WO2000036139A1 - Hygiene monitoring - Google Patents

Hygiene monitoring Download PDF

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Publication number
WO2000036139A1
WO2000036139A1 PCT/GB1999/004244 GB9904244W WO0036139A1 WO 2000036139 A1 WO2000036139 A1 WO 2000036139A1 GB 9904244 W GB9904244 W GB 9904244W WO 0036139 A1 WO0036139 A1 WO 0036139A1
Authority
WO
WIPO (PCT)
Prior art keywords
glucose
sample
hygiene monitoring
atp
reagents
Prior art date
Application number
PCT/GB1999/004244
Other languages
French (fr)
Inventor
Peter Leonard Grant
Ramin Pirzad
Original Assignee
Celsis International Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celsis International Plc filed Critical Celsis International Plc
Priority to JP2000588387A priority Critical patent/JP2002532698A/en
Priority to EP99961211A priority patent/EP1141380A1/en
Priority to AU17897/00A priority patent/AU1789700A/en
Publication of WO2000036139A1 publication Critical patent/WO2000036139A1/en
Priority to US09/882,626 priority patent/US20020037590A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Definitions

  • This invention relates to hygiene monitoring, and in particular to an assay for the presence of food residues and/or microorganisms.
  • Hygiene monitoring procedures typically involve swabbing a surface, and analysing the sample taken up in the swab, for the presence of food residues and/or microorganisms.
  • a typical hygiene monitoring device is disclosed in WO-A-98/27196.
  • the ability to detect the presence of food residues and/or microorganisms in the sample that has been taken up by swabbing relies on the presence of ADP/ATP, and the ability to amplify the amount of ADP/ATP, and convert it to a detectable signal, also as disclosed in WO-A-94/25619.
  • the preferred amplification reaction involves, inter alia, the amplification of ATP and then the conversion of glucose-6-phosphate to glucose, and the conversion of glucose, via a sequence of enzymatic reactions, to a coloured end point.
  • glucose itself may be a sufficient indicator, for the purposes of an assay used in hygiene monitoring.
  • such an assay comprises the collection of a sample from a locus, and the determination of the presence of glucose in the sample.
  • the reagents disclosed in WO-A-94/25619 include those sufficient to convert glucose to a detectable signal; if desired, the reagents used in this invention may exclude those that convert ATP, but the signal generated in the presence of glucose appears relatively rapidly, and can thus be distinguished, in addition to the advantage of providing a rapid response. The amplification of ATP needed to generate the signal is relatively slow.
  • the locus to be tested is also assayed for the substantial absence of materials that may interfere in the novel assay, e.g. by inhibiting or by giving false positives, such as peroxide or reducing agents that may be incorporated in materials used to sanitise the locus.
  • the present invention can be practised utilising the same reagents as are disclosed in WO-A-94/25619, especially in so far as that relates to the conversion of glucose to a detectable signal. If desired, the reagents may exclude one or more of the components that are disclosed there for the conversion of ATP to glucose.
  • a suitable device that can be used for the purposes of swabbing and detection is disclosed in WO-A-98/27196 and WO-A-99/31218.
  • Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
  • Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
  • Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
  • Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
  • Swabs were taken from a variety of locations, and tested. In addition to determining the total viable count (TVC) and Enterobacteria (Enteros), contact plates were used alongside two devices.
  • systemSURE (“sSURE”) is a portable hygiene monitoring system (available from Becton Dickinson) that uses bioluminescence as an endpoint.
  • the "Pen Swab” is generally as disclosed in WO-A-99/31218. Results are shown in Table 1.
  • the colour reagents were used to determine the level at which glucose was detectable.
  • the reagents were activated in the presence of different concentrations of glucose.
  • the absorbance values for a complete colour change were obtained within 120 seconds. Results are shown in Table 2.
  • Swabs were taken from a variety of locations in a store, at different times of day and tested. Results are shown, for two different days, in Tables 3A and 3B. A large proportion of the data shows a rapid colour change (in seconds), characteristic of glucose detection. The corresponding RLU values do not show the presence of gross amounts of ATP, which is consistent with the detection of glucose. Table 3A

Abstract

A hygiene monitoring method comprises the collection of a sample from a locus, and the determination of the presence of glucose in the sample.

