WO2000024941B1 - Detection of target analytes using particles and electrodes - Google Patents

Detection of target analytes using particles and electrodes

Info

Publication number
WO2000024941B1
WO2000024941B1 PCT/US1999/025464 US9925464W WO0024941B1 WO 2000024941 B1 WO2000024941 B1 WO 2000024941B1 US 9925464 W US9925464 W US 9925464W WO 0024941 B1 WO0024941 B1 WO 0024941B1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
particle
transport
target analyte
reporter
Prior art date
Application number
PCT/US1999/025464
Other languages
French (fr)
Other versions
WO2000024941A1 (en
Inventor
Cynthia Bamdad
Robert Mucic
Original Assignee
Clinical Micro Sensors Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clinical Micro Sensors Inc filed Critical Clinical Micro Sensors Inc
Priority to AU12410/00A priority Critical patent/AU1241000A/en
Publication of WO2000024941A1 publication Critical patent/WO2000024941A1/en
Publication of WO2000024941B1 publication Critical patent/WO2000024941B1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/773—Nanoparticle, i.e. structure having three dimensions of 100 nm or less
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/92—Detection of biochemical
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.

Claims

AMENDED CLAIMS[received by the International Bureau on 1 1 May 2000 (1 1.05.00); new claims 7-17 added; remaining claims unchanged (2 pages)]
1. A composition comprising a colloid particle comprising at least one electron transfer moiety (ETM).
2. A composition according to claim 1 wherein said particle further comprising a self-assembled monolayer (SAM).
3. A composition according to claim 1 wherein said colloid further comprises at least one covalently attached nucleic acid.
4. A composition comprising a colloid particle comprising a covalently attached conductive oligomer.
5. A composition according to claim 4 wherein said colloid particle further comprises at one ETM.
6. A composition comprising a colloid particle comprising: a) a SAM; b) a capture probe; c) an amplification sequence; and d) a label probe hybridized to said amplification sequence, wherein said label probe comprises at least one covalently attached ETM.
7. A method of detecting a target analyte in a sample comprising: a) adding said sample to a composition comprising: i) a transport composition comprising a first binding partner that directly or indirectly binds a target analyte; ii) a reporter composition comprising:
1) a second binding partner that directly or indirectly binds said target analyte; and
2) a plurality of electron transfer moieties (ETMs); and iii) an electrode; wherein at least one of said transport and said reporter compositions is a particle; such that said target analyte binds to said transport composition and said reporter composition to form an assay complex; b) transporting said assay complex to said electrode; and c) detecting the presence of said ETMs.
8. A method according to claim 7 wherein said electrode comprises a self-assembled monolayer (SAM) comprising conductive oligomers.
85
9. A method according to claim 8 wherein said SAM further comprises insulators.
10. A method according to claim 7 wherein said transport composition is a transport particle.
11. A method according to claim 9 wherein said transport composition is a magnetic particle.
12. A method according to claim 9 wherein said transport composition is a colloid particle.
13. A method according to claim 7 wherein said reporter composition comprises a reporter particle.
14. A method according to claim 13 wherein said reporter particle is a colloid particle.
15. A method according to claim 7 wherein said target analyte is a nucleic acid and said first and second binding partners are nucleic acids.
16. A method according to claim 7 wherein said target analyte is a protein and said first and said second binding partners are proteins.
17. A method of detecting the presence of an ETM on the surface of a colloid comprising providing an input AC signal and detecting an output signal.
86
PCT/US1999/025464 1998-10-27 1999-10-27 Detection of target analytes using particles and electrodes WO2000024941A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU12410/00A AU1241000A (en) 1998-10-27 1999-10-27 Detection of target analytes using particles and electrodes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10587598P 1998-10-27 1998-10-27
US60/105,875 1998-10-27

Publications (2)

Publication Number Publication Date
WO2000024941A1 WO2000024941A1 (en) 2000-05-04
WO2000024941B1 true WO2000024941B1 (en) 2000-06-29

Family

ID=22308256

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/025464 WO2000024941A1 (en) 1998-10-27 1999-10-27 Detection of target analytes using particles and electrodes

Country Status (3)

Country Link
US (7) US6541617B1 (en)
AU (1) AU1241000A (en)
WO (1) WO2000024941A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895255B1 (en) 2003-07-12 2014-11-25 Accelerate Diagnostics, Inc. Sensitive and rapid determination of antimicrobial susceptibility
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems

