WO2001001143A3 - Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker - Google Patents

Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker Download PDF

Info

Publication number
WO2001001143A3
WO2001001143A3 PCT/US2000/017422 US0017422W WO0101143A3 WO 2001001143 A3 WO2001001143 A3 WO 2001001143A3 US 0017422 W US0017422 W US 0017422W WO 0101143 A3 WO0101143 A3 WO 0101143A3
Authority
WO
WIPO (PCT)
Prior art keywords
attachment
biomolecules
reactive sites
solid supports
solid support
Prior art date
Application number
PCT/US2000/017422
Other languages
French (fr)
Other versions
WO2001001143A2 (en
Inventor
Travis Johnson
John Mcgowen
Allyson Beuhler
Charles Kimball Brush
Robert Emil Lajos
Original Assignee
Motorola Inc
Travis Johnson
John Mcgowen
Allyson Beuhler
Charles Kimball Brush
Robert Emil Lajos
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23351266&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2001001143(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Motorola Inc, Travis Johnson, John Mcgowen, Allyson Beuhler, Charles Kimball Brush, Robert Emil Lajos filed Critical Motorola Inc
Priority to EP00941693A priority Critical patent/EP1190254B1/en
Priority to JP2001507097A priority patent/JP2003524150A/en
Priority to DE60013826T priority patent/DE60013826D1/en
Priority to AT00941693T priority patent/ATE276516T1/en
Priority to CA002378072A priority patent/CA2378072A1/en
Priority to AU56362/00A priority patent/AU768326B2/en
Publication of WO2001001143A2 publication Critical patent/WO2001001143A2/en
Publication of WO2001001143A3 publication Critical patent/WO2001001143A3/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Abstract

The present invention provides solid supports (e.g., glass) and polymer hydrogels (particularly polymer hydrogel arrays present on a solid support) comprising one or more reactive sites for the attachment of biomolecules, as well as biomolecules comprising one or more reactive sites for attachment to solid supports and polymer hydrogels. The invention further provides novel compositions and methods for the preparation of biomolecules, solid supports and polymer hydrogels comprising reactive sites. The invention also provides for preparation of crosslinked solid supports, polymer hydrogels, and hydrogel arrays, wherein one or more biomolecules is attached by means of the reactive sites in a photocycloaddition reaction. Advantageously, according to the invention, crosslinking of the hydrogel and attachment of biomolecules can be done in a single step.
PCT/US2000/017422 1999-06-25 2000-06-23 Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker WO2001001143A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP00941693A EP1190254B1 (en) 1999-06-25 2000-06-23 Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker
JP2001507097A JP2003524150A (en) 1999-06-25 2000-06-23 Methods and compositions for binding biomolecules to solid supports, hydrogels, and hydrogel arrays
DE60013826T DE60013826D1 (en) 1999-06-25 2000-06-23 FASTENING A BIOMOLECULE TO A POLYMERIC SOLID PHASE BY CYCLOADDITION REACTIONS FROM A SPACER
AT00941693T ATE276516T1 (en) 1999-06-25 2000-06-23 ATTACHMENT OF A BIOMOLECULE TO A POLYMERIC SOLID PHASE THROUGH CYCLOADDITION REACTIONS OF A SPACER
CA002378072A CA2378072A1 (en) 1999-06-25 2000-06-23 Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
AU56362/00A AU768326B2 (en) 1999-06-25 2000-06-23 Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/344,620 1999-06-25
US09/344,620 US6372813B1 (en) 1999-06-25 1999-06-25 Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays

Publications (2)

Publication Number Publication Date
WO2001001143A2 WO2001001143A2 (en) 2001-01-04
WO2001001143A3 true WO2001001143A3 (en) 2001-03-08

Family

ID=23351266

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/017422 WO2001001143A2 (en) 1999-06-25 2000-06-23 Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker

Country Status (8)

