WO2000044935A3 - Multiplex real-time pcr - Google Patents

Multiplex real-time pcr Download PDF

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Publication number
WO2000044935A3
WO2000044935A3 PCT/EP2000/000677 EP0000677W WO0044935A3 WO 2000044935 A3 WO2000044935 A3 WO 2000044935A3 EP 0000677 W EP0000677 W EP 0000677W WO 0044935 A3 WO0044935 A3 WO 0044935A3
Authority
WO
WIPO (PCT)
Prior art keywords
probe
variants
estimate
time pcr
viral load
Prior art date
Application number
PCT/EP2000/000677
Other languages
French (fr)
Other versions
WO2000044935A2 (en
Inventor
Dieter Klein
Walter Guenzburg
Brian Salmons
Original Assignee
Bavarian Nordic Res Inst As
Dieter Klein
Walter Guenzburg
Brian Salmons
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bavarian Nordic Res Inst As, Dieter Klein, Walter Guenzburg, Brian Salmons filed Critical Bavarian Nordic Res Inst As
Priority to AU27994/00A priority Critical patent/AU2799400A/en
Priority to JP2000596175A priority patent/JP2002539769A/en
Priority to CA002354184A priority patent/CA2354184A1/en
Priority to EP00906242A priority patent/EP1147225A1/en
Publication of WO2000044935A2 publication Critical patent/WO2000044935A2/en
Priority to US09/919,501 priority patent/US20030087397A1/en
Publication of WO2000044935A3 publication Critical patent/WO2000044935A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

The present invention relates to a real-time Polymerase Chain Reaction (PCR) method for the detection and quantification of variants of nucleic acid sequences which differ in the probe-bindung site. The method is based on the complete or partial amplification of the same region of the variants and the addition of two or more oligonucleotide probes to the same PCR mixture, each probe being specific for the probe-binding site of at least one variant. The method can be applied e.g. to estimate the viral load in a sample, to differentiate between subgroups, subtypes isolates or clades of a viral species or to estimate the impact of the viral load on tumorigenesis.
PCT/EP2000/000677 1999-01-29 2000-01-28 Multiplex real-time pcr WO2000044935A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU27994/00A AU2799400A (en) 1999-01-29 2000-01-28 Multiplex real-time pcr
JP2000596175A JP2002539769A (en) 1999-01-29 2000-01-28 Multiplex real-time PCR
CA002354184A CA2354184A1 (en) 1999-01-29 2000-01-28 Multiplex real-time pcr
EP00906242A EP1147225A1 (en) 1999-01-29 2000-01-28 Multiplex real-time pcr
US09/919,501 US20030087397A1 (en) 1999-01-29 2001-07-30 Multiplex real-time PCR

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199900114 1999-01-29
DKPA199900114 1999-01-29

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/919,501 Continuation-In-Part US20030087397A1 (en) 1999-01-29 2001-07-30 Multiplex real-time PCR

Publications (2)

Publication Number Publication Date
WO2000044935A2 WO2000044935A2 (en) 2000-08-03
WO2000044935A3 true WO2000044935A3 (en) 2001-10-04

Family

ID=8089913

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/000677 WO2000044935A2 (en) 1999-01-29 2000-01-28 Multiplex real-time pcr

Country Status (6)

