WO2000067086A1 - Synthesizing combinatorial libraries of materials - Google Patents

Synthesizing combinatorial libraries of materials Download PDF

Info

Publication number
WO2000067086A1
WO2000067086A1 PCT/US2000/012228 US0012228W WO0067086A1 WO 2000067086 A1 WO2000067086 A1 WO 2000067086A1 US 0012228 W US0012228 W US 0012228W WO 0067086 A1 WO0067086 A1 WO 0067086A1
Authority
WO
WIPO (PCT)
Prior art keywords
user
programmed code
mapping
procedure
operable
Prior art date
Application number
PCT/US2000/012228
Other languages
French (fr)
Inventor
William C. Rust
Ralph B. Nielson
Original Assignee
Symyx Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23182549&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2000067086(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Symyx Technologies, Inc. filed Critical Symyx Technologies, Inc.
Priority to DK00928837T priority Critical patent/DK1175645T3/en
Priority to EP00928837A priority patent/EP1175645B1/en
Priority to AT00928837T priority patent/ATE245286T1/en
Priority to DE60003913T priority patent/DE60003913T2/en
Priority to JP2000615862A priority patent/JP2002542943A/en
Priority to CA002370573A priority patent/CA2370573A1/en
Priority to AU47013/00A priority patent/AU4701300A/en
Publication of WO2000067086A1 publication Critical patent/WO2000067086A1/en

Links

Classifications

    • GPHYSICS
    • G05CONTROLLING; REGULATING
    • G05BCONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
    • G05B19/00Programme-control systems
    • G05B19/02Programme-control systems electric
    • G05B19/04Programme control other than numerical control, i.e. in sequence controllers or logic controllers
    • G05B19/042Programme control other than numerical control, i.e. in sequence controllers or logic controllers using digital processors
    • G05B19/0426Programming the control sequence
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068Means for controlling the apparatus of the process
    • B01J2219/00686Automatic
    • B01J2219/00689Automatic using computers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068Means for controlling the apparatus of the process
    • B01J2219/00686Automatic
    • B01J2219/00691Automatic using robots
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068Means for controlling the apparatus of the process
    • B01J2219/00695Synthesis control routines, e.g. using computer programs