Country Link
US (2) US6372813B1 (en)
EP (1) EP1190254B1 (en)
JP (1) JP2003524150A (en)
AT (1) ATE276516T1 (en)
AU (1) AU768326B2 (en)
CA (1) CA2378072A1 (en)
DE (1) DE60013826D1 (en)
WO (1) WO2001001143A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100876256B1 (en) * 2001-03-30 2008-12-26 유씨비 소시에떼아노님 Substrate, its manufacturing method and uses

Families Citing this family (236)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7527928B2 (en) * 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US7432048B2 (en) * 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US7638464B2 (en) 1999-04-26 2009-12-29 Biocept, Inc. Three dimensional format biochips
US6921638B2 (en) 1999-06-25 2005-07-26 Amersham Biosciences Ab Hydrogel-based microarray signal amplification methods and devices therefor
US6372813B1 (en) * 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
US6664061B2 (en) * 1999-06-25 2003-12-16 Amersham Biosciences Ab Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
US6589778B1 (en) * 1999-12-15 2003-07-08 Amersham Biosciences Ab Method and apparatus for performing biological reactions on a substrate surface
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) * 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
CA2399733C (en) * 2000-02-07 2011-09-20 Illumina, Inc. Nucleic acid detection methods using universal priming
US6693033B2 (en) 2000-02-10 2004-02-17 Motorola, Inc. Method of removing an amorphous oxide from a monocrystalline surface
DE10006590B4 (en) * 2000-02-11 2007-10-18 Qiagen North American Holdings, Inc. Use of functionalized membranes or matrices for the purification of nucleic acids and corresponding methods
WO2002003437A1 (en) * 2000-06-30 2002-01-10 Motorola, Inc., A Corporation Of The State Of Delaware Hybrid semiconductor structure and device
US6555946B1 (en) 2000-07-24 2003-04-29 Motorola, Inc. Acoustic wave device and process for forming the same
AU2001279244A1 (en) * 2000-08-09 2002-02-18 Motorola, Inc. The use and evaluation of a (2+2) photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
US6638838B1 (en) 2000-10-02 2003-10-28 Motorola, Inc. Semiconductor structure including a partially annealed layer and method of forming the same
KR20020089315A (en) * 2000-10-26 2002-11-29 바이오셉트 인코포레이티드 Three dimensional format biochips
DE60033665T2 (en) * 2000-10-31 2007-10-31 Micronas Gmbh Patterned polymer surfaces suitable for bioconjugations and methods for their preparation
US6673646B2 (en) 2001-02-28 2004-01-06 Motorola, Inc. Growth of compound semiconductor structures on patterned oxide films and process for fabricating same
US20020142304A1 (en) * 2001-03-09 2002-10-03 Anderson Daniel G. Uses and methods of making microarrays of polymeric biomaterials
DE50113981D1 (en) * 2001-05-09 2008-06-26 Andreas Hofmann OBJECT WITH AN UNLOCKED, FUNCTIONALIZED HYDROGEL SURFACE
US6709989B2 (en) 2001-06-21 2004-03-23 Motorola, Inc. Method for fabricating a semiconductor structure including a metal oxide interface with silicon
AU2002365110A1 (en) * 2001-07-10 2003-07-15 Massachusetts Institute Of Technology Small molecule microarrays
US6646293B2 (en) 2001-07-18 2003-11-11 Motorola, Inc. Structure for fabricating high electron mobility transistors utilizing the formation of complaint substrates
US6693298B2 (en) 2001-07-20 2004-02-17 Motorola, Inc. Structure and method for fabricating epitaxial semiconductor on insulator (SOI) structures and devices utilizing the formation of a compliant substrate for materials used to form same
US6667196B2 (en) 2001-07-25 2003-12-23 Motorola, Inc. Method for real-time monitoring and controlling perovskite oxide film growth and semiconductor structure formed using the method
US6639249B2 (en) 2001-08-06 2003-10-28 Motorola, Inc. Structure and method for fabrication for a solid-state lighting device
