WO2000036407A9 - Separation of charged particles by a spatially and temporally varying electric field - Google Patents

Separation of charged particles by a spatially and temporally varying electric field

Info

Publication number
WO2000036407A9
WO2000036407A9 PCT/US1999/029195 US9929195W WO0036407A9 WO 2000036407 A9 WO2000036407 A9 WO 2000036407A9 US 9929195 W US9929195 W US 9929195W WO 0036407 A9 WO0036407 A9 WO 0036407A9
Authority
WO
WIPO (PCT)
Prior art keywords
separation
potential
spatially
charged particles
temporally varying
Prior art date
Application number
PCT/US1999/029195
Other languages
French (fr)
Other versions
WO2000036407A1 (en
Inventor
Joel S Bader
Jonathan M Rothberg
Michael W Deem
Gregory T Mulhern
Gregory T Went
John Simpson
Steven Henck
Original Assignee
Curagen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Curagen Corp filed Critical Curagen Corp
Priority to EP99966079A priority Critical patent/EP1147409A4/en
Priority to JP2000588596A priority patent/JP2002532715A/en
Priority to AU21711/00A priority patent/AU2171100A/en
Priority to CA002354589A priority patent/CA2354589A1/en
Publication of WO2000036407A1 publication Critical patent/WO2000036407A1/en
Publication of WO2000036407A9 publication Critical patent/WO2000036407A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites

Abstract

This invention relates to a method and device for separating particles according to their diffusivities in a separation medium by means of a spatially and temporally varying electric potential. The method takes advantage of the transport of charged particles subject to an electric potential that is cycled between an off-state (in which the potential is flat) and one or more on-states, in which the potential is preferably spatially periodic with a plurality of eccentrically shaped stationary wells. The potential wells are at a constant spatial positions in the on-state. Differences in liquid-phase diffusivities lead to charged particle separation. A separation medium fills physically defined separation lanes (15) in the device. Electrodes deposited substantially transverse to the lanes create the required potential. Advantageously, injection ports (16) allow sample loading, and special gating electrodes focus the sample prior to separation.
PCT/US1999/029195 1998-12-16 1999-12-09 Separation of charged particles by a spatially and temporally varying electric field WO2000036407A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP99966079A EP1147409A4 (en) 1998-12-16 1999-12-09 Separation of charged particles by a spatially and temporally varying electric field
JP2000588596A JP2002532715A (en) 1998-12-16 1999-12-09 Separation of charged particles by spatially and temporally varying electric fields
AU21711/00A AU2171100A (en) 1998-12-16 1999-12-09 Separation of charged particles by a spatially and temporally varying electric field
CA002354589A CA2354589A1 (en) 1998-12-16 1999-12-09 Separation of charged particles by a spatially and temporally varying electric field

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/212,622 US6193866B1 (en) 1996-03-27 1998-12-16 Separation of charged particles by a spatially and temporally varying electric field
US09/212,622 1998-12-16

Publications (2)

Publication Number Publication Date
WO2000036407A1 WO2000036407A1 (en) 2000-06-22
WO2000036407A9 true WO2000036407A9 (en) 2000-12-07

Family

ID=22791795

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/029195 WO2000036407A1 (en) 1998-12-16 1999-12-09 Separation of charged particles by a spatially and temporally varying electric field

Country Status (6)

Country Link
US (1) US6193866B1 (en)
EP (1) EP1147409A4 (en)
JP (1) JP2002532715A (en)
AU (1) AU2171100A (en)
CA (1) CA2354589A1 (en)
WO (1) WO2000036407A1 (en)

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Also Published As

Publication number Publication date
EP1147409A1 (en) 2001-10-24
CA2354589A1 (en) 2000-06-22
WO2000036407A1 (en) 2000-06-22
AU2171100A (en) 2000-07-03
US6193866B1 (en) 2001-02-27
EP1147409A4 (en) 2002-09-11
JP2002532715A (en) 2002-10-02

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