Definitions

  • the present invention relates to methods and apparatus for controlling automated material-handling procedures.
  • Combinatorial materials science refers generally to methods for creating a collection (or "library") of chemically diverse compounds or materials and/or to methods for rapidly testing or screening such libraries for desirable performance characteristics and properties.
  • combinatorial techniques accelerate the speed of research, facilitate breakthroughs and expand the amount of information available to researchers.
  • the ability to examine the relationships between hundreds or thousands of materials in a short period of time enables scientists to make well-informed decisions in the discovery process and to identify unexpected trends.
  • Implementation of combinatorial screening methods depends on the ability to prepare libraries of materials using combinatorial synthetic techniques. These techniques typically employ a robot to automate library preparation and screening, and computer programs have been used to control such robots.
  • the invention features methods and apparatus, including computer program products, operable to control automated material handling procedures.
  • the invention is particularly useful for controlling the automated synthesis and/or screening of combinatorial libraries, but is generally useful for controlling material handling procedures involving the transfer of materials from one or more source locations to one or more destination locations.
  • the invention features a computer program on a computer-readable medium for controlling a material-handling apparatus.
  • the program includes instructions operable to cause a programmable processor to provide an assortment of pre-programmed code objects; and receive from a user a selection and arrangement of pre-programmed code objects from the assortment.
  • the assortment includes a first pre-programmed code object operable to receive an input defining one or more source components, one or more destination components and one or more mappings. Each mapping relates a source component and a destination component and defines one or more transfers of at least one source component material to at least one destination component locations.
  • the selection defines a set of material handling steps.
  • the arrangement defines an order of execution for the selected pre-programmed code objects.
  • the first pre-programmed code object is operable to interact with a user to receive a mapping.
  • the first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations and the destination component with a destination location including an access port of an analytical device.
  • the input is a recipe file including library design information defining the source component, the destination component and the mapping.
  • the material handling steps define a procedure for synthesizing a combinatorial library of materials.
  • the mapping defines a gradient for distributing the source component materials among the destination locations.
  • the first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations, each of which has a source component material, and the destination component with a plurality of destination locations corresponding to a plurality of members of the combinatorial library.
  • the assortment includes at least one second pre-programmed code object operable to label a mapping for processing by at least one third pre-programmed code object to be executed subsequently in the arrangement.
  • the arrangement includes a logical hierarchy including a parent pre-programmed code object and at least one child pre-programmed code object.
  • the parent pre-programmed code object is operable to define a condition determining whether the at least one child pre-programmed code object will be executed subsequently in the arrangement.
  • the parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each mapping defined by the input.
  • the parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each transfer defined by one of the one or more mappings.
  • the condition is a "for" condition.
  • the condition is a "while” condition.
  • the condition is an "if condition.
  • the invention features a computer program for controlling an automated material-handling apparatus for carrying out a set of material-handling steps.
  • the program includes instructions operable to cause a programmable processor to receive a recipe file comprising component information and mapping information; interact with a user to create a procedure for executing the set of material handling steps; and cause the automated material-handling apparatus to carry out the set of material -handling steps by executing the procedure.
  • the mapping information relates a source component and a destination component.
  • the mapping information defines one or more transfers of at least one source component material to at least one destination component location.
  • the program includes instructions to provide to the user an assortment of pre-programmed code objects and receive from the user a selection and arrangement of the pre-programmed code objects.
  • the procedure is defined by the user's selection and arrangement.
  • the procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file.
  • Implementations of the invention can include one or more of the following advantageous features.
  • the program includes instructions operable to cause a programmable processor to interact with a user to create a resource hierarchy including one or more resource objects defining at least one hardware device of the automated material-handling apparatus coupled to the programmable processor.
  • the program includes instructions operable to cause a programmable processor to interact with a user to create a system hierarchy including one or more system objects defining one or more robot arms coupled to the at least one hardware device of the automated material-handling apparatus.
  • the program includes instructions operable to cause a programmable processor to interact with a user to create a substrate hierarchy including one or more substrate objects defining a geometry for at least one substrate.
  • the program includes instructions operable to cause a programmable processor to interact with a user to create a one or more position objects for each substrate object in the substrate hierarchy.
  • At least one substrate object corresponds to one or more source locations.
  • At least one substrate object corresponds to one or more destination locations.
  • Execution of the procedure translates the component information and the mapping information to machine level commands for controlling the automated material-handling apparatus.
  • the program includes instructions operable to enable the user to modify the procedure during execution.
  • the invention features a computer-implemented method for controlling a material-handling apparatus.
  • the method includes providing an assortment of pre-programmed code objects; and receiving from a user a selection and arrangement of pre-programmed code objects from the assortment.
  • the assortment includes a first pre-programmed code object operable to receive an input defining one or more source components, one or more destination components and one or more mappings. Each mapping relates a source component and a destination component and defines one or more transfers of at least one source component material to at least one destination component locations.
  • the selection defines a set of material handling steps.
  • the arrangement defines an order of execution for the selected pre-programmed code objects.
  • the first pre-programmed code object is operable to interact with a user to receive a mapping arrangement which defines a material-handling procedure for execution by the material-handling apparatus.
  • the first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations and the destination component with a destination location comprising an access port of an analytical device.
  • the input is a recipe file including library design information defining the source component, the destination component and the mapping.
  • the material handling steps define a procedure for synthesizing a combinatorial library of materials.
  • the mapping defines a gradient for distributing the source component materials among the destination locations.
  • the first pre-programmed code object is operable to interact with a user to associate the source component with one or more source location, each of which has a source component material, and the destination component with a plurality of destination locations corresponding to a plurality of members of the combinatorial library.
  • the assortment includes at least one second pre-programmed code object operable to label a mapping for processing by at least one third pre-programmed code object to be executed subsequently in the arrangement.
  • the arrangement includes a logical hierarchy including a parent pre-programmed code object and at least one child pre-programmed code object.
  • the parent pre-programmed code object is operable to define a condition determining whether the at least one child pre-programmed code object will be executed subsequently in the arrangement.
  • the parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each mapping defined by the input.
  • the parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each transfer defined by one of the one or more mappings.
  • the condition is a "for" condition.
  • the condition is a "while” condition.
  • the condition is an "if condition.
  • the invention features a method of controlling an automated material-handling apparatus for executing a set of material-dispensing steps.
  • the method includes receiving a recipe file comprising component information and mapping information; interacting with a user to create a procedure for synthesizing the combinatorial library; and causing the automated material-handling apparatus to synthesize the combinatorial library by executing the procedure.
  • the mapping information relates a source component and a destination component.
  • the mapping information defines one or more transfers of at least one source component material to at least one destination component location.
  • the method includes providing to the user an assortment of pre-programmed code objects and receiving from the user a selection and arrangement of the pre-programmed code objects.
  • the procedure is defined by the user's selection and arrangement.
  • the procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file.
  • Implementations of the invention can include one or more of the following advantageous features.
  • the method includes interacting with a user to create a resource hierarchy including one or more resource objects defining at least one hardware device of the automated material-handling apparatus coupled to the programmable processor.
  • the method includes interacting with a user to create a system hierarchy including one or more system objects defining one or more robot arms coupled to the at least one hardware device of the automated material-handling apparatus.
  • the method includes interacting with a user to create a substrate hierarchy including one or more substrate objects defining a geometry for at least one substrate.
  • the method includes interacting with a user to create a one or more position objects for each substrate object in the substrate hierarchy. At least one substrate object corresponds to one or more source locations. At least one substrate object corresponds to one or more destination locations. Execution of the procedure translates the component information and the mapping information to machine level commands for controlling the automated material-handling apparatus. The method includes modifying the procedure during execution.
  • the invention features a system for executing a set of material-handling steps.
  • the system includes means for receiving a recipe file comprising component information and mapping information; means for interacting with a user to create a procedure for synthesizing a combinatorial library of materials; and means for synthesizing the combinatorial library by executing the procedure.
  • the mapping information relates a source component and a destination component and defining one or more transfers of at least one source component material to at least one destination component location.
  • the means for interacting with the user includes means for providing to the user an assortment of pre-programmed code objects and means for receiving from the user a selection and arrangement of the pre-programmed code objects.
  • the procedure is defined by the user's selection and arrangement.
  • the procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file.
  • code blocks allows users to create highly customized procedures for a wide variety of material -handling processes including preparation or synthesis of combinatorial libraries as well as characterization of libraries of materials. New code blocks can be generated and collections created storing code blocks for use in future procedures. Procedures can be changed on the fly to allow experimentation and process exploration. Procedures are not restricted to specific hardware.
  • code blocks in a graphical programming environment allows users to create procedures without requiring knowledge of complicated programming languages. Procedures can be run with minimal user interaction during execution.
  • high level library design data allows the synthesis of libraries designed in different design environments.
  • FIG. 1 is a block diagram illustrating a system for controlling an automated material handling procedure.
  • FIG. 2 is a flow diagram generally illustrating a method of controlling an automated material handling procedure.
  • FIG. 3 is a flow diagram illustrating a method of controlling an automated material handling procedure in more detail.
  • FIG. 4 is an example of a dialog window displaying a procedure generated according to the claimed invention.
  • FIG. 5 is a flow diagram illustrating a method of generating a procedure.
  • FIG. 6 is an example of a dialog window displaying a list of procedures and an event log according to the claimed invention.
  • FIG. 7 is a window illustrating a procedure for processing multiple tagged mappings.
  • FIG. 8 is a window illustrating a material-handling procedure for characterizing a library of materials.
  • the instructions are obtained from a recipe file for the automated synthesis of a combinatorial library of materials.
  • Materials can include any type of chemical compound, mixture or other material of interest.
  • the combinatorial library of materials is prepared by placing components in a destination, such as a microtiter plate, silicon wafer or the like. Each destination includes an array of regions, which may include, for example, the wells of a microtiter plate or locations on a wafer.
  • the resulting set of materials is the library, which includes a set of "members" corresponding to materials at each region of the destination array.
  • a library can be arranged in any convenient geometric shape, such as a square, rectangle, circle or triangle.
  • a set of material handling instructions is generated in design module 130 and provided to control module 140 (step 200).
  • control module 140 retrieves instructions from memory 150. Suitable instructions can be provided in a
  • Recipe file can include, for example, component information identifying source and destination components (representing physical containers such as vials or microtiter plates or physical locations such as regions on a substrate wafer) to be used in the synthesis of a library, mapping information relating each source to one or more destination components and identifying amounts of each source to be used to synthesize each library member, and parameters defining reaction conditions for the combination of components to form individual library members.
  • component information identifying source and destination components representing physical containers such as vials or microtiter plates or physical locations such as regions on a substrate wafer
  • mapping information relating each source to one or more destination components and identifying amounts of each source to be used to synthesize each library member
  • parameters defining reaction conditions for the combination of components to form individual library members for example, component information identifying source and destination components (representing physical containers such as vials or microtiter plates or physical locations such as regions on a substrate wafer) to be used in the synthesis of a library, mapping information relating each source to one or more destination components
  • Mapping information can include, for example, a list of specific amounts of materials to be transferred from one or more source vials to each of a set of destination wells. Mapping information can also include gradients specifying a range of amounts of a material or materials to be transferred from a particular source to a series of wells, with the amount to be transferred to each destination well determined according to a linear, exponential, logarithmic, polynomial, geometric or other mathematical expression.
  • the creation of recipe files is described in co-pending U.S. Application Serial No. 09/174,856, filed on October 19, 1998, which is incorporated herein by reference in its entirety.
  • Control module 140 captures the component, mapping and parameter information from a recipe file and makes the information available for use by user-defined procedures as will be described below.
  • a typical user of system 100 is a chemist.
  • the user initializes system 100 and creates a procedure using object editor 170 (step 210).
  • Control module 140 executes the procedure (step 220), causing material handling apparatus 180 to, for example, synthesize a combinatorial library specified by the set of instructions in the recipe file.
  • control module 140 communicates directly with apparatus 180, for example, by transmitting text commands through a serial port or ports to apparatus 180.
  • commands generated by module 140 can be implemented by drivers for apparatus 180 installed on computer 110.
  • Appropriate apparatus 180 can include, for example, automated liquid handling robots, such as the RSP 900 Robotic Sample Processor, available from Cavro Scientific Instruments, Inc. of Sunnyvale, California, or equivalents. Apparatus 180 can also include automated systems performing different types of physical or chemical vapor deposition.
  • liquid handling mixtures of solutions are typically dispensed in an array of miniature wells to create a library.
  • vacuum deposition solid elements or chemicals or mixtures of solid elements or chemicals are vaporized and deposited as individual components on a substrate. The deposition may be controlled by a series of shutters and masks to manufacture the library.
  • WO98/47613 U.S. Application Serial No. 08/841 ,423
  • step 300, 310, 320 and 330 the user employs object editor 170 to create and configure data objects defining the hardware and processes for the desired synthesis.
  • Each data object has an associated set of properties.
  • the user selects and inserts data objects into a hierarchy of objects defining the hardware and processes for the system, and customizes the selected objects by manipulating the properties associated with each object as will be described in more detail below.
  • Data objects are generated by the editor 170 through the use of computer programming techniques conventional in object-oriented programming. The editor is advantageously implemented to have a graphical user interface.
  • user interface 160 displays window 400 including an object hierarchy or tree 403 (in this case for procedure objects 405 and 408) in pane 410.
  • the user manipulates the properties of selected objects — for example, properties 414 and 418 of selected For Loop object 432 — by entering text or selecting values in the corresponding fields of pane 420, in this example, fields 424 and 428.
  • the user need not have any familiarity with the generation of the data objects themselves, or of the object-oriented programming techniques used to generate the data objects, in order to manipulate data objects as described below.
  • the user defines the physical hardware resources available to system 100 through user interface 160 (step 300).
  • appropriate hardware resources includes devices such as automated synthesis robots, syringe pump controllers, physical and vapor deposition systems and the like.
  • Hardware resources can also include temperature controllers for regulating the temperature of one or more source or destination vials or plates, syringes or other dispensing apparatus.
  • the user defines each hardware device attached to system 100 by creating a hardware resource data object specifically configured for the device in question, for example, specifying a serial port through which the device is attached to computer 110.
  • Each hardware resource object implements a protocol for communication between computer 110 and the particular device in question, giving computer 110 (and the user) access to the device's functionality.
  • the user defines the properties of the specific arms, syringes, shutters, masks or other devices installed in system 100 by creating system data objects for each such device (step 310).
  • Each system object identifies a hardware resource selected from those already defined (e.g., a particular robot or pump controller) that will control the device in question.
  • Other system object properties include the device address, the configuration of syringes attached to an arm (e.g., in series or in parallel), motion limits, step size and reference positions for arm movements, and resolution, volume and dispensing characteristics for syringes and the like.
  • the user also identifies each source of starting materials and each destination by creating a substrate data object defining the geometry for each single-well or multi-well substrate to be accessed by system 100; specific positions for these substrates on the work surface are defined using position objects, which the user inserts as child objects of the respective substrate object (step 320).