US6589856B2 (en) 2001-08-06 2003-07-08 Motorola, Inc. Method and apparatus for controlling anti-phase domains in semiconductor structures and devices
US6656725B2 (en) * 2001-08-15 2003-12-02 The University Of Chicago Method of fabrication of microarray of gel-immobilized compounds on a chip by copolymerization
US6673667B2 (en) 2001-08-15 2004-01-06 Motorola, Inc. Method for manufacturing a substantially integral monolithic apparatus including a plurality of semiconductor materials
CA2457142A1 (en) 2001-08-17 2003-02-27 University Of Pittsburgh Administration of estradiol metabolites for the treatment or prevention of obesity, metabolic syndrome, diabetes, and vascular and renal disorders
US6943267B1 (en) 2001-08-24 2005-09-13 Utah State University Thiophosphonate inhibitors of phosphatase enzymes and metallophosphatases
US20080279936A1 (en) * 2001-10-10 2008-11-13 The University Of Pittsburgh Estradiol metabolites for reduction of endothelin production
WO2003034026A2 (en) * 2001-10-15 2003-04-24 Biocept, Inc. Microwell biochip
EP2287330A1 (en) * 2001-11-01 2011-02-23 Rensselaer Polytechnic Institute Biocatalytic Solgel Microarrays
AU2002351164A1 (en) * 2001-11-26 2003-06-10 Molecular Reflections, Inc. Microscale immobilization of molecules using a hydrogel and methods of use thereof
KR100700462B1 (en) * 2001-12-26 2007-03-29 캐논 가부시끼가이샤 Probe Medium
US20050239155A1 (en) * 2002-01-04 2005-10-27 Javier Alarcon Entrapped binding protein as biosensors
HU226993B1 (en) * 2002-03-29 2010-04-28 Mta Szegedi Biolog Koezpont Novel active carrier and process for immobilization of combinatorial compounds and compound libraries
US6916717B2 (en) * 2002-05-03 2005-07-12 Motorola, Inc. Method for growing a monocrystalline oxide layer and for fabricating a semiconductor device on a monocrystalline substrate
US7445894B2 (en) * 2002-05-03 2008-11-04 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
JP2005539243A (en) * 2002-05-03 2005-12-22 モレキュラー プローブス, インコーポレイテッド Compositions and methods for detection and separation of phosphorylated molecules
US20040171034A1 (en) * 2002-05-03 2004-09-02 Brian Agnew Compositions and methods for detection and isolation of phosphorylated molecules
US6706408B2 (en) * 2002-05-16 2004-03-16 Surmodics, Inc. Silane coating composition
US8785179B2 (en) * 2002-05-22 2014-07-22 Texas Instruments Incorporated Biosensor and method
US20030219753A1 (en) * 2002-05-22 2003-11-27 Quinn John G. Biosensor and method
US20050019747A1 (en) * 2002-08-07 2005-01-27 Anderson Daniel G. Nanoliter-scale synthesis of arrayed biomaterials and screening thereof
US20040028804A1 (en) * 2002-08-07 2004-02-12 Anderson Daniel G. Production of polymeric microarrays
EP1527202A1 (en) * 2002-08-08 2005-05-04 Siemens Aktiengesellschaft Recognition layers made of hydrogel based on polyacrylamide for use in biosensor technology
US7045366B2 (en) * 2003-09-12 2006-05-16 Ciphergen Biosystems, Inc. Photocrosslinked hydrogel blend surface coatings
US8389700B2 (en) * 2002-09-23 2013-03-05 Alcatel Lucent Agent delivery system capable of selectively releasing an agent
US7919600B2 (en) * 2002-09-23 2011-04-05 Alcatel-Lucent Usa Inc. Compositions that reversibly gel and de-gel
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US20040152211A1 (en) * 2002-11-15 2004-08-05 The Regents Of The University Of California System and method for multiplexed biomolecular analysis
JP2006507122A (en) * 2002-11-22 2006-03-02 カーネギー−メロン ユニバーシティ Compositions and methods for reversible capture of biomolecules
DE10259821B4 (en) * 2002-12-19 2006-03-09 Siemens Ag Biochip
BRPI0407667A (en) * 2003-02-20 2006-03-01 Univ Pittsburgh estradiol metabolites for the treatment of pulmonary hypertension
US20060194775A1 (en) * 2003-02-20 2006-08-31 University Of Pittsburgh Estradiol metabolites for the treatment of pulmonary hypertension
EP1615034A4 (en) * 2003-03-31 2010-11-24 Rikagaku Kenkyusho Fixing agents, method of fixing substance with the same, and substrate having substance fixed thereto with the same