Families Citing this family (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2097766T3 (en) 1989-08-16 1997-04-16 Chiron Corp COMPOSITIONS FOR THE INHIBITION OF THE FORMATION OF PROTEIN HORMONES AND THEIR USES.
US6013459A (en) * 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
DK0988534T3 (en) 1997-06-12 2011-05-23 Clinical Micro Sensors Inc Electronic methods and apparatus for detecting analytes
US7229841B2 (en) * 2001-04-30 2007-06-12 Cytimmune Sciences, Inc. Colloidal metal compositions and methods
US6761816B1 (en) * 1998-06-23 2004-07-13 Clinical Micro Systems, Inc. Printed circuit boards with monolayers and capture ligands
AU1241000A (en) * 1998-10-27 2000-05-15 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
US6833267B1 (en) * 1998-12-30 2004-12-21 Clinical Micro Sensors, Inc. Tissue collection devices containing biosensors
US20050148101A1 (en) * 1999-01-23 2005-07-07 Bamdad Cynthia C. Interaction of colloid-immobilized species with species on non-colloidal structures
WO2002001230A2 (en) * 2000-06-23 2002-01-03 Minerva Biotechnologies Corporation Rapid and sensitive detection of protein aggregation
US6942771B1 (en) * 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US20040053290A1 (en) * 2000-01-11 2004-03-18 Terbrueggen Robert Henry Devices and methods for biochip multiplexing
US7312087B2 (en) * 2000-01-11 2007-12-25 Clinical Micro Sensors, Inc. Devices and methods for biochip multiplexing
CA2380258C (en) * 1999-07-26 2008-07-15 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
US6361958B1 (en) * 1999-11-12 2002-03-26 Motorola, Inc. Biochannel assay for hybridization with biomaterial
GB9928232D0 (en) * 1999-12-01 2000-01-26 Skelton Stephen Detection system
US7829313B2 (en) 2000-03-24 2010-11-09 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
US20080085515A1 (en) * 2000-03-24 2008-04-10 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays
US7875442B2 (en) * 2000-03-24 2011-01-25 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
US7205104B2 (en) * 2000-03-24 2007-04-17 Eppendorf Array Technologies Sa (Eat) Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays
US7202026B2 (en) * 2000-03-24 2007-04-10 Eppendorf Array Technologies Sa (Eat) Identification of a large number of biological (micro)organisms groups at different levels by their detection on a same array
EP1164201A1 (en) * 2000-06-14 2001-12-19 Facultés Universitaires Notre-Dame de la Paix Reverse detection for identification and/or quantification of nucleotide target sequences on biochips
JP4272800B2 (en) * 2000-06-22 2009-06-03 富士フイルム株式会社 Gene expression analysis using multiple potentials
AU2001270078A1 (en) * 2000-06-23 2002-01-08 Minerva Biotechnologies Corporation Tandem signaling assay
US6706474B1 (en) * 2000-06-27 2004-03-16 Board Of Trustees Of The University Of Illinois Nucleic acid enzyme biosensors for ions
US7615340B2 (en) * 2000-10-03 2009-11-10 Minerva Biotechnologies Corporation Electronic detection of interaction and detection of interaction based on the interruption of flow
US20080202927A1 (en) * 2000-11-03 2008-08-28 Clinical Micro Sensors, Inc. Devices and methods for biochip multiplexing
EP1348034B1 (en) * 2000-11-15 2016-07-20 Minerva Biotechnologies Corporation Oligonucleotide identifiers
WO2003062783A2 (en) * 2001-07-20 2003-07-31 North Carolina State University Light addressable electrochemical detection of duplex structures
US20050003360A1 (en) * 2001-11-13 2005-01-06 Ruo-Pang Huang Array systems and methods
US20030186301A1 (en) * 2002-03-25 2003-10-02 The Regents Of The University Of California Constructing user-defined, long DNA sequences
US6890719B2 (en) * 2002-05-10 2005-05-10 The Board Of Trustess Of The University Of Illinois Fluorescence based biosensor
US7534560B2 (en) * 2002-05-10 2009-05-19 The Board Of Trustees Of The University Of Illinois Simple catalytic DNA biosensors for ions based on color changes
US7687258B1 (en) * 2002-05-20 2010-03-30 Maki Wusi C Direct electric biological agent detector