  • position objects which the user inserts as child objects of the respective substrate object (step 320).
  • user-defined properties include, for example, the vial's cross-sectional area and height, as well as the position at which the system will dispense liquid into the vial.
  • a vial position object associated with each vial defines the coordinates of the vial's location on the work surface.
  • Properties for two-dimensional substrate arrays include, for example, all of those associated with the single well substrate, as well as the number of rows and columns and the relevant spacing.
  • a plate position object defines the location of the two-dimensional array on the work surface by specifying, for example, grid coordinates of opposite corners of the array.
  • the user creates a procedure object (step 330) by assembling a hierarchy of code objects that can be executed by control module 140 and robot 180.
  • the process of using object editor 170 to create a liquid-dispensing procedure is described in reference to FIG. 5.
  • the user creates a procedure object using object editor 170 (step 500).
  • the user selects a code object for performing a desired function — such as loading synthesis instructions, initializing one or more robot arms, setting parameters for arm and syringe movement, moving arms, aspirating or dispensing liquids with syringes and the like — from a set of objects accessible by object editor 170 and inserts the object at the appropriate location in the procedure object's logical hierarchy (step 510).
  • container objects are containers used to group other objects into functional units, which can then be saved, copied and inserted into other procedures as a unit.
  • Container objects can include conditional "For Loop", "If and "While Loop” objects including one or more functional descendant or child objects that are executed zero, one or more times in a procedure according to the occurrence of a specified condition, as is described in more detail below.
  • Table 1 An example of a selection of suitable code objects is provided in Table 1, below. Table 1
  • Loop Loop Type Mapping Dispense or Numeric. If Mapping, object iterates through mappings loaded on system; if Dispense, object iterates through each dispense within current mapping; if Numeric, object iterates through specified integer range.
  • mapping Tag Specifies tag to match when iterating through mappings; only mappings with specified tag included in loop
  • Substrate Destination substrate selected from list of substrates defined for system
  • Position Position of destination substrate on work surface selected from list of positions defined for selected substrate
  • Relative X, Y Relative amounts to move arm in X, Y and Z & Z
  • the user defines the properties associated with the chosen code object (step 520). If additional code objects are required to complete the procedure (the YES branch of step 30), the user selects the next desired code object (step 510) and repeats the process.
  • the procedure is complete.
  • the user can validate the code to verify the consistency of the procedure, or any selected code object and its descendants (step 540).
  • Object editor 170 stores the procedure in memory 150 and/or provides the procedure to control module 140 for execution by apparatus 180 (step 550).
  • Procedure object 405 which the user has named "Simple Mapping w/ Left Arm" includes tree 403 of code objects 430-451. Each code object in tree 403 is represented by an icon, for example icon 460, which corresponds to the object type, and by a name, which can be a default name based on the object type or a name chosen by the user.
  • Procedure 405 includes a series of code objects that, when executed, cause control module 140 to instruct apparatus 180 to carry out a simple mapping procedure with the left arm of a synthesis robot. Control module 140 executes procedure 405 as follows.
  • "Initialize Arm” object 430 initializes an arm of the robot, setting the arm and syringe speeds and the syringe maximum aspirate and minimum dispense volumes to default values.
  • "Load Recipe” object 431 loads component, mapping and parameter information from a specified recipe file. As described above, a suitable recipe file contains component information identifying the source and destination components to used during execution of the procedure, as well as mapping information specifying amounts to be transferred from each source component to one or more destination components. The recipe file can also contain parameter information, in the form of values or sets of values associated with components identified in the recipe file.
  • Execution of "Load Recipe" object 431 causes control module 140 to prompt the user for the name of a recipe file to load.
  • a specific recipe file can be identified as a property of "Load Recipe” object 431.
  • Control module 140 loads the specified recipe file and causes user interface 160 to display tables of component and mapping information.
  • Control module 140 prompts the user to identify and provide a position on the work surface for each substrate component defined in step 320, discussed above.
  • a given substrate can be used as a source (e.g., a location from which a reagent is transferred), a destination (a location to which a reagent is transferred), or both (e.g., a location in which two or more reagents are mixed and the mixture transferred to another location) during execution of a procedure.
  • Any parameter data in the recipe file is associated with the specified substrate objects, and is made available to subsequent code objects in procedure 405.
  • the recipe file loaded by object 431 contained "Temperature” parameter information including a value for each well in a specified destination plate, those values would be available to subsequent code objects invoking a predefined "Temperature” variable defined using a "Define Variable” object as set out in Table 1 and discussed in more detail below.
  • a set of "Temperature” parameters can be advantageously invoked by "Set Temperature”, “Get Temperature” and "Wait for Temperature” objects as set out in Table 1.
  • "For Each Mapping" object 432 is a container object — specifically a For Loop object ⁇ that executes its set of child objects for a specified number of iterations.
  • object 432 is a "mapping loop” that iterates over each mapping in the recipe file loaded by "Load Recipe” object 431.
  • Object 432 causes control module 140 to define a "Total Mappings" variable that contains the total number of mappings loaded from the recipe file, and a "Mapping Number” variable that is set to the index of the current mapping and ranges from one to the value of Total Mappings. Both of these variables are available to all child objects of loop object 432.
  • the child objects of loop 432 are objects 433-450, which are executed by control module 140 for each loaded mapping.
  • "For Each Dispense" object 433 is another For Loop object, this time iterating through each dispense specified in the current mapping (corresponding to the mapping identified by the Mapping Number variable discussed above).
  • Object 433 causes control module 140 to define eight system variables available to all child objects of loop object 433. These include a "Total Dispenses" variable containing the number of dispenses for the current mapping and a "Dispense Number” variable containing the index of the current dispense and ranging from one to the value of "Total Dispenses".
  • These variables also include a "Mapping Aspirate Volume” variable containing the volume to aspirate (or retrieve from a source component), based on the mapping destination well (or on multiple wells based on a predetermined "look ahead interval” specifying a set number of wells to be considered when calculating the amount to aspirate) and a “Mapping Dispense Volume” variable containing the volume to dispense for the current mapping destination; for each dispense, both of these variables are initialized to preset values for the dispense and are updated as the dispense progresses.
  • object 433 also causes control module 140 to define "Source Row” and "Source Column” variables indicating the row and column of the current source well and "Destination Row” and “Destination Column” variables indicating the row and column of the current destination well.
  • the child objects for loop object 433 are objects 434-441, which are repeated in sequence for each dispense in each loaded mapping.
  • object 434 is another type of container object ⁇ a While Loop object — that causes all child objects to be executed repeatedly as long as a specified condition is true.
  • object 434 causes control module 140 to determine whether the value of the Mapping Dispense Volume variable is greater than zero, indicating that the dispense still calls for liquid to be dispensed. For as long as this expression evaluates to true, control module 140 executes child objects 435-441. When the expression evaluates to false (i.e., no volume remains to be dispensed), the current dispense is complete and control module 140 proceeds to the next iteration of "For Each Dispense" loop 433.
  • While object 434 can implement any convenient logical expression, such as arithmetic expressions comparing one value with another value. While Loop object 434 can also implement "syringe conditions" that evaluate whether a given syringe needs to be refilled, or "mapping tag” conditions that evaluate whether the current mapping has been identified for particular action by the user. "Fill Syringe” object 435 is still another container object ⁇ an If object — that causes control module 140 to execute its child objects if a specified condition is true. In this case, object 435 causes control module 140 to determine whether the specified syringe must be refilled (i.e., when a "syringe empty” condition evaluates to "true”).
  • object 435 causes control module 140 to execute "Move to Source” object 436, a Move Arm object that instructs apparatus 180 to move the syringe to the location of the specified source component, and Aspirate object 437, which instructs the syringe to aspirate from that source component an amount determined by the "Mapping Aspirate Volume” variable defined for the current dispense.
  • "Move to Destination” object 438 (another Move Arm object) causes apparatus 180 to move the loaded syringe to the location of the specified destination component.
  • "Dispense” object 439 causes apparatus 180 to dispense into the specified destination well an amount determined by the Mapping Dispense Volume variable defined for the current dispense.
  • "Set Timer” and “Wait For Timer” objects 440 and 441 cause control module 140 to pause for a preset (or variable) period of time before proceeding to the next dispense iteration. After completing all dispenses for the current mapping (and after each successive mapping), control module 140 executes Container object 442, named "Standard Wash - Left 500ul".
  • "Move To Waste” object 443, another Move Arm object, causes apparatus 180 to move the syringe to the location of a "waste" destination.
  • "Purge Syringe” object 444 causes apparatus 180 to empty all contents of the specified syringe into the waste destination.
  • "Dispense” object 445 causes apparatus 180 to refill the specified syringe with a specified volume from a reservoir (typically filled with a solvent such as, for example, water) and dispense the specified volume.
  • Control module 140 causes apparatus 180 to move the specified syringe to a cleaning station ("Move To Clean 1" object 446), where the syringe is again filled with solvent, which is dispensed (Dispense object 447).
  • "Move To Clean 2" object 448 causes apparatus 180 to move the specified syringe to a second cleaning station, for a final wash ("Dispense” object 449).
  • FIG. 6 shows a user interface window 600.
  • the user instructs control module 140 to execute a completed procedure, for example by activating "Execute" button 620 in status pane 610 of window 600, which causes user interface 160 to display a list of available procedures in computer 110.
  • Status pane 610 includes a line item 630 indicating that control module 140 is executing a procedure. While a procedure executes, user interface 160 displays status messages in event log 640. The user can pause or abort a procedure during execution by selecting the appropriate line item and activating button 650 or 660, respectively.
  • event log 640 can provide a record of high level events, or it can provide a detailed record of each step in a procedure. Event log 640 also displays any errors occurring during execution.
  • control module 140 pauses the executing procedure and posts the error to event log 640. While the procedure is paused, the user can execute other procedures in order to correct the error. For example, if the error resulted from a mistrained substrate position, the user can attempt to retrain the position. The user can also modify the paused procedure itself using object editor 170. When the error has been corrected, the user instructs control module
  • Control module 140 to resume execution of the original procedure by again activating "pause" button 650.
  • Control module 140 reattempts the failed command, and, if it completes successfully, continues execution of the procedure.
  • mappings is illustrated in FIG. 7.
  • "Load Recipe” object 705 retrieves a recipe file from memory as discussed above in reference to procedure 405. Instead of simply iterating through each mapping in the recipe file, as described in reference to that procedure, however, procedure 700 allows the user to specify different treatment for specific mappings or groups of mappings.
  • Tag Mapping objects 710-716 cause control module 140 to prompt the user to select one or more mappings for "tagging". For example, "Tag Type 1 Mappings" object 710 prompts the user to identify mappings in the loaded recipe file to be labeled or "tagged” as "Type 1" mappings in subsequent processing steps. Alternatively, mapping tags can be specified in the recipe file loaded by "Load Recipe" object 705.
  • the tag for each Tag Mapping object is specified during creation of the procedure, and can be any combination of characters desired by the procedure's creator.
  • tags can be selected to identify mappings as corresponding to particular types of materials or chemistries, such as, for example, ⁇ e.g., solvents, reagents, monomers, initiators, catalysts, surfactants and the like.
  • procedure 700 carries out the mapping processes specified by "For Each Mapping" object 730. Instead of simply repeating the same processing steps for each mapping in the recipe file, as described for procedure 405 ("For Each Mapping" object 432) above, procedure 700 includes a series of If objects 740-746, which correspond to the mapping tags defined by objects 710-716.
  • each If object 740-746 contains a series of child objects implementing a set of process steps for each mapping identified by the corresponding tag.
  • object 740 and its child objects 750-760 define a series of processing steps for recipe file mappings the user has identified with the "Type 1" tag.
  • "Wash Interval” object 750 and "Air Gap Flag” object 751 are Define Variable objects that cause control module 140 to define a pair of variables that will be available to subsequent code objects in If object 740.
  • "For Each Dispense” object 752 is a For Loop object that iterates through its child objects 753-758 for each dispense of each "Type 1" mapping.
  • "While Liquid to Dispense” object 753 is a While Loop object that iterates through its child objects 754-757 for as long as liquid remains to be dispensed in the current dispense cycle.
  • "Fill Syringe” object 754 is an If object that carries out a series of steps causing the syringe to refill when it becomes empty (using the "Air Gap Flag” variable defined by object 751 to identify when an air gap should be aspirated during the syringe-filling process).
  • "Move to Destination” object 755 causes apparatus 180 to move the specified hardware device to the next destination location
  • "Dispense” object 757 causes apparatus 180 to deposit a specified amount of liquid at that location at a speed set by "Set Syringe Speeds” object 756.
  • "For Each Dispense" object 752 causes control module 140 to iterate to the next dispense in the "Type 1" mapping, causing apparatus 180 to wash the syringe tip according to "Wash On Interval” object 758 (and its child objects) at intervals defined by the "Wash Interval” variable defined by object 750.
  • control module 140 instructs apparatus 180 to wash the syringe tip according to a process defined by "Wash Left Arm” object 759 and its child objects, and to move the arm to a final position ("Park" object 760).
  • objects 741-746 each contain a series of child objects setting out processing steps for implementing dispenses associated with each of the remaining groups of tagged mappings in the recipe file.
  • the library synthesis is complete.
  • the user can create procedures to carry out processes other than library synthesis, such as, for example, screening or characterizing members of a combinatorial library.
  • One such procedure is illustrated in FIG. 8. To implement such processes, system 100 need not obtain library design information from a recipe file.
  • sample Map object 810
  • "Sample Map” object 810 creates one or more mappings to define the library members to be sampled for screening.
  • Control module 140 prompts the user to identify one or more source components for sampling, for example by specifying the identity and location of a multi-well plate defined for system 100.
  • the user specifies a number of samples to be drawn from each well in the designated substrate, for example by entering integer values into a spreadsheet representing a source substrate array.
  • the value of the integer entered into the spreadsheet determines the number of dispenses for the corresponding well.
  • the user defines a destination substrate and position for the mapping, for example an injection port of an analytical device such as a liquid, gas or gel permeation chromatograph.
  • procedure 800 The user completes procedure 800 as described above, for example by defining a "Mapping Loop” object 820, which causes module 140 to iterate through the mappings defined by "Sample Map” object 810. For each dispense in each mapping, procedure 800 causes module 140 to instruct apparatus 180 to dilute and mix the contents of the specified well (If object 830 and child objects 831-838), wash the syringe tip ("Wash” object 840) and wait for a specified interval ("Wait for Timer 1" object 845).
  • "Inject Sample” object 850 contains a series of child objects 851-861 that define the injection process.
  • "Go to source” object 851 causes apparatus 180 140 to move the syringe to the specified source location (e.g., a specified well in a substrate array).
  • "Aspirate” object 852 causes apparatus 180 to draw a sample from the source location.
  • "Go above injector” object 853 causes apparatus 180 to move the syringe to a location above an injection port of the analytical device.
  • "Go into injector” object 855 causes apparatus 180 to move the syringe into the injection port at a speed set by "Set Arm Speeds” object 854 (which is then reset by object 856).
  • "Dispense” object 857 causes module 140 to instruct apparatus 180 to inject the sample into the injection port.
  • "Begin analysis” object 859 causes control module 140 to send a command to the analytical device to begin the analysis.
  • Set Timer objects 860 and 861 then reinitialize Timers 1 and 2 for the next sample.
  • procedure 800 proceeds to the next well (i.e., the next dispense).
  • Control module 140 repeats the procedure until a sample has been drawn from each specified source location and injected into the analytical device.
  • Control module 140 can execute multiple procedures simultaneously. For example, using an automated synthesis robot with two arms, control module 140 can execute a different procedure with each robot arm. However, errors can occur if the procedures attempt to control the same hardware — such as the same arm or syringe. Likewise, any attempt to run simultaneous procedures requiring access to common areas of the work surface can lead to errors unless timer objects are included to synchronize arm movements to avoid collisions.
  • the invention can be implemented in digital electronic circuitry, or in computer hardware, firmware, software, or in combinations of them.
  • Apparatus of the invention can be implemented in a computer program product tangibly embodied in a machine-readable storage device for execution by a programmable processor; and method steps of the invention can be performed by a programmable processor executing a program of instructions to perform functions of the invention by operating on input data and generating output.
  • the invention can be implemented advantageously in one or more computer programs that are executable on a programmable system including at least one programmable processor coupled to receive data and instructions from, and to transmit data and instructions to, a data storage system, at least one input device, and at least one output device.
  • Each computer program can be implemented in a high-level procedural or object-oriented programming language, or in assembly or machine language if desired; and in any case, the language can be a compiled or interpreted language.
  • Suitable processors include, by way of example, both general and special purpose microprocessors. Generally, a processor will receive instructions and data from a read-only memory and/or a random access memory.
  • Storage devices suitable for tangibly embodying computer program instructions and data include all forms of non- volatile memory, including by way of example semiconductor memory devices, such as EPROM, EEPROM, and flash memory devices; magnetic disks such as internal hard disks and removable disks; magneto-optical disks; and CD-ROM disks. Data can also be temporarily stored in volatile memory.
  • the invention can be implemented on a computer system having a display device such as a monitor or LCD screen for displaying information to the user and a keyboard and a pointing device such as a mouse or a trackball by which the user can provide input to the computer system.
  • the computer system can be programmed to provide a graphical user interface through which computer programs interact with users.