DE10323685A1 (en) * 2003-05-22 2004-12-09 Rühe, Jürgen, Prof. Dr. Process for the covalent immobilization of probe biomolecules on organic surfaces
US20050136536A1 (en) * 2003-09-15 2005-06-23 Anderson Daniel G. Embryonic epithelial cells
CA2543091C (en) 2003-10-24 2013-09-03 John Austin Apparatus and method for dispensing fluid, semi-solid and solid samples
DE102004062281A1 (en) * 2003-12-29 2005-07-28 Siemens Ag Producing a microarray of spots e.g. for use on a biochip, comprises applying a solution of a polymer and special molecules and then solidifying the polymer, especially by a non-radical method
EP3673986A1 (en) 2004-01-07 2020-07-01 Illumina Cambridge Limited Improvements in or relating to molecular arrays
US7294671B2 (en) * 2004-02-06 2007-11-13 Invista North America S.A.R.L. Reactive carriers for polymer melt injection
DE102004010430A1 (en) * 2004-03-01 2005-09-22 Rühe, Jürgen, Prof. Dr. Process for the covalent immobilization of biomolecules on organic surfaces
US20090042741A1 (en) * 2004-05-06 2009-02-12 Arizona Board Of Regents, Acting For And On Behalf Of Arizona State University Microarray of three-dimensional heteropolymer microstructures and method therefor
US7968085B2 (en) 2004-07-05 2011-06-28 Ascendis Pharma A/S Hydrogel formulations
US7935479B2 (en) * 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
US7846676B2 (en) 2004-07-19 2010-12-07 Cell Biosciences, Inc. Methods and devices for analyte detection
US7595157B2 (en) * 2004-08-19 2009-09-29 Biocept, Inc. Microarrays utilizing hydrogels
KR100695134B1 (en) * 2004-11-25 2007-03-14 삼성전자주식회사 Microarray using laminar flow and preparation method thereof
US20070287828A1 (en) * 2005-02-18 2007-12-13 Minden Jonathan S Compositions and Methods for the Reversible Capture of Biomolecules
WO2006127865A2 (en) * 2005-05-24 2006-11-30 Georgia Tech Research Corporation Bioresponsive hydrogels
WO2006138479A2 (en) * 2005-06-15 2006-12-28 Arizona Board Of Regents For And On Behalf Of Arizona State University Microstructure and microdomain microarrays, methods of making and using thereof
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US20070054127A1 (en) * 2005-08-26 2007-03-08 Hergenrother Robert W Silane coating compositions, coating systems, and methods
US20070141365A1 (en) * 2005-08-26 2007-06-21 Jelle Bruce M Silane Coating Compositions, Coating Systems, and Methods
CA2622955A1 (en) * 2005-09-15 2007-03-29 University Of Utah Research Foundation Polymeric compositions and methods of making and using thereof
WO2007035864A2 (en) * 2005-09-20 2007-03-29 Cell Biosciences, Inc. Electrophoresis standards, methods and kits
KR101375086B1 (en) * 2005-11-08 2014-03-17 한국과학기술원 Hyperbranched polymer for micro devices
US8158832B2 (en) 2005-11-09 2012-04-17 The Trustees Of Columbia University In The City Of New York Photochemical methods and photoactive compounds for modifying surfaces
DE102005059535B4 (en) * 2005-12-13 2010-01-14 Siemens Ag Device and method for carrying out a nucleic acid test, and method for producing such a device
EP1864777B1 (en) * 2006-06-08 2009-09-23 DWI an der RWTH Aachen e.V. Structuring hydrogels
HUP0600668A2 (en) 2006-08-22 2008-02-28 Avicor Kft Active carrier, process for producing thereof and the use of thereof
US8658573B2 (en) * 2006-09-11 2014-02-25 The Trustees Of Columbia University In The City Of New York Photo-generated carbohydrate arrays and the rapid identification of pathogen-specific antigens and antibodies
US10107782B2 (en) 2008-01-25 2018-10-23 ProteinSimple Method to perform limited two dimensional separation of proteins and other biologicals
JP2011523452A (en) * 2008-04-16 2011-08-11 スマート ホログラムズ リミテッド Photopolymerizable composition
US8993068B2 (en) 2008-11-04 2015-03-31 The Trustees Of Columbia University In The City Of New York Heterobifunctional polymers and methods for layer-by-layer construction of multilayer films