US7176036B2 (en) * 2002-09-20 2007-02-13 Arrowhead Center, Inc. Electroactive microspheres and methods
WO2004059347A2 (en) * 2002-12-20 2004-07-15 Minerva Biotechnologies Corporation Optical devices and methods involving nanoparticles
US20040180369A1 (en) * 2003-01-16 2004-09-16 North Carolina State University Photothermal detection of nucleic acid hybridization
US7612185B2 (en) * 2003-03-07 2009-11-03 The Board Of Trustees Of The University Of Illinois Nucleic acid biosensors
WO2004098386A2 (en) * 2003-05-01 2004-11-18 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch
US20120077206A1 (en) 2003-07-12 2012-03-29 Accelr8 Technology Corporation Rapid Microbial Detection and Antimicrobial Susceptibility Testing
US7341841B2 (en) 2003-07-12 2008-03-11 Accelr8 Technology Corporation Rapid microbial detection and antimicrobial susceptibility testing
WO2005042785A1 (en) * 2003-10-30 2005-05-12 North Carolina State University Electrochemical detection of nucleic acid hybridization
US7485419B2 (en) * 2004-01-13 2009-02-03 The Board Of Trustees Of The University Of Illinois Biosensors based on directed assembly of particles
US7338763B2 (en) * 2004-06-02 2008-03-04 Eppendorf Array Technologies S.A. Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays
US20050287536A1 (en) * 2004-06-24 2005-12-29 Douglas Kozlay Disposable, multi-use, DNA sample collection disk apparatus for field biohazard testing, DNA testing, and personal authentication
US7309875B2 (en) * 2004-11-22 2007-12-18 Hewlett-Packard Development Company, L.P. Nanocrystal protective layer for crossbar molecular electronic devices
WO2007044029A2 (en) * 2004-12-03 2007-04-19 Nano Science Diagnostic, Inc. Method and apparatus for low quantity detection of bioparticles in small sample volumes
US20060166222A1 (en) * 2005-01-21 2006-07-27 Yi Lu Nucleic acid enzyme ligation sensor
CA2599013A1 (en) * 2005-02-28 2006-09-08 Gen-Probe Incorporated Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe
EP1909097B1 (en) * 2005-06-06 2012-04-18 Nikkiso Company Limited Biosensor and biosensor cell
CA2656004A1 (en) * 2005-06-17 2006-12-28 University Of Maryland Biotechnology Institute Metal-enhanced fluorescence-based sensing methods
WO2007016467A2 (en) * 2005-08-02 2007-02-08 Michigan State University Nanoporous silicon-based electrochemical dna biosensor
US7892734B2 (en) * 2005-08-11 2011-02-22 The Board Of Trustees Of The University Of Illinois Aptamer based colorimetric sensor systems
US20090036315A1 (en) * 2006-02-07 2009-02-05 Antara Biosciences Inc. Device and methods for detecting and quantifying one or more target agents
US7799554B2 (en) * 2006-03-16 2010-09-21 The Board Of Trustees Of The University Of Illinois Lateral flow devices
WO2008069479A1 (en) * 2006-12-06 2008-06-12 Electronics And Telecommunications Research Institute Substrate for analzing coverage of self-assembled molecules and analyzing method using the same
KR100900955B1 (en) 2006-12-06 2009-06-08 한국전자통신연구원 Substrates for analyzing the coverage of self-assembled molecules and methods of analyzing the coverage of self-assembled molecules using the same
WO2008089248A2 (en) * 2007-01-19 2008-07-24 The Board Of Trustees Of The University Of Illinois Amphiphilic substances and triggered liberation from lipid vesicles
US7863035B2 (en) 2007-02-15 2011-01-04 Osmetech Technology Inc. Fluidics devices
US8058415B2 (en) 2007-04-24 2011-11-15 The Board Of Trustees Of The University Of Illinois Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes
US8409800B2 (en) * 2007-07-16 2013-04-02 The Board Of Trustees Of The University Of Illinois Nucleic acid based fluorescent sensor for copper detection
US8568690B2 (en) * 2007-07-31 2013-10-29 The Board Of Trustees Of The University Of Illinois MRI contrast agents and high-throughput screening by MRI
WO2009045632A2 (en) 2007-08-10 2009-04-09 The Board Of Trustees Of The University Of Illinois Nucleic acid based fluorescent sensor for mercury detection