Abstract

Methods and apparatus for controlling an automated material handling procedure. The method includes receiving a recipe file comprising component information and mapping information; interacting with a user to create a procedure for executing a set of material handling steps; and causing an automated material-handling apparatus to carry out the set of material-handling steps by executing the procedure. The mapping information relates a source component and a destination component. The mapping information defines one or more transfers of at least one source component material to at least one destination component location. The method includes providing to the user an assortment of pre-programmed code objects and receiving from the user a selection and arrangement of the pre-programmed code objects. The procedure is defined by the user's selection and arrangement. The procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file.

Description

SYNTHESIZING COMBINATORIAL LIBRARIES OF MATERIALS BACKGROUND OF THE INVENTION
The present invention relates to methods and apparatus for controlling automated material-handling procedures.
Traditionally, the discovery and development of materials has predominantly been a trial and error process carried out by scientists who generate data one experiment at a time. This process suffers from low success rates, long time lines and high costs, particularly as the desired materials increase in complexity. When a material is composed of multiple components, theory provides little guidance and the large variety of combinations of components takes a substantial amount of time to prepare and analyze. Combinatorial materials science addresses some of these challenges.
Combinatorial materials science refers generally to methods for creating a collection (or "library") of chemically diverse compounds or materials and/or to methods for rapidly testing or screening such libraries for desirable performance characteristics and properties. By parallel or rapid serial testing of many compounds or materials, combinatorial techniques accelerate the speed of research, facilitate breakthroughs and expand the amount of information available to researchers. Furthermore, the ability to examine the relationships between hundreds or thousands of materials in a short period of time enables scientists to make well-informed decisions in the discovery process and to identify unexpected trends. Implementation of combinatorial screening methods depends on the ability to prepare libraries of materials using combinatorial synthetic techniques. These techniques typically employ a robot to automate library preparation and screening, and computer programs have been used to control such robots.
SUMMARY OF THE INVENTION
The invention features methods and apparatus, including computer program products, operable to control automated material handling procedures. The invention is particularly useful for controlling the automated synthesis and/or screening of combinatorial libraries, but is generally useful for controlling material handling procedures involving the transfer of materials from one or more source locations to one or more destination locations.
In general, in one aspect, the invention features a computer program on a computer-readable medium for controlling a material-handling apparatus. The program includes instructions operable to cause a programmable processor to provide an assortment of pre-programmed code objects; and receive from a user a selection and arrangement of pre-programmed code objects from the assortment. The assortment includes a first pre-programmed code object operable to receive an input defining one or more source components, one or more destination components and one or more mappings. Each mapping relates a source component and a destination component and defines one or more transfers of at least one source component material to at least one destination component locations. The selection defines a set of material handling steps. The arrangement defines an order of execution for the selected pre-programmed code objects.
Implementations of the invention can include one or more of the following advantageous features. The first pre-programmed code object is operable to interact with a user to receive a mapping. The first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations and the destination component with a destination location including an access port of an analytical device. The input is a recipe file including library design information defining the source component, the destination component and the mapping. The material handling steps define a procedure for synthesizing a combinatorial library of materials. The mapping defines a gradient for distributing the source component materials among the destination locations. The first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations, each of which has a source component material, and the destination component with a plurality of destination locations corresponding to a plurality of members of the combinatorial library. The assortment includes at least one second pre-programmed code object operable to label a mapping for processing by at least one third pre-programmed code object to be executed subsequently in the arrangement. The arrangement includes a logical hierarchy including a parent pre-programmed code object and at least one child pre-programmed code object. The parent pre-programmed code object is operable to define a condition determining whether the at least one child pre-programmed code object will be executed subsequently in the arrangement. The parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each mapping defined by the input. The parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each transfer defined by one of the one or more mappings. The condition is a "for" condition. The condition is a "while" condition. The condition is an "if condition. In general, in another aspect, the invention features a computer program for controlling an automated material-handling apparatus for carrying out a set of material-handling steps. The program includes instructions operable to cause a programmable processor to receive a recipe file comprising component information and mapping information; interact with a user to create a procedure for executing the set of material handling steps; and cause the automated material-handling apparatus to carry out the set of material -handling steps by executing the procedure. The mapping information relates a source component and a destination component. The mapping information defines one or more transfers of at least one source component material to at least one destination component location. The program includes instructions to provide to the user an assortment of pre-programmed code objects and receive from the user a selection and arrangement of the pre-programmed code objects. The procedure is defined by the user's selection and arrangement. The procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file. Implementations of the invention can include one or more of the following advantageous features. The program includes instructions operable to cause a programmable processor to interact with a user to create a resource hierarchy including one or more resource objects defining at least one hardware device of the automated material-handling apparatus coupled to the programmable processor. The program includes instructions operable to cause a programmable processor to interact with a user to create a system hierarchy including one or more system objects defining one or more robot arms coupled to the at least one hardware device of the automated material-handling apparatus. The program includes instructions operable to cause a programmable processor to interact with a user to create a substrate hierarchy including one or more substrate objects defining a geometry for at least one substrate. The program includes instructions operable to cause a programmable processor to interact with a user to create a one or more position objects for each substrate object in the substrate hierarchy. At least one substrate object corresponds to one or more source locations. At least one substrate object corresponds to one or more destination locations. Execution of the procedure translates the component information and the mapping information to machine level commands for controlling the automated material-handling apparatus. The program includes instructions operable to enable the user to modify the procedure during execution. In general, in another aspect, the invention features a computer-implemented method for controlling a material-handling apparatus. The method includes providing an assortment of pre-programmed code objects; and receiving from a user a selection and arrangement of pre-programmed code objects from the assortment. The assortment includes a first pre-programmed code object operable to receive an input defining one or more source components, one or more destination components and one or more mappings. Each mapping relates a source component and a destination component and defines one or more transfers of at least one source component material to at least one destination component locations. The selection defines a set of material handling steps. The arrangement defines an order of execution for the selected pre-programmed code objects.
Implementations of the invention can include one or more of the following advantageous features. The first pre-programmed code object is operable to interact with a user to receive a mapping arrangement which defines a material-handling procedure for execution by the material-handling apparatus. The first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations and the destination component with a destination location comprising an access port of an analytical device. The input is a recipe file including library design information defining the source component, the destination component and the mapping. The material handling steps define a procedure for synthesizing a combinatorial library of materials. The mapping defines a gradient for distributing the source component materials among the destination locations. The first pre-programmed code object is operable to interact with a user to associate the source component with one or more source location, each of which has a source component material, and the destination component with a plurality of destination locations corresponding to a plurality of members of the combinatorial library. The assortment includes at least one second pre-programmed code object operable to label a mapping for processing by at least one third pre-programmed code object to be executed subsequently in the arrangement. The arrangement includes a logical hierarchy including a parent pre-programmed code object and at least one child pre-programmed code object. The parent pre-programmed code object is operable to define a condition determining whether the at least one child pre-programmed code object will be executed subsequently in the arrangement. The parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each mapping defined by the input. The parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each transfer defined by one of the one or more mappings. The condition is a "for" condition. The condition is a "while" condition. The condition is an "if condition.
In general, in another aspect, the invention features a method of controlling an automated material-handling apparatus for executing a set of material-dispensing steps. The method includes receiving a recipe file comprising component information and mapping information; interacting with a user to create a procedure for synthesizing the combinatorial library; and causing the automated material-handling apparatus to synthesize the combinatorial library by executing the procedure. The mapping information relates a source component and a destination component. The mapping information defines one or more transfers of at least one source component material to at least one destination component location. The method includes providing to the user an assortment of pre-programmed code objects and receiving from the user a selection and arrangement of the pre-programmed code objects. The procedure is defined by the user's selection and arrangement. The procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file. Implementations of the invention can include one or more of the following advantageous features. The method includes interacting with a user to create a resource hierarchy including one or more resource objects defining at least one hardware device of the automated material-handling apparatus coupled to the programmable processor. The method includes interacting with a user to create a system hierarchy including one or more system objects defining one or more robot arms coupled to the at least one hardware device of the automated material-handling apparatus. The method includes interacting with a user to create a substrate hierarchy including one or more substrate objects defining a geometry for at least one substrate. The method includes interacting with a user to create a one or more position objects for each substrate object in the substrate hierarchy. At least one substrate object corresponds to one or more source locations. At least one substrate object corresponds to one or more destination locations. Execution of the procedure translates the component information and the mapping information to machine level commands for controlling the automated material-handling apparatus. The method includes modifying the procedure during execution.
In general, in another aspect, the invention features a system for executing a set of material-handling steps. The system includes means for receiving a recipe file comprising component information and mapping information; means for interacting with a user to create a procedure for synthesizing a combinatorial library of materials; and means for synthesizing the combinatorial library by executing the procedure. The mapping information relates a source component and a destination component and defining one or more transfers of at least one source component material to at least one destination component location. The means for interacting with the user includes means for providing to the user an assortment of pre-programmed code objects and means for receiving from the user a selection and arrangement of the pre-programmed code objects. The procedure is defined by the user's selection and arrangement. The procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file.
Advantages that can be seen in implementations of the invention include one or more of the following. The use of code blocks allows users to create highly customized procedures for a wide variety of material -handling processes including preparation or synthesis of combinatorial libraries as well as characterization of libraries of materials. New code blocks can be generated and collections created storing code blocks for use in future procedures. Procedures can be changed on the fly to allow experimentation and process exploration. Procedures are not restricted to specific hardware. The use of code blocks in a graphical programming environment allows users to create procedures without requiring knowledge of complicated programming languages. Procedures can be run with minimal user interaction during execution. The use of high level library design data allows the synthesis of libraries designed in different design environments.
The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below. Other features and advantages of the invention will become apparent from the description, the drawings, and the claims.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a block diagram illustrating a system for controlling an automated material handling procedure.
FIG. 2 is a flow diagram generally illustrating a method of controlling an automated material handling procedure. FIG. 3 is a flow diagram illustrating a method of controlling an automated material handling procedure in more detail.
FIG. 4 is an example of a dialog window displaying a procedure generated according to the claimed invention.
FIG. 5 is a flow diagram illustrating a method of generating a procedure. FIG. 6 is an example of a dialog window displaying a list of procedures and an event log according to the claimed invention.
FIG. 7 is a window illustrating a procedure for processing multiple tagged mappings.
FIG. 8 is a window illustrating a material-handling procedure for characterizing a library of materials.
Like reference numbers and designations in the various drawings indicate like elements.