EP2435461B1 (en) 2009-05-29 2017-08-09 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
NZ597964A (en) 2009-07-31 2014-04-30 Sanofi Aventis Deutschland Long acting insulin composition
JP5738291B2 (en) 2009-07-31 2015-06-24 サノフィ−アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Prodrugs containing insulin linker conjugates
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2438930A1 (en) 2010-09-17 2012-04-11 Sanofi-Aventis Deutschland GmbH Prodrugs comprising an exendin linker conjugate
AU2011312218B2 (en) 2010-10-04 2015-11-05 Sequencing Health, Inc. Systems and methods for automated reusable parallel biological reactions
US9006345B2 (en) 2011-03-25 2015-04-14 The Trustees Of Columbia University In The City Of New York Heterotrifunctional molecules and methods for the synthesis of dendrimeric materials
JP2014512826A (en) 2011-04-25 2014-05-29 バイオ−ラド ラボラトリーズ インコーポレイテッド Methods and compositions for nucleic acid analysis
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2718465B1 (en) 2011-06-09 2022-04-13 Illumina, Inc. Method of making an analyte array
WO2013006824A2 (en) 2011-07-07 2013-01-10 Life Technologies Corporation Polymer particles, nucleic acid polymer particles and methods of making and using the same
EP2756096B1 (en) 2011-09-12 2017-06-28 Ecole Polytechnique Fédérale de Lausanne (EPFL) Method for preparing topographically structured microarrays
EP2771103B1 (en) 2011-10-28 2017-08-16 Illumina, Inc. Microarray fabrication system and method
WO2013067203A1 (en) 2011-11-02 2013-05-10 The Regents Of The University Of California Reprogramming of cellular adhesion
WO2013070627A2 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
CN106591103B (en) 2011-12-01 2021-06-04 吉纳普赛斯股份有限公司 System and method for efficient electronic sequencing and detection
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
WO2013155634A1 (en) 2012-04-19 2013-10-24 Jiaqi Wu Dual wavelength isoelectric focusing for determining drug load in antibody drug conjugates
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
MX364957B (en) 2012-08-14 2019-05-15 10X Genomics Inc Microcapsule compositions and methods.
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
DK2964612T3 (en) 2013-03-08 2017-04-03 Illumina Cambridge Ltd POLYMETHIN COMPOUNDS AND USE THEREOF AS FLUORESCING LABELS
ES2620427T3 (en) 2013-03-08 2017-06-28 Illumina Cambridge Limited Rhodamine compounds and their use as fluorescent markers
CN105051214B (en) 2013-03-15 2018-12-28 吉纳普赛斯股份有限公司 System and method for bioanalysis
KR102266002B1 (en) 2013-07-01 2021-06-16 일루미나, 인코포레이티드 Catalyst-free surface functionalization and polymer grafting
WO2015017694A1 (en) * 2013-07-31 2015-02-05 Regents Of The University Of California Dna double-write/double binding identity
SG10201806890VA (en) 2013-08-28 2018-09-27 Cellular Res Inc Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CN105658817A (en) 2013-09-13 2016-06-08 生命技术公司 Device preparation using condensed nucleic acid particles
US9766206B2 (en) 2013-09-27 2017-09-19 ProteinSimple Apparatus, systems, and methods for capillary electrophoresis
EP3080300B1 (en) 2013-12-11 2020-09-02 Genapsys Inc. Systems and methods for biological analysis and computation
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CN105874385B (en) 2013-12-19 2021-08-20 Illumina公司 Substrate comprising a nanopatterned surface and method for the production thereof
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
EP3129143B1 (en) 2014-04-10 2022-11-23 10X Genomics, Inc. Method for partitioning microcapsules
EP3556864B1 (en) 2014-04-18 2020-12-09 Genapsys, Inc. Methods and systems for nucleic acid amplification
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
CN106795553B (en) 2014-06-26 2021-06-04 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