EP2200932A4 (en) * 2007-09-21 2014-09-10 Cytimmune Sciences Inc Nanotherapeutic colloidal metal compositions and methods
US20110014118A1 (en) * 2007-09-21 2011-01-20 Lawrence Tamarkin Nanotherapeutic colloidal metal compositions and methods
WO2009052436A1 (en) * 2007-10-17 2009-04-23 Ohmx Corporation Novel chemistry used in biosensors
US8200310B2 (en) * 2007-11-19 2012-06-12 University Of Southern California Spinal injury imaging by magnetically levitated sensors
WO2009066293A1 (en) * 2007-11-20 2009-05-28 Technion Research And Development Foundation Ltd. Chemical sensors based on cubic nanoparticles capped with an organic coating
IL190475A0 (en) * 2008-03-27 2009-02-11 Technion Res & Dev Foundation Chemical sensors based on cubic nanoparticles capped with organic coating for detecting explosives
EP2278333B1 (en) * 2008-04-10 2013-06-12 Probiond Co., Ltd. New signal amplification technique for mass analysis
US20100105039A1 (en) * 2008-06-03 2010-04-29 Yi Lu Label-free colorimetric detection
JP5430093B2 (en) * 2008-07-24 2014-02-26 デクセリアルズ株式会社 Conductive particles, anisotropic conductive film, joined body, and connection method
US8062893B2 (en) 2008-10-10 2011-11-22 The Board Of Trustees Of The University Of Illinois Fluorescent sensor for mercury
DK2189539T4 (en) * 2008-11-21 2018-09-17 Chimera Biotec Gmbh Conjugate complexes for analyte detection
JP5352742B2 (en) * 2009-08-07 2013-11-27 オームクス コーポレーション Enzymatic redox-change chemical elimination (E-TRACE) immunoassay
US9250234B2 (en) 2011-01-19 2016-02-02 Ohmx Corporation Enzyme triggered redox altering chemical elimination (E-TRACE) immunoassay
CA2798812A1 (en) 2010-05-21 2011-11-24 Ohmx Corporation Detection of cancer by assaying psa enzymatic activity
US8815156B2 (en) 2010-07-19 2014-08-26 Andalyze, Inc. Sensor housing and reagent chemistry
WO2012012537A1 (en) 2010-07-20 2012-01-26 Ohmx Corporation Novel chemistry used in biosensors
US10254204B2 (en) 2011-03-07 2019-04-09 Accelerate Diagnostics, Inc. Membrane-assisted purification
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
EP2768967B1 (en) 2011-10-17 2017-12-06 Ohmx Corporation Single, direct detection of hemoglobin a1c percentage using enzyme triggered redox altering chemical elimination (e-trace) immunoassay
US9340567B2 (en) 2011-11-04 2016-05-17 Ohmx Corporation Chemistry used in biosensors
JP6110871B2 (en) * 2011-12-02 2017-04-05 コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． Method and apparatus for detecting the pH value of a solution
GB201121241D0 (en) * 2011-12-12 2012-01-18 Isis Innovation Labelled nanoparticles and methods for their modification and detection
GB2497972B (en) * 2011-12-23 2016-03-16 Schlumberger Holdings Electrochemical sensors
JP6276706B2 (en) 2012-01-09 2018-02-07 オームクス コーポレイション Enzyme cascade method for E-TRACE assay signal amplification
JP2015529809A (en) 2012-07-27 2015-10-08 オームクス コーポレイション Electrical measurement of monolayers after pre-cleavage detection of the presence and activity of enzymes and other target analytes
US9404883B2 (en) 2012-07-27 2016-08-02 Ohmx Corporation Electronic measurements of monolayers following homogeneous reactions of their components
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
EP2912432B1 (en) 2012-10-24 2018-07-04 Genmark Diagnostics Inc. Integrated multiplex target analysis
WO2014124322A1 (en) * 2013-02-08 2014-08-14 University Of Louisville Research Foundation, Inc. Nanoparticles for drug delivery
EP3520895A1 (en) 2013-03-15 2019-08-07 Genmark Diagnostics Inc. Fluid container with cantilevered lance
US9677109B2 (en) 2013-03-15 2017-06-13 Accelerate Diagnostics, Inc. Rapid determination of microbial growth and antimicrobial susceptibility
EP3047274A2 (en) 2013-09-20 2016-07-27 Ohmx Corporation Psa enzymatic activity: a new biomarker for assessing prostate cancer aggressiveness
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
EP3080294B1 (en) 2013-12-12 2018-06-13 Altratech Limited A capacitive sensor and method of use