DETAILED DESCRIPTION A series of user-selected procedures is used to implement a set of material- processing instructions. In one embodiment that will be described, the instructions are obtained from a recipe file for the automated synthesis of a combinatorial library of materials. Materials can include any type of chemical compound, mixture or other material of interest. The combinatorial library of materials is prepared by placing components in a destination, such as a microtiter plate, silicon wafer or the like. Each destination includes an array of regions, which may include, for example, the wells of a microtiter plate or locations on a wafer. The resulting set of materials is the library, which includes a set of "members" corresponding to materials at each region of the destination array. A library can be arranged in any convenient geometric shape, such as a square, rectangle, circle or triangle. For discussions of combinatorial strategies, library design strategies and a related discussion of regions, see U.S. Application Serial No. 09/156,827, filed September 18, 1998, and U.S. Application Serial No. 09/174,856, filed on October 19, 1998, and U.S. Patent No. 5,776,359, all of which are incorporated herein by reference in their entireties. As shown in FIGS. 1 and 2, a system 100 includes a computer 110 equipped with input/output devices 120. A set of material handling instructions is generated in design module 130 and provided to control module 140 (step 200). Alternatively, control module 140 retrieves instructions from memory 150. Suitable instructions can be provided in a
"Recipe file" and can include, for example, component information identifying source and destination components (representing physical containers such as vials or microtiter plates or physical locations such as regions on a substrate wafer) to be used in the synthesis of a library, mapping information relating each source to one or more destination components and identifying amounts of each source to be used to synthesize each library member, and parameters defining reaction conditions for the combination of components to form individual library members.
Mapping information can include, for example, a list of specific amounts of materials to be transferred from one or more source vials to each of a set of destination wells. Mapping information can also include gradients specifying a range of amounts of a material or materials to be transferred from a particular source to a series of wells, with the amount to be transferred to each destination well determined according to a linear, exponential, logarithmic, polynomial, geometric or other mathematical expression. The creation of recipe files is described in co-pending U.S. Application Serial No. 09/174,856, filed on October 19, 1998, which is incorporated herein by reference in its entirety.
Control module 140 captures the component, mapping and parameter information from a recipe file and makes the information available for use by user-defined procedures as will be described below.
A typical user of system 100 is a chemist. Through user interface 160, the user initializes system 100 and creates a procedure using object editor 170 (step 210). Control module 140 executes the procedure (step 220), causing material handling apparatus 180 to, for example, synthesize a combinatorial library specified by the set of instructions in the recipe file. In the described embodiment, control module 140 communicates directly with apparatus 180, for example, by transmitting text commands through a serial port or ports to apparatus 180. Alternatively, commands generated by module 140 can be implemented by drivers for apparatus 180 installed on computer 110.
Appropriate apparatus 180 can include, for example, automated liquid handling robots, such as the RSP 900 Robotic Sample Processor, available from Cavro Scientific Instruments, Inc. of Sunnyvale, California, or equivalents. Apparatus 180 can also include automated systems performing different types of physical or chemical vapor deposition. In liquid handling, mixtures of solutions are typically dispensed in an array of miniature wells to create a library. In vacuum deposition, solid elements or chemicals or mixtures of solid elements or chemicals are vaporized and deposited as individual components on a substrate. The deposition may be controlled by a series of shutters and masks to manufacture the library. For an example of deposition equipment, see WO98/47613 (U.S. Application Serial No. 08/841 ,423), filed on April 22, 1997.
Referring to FIG. 3, a method of using system 100 to synthesize a combinatorial library of materials is described in more detail. In each of the first four steps of the method (steps 300, 310, 320 and 330), the user employs object editor 170 to create and configure data objects defining the hardware and processes for the desired synthesis. Each data object has an associated set of properties. The user selects and inserts data objects into a hierarchy of objects defining the hardware and processes for the system, and customizes the selected objects by manipulating the properties associated with each object as will be described in more detail below. Data objects are generated by the editor 170 through the use of computer programming techniques conventional in object-oriented programming. The editor is advantageously implemented to have a graphical user interface.
In one embodiment, as shown in FIG. 4, user interface 160 displays window 400 including an object hierarchy or tree 403 (in this case for procedure objects 405 and 408) in pane 410. The user manipulates the properties of selected objects — for example, properties 414 and 418 of selected For Loop object 432 — by entering text or selecting values in the corresponding fields of pane 420, in this example, fields 424 and 428. The user need not have any familiarity with the generation of the data objects themselves, or of the object-oriented programming techniques used to generate the data objects, in order to manipulate data objects as described below.
Referring again to FIG. 3, the user defines the physical hardware resources available to system 100 through user interface 160 (step 300). As described above, appropriate hardware resources includes devices such as automated synthesis robots, syringe pump controllers, physical and vapor deposition systems and the like. Hardware resources can also include temperature controllers for regulating the temperature of one or more source or destination vials or plates, syringes or other dispensing apparatus. The user defines each hardware device attached to system 100 by creating a hardware resource data object specifically configured for the device in question, for example, specifying a serial port through which the device is attached to computer 110. Each hardware resource object implements a protocol for communication between computer 110 and the particular device in question, giving computer 110 (and the user) access to the device's functionality.
The user defines the properties of the specific arms, syringes, shutters, masks or other devices installed in system 100 by creating system data objects for each such device (step 310). Each system object identifies a hardware resource selected from those already defined (e.g., a particular robot or pump controller) that will control the device in question. Other system object properties include the device address, the configuration of syringes attached to an arm (e.g., in series or in parallel), motion limits, step size and reference positions for arm movements, and resolution, volume and dispensing characteristics for syringes and the like.
The user also identifies each source of starting materials and each destination by creating a substrate data object defining the geometry for each single-well or multi-well substrate to be accessed by system 100; specific positions for these substrates on the work surface are defined using position objects, which the user inserts as child objects of the respective substrate object (step 320). For a substrate object corresponding to a single well substrate or vial, user-defined properties include, for example, the vial's cross-sectional area and height, as well as the position at which the system will dispense liquid into the vial. A vial position object associated with each vial defines the coordinates of the vial's location on the work surface. Properties for two-dimensional substrate arrays include, for example, all of those associated with the single well substrate, as well as the number of rows and columns and the relevant spacing. A plate position object defines the location of the two-dimensional array on the work surface by specifying, for example, grid coordinates of opposite corners of the array.
The user creates a procedure object (step 330) by assembling a hierarchy of code objects that can be executed by control module 140 and robot 180. The process of using object editor 170 to create a liquid-dispensing procedure is described in reference to FIG. 5. The user creates a procedure object using object editor 170 (step 500). The user selects a code object for performing a desired function — such as loading synthesis instructions, initializing one or more robot arms, setting parameters for arm and syringe movement, moving arms, aspirating or dispensing liquids with syringes and the like — from a set of objects accessible by object editor 170 and inserts the object at the appropriate location in the procedure object's logical hierarchy (step 510). The user can also select a "container" object from a code library. As suggested by the name, container objects are containers used to group other objects into functional units, which can then be saved, copied and inserted into other procedures as a unit. Container objects can include conditional "For Loop", "If and "While Loop" objects including one or more functional descendant or child objects that are executed zero, one or more times in a procedure according to the occurrence of a specified condition, as is described in more detail below.
An example of a selection of suitable code objects is provided in Table 1, below. Table 1
Figure imgf000013_0001
Figure imgf000014_0001
Figure imgf000015_0001
Object Name Properties Description
For Loop Loop Type Mapping, Dispense or Numeric. If Mapping, object iterates through mappings loaded on system; if Dispense, object iterates through each dispense within current mapping; if Numeric, object iterates through specified integer range.
Mappings to All or selected (Tagged) mappings included in Include iteration
Mapping Tag Specifies tag to match when iterating through mappings; only mappings with specified tag included in loop
Lookahead Constant or Variable Interval Type
Lookahead Number of wells to consider when calculating Interval mapping aspirate amount or variable name
Loop Variable Name of variable containing numeric loop's current value
Initial Value Constant or Variable Type
Initial Value Initial Value or variable name
Final Value Constant or Variable Type
Final Value Final Value or variable name
Get Controller Temperature controller to use, selected from Temperature controllers defined for system
Channel Type Constant or Variable
Channel Channel number or variable name
Temperature Name of variable into which retrieved Variable temperature value should be put
Get Timer Timer ID ID of timer to be queried
Figure imgf000017_0001
Object Name Properties Description
Move Arm Arm Arm to move, selected from arms defined for system
Wait For Wait for command to complete before continuing Completion (Yes or No)
Destination Mapping Source, Mapping Destination, Substrate, Absolute Position or Relative Position to which arm is to move
Z Position Z position to move to when destination is a substrate well
Substrate Destination substrate, selected from list of substrates defined for system
Position Position of destination substrate on work surface, selected from list of positions defined for selected substrate
Well Specifier Row and column numbers or well number of substrate well
Row Type Constant or Variable
Row Destination well's row number or variable name
Column Type Constant or Variable
Column Destination well's column number or variable name
Well Number Constant or Variable. Type
Well Number Destination well's well number or variable name
Absolute X, Y X, Y and Z coordinates of the destination & Z position
Relative X, Y Relative amounts to move arm in X, Y and Z & Z
Pause Prompt Text Text displayed in pause dialog
Figure imgf000019_0001
Figure imgf000020_0001
Figure imgf000021_0001
Figure imgf000022_0001
Figure imgf000023_0001
Figure imgf000024_0001
The user defines the properties associated with the chosen code object (step 520). If additional code objects are required to complete the procedure (the YES branch of step 30), the user selects the next desired code object (step 510) and repeats the process.
When the last code object has been selected and defined (the NO branch of step 530), the procedure is complete. At this point, or at any time during creation of the procedure, the user can validate the code to verify the consistency of the procedure, or any selected code object and its descendants (step 540). Object editor 170 stores the procedure in memory 150 and/or provides the procedure to control module 140 for execution by apparatus 180 (step 550).
A sample procedure generated according to the steps discussed above is illustrated in FIG. 4. Procedure object 405, which the user has named "Simple Mapping w/ Left Arm", includes tree 403 of code objects 430-451. Each code object in tree 403 is represented by an icon, for example icon 460, which corresponds to the object type, and by a name, which can be a default name based on the object type or a name chosen by the user. Procedure 405 includes a series of code objects that, when executed, cause control module 140 to instruct apparatus 180 to carry out a simple mapping procedure with the left arm of a synthesis robot. Control module 140 executes procedure 405 as follows. "Initialize Arm" object 430 initializes an arm of the robot, setting the arm and syringe speeds and the syringe maximum aspirate and minimum dispense volumes to default values. "Load Recipe" object 431 loads component, mapping and parameter information from a specified recipe file. As described above, a suitable recipe file contains component information identifying the source and destination components to used during execution of the procedure, as well as mapping information specifying amounts to be transferred from each source component to one or more destination components. The recipe file can also contain parameter information, in the form of values or sets of values associated with components identified in the recipe file.
Execution of "Load Recipe" object 431 causes control module 140 to prompt the user for the name of a recipe file to load. Optionally, a specific recipe file can be identified as a property of "Load Recipe" object 431. Control module 140 loads the specified recipe file and causes user interface 160 to display tables of component and mapping information. Control module 140 prompts the user to identify and provide a position on the work surface for each substrate component defined in step 320, discussed above. A given substrate can be used as a source (e.g., a location from which a reagent is transferred), a destination (a location to which a reagent is transferred), or both (e.g., a location in which two or more reagents are mixed and the mixture transferred to another location) during execution of a procedure. Any parameter data in the recipe file is associated with the specified substrate objects, and is made available to subsequent code objects in procedure 405. Thus, for example, if the recipe file loaded by object 431 contained "Temperature" parameter information including a value for each well in a specified destination plate, those values would be available to subsequent code objects invoking a predefined "Temperature" variable defined using a "Define Variable" object as set out in Table 1 and discussed in more detail below. A set of "Temperature" parameters can be advantageously invoked by "Set Temperature", "Get Temperature" and "Wait for Temperature" objects as set out in Table 1.
"For Each Mapping" object 432 is a container object — specifically a For Loop object ~ that executes its set of child objects for a specified number of iterations. In this case, object 432 is a "mapping loop" that iterates over each mapping in the recipe file loaded by "Load Recipe" object 431. Object 432 causes control module 140 to define a "Total Mappings" variable that contains the total number of mappings loaded from the recipe file, and a "Mapping Number" variable that is set to the index of the current mapping and ranges from one to the value of Total Mappings. Both of these variables are available to all child objects of loop object 432. The child objects of loop 432 are objects 433-450, which are executed by control module 140 for each loaded mapping. "For Each Dispense" object 433 is another For Loop object, this time iterating through each dispense specified in the current mapping (corresponding to the mapping identified by the Mapping Number variable discussed above). Object 433 causes control module 140 to define eight system variables available to all child objects of loop object 433. These include a "Total Dispenses" variable containing the number of dispenses for the current mapping and a "Dispense Number" variable containing the index of the current dispense and ranging from one to the value of "Total Dispenses". These variables also include a "Mapping Aspirate Volume" variable containing the volume to aspirate (or retrieve from a source component), based on the mapping destination well (or on multiple wells based on a predetermined "look ahead interval" specifying a set number of wells to be considered when calculating the amount to aspirate) and a "Mapping Dispense Volume" variable containing the volume to dispense for the current mapping destination; for each dispense, both of these variables are initialized to preset values for the dispense and are updated as the dispense progresses. Finally, object 433 also causes control module 140 to define "Source Row" and "Source Column" variables indicating the row and column of the current source well and "Destination Row" and "Destination Column" variables indicating the row and column of the current destination well. The child objects for loop object 433 are objects 434-441, which are repeated in sequence for each dispense in each loaded mapping.