CN110211637B (en) 2014-06-26 2023-10-27 10X基因组学有限公司 Method and system for assembling nucleic acid sequences
EP3212807B1 (en) 2014-10-29 2020-09-02 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US11754563B2 (en) * 2014-11-20 2023-09-12 Global Life Sciences Solutions Operations UK Ltd Porous membranes with a polymer grafting, methods and uses thereof
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
AU2016218254B2 (en) 2015-02-09 2019-12-19 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same
US10854315B2 (en) 2015-02-09 2020-12-01 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
EP3835431B1 (en) 2015-03-30 2022-11-02 Becton, Dickinson and Company Methods for combinatorial barcoding
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
ES2945607T3 (en) 2015-07-17 2023-07-04 Illumina Inc Polymer sheets for sequencing applications
KR102395450B1 (en) 2015-09-11 2022-05-09 셀룰러 리서치, 인크. Methods and Compositions for Normalizing Nucleic Acid Libraries
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
US20200292944A1 (en) 2015-11-18 2020-09-17 Massachusetts Institute Of Technology Method of making a patterned hydrogel and kit to make it
EP3384048B1 (en) 2015-12-04 2021-03-24 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
US11081208B2 (en) 2016-02-11 2021-08-03 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3458913B1 (en) 2016-05-18 2020-12-23 Illumina, Inc. Self assembled patterning using patterned hydrophobic surfaces
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
AU2017331459B2 (en) 2016-09-26 2023-04-13 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
ES2937782T3 (en) 2016-10-03 2023-03-31 Illumina Inc Fluorescent detection of amines and hydrazines and methods of testing the same
MX2019006705A (en) 2016-12-22 2019-11-08 Illumina Inc Arrays including a resin film and a patterned polymer layer.
EP3559262A4 (en) 2016-12-22 2020-07-22 Illumina, Inc. Arrays with quality control tracers
WO2018118932A1 (en) 2016-12-22 2018-06-28 Illumina, Inc. Imprinting apparatus
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018119057A2 (en) 2016-12-22 2018-06-28 Illumina, Inc. Array including sequencing primer and non-sequencing entity
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3559982A4 (en) 2016-12-22 2020-07-01 Illumina, Inc. Flow cell package and method for making the same
CN109476674B (en) 2016-12-22 2021-12-10 伊鲁米纳剑桥有限公司 Coumarin compound and application thereof as fluorescent marker
KR20190104041A (en) 2016-12-29 2019-09-05 아도르 디아그노스틱스 에스.알.엘. Electrophoresis chip for electrophoretic applications
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. Systems and methods for analyzing datasets
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
KR20220124280A (en) 2017-06-05 2022-09-13 백톤 디킨슨 앤드 컴퍼니 Sample indexing for single cells
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
SG11202002516WA (en) 2017-09-21 2020-04-29 Genapsys Inc Systems and methods for nucleic acid sequencing
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
ES2945191T3 (en) 2018-05-03 2023-06-29 Becton Dickinson Co High-throughput multi-omics sample analysis
JP2022511398A (en) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー Determining the 5'transcription sequence
KR102313444B1 (en) * 2018-10-15 2021-10-15 세종대학교산학협력단 Cellulose nanofiber comprising biocompatible inorganic material on the surface thereof and polymer composites having the same
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322A3 (en) 2019-01-23 2023-09-20 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
KR20210134210A (en) 2019-03-01 2021-11-09 일루미나 케임브리지 리미티드 Tertiary amine substituted coumarin compounds and their use as fluorescent labels
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
SG11202012555UA (en) 2019-03-01 2021-01-28 Illumina Cambridge Ltd Exocyclic amine-substituted coumarin compounds and their uses as fluorescent labels