EP2883961B8 (en) * 2013-12-12 2017-09-27 Altratech Limited A nucleic acid analysis method and apparatus
EP3080267B1 (en) 2013-12-12 2020-02-19 Altratech Limited A sample preparation method and apparatus
US10436772B2 (en) 2014-08-25 2019-10-08 United Arab Emirates University Method and system for counting white blood cells electrically
US10564123B2 (en) 2014-05-25 2020-02-18 United Arab Emirates University Bioreactor system and method of operating same for cellular composition identification and quantification
US20160041118A1 (en) 2014-08-11 2016-02-11 Ohmx Corporation Enzyme triggered redox altering chemical elimination (e-trace) assay with multiplexing capabilities
US10078067B2 (en) * 2014-08-25 2018-09-18 United Arab Emirates University Apparatus and method for detection and quantification of biological and chemical analytes
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
EP3218108B1 (en) 2014-11-11 2020-09-02 Genmark Diagnostics Inc. Fluid sample processing cartridge and use thereof
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
US20160178562A1 (en) 2014-12-19 2016-06-23 Ohmx Corporation Competitive enzymatic assay
KR20170132856A (en) 2015-03-30 2017-12-04 액셀러레이트 다이어그노스틱스, 아이엔씨. Instruments and systems for rapid microbiological identification and antimicrobial susceptibility testing
US10253355B2 (en) 2015-03-30 2019-04-09 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
WO2016160877A1 (en) * 2015-03-31 2016-10-06 Rapid Pathogen Screening, Inc. Non-enzymatic nucleic acid detection using an oligonucleotide linker with a large cell gap
US10753940B2 (en) 2015-08-03 2020-08-25 President And Fellows Of Harvard College Enhanced electrochemical detection using nanoparticles and precipitation
CN106053429B (en) * 2016-05-27 2019-04-05 福建师范大学 Urine modified nucleoside determination method based on surface enhanced resonance raman spectra
GB2552461B (en) * 2016-07-14 2022-06-15 Rightangled Ltd Method and assay kit to detect an analyte
CN107783159B (en) * 2016-08-31 2023-07-25 松下电器（美国）知识产权公司 Position measurement system, position measurement method, and mobile robot
EP3516401A1 (en) 2016-09-19 2019-07-31 Genmark Diagnostics Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
EP3574309A4 (en) * 2017-01-26 2020-11-11 Vital Biosciences, Inc. Magnetic particle-based immunoassay and methods of using the same
WO2018165566A1 (en) 2017-03-09 2018-09-13 Auburn University Differential circuit for background correction in electrochemical measurements
WO2019005473A1 (en) * 2017-06-28 2019-01-03 Accure Health Inc. Integrated opto nano electronic (ione) sensing
WO2019010341A1 (en) 2017-07-07 2019-01-10 Innamed, Inc. Aptamers for measuring lipoprotein levels
US11331019B2 (en) 2017-08-07 2022-05-17 The Research Foundation For The State University Of New York Nanoparticle sensor having a nanofibrous membrane scaffold
WO2019040769A1 (en) 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
US20190062809A1 (en) 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
US11459601B2 (en) 2017-09-20 2022-10-04 Altratech Limited Diagnostic device and system
JP7077013B2 (en) 2017-12-27 2022-05-30 株式会社トプコン 3D information processing unit, device equipped with 3D information processing unit, unmanned aerial vehicle, notification device, moving object control method using 3D information processing unit, and program for moving object control processing
US10935561B2 (en) 2018-06-01 2021-03-02 Genmark Diagnostics, Inc. Integrated diagnostic instrument
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
US10948450B2 (en) 2018-07-23 2021-03-16 Genmark Diagnostics, Inc. Electrochemical measurements of components in coatings
EP3842548A4 (en) * 2018-08-14 2022-04-27 Korea University Research and Business Foundation Method for synthesizing single metal nanobridged structure and method for manufacturing dna point mutation detection sensor by using same
US11408850B2 (en) * 2020-06-22 2022-08-09 The Florida International University Board Of Trustees Aptamer-based sensors for detection of fentanyl opioids