"While Liquid to Dispense" object 434 is another type of container object ~ a While Loop object — that causes all child objects to be executed repeatedly as long as a specified condition is true. In this case, object 434 causes control module 140 to determine whether the value of the Mapping Dispense Volume variable is greater than zero, indicating that the dispense still calls for liquid to be dispensed. For as long as this expression evaluates to true, control module 140 executes child objects 435-441. When the expression evaluates to false (i.e., no volume remains to be dispensed), the current dispense is complete and control module 140 proceeds to the next iteration of "For Each Dispense" loop 433. "While" object 434 can implement any convenient logical expression, such as arithmetic expressions comparing one value with another value. While Loop object 434 can also implement "syringe conditions" that evaluate whether a given syringe needs to be refilled, or "mapping tag" conditions that evaluate whether the current mapping has been identified for particular action by the user. "Fill Syringe" object 435 is still another container object ~ an If object — that causes control module 140 to execute its child objects if a specified condition is true. In this case, object 435 causes control module 140 to determine whether the specified syringe must be refilled (i.e., when a "syringe empty" condition evaluates to "true"). Here, when the syringe is empty, object 435 causes control module 140 to execute "Move to Source" object 436, a Move Arm object that instructs apparatus 180 to move the syringe to the location of the specified source component, and Aspirate object 437, which instructs the syringe to aspirate from that source component an amount determined by the "Mapping Aspirate Volume" variable defined for the current dispense.
When the syringe is not empty, "Move to Destination" object 438 (another Move Arm object) causes apparatus 180 to move the loaded syringe to the location of the specified destination component. "Dispense" object 439 causes apparatus 180 to dispense into the specified destination well an amount determined by the Mapping Dispense Volume variable defined for the current dispense. "Set Timer" and "Wait For Timer" objects 440 and 441 cause control module 140 to pause for a preset (or variable) period of time before proceeding to the next dispense iteration. After completing all dispenses for the current mapping (and after each successive mapping), control module 140 executes Container object 442, named "Standard Wash - Left 500ul". "Move To Waste" object 443, another Move Arm object, causes apparatus 180 to move the syringe to the location of a "waste" destination. "Purge Syringe" object 444 causes apparatus 180 to empty all contents of the specified syringe into the waste destination. "Dispense" object 445 causes apparatus 180 to refill the specified syringe with a specified volume from a reservoir (typically filled with a solvent such as, for example, water) and dispense the specified volume. Control module 140 causes apparatus 180 to move the specified syringe to a cleaning station ("Move To Clean 1" object 446), where the syringe is again filled with solvent, which is dispensed (Dispense object 447). "Move To Clean 2" object 448 causes apparatus 180 to move the specified syringe to a second cleaning station, for a final wash ("Dispense" object 449). "Move Home" object
450 causes apparatus 180 to move the specified arm back to a home position, from which the next mapping iteration begins. When all mappings have been processed, "Park" object
451 (a final Move Arm object) causes apparatus 180 to return the arm to its home position.
FIG. 6 shows a user interface window 600. The user instructs control module 140 to execute a completed procedure, for example by activating "Execute" button 620 in status pane 610 of window 600, which causes user interface 160 to display a list of available procedures in computer 110. Status pane 610 includes a line item 630 indicating that control module 140 is executing a procedure. While a procedure executes, user interface 160 displays status messages in event log 640. The user can pause or abort a procedure during execution by selecting the appropriate line item and activating button 650 or 660, respectively.
Depending on the user's preference, event log 640 can provide a record of high level events, or it can provide a detailed record of each step in a procedure. Event log 640 also displays any errors occurring during execution. When an error occurs, control module 140 pauses the executing procedure and posts the error to event log 640. While the procedure is paused, the user can execute other procedures in order to correct the error. For example, if the error resulted from a mistrained substrate position, the user can attempt to retrain the position. The user can also modify the paused procedure itself using object editor 170. When the error has been corrected, the user instructs control module
140 to resume execution of the original procedure by again activating "pause" button 650. Control module 140 reattempts the failed command, and, if it completes successfully, continues execution of the procedure.
Using the techniques described above, the user can customize procedures for a wide variety of automated processes. One such procedure 700, named "Tagged
Mappings", is illustrated in FIG. 7. "Load Recipe" object 705 retrieves a recipe file from memory as discussed above in reference to procedure 405. Instead of simply iterating through each mapping in the recipe file, as described in reference to that procedure, however, procedure 700 allows the user to specify different treatment for specific mappings or groups of mappings. Tag Mapping objects 710-716 cause control module 140 to prompt the user to select one or more mappings for "tagging". For example, "Tag Type 1 Mappings" object 710 prompts the user to identify mappings in the loaded recipe file to be labeled or "tagged" as "Type 1" mappings in subsequent processing steps. Alternatively, mapping tags can be specified in the recipe file loaded by "Load Recipe" object 705. The tag for each Tag Mapping object is specified during creation of the procedure, and can be any combination of characters desired by the procedure's creator. Thus, tags can be selected to identify mappings as corresponding to particular types of materials or chemistries, such as, for example, ~ e.g., solvents, reagents, monomers, initiators, catalysts, surfactants and the like. After the robot arms are initialized by objects 720 and 725, procedure 700 carries out the mapping processes specified by "For Each Mapping" object 730. Instead of simply repeating the same processing steps for each mapping in the recipe file, as described for procedure 405 ("For Each Mapping" object 432) above, procedure 700 includes a series of If objects 740-746, which correspond to the mapping tags defined by objects 710-716. As shown for "If Type 1" object 740, each If object 740-746 contains a series of child objects implementing a set of process steps for each mapping identified by the corresponding tag. Thus, "If Type 1 "object 740 and its child objects 750-760 define a series of processing steps for recipe file mappings the user has identified with the "Type 1" tag.
"Wash Interval" object 750 and "Air Gap Flag" object 751 are Define Variable objects that cause control module 140 to define a pair of variables that will be available to subsequent code objects in If object 740. "For Each Dispense" object 752 is a For Loop object that iterates through its child objects 753-758 for each dispense of each "Type 1" mapping. "While Liquid to Dispense" object 753 is a While Loop object that iterates through its child objects 754-757 for as long as liquid remains to be dispensed in the current dispense cycle. "Fill Syringe" object 754 is an If object that carries out a series of steps causing the syringe to refill when it becomes empty (using the "Air Gap Flag" variable defined by object 751 to identify when an air gap should be aspirated during the syringe-filling process). "Move to Destination" object 755 causes apparatus 180 to move the specified hardware device to the next destination location, and "Dispense" object 757 causes apparatus 180 to deposit a specified amount of liquid at that location at a speed set by "Set Syringe Speeds" object 756. When the dispense is complete, "For Each Dispense" object 752 causes control module 140 to iterate to the next dispense in the "Type 1" mapping, causing apparatus 180 to wash the syringe tip according to "Wash On Interval" object 758 (and its child objects) at intervals defined by the "Wash Interval" variable defined by object 750. When all dispenses in the "Type 1" mapping have been processed, control module 140 instructs apparatus 180 to wash the syringe tip according to a process defined by "Wash Left Arm" object 759 and its child objects, and to move the arm to a final position ("Park" object 760).
In a similar fashion, If objects 741-746 each contain a series of child objects setting out processing steps for implementing dispenses associated with each of the remaining groups of tagged mappings in the recipe file. When each tagged mapping has been processed according to the appropriate If object and its child objects, the library synthesis is complete. Depending on the resources, system devices and substrates defined for system 100, the user can create procedures to carry out processes other than library synthesis, such as, for example, screening or characterizing members of a combinatorial library. One such procedure is illustrated in FIG. 8. To implement such processes, system 100 need not obtain library design information from a recipe file. Instead, to define a screening process the user creates a procedure 800 as described above but incorporating "Sample Map" object 810 in place of the "Load Recipe" object discussed above. When executed by control module 140, "Sample Map" object 810 creates one or more mappings to define the library members to be sampled for screening. Control module 140 prompts the user to identify one or more source components for sampling, for example by specifying the identity and location of a multi-well plate defined for system 100. The user specifies a number of samples to be drawn from each well in the designated substrate, for example by entering integer values into a spreadsheet representing a source substrate array. The value of the integer entered into the spreadsheet determines the number of dispenses for the corresponding well. The user defines a destination substrate and position for the mapping, for example an injection port of an analytical device such as a liquid, gas or gel permeation chromatograph.
The user completes procedure 800 as described above, for example by defining a "Mapping Loop" object 820, which causes module 140 to iterate through the mappings defined by "Sample Map" object 810. For each dispense in each mapping, procedure 800 causes module 140 to instruct apparatus 180 to dilute and mix the contents of the specified well (If object 830 and child objects 831-838), wash the syringe tip ("Wash" object 840) and wait for a specified interval ("Wait for Timer 1" object 845).
"Inject Sample" object 850 contains a series of child objects 851-861 that define the injection process. "Go to source" object 851 causes apparatus 180 140 to move the syringe to the specified source location (e.g., a specified well in a substrate array). "Aspirate" object 852 causes apparatus 180 to draw a sample from the source location. "Go above injector" object 853 causes apparatus 180 to move the syringe to a location above an injection port of the analytical device. "Go into injector" object 855 causes apparatus 180 to move the syringe into the injection port at a speed set by "Set Arm Speeds" object 854 (which is then reset by object 856). "Dispense" object 857 causes module 140 to instruct apparatus 180 to inject the sample into the injection port. After waiting for a preset interval (as specified by "Wait for Timer 2" object 858), "Begin analysis" object 859 causes control module 140 to send a command to the analytical device to begin the analysis. Set Timer objects 860 and 861 then reinitialize Timers 1 and 2 for the next sample. After washing the syringe tip ("Wash" object 862), procedure 800 proceeds to the next well (i.e., the next dispense). Control module 140 repeats the procedure until a sample has been drawn from each specified source location and injected into the analytical device.
Control module 140 can execute multiple procedures simultaneously. For example, using an automated synthesis robot with two arms, control module 140 can execute a different procedure with each robot arm. However, errors can occur if the procedures attempt to control the same hardware — such as the same arm or syringe. Likewise, any attempt to run simultaneous procedures requiring access to common areas of the work surface can lead to errors unless timer objects are included to synchronize arm movements to avoid collisions. The invention can be implemented in digital electronic circuitry, or in computer hardware, firmware, software, or in combinations of them. Apparatus of the invention can be implemented in a computer program product tangibly embodied in a machine-readable storage device for execution by a programmable processor; and method steps of the invention can be performed by a programmable processor executing a program of instructions to perform functions of the invention by operating on input data and generating output. The invention can be implemented advantageously in one or more computer programs that are executable on a programmable system including at least one programmable processor coupled to receive data and instructions from, and to transmit data and instructions to, a data storage system, at least one input device, and at least one output device. Each computer program can be implemented in a high-level procedural or object-oriented programming language, or in assembly or machine language if desired; and in any case, the language can be a compiled or interpreted language. Suitable processors include, by way of example, both general and special purpose microprocessors. Generally, a processor will receive instructions and data from a read-only memory and/or a random access memory. Storage devices suitable for tangibly embodying computer program instructions and data include all forms of non- volatile memory, including by way of example semiconductor memory devices, such as EPROM, EEPROM, and flash memory devices; magnetic disks such as internal hard disks and removable disks; magneto-optical disks; and CD-ROM disks. Data can also be temporarily stored in volatile memory. Any of the foregoing can be supplemented by, or incorporated in, ASICs (application-specific integrated circuits). To provide for interaction with a user, the invention can be implemented on a computer system having a display device such as a monitor or LCD screen for displaying information to the user and a keyboard and a pointing device such as a mouse or a trackball by which the user can provide input to the computer system. The computer system can be programmed to provide a graphical user interface through which computer programs interact with users.
The invention has been described in terms of particular embodiments. Other embodiments are within the scope of the following claims. For example, the steps of the invention can be performed in a different order and still achieve desirable results. While the invention has been described in the context of a liquid handling system and procedure, the invention is equally applicable to other synthesis systems and techniques, such as vapor deposition systems as discussed above. Likewise, while the invention has been described in the context of specific hardware resources, system devices, substrates and code objects, additional data objects can be defined for other types of hardware resources, devices, substrates and process steps.