Description

HYGIENE MONITORING Field of the Invention
This invention relates to hygiene monitoring, and in particular to an assay for the presence of food residues and/or microorganisms. Background of the Invention
Hygiene monitoring procedures typically involve swabbing a surface, and analysing the sample taken up in the swab, for the presence of food residues and/or microorganisms. By way of example only, a typical hygiene monitoring device is disclosed in WO-A-98/27196. The ability to detect the presence of food residues and/or microorganisms in the sample that has been taken up by swabbing relies on the presence of ADP/ATP, and the ability to amplify the amount of ADP/ATP, and convert it to a detectable signal, also as disclosed in WO-A-94/25619. The preferred amplification reaction involves, inter alia, the amplification of ATP and then the conversion of glucose-6-phosphate to glucose, and the conversion of glucose, via a sequence of enzymatic reactions, to a coloured end point. Summary of the Invention
It has now been found that, in certain circumstances, glucose itself may be a sufficient indicator, for the purposes of an assay used in hygiene monitoring.
According to the present invention, therefore, such an assay comprises the collection of a sample from a locus, and the determination of the presence of glucose in the sample.
The reagents disclosed in WO-A-94/25619 include those sufficient to convert glucose to a detectable signal; if desired, the reagents used in this invention may exclude those that convert ATP, but the signal generated in the presence of glucose appears relatively rapidly, and can thus be distinguished, in addition to the advantage of providing a rapid response. The amplification of ATP needed to generate the signal is relatively slow. Description of the Invention
In use of the invention, it may desirable to ensure that the sample is collected from an environment that originally was, or has subsequently been tested and shown to be, free of sugar. It may also be desirable that the locus to be tested is also assayed for the substantial absence of materials that may interfere in the novel assay, e.g. by inhibiting or by giving false positives, such as peroxide or reducing agents that may be incorporated in materials used to sanitise the locus. The present invention can be practised utilising the same reagents as are disclosed in WO-A-94/25619, especially in so far as that relates to the conversion of glucose to a detectable signal. If desired, the reagents may exclude one or more of the components that are disclosed there for the conversion of ATP to glucose. A suitable device that can be used for the purposes of swabbing and detection is disclosed in WO-A-98/27196 and WO-A-99/31218.
The following -Examples illustrate the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619. Example 1
Swabs were taken from a variety of locations, and tested. In addition to determining the total viable count (TVC) and Enterobacteria (Enteros), contact plates were used alongside two devices. systemSURE ("sSURE") is a portable hygiene monitoring system (available from Becton Dickinson) that uses bioluminescence as an endpoint. The "Pen Swab" is generally as disclosed in WO-A-99/31218. Results are shown in Table 1.
Table 1
Figure imgf000004_0001
ND = no data
Inst = "Instant" colour change
Threshold - 500 RLU
The data show that, in samples 1 and 4, the colour reagents produced an instant positive signal which, when compared to the bioluminescence results, is an indication of the present of glucose and not ATP. Sample 2 also gave a high total viable count (over enumeration limit) and 75 counts for Enteros, while giving 625 RLU for systemSURE and an immediate colour change for the pen. This again indicates the presence of glucose leading to contamination. Example 2
The colour reagents were used to determine the level at which glucose was detectable. The reagents were activated in the presence of different concentrations of glucose. The absorbance values for a complete colour change were obtained within 120 seconds. Results are shown in Table 2.
Table 2
Figure imgf000005_0001
Swabs were taken from a variety of locations in a store, at different times of day and tested. Results are shown, for two different days, in Tables 3A and 3B. A large proportion of the data shows a rapid colour change (in seconds), characteristic of glucose detection. The corresponding RLU values do not show the presence of gross amounts of ATP, which is consistent with the detection of glucose. Table 3A
Figure imgf000006_0001
Table 3B
on
Figure imgf000007_0001