Family Cites Families (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US694771A (en) * 1901-06-08 1902-03-04 Christopher Whitehead Telescopic fire-escape and convertible scaffolding.
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1219824A (en) 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
US5241060A (en) 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
GB8328520D0 (en) 1983-10-25 1983-11-23 Serono Diagnostics Ltd Methods of assay
WO1985002627A1 (en) 1983-12-16 1985-06-20 Genetics International, Inc. Assay for nucleic acids
US4849513A (en) 1983-12-20 1989-07-18 California Institute Of Technology Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
US4707440A (en) 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US4707352A (en) 1984-01-30 1987-11-17 Enzo Biochem, Inc. Method of radioactively labeling diagnostic and therapeutic agents containing a chelating group
US5175269A (en) 1984-01-30 1992-12-29 Enzo Diagnostics, Inc. Compound and detectable molecules having an oligo- or polynucleotide with modifiable reactive group
US4952685A (en) 1984-01-30 1990-08-28 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US5013831A (en) 1984-01-30 1991-05-07 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US4943523A (en) 1984-01-30 1990-07-24 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US5002885A (en) 1984-01-30 1991-03-26 Enzo Biochem, Inc. Detectable molecules, method preparation and use
CA1260372A (en) 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
GB8417301D0 (en) 1984-07-06 1984-08-08 Serono Diagnostics Ltd Assay
US4755458A (en) 1984-08-30 1988-07-05 Enzo Biochem, Inc. Composition and method for the detection of the presence of a polynucleotide sequence of interest
US5112974A (en) 1985-01-18 1992-05-12 The Trustees Of Columbia University In The City Of New York Mixed ligand complexes and uses thereof as binding agents to DNA
GB8507706D0 (en) 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US5595908A (en) 1985-09-26 1997-01-21 University Of Southern Mississipi Piezoelectric device for detection of polynucleotide hybridization
CA1273552A (en) 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
CA1293579C (en) 1986-01-17 1991-12-24 Akio Kashihara Crosslinked polymer microparticles and coating composition containing the same
US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
CA1254945A (en) 1986-02-27 1989-05-30 Marco F. Cardosi Application of tetrathiafulvalenes in bioelectrochemical processes
JPS63238166A (en) 1987-03-26 1988-10-04 Mitsubishi Electric Corp Organic electronic element material
US5082830A (en) 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
GB2217447A (en) 1988-04-21 1989-10-25 Atomic Energy Authority Uk Bonding to metal surfaces
JP2529590B2 (en) 1988-07-29 1996-08-28 株式会社アルファ Infrared receiver for vehicle
SE462454B (en) * 1988-11-10 1990-06-25 Pharmacia Ab METHOD FOR USE IN BIOSENSORS
US5089112A (en) 1989-03-20 1992-02-18 Associated Universities, Inc. Electrochemical biosensor based on immobilized enzymes and redox polymers
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
CA2033692A1 (en) 1990-01-25 1991-07-26 Wilhelm Bannwarth Energy transfer systems
US5108573A (en) * 1990-06-05 1992-04-28 The United States Of America As Represented By The United States Department Of Energy Morphology in electrochemically grown conducting polymer films
US5776672A (en) 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
US5294369A (en) * 1990-12-05 1994-03-15 Akzo N.V. Ligand gold bonding
DE4039677A1 (en) 1990-12-12 1992-06-17 Boehringer Mannheim Gmbh UNIVERSAL BINDING FILM
US5180968A (en) 1991-03-01 1993-01-19 Research Foundation Of State University Of New York Method and apparatus for compensation of double layer charging current in electrochemical cells
JPH04278450A (en) 1991-03-04 1992-10-05 Adam Heller Biosensor and method for analyzing subject
RU1794088C (en) 1991-03-18 1993-02-07 Институт Молекулярной Биологии Ан@ Ссср Method of dna nucleotide sequence determination and a device for its realization
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US6652808B1 (en) * 1991-11-07 2003-11-25 Nanotronics, Inc. Methods for the electronic assembly and fabrication of devices
AU658962B2 (en) 1991-11-15 1995-05-04 Igen, Inc. Rapid assays for amplification products
IL103674A0 (en) * 1991-11-19 1993-04-04 Houston Advanced Res Center Method and apparatus for molecule detection