Claims

What is claimed is:
1. A computer program on a computer-readable medium for controlling a material- handling apparatus, the program comprising instructions operable to cause a programmable processor to: provide an assortment of pre-programmed code objects, the assortment comprising a first pre-programmed code object operable to receive an input defining one or more source components, one or more destination components and one or more mappings, each mapping relating a source component and a destination component and defining one or more transfers of at least one source component material to at least one destination component locations; and receive from a user a selection and arrangement of pre-programmed code objects from the assortment, the selection defining a set of material handling steps and the arrangement defining an order of execution for the selected pre-programmed code objects.
2. The computer program of claim 1 , wherein the first pre-programmed code object is operable to interact with a user to receive a mapping.
3. The computer program of claim 2, wherein the first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations and the destination component with a destination location comprising an access port of an analytical device.
4. The computer program of claim 1 , wherein the input is a recipe file including library design information defining the source component, the destination component and the mapping, and wherein the material handling steps define a procedure for synthesizing a combinatorial library of materials.
5. The computer program of claim 4, wherein the mapping defines a gradient for distributing the source component materials among the destination locations.
6. The computer program of claim 4, wherein the first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations, each source location having a source component material, and the destination component with a plurality of destination locations corresponding to a plurality of members of the combinatorial library.
7. The computer program of claim 1 , wherein the assortment includes at least one second pre-programmed code object operable to label a mapping for processing by at least one third pre-programmed code object to be executed subsequently in the arrangement.
8. The computer program of claim 1 , wherein the arrangement comprises a logical hierarchy including a parent pre-programmed code object and at least one child preprogrammed code object.
9. The computer program of claim 8, wherein the parent pre-programmed code object is operable to define a condition determining whether the at least one child preprogrammed code object will be executed subsequently in the arrangement.
10. The computer program of claim 9, wherein the parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each mapping defined by the input.
11. The computer program of claim 9, wherein the parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each transfer defined by one of the one or more mappings.
12. The computer program of claim 9, wherein the condition is a "for" condition.
13. The computer program of claim 9, wherein the condition is a "while" condition.
14. The computer program of claim 9, wherein the condition is an "if condition.
15. For use in a system including an automated material-handling apparatus for carrying out a set of material-handling steps, a computer program on a computer-readable medium for controlling the automated material-handling apparatus, the program comprising instructions operable to cause a programmable processor to: receive a recipe file comprising component information and mapping information, the mapping information relating a source component and a destination component and defining one or more transfers of at least one source component material to at least one destination component location; interact with a user to create a procedure for executing the set of material handling steps, including instructions to provide to the user an assortment of pre-programmed code objects and receive from the user a selection and arrangement of the pre-programmed code objects, the procedure being defined by the user's selection and arrangement; and cause the automated material-handling apparatus to carry out the set of material- handling steps by executing the procedure, wherein the procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file.
16. The computer program of claim 15, further comprising instructions operable to cause a programmable processor to: interact with a user to create a resource hierarchy including one or more resource objects defining at least one hardware device of the automated material-handling apparatus coupled to the programmable processor.
17. The computer program of claim 16, further comprising instructions operable to cause a programmable processor to: interact with a user to create a system hierarchy including one or more system objects defining one or more robot arms coupled to the at least one hardware device of the automated material-handling apparatus.
18. The computer program of claim 16, further comprising instructions operable to cause a programmable processor to: interact with a user to create a substrate hierarchy including one or more substrate objects defining a geometry for at least one substrate.
19. The computer program of claim 18, further comprising instructions operable to cause a programmable processor to: interact with a user to create a one or more position objects for each substrate object in the substrate hierarchy.
20. The computer program of claim 18, wherein at least one substrate object corresponds to one or more source locations.
21. The computer program of claim 18, wherein at least one substrate object corresponds to one or more destination locations.
22. The computer program of claim 15, wherein: execution of the procedure translates the component information and the mapping information to machine level commands for controlling the automated material-handling apparatus.
23. The computer program of claim 15, further comprising instructions operable to enable the user to modify the procedure during execution.
24. A computer-implemented method for controlling a material -handling apparatus, the method comprising: providing an assortment of pre-programmed code objects, the assortment comprising a first pre-programmed code object operable to receive an input defining one or more source components, one or more destination components and one or more mappings, each mapping relating a source component and a destination component and defining one or more transfers of at least one source component material to at least one destination component locations; and receiving from a user a selection and arrangement of pre-programmed code objects from the assortment, the selection defining a set of material handling steps and the arrangement defining an order of execution for the selected pre-programmed code objects.
25. The method of claim 24, wherein the first pre-programmed code object is operable to interact with a user to receive a mapping arrangement defines a material-handling procedure for execution by the material-handling apparatus.
26. The method of claim 25, wherein the first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations and the destination component with a destination location comprising an access port of an analytical device.
27. The method of claim 24, wherein the input is a recipe file including library design information defining the source component, the destination component and the mapping, and wherein the material handling steps define a procedure for synthesizing a combinatorial library of materials.
28. The method of claim 27, wherein the mapping defines a gradient for distributing the source component materials among the destination locations.
29. The method of claim 27, wherein the first pre-programmed code object is operable to interact with a user to associate the source component with one or more source locations, each source location having a source component material, and the destination component with a plurality of destination locations corresponding to a plurality of members of the combinatorial library.
30. The method of claim 24, wherein the assortment includes at least one second pre-programmed code object operable to label a mapping for processing by at least one third pre-programmed code object to be executed subsequently in the arrangement.
31. The method of claim 24, wherein the arrangement comprises a logical hierarchy including a parent pre-programmed code object and at least one child pre-programmed code object.
32. The method of claim 31 , wherein the parent pre-programmed code object is operable to define a condition determining whether the at least one child pre-programmed code object will be executed subsequently in the arrangement.
33. The method of claim 32, wherein the parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each mapping defined by the input.
34. The method of claim 32, wherein the parent pre-programmed code object is operable to provide that the at least one child pre-programmed code object will be executed at least one time for each transfer defined by one of the one or more mappings.
35. The method of claim 32, wherein the condition is a "for" condition.
36. The method of claim 32, wherein the condition is a "while" condition.
37. The method of claim 32, wherein the condition is an "if condition.
38. A method of controlling an automated material -handling apparatus for executing a set of material-dispensing steps, the method comprising: receiving a recipe file comprising component information and mapping information, the mapping information relating a source component and a destination component and defining one or more transfers of at least one source component material to at least one destination component location; interacting with a user to create a procedure for synthesizing the combinatorial library, comprising providing to the user an assortment of pre-programmed code objects and receiving from the user a selection and arrangement of the pre-programmed code objects, the procedure being defined by the user's selection and arrangement; and causing the automated material-handling apparatus to synthesize the combinatorial library by executing the procedure, wherein the procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file.
39. The method of claim 38, further comprising: interacting with a user to create a resource hierarchy including one or more resource objects defining at least one hardware device of the automated material -handling apparatus coupled to the programmable processor.
40. The method of claim 39, further comprising: interacting with a user to create a system hierarchy including one or more system objects defining one or more robot arms coupled to the at least one hardware device of the automated material-handling apparatus.
41. The method of claim 39, further comprising: interacting with a user to create a substrate hierarchy including one or more substrate objects defining a geometry for at least one substrate.
42. The method of claim 41 , further comprising: interacting with a user to create a one or more position objects for each substrate object in the substrate hierarchy.
43. The method of claim 41 , wherein at least one substrate object corresponds to one or more source locations.
44. The method of claim 41, wherein at least one substrate object corresponds to one or more destination locations.
45. The method of claim 38, wherein: execution of the procedure translates the component information and the mapping information to machine level commands for controlling the automated material-handling apparatus.
46. The method of claim 38, further comprising modifying the procedure during execution.
47. A system for executing a set of material-handling steps, comprising: means for receiving a recipe file comprising component information and mapping information, the mapping information relating a source component and a destination component and defining one or more transfers of at least one source component material to at least one destination component location; means for interacting with a user to create a procedure for synthesizing a combinatorial library of materials, comprising means for providing to the user an assortment of pre-programmed code objects and means for receiving from the user a selection and arrangement of the pre-programmed code objects, the procedure being defined by the user's selection and arrangement; and means for synthesizing the combinatorial library by executing the procedure, wherein the procedure contains at least one first pre-programmed code object operable to read and interpret the recipe file and at least one second pre-programmed code object operable to interpret a mapping read from the recipe file.
PCT/US2000/012228 1999-05-05 2000-05-05 Synthesizing combinatorial libraries of materials WO2000067086A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DK00928837T DK1175645T3 (en) 1999-05-05 2000-05-05 Synthesizing combinatorial material libraries
EP00928837A EP1175645B1 (en) 1999-05-05 2000-05-05 Synthesizing combinatorial libraries of materials
AT00928837T ATE245286T1 (en) 1999-05-05 2000-05-05 COMBINATORIAL GENERATION OF MATERIAL LIBRARIES
DE60003913T DE60003913T2 (en) 1999-05-05 2000-05-05 COMBINATIVE GENERATION OF MATERIAL LIBRARIES
JP2000615862A JP2002542943A (en) 1999-05-05 2000-05-05 Integration of combinatorial libraries of materials
CA002370573A CA2370573A1 (en) 1999-05-05 2000-05-05 Synthesizing combinatorial libraries of materials
AU47013/00A AU4701300A (en) 1999-05-05 2000-05-05 Synthesizing combinatorial libraries of materials

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/305,830 1999-05-05
US09/305,830 US6507945B1 (en) 1999-05-05 1999-05-05 Synthesizing combinatorial libraries of materials

Publications (1)

Publication Number Publication Date
WO2000067086A1 true WO2000067086A1 (en) 2000-11-09

Family

ID=23182549

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/012228 WO2000067086A1 (en) 1999-05-05 2000-05-05 Synthesizing combinatorial libraries of materials

Country Status (9)

Country Link
US (1) US6507945B1 (en)
EP (1) EP1175645B1 (en)
JP (1) JP2002542943A (en)
AT (1) ATE245286T1 (en)
AU (1) AU4701300A (en)
CA (1) CA2370573A1 (en)
DE (1) DE60003913T2 (en)
DK (1) DK1175645T3 (en)
WO (1) WO2000067086A1 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6507945B1 (en) 1999-05-05 2003-01-14 Symyx Technologies, Inc. Synthesizing combinatorial libraries of materials
EP1394697A2 (en) * 2002-08-30 2004-03-03 Siemens Aktiengesellschaft Method for processing data
US6758951B2 (en) 2001-10-11 2004-07-06 Symyx Technologies, Inc. Synthesis and characterization of materials for electrochemical cells
US6928877B2 (en) 2002-05-24 2005-08-16 Symyx Technologies, Inc. High throughput microbalance and methods of using same
US6939515B2 (en) 2001-08-10 2005-09-06 Symyx Technologies, Inc. Apparatuses and methods for creating and testing pre-formulations and systems for same
WO2011100737A3 (en) * 2010-02-15 2012-01-05 Siemens Medical Solutions Usa, Inc. Intuitive graphical user interface for carrying out chemical reactions
GB2495878A (en) * 2007-12-21 2013-04-24 Fisher Rosemount Systems Inc Presenting process recipe phase information
EP2801876A1 (en) * 2013-05-09 2014-11-12 Mitutoyo Corporation System, GUI and method for editing step and repeat operation instructions in a machine vision inspection system

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7199809B1 (en) 1998-10-19 2007-04-03 Symyx Technologies, Inc. Graphic design of combinatorial material libraries
US7216113B1 (en) * 2000-03-24 2007-05-08 Symyx Technologies, Inc. Remote Execution of Materials Library Designs
US6983233B1 (en) 2000-04-19 2006-01-03 Symyx Technologies, Inc. Combinatorial parameter space experiment design
US6664067B1 (en) 2000-05-26 2003-12-16 Symyx Technologies, Inc. Instrument for high throughput measurement of material physical properties and method of using same
US20020052882A1 (en) * 2000-07-07 2002-05-02 Seth Taylor Method and apparatus for visualizing complex data sets
US6678619B2 (en) * 2000-09-20 2004-01-13 Victor S. Lobanov Method, system, and computer program product for encoding and building products of a virtual combinatorial library
WO2002048841A2 (en) * 2000-12-15 2002-06-20 Symyx Technologies, Inc. Methods and apparatus for designing high-dimensional combinatorial experiments
US6658429B2 (en) * 2001-01-05 2003-12-02 Symyx Technologies, Inc. Laboratory database system and methods for combinatorial materials research
US7085773B2 (en) * 2001-01-05 2006-08-01 Symyx Technologies, Inc. Laboratory database system and methods for combinatorial materials research
US20020143725A1 (en) * 2001-01-29 2002-10-03 Smith Robin Young Systems, methods and computer program products for determining parameters for chemical synthesis and for supplying the reagents, equipment and/or chemicals synthesized thereby
US7250950B2 (en) * 2001-01-29 2007-07-31 Symyx Technologies, Inc. Systems, methods and computer program products for determining parameters for chemical synthesis
US6881363B2 (en) * 2001-12-07 2005-04-19 Symyx Technologies, Inc. High throughput preparation and analysis of materials
US20030149933A1 (en) * 2002-02-01 2003-08-07 Marco Falcioni Graphical design of chemical discovery processes
AU2003220147A1 (en) 2002-03-11 2003-09-29 Meso Scale Technologies, Llc. System and method for flexibly representing and processing assay plates
US7491367B2 (en) * 2002-06-04 2009-02-17 Applera Corporation System and method for providing a standardized state interface for instrumentation
JP2005534927A (en) * 2002-08-06 2005-11-17 エスエスシーアイ,インコーポレイティド Comparison method of X-ray diffraction patterns using fundamental parameter method
US20040123650A1 (en) * 2002-09-17 2004-07-01 Symyx Technologies, Inc. High throughput rheological testing of materials
US7213034B2 (en) * 2003-01-24 2007-05-01 Symyx Technologies, Inc. User-configurable generic experiment class for combinatorial materials research
WO2004078131A2 (en) * 2003-03-01 2004-09-16 Symyx Technologies, Inc. Evaluating effects of exposure conditions on drug samples over time
US7210332B2 (en) * 2003-03-21 2007-05-01 Symyx Technologies, Inc. Mechanical resonator
US7721590B2 (en) 2003-03-21 2010-05-25 MEAS France Resonator sensor assembly
US7840943B2 (en) * 2003-08-08 2010-11-23 Oracle America, Inc. Method and apparatus for transferring data in a distributed testing system
US20050187918A1 (en) * 2003-12-01 2005-08-25 George Fitzgerald Method of storing fast throughput experimentation information in a database
US20050273272A1 (en) * 2004-04-22 2005-12-08 Applera Corporation, A Delaware Corporation System and method for laboratory-wide information management
US20060048128A1 (en) * 2004-09-01 2006-03-02 Roth Steven T Module preparation scripts
US7705157B2 (en) * 2004-12-16 2010-04-27 Symyx Solutions, Inc. Phenol-heterocyclic ligands, metal complexes, and their uses as catalysts
US20070050092A1 (en) * 2005-08-12 2007-03-01 Symyx Technologies, Inc. Event-based library process design
CN101356006B (en) 2005-12-16 2013-01-30 陶氏环球技术有限责任公司 Polydentate heteroatom ligand containing metal complexes, catalysts and methods of making and using the same
US7631286B2 (en) 2005-12-30 2009-12-08 Wafertech Llc Automated metrology recipe generation
US20090286944A1 (en) * 2008-05-15 2009-11-19 Symyx Technologies, Inc. Select phenol-heterocycle ligands, metal complexes formed therefrom, and their uses as catalysts
US10699223B1 (en) * 2009-08-13 2020-06-30 Amazon Technologies, Inc. System and method for resource allocation across distinct processes of a materials handling facility
US10013252B2 (en) * 2010-04-16 2018-07-03 Oracle International Corporation Software development compliance system