Country Link
US (1) US20030087397A1 (en)
EP (1) EP1147225A1 (en)
JP (1) JP2002539769A (en)
AU (1) AU2799400A (en)
CA (1) CA2354184A1 (en)
WO (1) WO2000044935A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6300075B1 (en) * 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
NZ575481A (en) * 2000-10-18 2010-10-29 Pharmasset Inc Simultaneous quantification of nucleic acids in diseased cells
RU2004100112A (en) * 2001-06-06 2005-04-20 Канаг Диагностикс Аб (Se) METHOD FOR MEASURING THE RELATIONSHIP OF GENE EXPRESSION OF KEY GENES
JP4148900B2 (en) * 2002-01-08 2008-09-10 エフ．ホフマン−ラ ロシュ アーゲー Use of silica materials in amplification reactions
US20040161767A1 (en) * 2002-06-28 2004-08-19 Baldwin Brett R. Detection and quantification of aromatic oxygenase genes by real-time PCR
EP2031070B1 (en) 2002-12-04 2013-07-17 Life Technologies Corporation Multiplex amplification of polynucleotides
CN100334209C (en) * 2002-12-30 2007-08-29 徐定邦 PCR method using two groups of homologous primer and its reaction liquid and use
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
WO2005123756A1 (en) * 2004-06-21 2005-12-29 University Of Guelph A method of diagnosing fiv
CA2871777C (en) 2005-03-10 2015-07-28 Matthew J. Hayes System and methods for detecting multiple optical signals
ATE553220T1 (en) * 2006-03-13 2012-04-15 Wako Pure Chem Ind Ltd METHOD FOR DETECTING A MUTATED GENE
JP5601746B2 (en) * 2006-03-16 2014-10-08 日本ソフトウェアマネジメント株式会社 Two-step nucleic acid test method using the same sample
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
US9284613B2 (en) 2010-10-14 2016-03-15 Rheonix, Inc. Quantitative multiplexed identification of nucleic acid targets
WO2012116308A1 (en) 2011-02-24 2012-08-30 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
WO2014160233A1 (en) * 2013-03-13 2014-10-02 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
RU2553534C1 (en) * 2014-07-22 2015-06-20 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Саратовский государственный аграрный университет имени Н.И. Вавилова" Pair of synthetic oligonucleotide primers for detection of virus of immunodeficiency of cats and method of diagnostics of virus of immunodeficiency of cats

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040268A1 (en) * 1995-06-07 1996-12-19 American Home Products Corporation Recombinant raccoon pox viruses and their use as an effective vaccine against feline immunodeficiency virus infection

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040268A1 (en) * 1995-06-07 1996-12-19 American Home Products Corporation Recombinant raccoon pox viruses and their use as an effective vaccine against feline immunodeficiency virus infection

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GERARD ET AL: "IMPROVED QUANTITATION OF MINIMAL RESIDUAL DISEASE IN MULTIPLE MYELOMA USING REAL-TIME POLYMERASE CHAIN REACTION AND PLASMID-DNA COMPLEMENTARITY DETERMINING REGION III STANDARDS", CANCER RESEARCH,US,AMERICAN ASSOCIATION FOR CANCER RESEARCH, BALTIMORE, MD, vol. 58, 1 September 1998 (1998-09-01), pages 3957 - 3964, XP002095189, ISSN: 0008-5472 *
HEID C A ET AL: "REAL TIME QUANTITATIVE PCR", GENOME RESEARCH,US,COLD SPRING HARBOR LABORATORY PRESS, vol. 6, no. 10, 1 October 1996 (1996-10-01), pages 986 - 994, XP000642795, ISSN: 1088-9051 *
KLEIN D ET AL.: "Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: Influence of mismatches on quantification", ELECTROPHORESIS, vol. 20, 1999, pages 291 - 299, XP000924950 *
LETTENEGGER C ET AL: "Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TAQMAN.RTM. fluorogenic real-time detection system", JOURNAL OF VIROLOGICAL METHODS,NL,AMSTERDAM, vol. 78, no. 78, January 1999 (1999-01-01), pages 105 - 116, XP002103560, ISSN: 0166-0934 *
VAHLENKAMP T W ET AL: "COMPETITIVE REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION FOR QUANTITATION OF FELINE IMMUNODEFICIENCY VIRUS", JOURNAL OF VIROLOGICAL METHODS,NL,AMSTERDAM, vol. 52, no. 3, 1 April 1995 (1995-04-01), pages 335 - 346, XP000609892, ISSN: 0166-0934 *
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Also Published As

Publication number Publication date
US20030087397A1 (en) 2003-05-08
AU2799400A (en) 2000-08-18
WO2000044935A2 (en) 2000-08-03
JP2002539769A (en) 2002-11-26
EP1147225A1 (en) 2001-10-24
CA2354184A1 (en) 2000-08-03

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