba Strumentazione S.P.A. Connecting device for pneumatic and/or hydraulic circuits in column chromatography
JPS6228664A (en) * 1985-07-31 1987-02-06 Sord Comput Corp Method for connecting silicon wafer capillary column with pipe
EP0400377A2 (en) * 1989-05-31 1990-12-05 The Whitaker Corporation Construction of an optical fiber with a connector
US5132012A (en) * 1988-06-24 1992-07-21 Hitachi, Ltd. Liquid chromatograph
WO1997004297A1 (en) * 1995-07-21 1997-02-06 Northeastern University Microscale fluid handling system
US5744100A (en) * 1993-03-12 1998-04-28 Orion Research, Inc. Connectorized capillaries for use with separation instrumentation components

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2156156A (en) * 1935-07-15 1939-04-25 Mahlck Gustave Method of producing grooves or channels in dielectric materials
US3518008A (en) * 1966-06-10 1970-06-30 Technicon Corp Flow cell device
US3538744A (en) * 1967-11-09 1970-11-10 Phillips Petroleum Co Chromatography apparatus
BE792114A (en) * 1971-12-17 1973-05-30 Technicon Instr CONTINUOUS FLOW CELL FOR LIGHT MEASUREMENT ON FLUID
DE2229723C2 (en) * 1972-06-19 1982-05-27 Hellma GmbH u.Co KG Glastechnische-optische Werkstätten, 7840 Müllheim Flow cell for optical measurements
US3831618A (en) * 1972-12-22 1974-08-27 Abbott Lab Apparatus for the precision metering of fluids
DE2413703C3 (en) * 1974-03-21 1979-01-04 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V., 3400 Goettingen Valve arrangement for the supply of liquid or gaseous substances to a processing vessel
DE2621895C2 (en) * 1976-05-17 1986-07-24 Hellma GmbH u.Co KG Glastechnische-optische Werkstätten, 7840 Müllheim Flow cell
US4351189A (en) * 1979-08-17 1982-09-28 National Research Development Corporation Differential flowmeters
DE3316751C2 (en) * 1983-05-07 1986-08-28 Hellma GmbH u.Co KG Glastechnische-optische Werkstätten, 7840 Müllheim Flow cell
US4908112A (en) * 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US4883760A (en) * 1988-06-20 1989-11-28 Adi Diagnostics Inc. Device for performing enzyme immunoassays
US5540464A (en) * 1994-10-04 1996-07-30 J&W Scientific Incorporated Capillary connector
US5779868A (en) * 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
WO1998010122A1 (en) * 1996-09-03 1998-03-12 Northeastern University Microfabricated hybrid capillary array and multichannel detection assembly
US5969353A (en) * 1998-01-22 1999-10-19 Millennium Pharmaceuticals, Inc. Microfluid chip mass spectrometer interface

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4052132A (en) * 1975-11-10 1977-10-04 Oates William L Method and apparatus for drilling fine holes in frangible workpieces
US4394263A (en) * 1980-12-04 1983-07-19 Carlo Erba Strumentazione S.P.A. Connecting device for pneumatic and/or hydraulic circuits in column chromatography
JPS6228664A (en) * 1985-07-31 1987-02-06 Sord Comput Corp Method for connecting silicon wafer capillary column with pipe
US5132012A (en) * 1988-06-24 1992-07-21 Hitachi, Ltd. Liquid chromatograph
EP0400377A2 (en) * 1989-05-31 1990-12-05 The Whitaker Corporation Construction of an optical fiber with a connector
US5744100A (en) * 1993-03-12 1998-04-28 Orion Research, Inc. Connectorized capillaries for use with separation instrumentation components
WO1997004297A1 (en) * 1995-07-21 1997-02-06 Northeastern University Microscale fluid handling system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 011, no. 207 (P - 592) 4 July 1987 (1987-07-04) *

Also Published As

Publication number Publication date
CA2386056A1 (en) 2000-04-20
DE69917476T2 (en) 2004-10-07
US20030211631A1 (en) 2003-11-13
US6605472B1 (en) 2003-08-12
EP1127258B1 (en) 2004-05-19
DE69917476D1 (en) 2004-06-24
WO2000022409A2 (en) 2000-04-20
ATE267393T1 (en) 2004-06-15
EP1127258A2 (en) 2001-08-29

Similar Documents

Publication Publication Date Title
WO2000022409A3 (en) Microfluidic devices connected to capillaries with minimal dead volume
WO2002030486A3 (en) Microfluidic device based sample injection system for analytical devices
SE0501600L (en) Cuvette, method and forming tool for making thereof
WO2002064253A3 (en) Method and apparatus for sample injection in microfabricated devices
WO2003104814A3 (en) Biomolecule open channel solid phase extraction systems and methods
WO2005019804A3 (en) Microfluidic chip with enhanced tip for stable electrospray ionization
EP1500937A4 (en) Analysis instrument, sample analysis method and analysis device using the instrument, and method of forming opening in the instrument
WO2005084530A3 (en) Body fluid sampling device
DK1005603T3 (en) Methods for sampling seabed with compression tube
WO2007069088A3 (en) Sampling device
WO2005016125A3 (en) Method and apparatus for body fluid sampling with integrated analyte detecting member
WO2001089691A3 (en) Capillaries for fluid movement within microfluidic channels
WO2004034057A3 (en) System and method for high throughput screening of droplets
WO2005115888A3 (en) Electrospray ion source apparatus
EP2711415A3 (en) Assay cartridges and methods of using the same
WO2009057273A1 (en) Analyzing device, analyzing apparatus using the device, and analyzing method
IL169884A0 (en) Assay device
WO2009073155A3 (en) Fluid sample collecting and analyzing apparatus
EP1046913A3 (en) Immunochromatographic assay
ATE494455T1 (en) DEVICE FOR PRESSING MOUNTAIN ANCHORS
WO2008087866A1 (en) Ionization emitter, ionization apparatus, and method for manufacturing ionization emitter
WO2006138629A3 (en) Method of classifying chemically crosslinked cellular samples using mass spectra
DE10207340A1 (en) Sealing technology for capillary columns
WO2005020925A3 (en) Method and apparatus for dosing solids
WO2005011832A3 (en) A simultaneous multi-colum liquid chromatograph for direct sampling of an array of liquid samples

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): CA US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): CA US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

WWE Wipo information: entry into national phase

Ref document number: 1999944204

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999944204

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2386056

Country of ref document: CA

WWG Wipo information: grant in national office

Ref document number: 1999944204

Country of ref document: EP