Claims

1. A hygiene monitoring method which comprises the collection of a sample from a locus, and the determination of the presence of glucose in the sample.
2. A method according to claim 1 , which comprises adding to the sample reagents that convert glucose to give a signal.
3. A method according to claim 2, wherein the reagents also convert ATP to give the signal, and the signal is generated more quickly in the presence of glucose than in the presence of ATP.
PCT/GB1999/004244 1998-12-15 1999-12-15 Hygiene monitoring WO2000036139A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000588387A JP2002532698A (en) 1998-12-15 1999-12-15 Hygiene monitoring
EP99961211A EP1141380A1 (en) 1998-12-15 1999-12-15 Hygiene monitoring
AU17897/00A AU1789700A (en) 1998-12-15 1999-12-15 Hygiene monitoring
US09/882,626 US20020037590A1 (en) 1999-12-15 2001-06-15 Hygiene monitoring

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9827584.5 1998-12-15
GBGB9827584.5A GB9827584D0 (en) 1998-12-15 1998-12-15 Hygiene Monitoring

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/882,626 Continuation-In-Part US20020037590A1 (en) 1999-12-15 2001-06-15 Hygiene monitoring

Publications (1)

Publication Number Publication Date
WO2000036139A1 true WO2000036139A1 (en) 2000-06-22

Family

ID=10844247

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/004244 WO2000036139A1 (en) 1998-12-15 1999-12-15 Hygiene monitoring

Country Status (5)

Country Link
EP (1) EP1141380A1 (en)
JP (1) JP2002532698A (en)
AU (1) AU1789700A (en)
GB (1) GB9827584D0 (en)
WO (1) WO2000036139A1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002055730A2 (en) * 2001-01-11 2002-07-18 Hygiena Llc Hygiene monitoring
US6924498B2 (en) 2002-04-24 2005-08-02 Biocontrol Systems, Inc. Sample collection and testing system
US7030403B2 (en) 2001-12-06 2006-04-18 Biocontrol Systems, Inc. Sample collection and bioluminescent sample testing system
US7229783B2 (en) 2000-08-01 2007-06-12 Charm Sciences, Inc. Method for monitoring hygiene
US9446406B2 (en) 2012-06-29 2016-09-20 Biocontrol Systems, Inc. Sample collection and bioluminescent analysis system

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025619A1 (en) * 1993-04-23 1994-11-10 Celsis Limited Detection of biological material
WO1997040381A1 (en) * 1996-04-19 1997-10-30 Xenogen Biodetectors targeted to specific ligands

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025619A1 (en) * 1993-04-23 1994-11-10 Celsis Limited Detection of biological material
WO1997040381A1 (en) * 1996-04-19 1997-10-30 Xenogen Biodetectors targeted to specific ligands

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7229783B2 (en) 2000-08-01 2007-06-12 Charm Sciences, Inc. Method for monitoring hygiene
WO2002055730A2 (en) * 2001-01-11 2002-07-18 Hygiena Llc Hygiene monitoring
WO2002055730A3 (en) * 2001-01-11 2003-05-15 Hygiena Llc Hygiene monitoring
US7030403B2 (en) 2001-12-06 2006-04-18 Biocontrol Systems, Inc. Sample collection and bioluminescent sample testing system
US7399984B2 (en) 2001-12-06 2008-07-15 Biocontrol Systems Inc. Sample collection and testing system having a displacement member causing reagent to come into contact with a sample collection surface
US7544961B2 (en) 2001-12-06 2009-06-09 Biocontrol Systems, Inc. Sample collection and testing system including a rotatable shaft with a helical guiding member to translate longitudinal motion of a slidable shaft into rotational motion
US6924498B2 (en) 2002-04-24 2005-08-02 Biocontrol Systems, Inc. Sample collection and testing system
US9446406B2 (en) 2012-06-29 2016-09-20 Biocontrol Systems, Inc. Sample collection and bioluminescent analysis system
US10684232B2 (en) 2012-06-29 2020-06-16 Biocontrol Systems, Inc. Sample collection and bioluminescent analysis system

Also Published As

Publication number Publication date
AU1789700A (en) 2000-07-03
EP1141380A1 (en) 2001-10-10
GB9827584D0 (en) 1999-02-10
JP2002532698A (en) 2002-10-02

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