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614608A (en) * 1995-01-20 1997-03-25 Selectide Corporation Apparatus and method for multiple synthesis of organic compounds on polymer support
WO1998012559A1 (en) * 1996-09-20 1998-03-26 Demers James P Spatially addressable combinatorial chemical arrays in cd-rom format
WO1998014641A1 (en) * 1996-10-02 1998-04-09 Symyx Technologies Potential masking systems and methods for combinatorial library synthesis
US5776359A (en) * 1994-10-18 1998-07-07 Symyx Technologies Giant magnetoresistive cobalt oxide compounds
WO1998047613A1 (en) * 1997-04-22 1998-10-29 Symyx Technologies Systems and methods for the combinatorial synthesis of novel materials

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4901221A (en) 1986-04-14 1990-02-13 National Instruments, Inc. Graphical system for modelling a process and associated method
US5291587A (en) 1986-04-14 1994-03-01 National Instruments, Inc. Graphical system for executing a process and for programming a computer to execute a process, including graphical variable inputs and variable outputs
US4914568A (en) 1986-10-24 1990-04-03 National Instruments, Inc. Graphical system for modelling a process and associated method
US4829445A (en) * 1987-03-11 1989-05-09 National Semiconductor Corporation Distributed routing unit for fully-automated flexible manufacturing system
EP0496785B1 (en) 1989-10-17 1997-03-26 The Perkin-Elmer Corporation Robotic interface
US5355304A (en) 1990-01-30 1994-10-11 Demoranville Victoria E Clinical laboratory work-flow system which semi-automates validated immunoassay and electrophoresis protocols
WO1991016675A1 (en) 1990-04-06 1991-10-31 Applied Biosystems, Inc. Automated molecular biology laboratory
US5499193A (en) 1991-04-17 1996-03-12 Takeda Chemical Industries, Ltd. Automated synthesis apparatus and method of controlling the apparatus
US5506787A (en) 1992-08-31 1996-04-09 Siemens Aktiengesellschaft Configurable man-machine communication structure for machine tool or robot control systems
US5576946A (en) 1993-09-30 1996-11-19 Fluid Air, Inc. Icon based process design and control system
US6034681A (en) * 1993-12-17 2000-03-07 International Business Machines Corp. Dynamic data link interface in a graphic user interface
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US5694546A (en) * 1994-05-31 1997-12-02 Reisman; Richard R. System for automatic unattended electronic information transport between a server and a client by a vendor provided transport software with a manifest list
US5546301A (en) 1994-07-19 1996-08-13 Honeywell Inc. Advanced equipment control system
US5611059A (en) 1994-09-02 1997-03-11 Square D Company Prelinked parameter configuration, automatic graphical linking, and distributed database configuration for devices within an automated monitoring/control system
US5623592A (en) 1994-10-18 1997-04-22 Molecular Dynamics Method and apparatus for constructing an iconic sequence to operate external devices
US6030917A (en) * 1996-07-23 2000-02-29 Symyx Technologies, Inc. Combinatorial synthesis and analysis of organometallic compounds and catalysts
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
GB9501378D0 (en) * 1995-01-24 1995-03-15 Ibm A system and method for establishing a communication channel over a heterogeneous network between a source node and a destination node
US5737498A (en) 1995-07-11 1998-04-07 Beckman Instruments, Inc. Process automation method and apparatus
US5812394A (en) 1995-07-21 1998-09-22 Control Systems International Object-oriented computer program, system, and method for developing control schemes for facilities
US5880972A (en) 1996-02-26 1999-03-09 Pharmacopeia, Inc. Method and apparatus for generating and representing combinatorial chemistry libraries
CA2198433A1 (en) 1996-03-22 1997-09-23 Sheila Helen Hobbs Dewitt Information management system for automated multiple simultaneous synthesis
US5801942A (en) 1996-04-12 1998-09-01 Fisher-Rosemount Systems, Inc. Process control system user interface including selection of multiple control languages
US5862052A (en) 1996-04-12 1999-01-19 Fisher-Rosemount Systems, Inc. Process control system using a control strategy implemented in a layered hierarchy of control modules
US5960200A (en) * 1996-05-03 1999-09-28 I-Cube System to transition an enterprise to a distributed infrastructure
US6175816B1 (en) 1997-05-23 2001-01-16 Advanced Life Sciences, Inc. Use of automated technology in chemical process research and development
US6044212A (en) 1996-05-24 2000-03-28 Advanced Life Sciences, Inc. Use of automated technology in chemical process research and development
US5781190A (en) * 1996-06-05 1998-07-14 Microsoft Corporation Method and system for transferring a slide presentation between computers
ATE347101T1 (en) 1996-10-07 2006-12-15 Discovery Partners Internation SYSTEM FOR SYNTHESIS OF A COMPOUND LIBRARY AND USE THEREOF
US6088693A (en) * 1996-12-06 2000-07-11 International Business Machines Corporation Data management system for file and database management
US6014670A (en) * 1997-11-07 2000-01-11 Informatica Corporation Apparatus and method for performing data transformations in data warehousing
US6339775B1 (en) * 1997-11-07 2002-01-15 Informatica Corporation Apparatus and method for performing data transformations in data warehousing
US6003040A (en) * 1998-01-23 1999-12-14 Mital; Vijay Apparatus and method for storing, navigating among and adding links between data items in computer databases
US6339795B1 (en) * 1998-09-24 2002-01-15 Egrabber, Inc. Automatic transfer of address/schedule/program data between disparate data hosts
US6240335B1 (en) * 1998-12-14 2001-05-29 Palo Alto Technologies, Inc. Distributed control system architecture and method for a material transport system
US6507945B1 (en) 1999-05-05 2003-01-14 Symyx Technologies, Inc. Synthesizing combinatorial libraries of materials

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776359A (en) * 1994-10-18 1998-07-07 Symyx Technologies Giant magnetoresistive cobalt oxide compounds
US5614608A (en) * 1995-01-20 1997-03-25 Selectide Corporation Apparatus and method for multiple synthesis of organic compounds on polymer support
WO1998012559A1 (en) * 1996-09-20 1998-03-26 Demers James P Spatially addressable combinatorial chemical arrays in cd-rom format
WO1998014641A1 (en) * 1996-10-02 1998-04-09 Symyx Technologies Potential masking systems and methods for combinatorial library synthesis
WO1998047613A1 (en) * 1997-04-22 1998-10-29 Symyx Technologies Systems and methods for the combinatorial synthesis of novel materials

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6507945B1 (en) 1999-05-05 2003-01-14 Symyx Technologies, Inc. Synthesizing combinatorial libraries of materials
US6939515B2 (en) 2001-08-10 2005-09-06 Symyx Technologies, Inc. Apparatuses and methods for creating and testing pre-formulations and systems for same
US7549978B2 (en) 2001-08-10 2009-06-23 Symyx Technologies, Inc. Needle assembly
US6758951B2 (en) 2001-10-11 2004-07-06 Symyx Technologies, Inc. Synthesis and characterization of materials for electrochemical cells
US6928877B2 (en) 2002-05-24 2005-08-16 Symyx Technologies, Inc. High throughput microbalance and methods of using same
US7207211B2 (en) 2002-05-24 2007-04-24 Symyx Technologies, Inc. High throughput microbalance and methods of using same
EP1394697A2 (en) * 2002-08-30 2004-03-03 Siemens Aktiengesellschaft Method for processing data
EP1394697A3 (en) * 2002-08-30 2009-12-09 Siemens Aktiengesellschaft Method for processing data
GB2495878A (en) * 2007-12-21 2013-04-24 Fisher Rosemount Systems Inc Presenting process recipe phase information
GB2495878B (en) * 2007-12-21 2013-07-10 Fisher Rosemount Systems Inc Methods and apparatus to present recipe progress status information
WO2011100737A3 (en) * 2010-02-15 2012-01-05 Siemens Medical Solutions Usa, Inc. Intuitive graphical user interface for carrying out chemical reactions
EP2801876A1 (en) * 2013-05-09 2014-11-12 Mitutoyo Corporation System, GUI and method for editing step and repeat operation instructions in a machine vision inspection system
US9235337B2 (en) 2013-05-09 2016-01-12 Mitutoyo Corporation System, GUI and method for editing step and repeat operation instructions in a machine vision inspection system

Also Published As

Publication number Publication date
ATE245286T1 (en) 2003-08-15
AU4701300A (en) 2000-11-17
US6507945B1 (en) 2003-01-14
DE60003913T2 (en) 2004-02-05
DE60003913D1 (en) 2003-08-21
DK1175645T3 (en) 2003-11-10
EP1175645A1 (en) 2002-01-30
JP2002542943A (en) 2002-12-17
EP1175645B1 (en) 2003-07-16
CA2370573A1 (en) 2000-11-09

Similar Documents

Publication Publication Date Title
EP1175645B1 (en) Synthesizing combinatorial libraries of materials
US7937502B2 (en) Instrumentation control software
US7199809B1 (en) Graphic design of combinatorial material libraries
EP1188126B1 (en) Methods, apparatus, articles of manufacture, and user interfaces for performing automated biological assay preparation and macromolecule purification
US7024281B1 (en) Software for the controlled sampling of arrayed materials
CN104718456B (en) Laboratory machine and the method for automatically processing laboratory sample
US7908285B2 (en) User-configurable generic experiment class for combinatorial material research
CA2198433A1 (en) Information management system for automated multiple simultaneous synthesis
EP1080435B1 (en) Design of combinatorial material libraries
US6983233B1 (en) Combinatorial parameter space experiment design
EP1323008A2 (en) Automated process control and data management system and methods
WO2000052646A2 (en) A method and apparatus for automation of laboratory experimentation
EP1706800B1 (en) Method and apparatus for the development of a control process for an instrument
Kuo et al. A planning module for performing grid search, factorial design, and related combinatorial studies on an automated chemistry workstation
Du et al. An automated microscale chemistry workstation capable of parallel, adaptive experimentation
Beggs et al. The high throughput screening infrastructure: the right tools for the task
US20030149933A1 (en) Graphical design of chemical discovery processes
Cork et al. Further development of a versatile microscale automated workstation for parallel adaptive experimentation
AU2006203151B9 (en) Method and Apparatus for the Development of a Control Process for an Instrument
Hardin et al. Automating combinatorial chemistry: challenges and pitfalls
JP2001002595A (en) Automated chemical compound synthesizer
He et al. A user-friendly operating environment for an automatic life science laboratory reagent manipulating system
Seo et al. Kdas: general-purpose data acquisition system developed for KAIST-Tokamak
Hogan et al. A Solution for Discontinuous Sample Splitting and Dilution with the Biomek (r) 2000

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2370573

Country of ref document: CA

Ref country code: CA

Ref document number: 2370573

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2000928837

Country of ref document: EP

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 615862

Kind code of ref document: A

Format of ref document f/p: F

WWP Wipo information: published in national office

Ref document number: 2000928837

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 2000928837

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2000928837

Country of ref document: EP