US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5565552A (en) 1992-01-21 1996-10-15 Pharmacyclics, Inc. Method of expanded porphyrin-oligonucleotide conjugate synthesis
WO1993016383A1 (en) 1992-02-08 1993-08-19 Genera Technologies Limited Methods of analysis
US5391272A (en) * 1992-03-06 1995-02-21 Andcare, Inc. Electrochemical immunoassay methods
DE69207580T2 (en) 1992-03-23 1996-08-08 Hoffmann La Roche Procedure for DNA detection
CA2094785A1 (en) 1992-05-08 1993-11-09 Jean Gariepy Metal chelating peptide
US5565658A (en) * 1992-07-13 1996-10-15 Cirque Corporation Capacitance-based proximity with interference rejection apparatus and methods
JPH0641183A (en) 1992-07-23 1994-02-15 Mitsubishi Kasei Corp Monomolecular film of oligonucleotide
IL102930A (en) * 1992-08-25 1997-03-18 Yissum Res Dev Co Electrobiochemical analytical method and electrodes
US5312527A (en) 1992-10-06 1994-05-17 Concordia University Voltammetric sequence-selective sensor for target polynucleotide sequences
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
EP0599337B1 (en) 1992-11-27 2006-03-08 Canon Kabushiki Kaisha Method for detection of nucleic acid and probe therefor
US5403451A (en) 1993-03-05 1995-04-04 Riviello; John M. Method and apparatus for pulsed electrochemical detection using polymer electroactive electrodes
FR2703359B1 (en) 1993-03-31 1995-06-23 Cis Bio Int Nucleotide (s) / electronic conductive polymer; its preparation process and its use.
RU2041262C1 (en) 1993-08-11 1995-08-09 Институт молекулярной биологии им.В.А.Энгельгардта РАН Method for immobilization of water soluble bioorganic compounds on capillary-porous carrier
RU2041263C1 (en) 1993-08-11 1995-08-09 Геннадий Моисеевич Ершов Method and apparatus for microdosing and dispensing of aqueous solutions onto carrier
RU2041261C1 (en) 1993-08-11 1995-08-09 Институт молекулярной биологии им.В.А.Энгельгардта РАН Method for manufacturing of matrix for detecting of mismatches
US5965452A (en) 1996-07-09 1999-10-12 Nanogen, Inc. Multiplexed active biologic array
US5824473A (en) * 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5952172A (en) * 1993-12-10 1999-09-14 California Institute Of Technology Nucleic acid mediated electron transfer
US5591578A (en) 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US6071699A (en) * 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
IL108726A (en) 1994-02-22 1999-12-31 Yissum Res Dev Co Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium and electrodes therefor
US5620850A (en) 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
US5601982A (en) 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US6207369B1 (en) 1995-03-10 2001-03-27 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
US6319670B1 (en) * 1995-05-09 2001-11-20 Meso Scale Technology Llp Methods and apparatus for improved luminescence assays using microparticles
CN1192249A (en) 1995-06-27 1998-09-02 查珀尔希尔北卡罗来纳大学 Electrochemical detection of nucleic acid hybridization
US5830341A (en) 1996-01-23 1998-11-03 Gilmartin; Markas A. T. Electrodes and metallo isoindole ringed compounds
US5861247A (en) 1996-01-26 1999-01-19 University Of Chicago Rapid method to detect duplex formation in sequencing by hybridization methods
US5851772A (en) 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
US6107080A (en) 1996-04-25 2000-08-22 Mcgill University Biosensor device and method
DE69719817T2 (en) 1996-04-25 2003-12-24 Pence Inc BIOSENSOR DEVICE AND METHOD
JP2000513811A (en) 1996-05-22 2000-10-17 オーストラリアン メンブレイン アンド バイオテクノロジィ リサーチ インスティチュート Nucleic acid sensor
CA2257534A1 (en) 1996-06-07 1997-12-11 California Institute Of Technology Nucleic acid mediated electron transfer
AU4043497A (en) 1996-07-29 1998-02-20 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US5823447A (en) 1996-08-27 1998-10-20 Meritech, Inc. Angled fan nozzle and unibody cylinder
US5958791A (en) * 1996-09-27 1999-09-28 Innovative Biotechnologies, Inc. Interdigitated electrode arrays for liposome-enhanced immunoassay and test device
US7014992B1 (en) * 1996-11-05 2006-03-21 Clinical Micro Sensors, Inc. Conductive oligomers attached to electrodes and nucleoside analogs
CA2270633A1 (en) 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US7045285B1 (en) * 1996-11-05 2006-05-16 Clinical Micro Sensors, Inc. Electronic transfer moieties attached to peptide nucleic acids
US6096273A (en) * 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US7381525B1 (en) * 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
US5905024A (en) 1996-12-17 1999-05-18 University Of Chicago Method for performing site-specific affinity fractionation for use in DNA sequencing
WO1998028444A2 (en) 1996-12-23 1998-07-02 The University Of Chicago Customized oligonucleotide microchips as multiple biosensors
US6306584B1 (en) 1997-01-21 2001-10-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
KR20000070821A (en) 1997-02-06 2000-11-25 프란시스 제이 메이어 Electrochemical probes for detection of molecular interactions and drug discovery
US6391558B1 (en) * 1997-03-18 2002-05-21 Andcare, Inc. Electrochemical detection of nucleic acid sequences
US6060327A (en) 1997-05-14 2000-05-09 Keensense, Inc. Molecular wire injection sensors
DK0988534T3 (en) 1997-06-12 2011-05-23 Clinical Micro Sensors Inc Electronic methods and apparatus for detecting analytes
US6013459A (en) * 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
SE9703314D0 (en) * 1997-09-15 1997-09-15 Sangtec Medical Ab Capacity affinity sensor
US6008002A (en) * 1997-09-29 1999-12-28 Bodey; Bela Immunomagnetic detection and isolation of cancer cells
US6002817A (en) * 1997-09-29 1999-12-14 The Regents Of The University Of Michigan Optical sensors for the detection of nitric oxide
US20050053962A1 (en) * 1998-01-27 2005-03-10 Gary Blackburn Amplification of nucleic acids with electronic detection
US7090804B2 (en) * 1998-01-27 2006-08-15 Clinical Mirco Sensors, Inc. Amplification of nucleic acids with electronic detection
US6686150B1 (en) * 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US6063573A (en) * 1998-01-27 2000-05-16 Clinical Micro Sensors, Inc. Cycling probe technology using electron transfer detection
US6060023A (en) 1998-03-31 2000-05-09 Motorola, Inc. Molecular sensing apparatus
CA2327525A1 (en) 1998-05-06 1999-11-11 Cynthia Bamdad Electronic detection of nucleic acids using monolayers
AU763494B2 (en) * 1998-05-06 2003-07-24 Clinical Micro Sensors, Inc. Electronic methods for the detection of analytes utilizing monolayers
US20050244954A1 (en) * 1998-06-23 2005-11-03 Blackburn Gary F Binding acceleration techniques for the detection of analytes
US6761816B1 (en) * 1998-06-23 2004-07-13 Clinical Micro Systems, Inc. Printed circuit boards with monolayers and capture ligands
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6740518B1 (en) * 1998-09-17 2004-05-25 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes
AU768546B2 (en) 1998-09-17 2003-12-18 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes
AU1241000A (en) * 1998-10-27 2000-05-15 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
EP2000803A1 (en) 1998-11-30 2008-12-10 Nanosphere, Inc. Nanoparticles with Polymer Shells
CA2355875A1 (en) 1998-12-30 2000-07-06 Clinical Micro Sensors, Inc. Tissue collection devices containing biosensors
US6833267B1 (en) * 1998-12-30 2004-12-21 Clinical Micro Sensors, Inc. Tissue collection devices containing biosensors
US20020177135A1 (en) * 1999-07-27 2002-11-28 Doung Hau H. Devices and methods for biochip multiplexing
US20040053290A1 (en) * 2000-01-11 2004-03-18 Terbrueggen Robert Henry Devices and methods for biochip multiplexing
US6942771B1 (en) * 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US6258266B1 (en) * 1999-04-21 2001-07-10 Sta-Rite Industries, Inc. Faucet mount water filtration device
JP2003503699A (en) 1999-06-25 2003-01-28 ナノスフェアー インコーポレイテッド Oligonucleotide-Attached Nanoparticles and Methods of Use
WO2001006016A2 (en) 1999-07-20 2001-01-25 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
CA2380258C (en) 1999-07-26 2008-07-15 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
US6579726B1 (en) * 1999-07-30 2003-06-17 Surromed, Inc. Instruments, methods and reagents for surface plasmon resonance
WO2001035100A2 (en) 1999-11-12 2001-05-17 Clinical Micro Sensors, Inc. Binding acceleration techniques for the detection of analytes
ES2278757T3 (en) 2000-01-11 2007-08-16 Clinical Micro Sensors, Inc. CARTRIDGE UNDERSTANDING A BIOCHIP.
JP2004515231A (en) 2000-11-03 2004-05-27 クリニカル・マイクロ・センサーズ・インコーポレイテッド Apparatus and method for multiplexing biochips
WO2003085082A2 (en) 2001-04-03 2003-10-16 Clinical Micro Sensors, Inc. Nucleic acid reactions using labels with different redox potentials

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895255B1 (en) 2003-07-12 2014-11-25 Accelerate Diagnostics, Inc. Sensitive and rapid determination of antimicrobial susceptibility
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems

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