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
KR20220107231A (en) 2019-11-27 2022-08-02 일루미나 케임브리지 리미티드 Dyes and compositions containing cyclooctatetraene
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
AU2021209907A1 (en) 2020-01-24 2022-07-07 Slingshot Biosciences, Inc. Compositions and methods for cell-like calibration particles
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
CN115867560A (en) 2020-06-22 2023-03-28 伊鲁米纳剑桥有限公司 Nucleosides and nucleotides having 3' acetal capping groups
US20220033900A1 (en) 2020-07-28 2022-02-03 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
WO2022096294A2 (en) 2020-11-03 2022-05-12 Droplet Genomics, Uab Integrated platform for selective microfluidic particle processing
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
EP4334327A1 (en) 2021-05-05 2024-03-13 Illumina Cambridge Limited Fluorescent dyes containing bis-boron fused heterocycles and uses in sequencing
CA3216735A1 (en) 2021-05-20 2022-11-24 Patrizia IAVICOLI Compositions and methods for sequencing by synthesis
WO2023288225A1 (en) * 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
CA3222829A1 (en) 2021-12-16 2023-06-22 Gabriele CANZI Methods for metal directed cleavage of surface-bound polynucleotides
CA3223274A1 (en) 2021-12-20 2023-06-29 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
CA3222842A1 (en) 2021-12-20 2023-06-29 Oliver MILLER Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
WO2023186815A1 (en) 2022-03-28 2023-10-05 Illumina Cambridge Limited Labeled avidin and methods for sequencing
AU2023246772A1 (en) 2022-03-29 2024-01-18 Illumina Inc. Chromenoquinoline dyes and uses in sequencing
AU2023246691A1 (en) 2022-03-30 2024-01-18 Illumina, Inc. Methods for chemical cleavage of surface-bound polynucleotides
WO2023192900A1 (en) 2022-03-31 2023-10-05 Illumina Singapore Pte. Ltd. Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis
WO2023186982A1 (en) 2022-03-31 2023-10-05 Illumina, Inc. Compositions and methods for improving sequencing signals
WO2023232829A1 (en) 2022-05-31 2023-12-07 Illumina, Inc Compositions and methods for nucleic acid sequencing
US20230416279A1 (en) 2022-06-28 2023-12-28 Illumina Cambridge Limited Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0386644A2 (en) * 1989-03-10 1990-09-12 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Reagents for the determination of drugs
DE3919915A1 (en) * 1989-06-19 1990-12-20 Boehringer Mannheim Gmbh AMINOALKYLMALEIMIDES AND DERIVED HAPTEN AND ANTIGEN DERIVATIVES AND CONJUGATES WITH PEPTIDES OR PROTEINS
US5582955A (en) * 1994-06-23 1996-12-10 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Chemical functionalization of surfaces

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826678A (en) * 1972-06-06 1974-07-30 Atomic Energy Commission Method for preparation of biocompatible and biofunctional materials and product thereof
US4315998A (en) * 1974-06-12 1982-02-16 Research Corporation Polymer-bound photosensitizing catalysts
GB8530715D0 (en) 1985-12-13 1986-01-22 Unilever Plc Microchemical testing
US5449602A (en) * 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
CA2028804C (en) * 1989-11-21 2000-06-20 Howard J. Buttery Biomosaic polymers and method for preparing same
US5410016A (en) * 1990-10-15 1995-04-25 Board Of Regents, The University Of Texas System Photopolymerizable biodegradable hydrogels as tissue contacting materials and controlled-release carriers
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
EP0585436B1 (en) * 1992-02-13 2000-05-03 SurModics, Inc. Immobilization of chemical species in crosslinked matrices
RU2041261C1 (en) 1993-08-11 1995-08-09 Институт молекулярной биологии им.В.А.Энгельгардта РАН Method for manufacturing of matrix for detecting of mismatches
US5736257A (en) 1995-04-25 1998-04-07 Us Navy Photoactivatable polymers for producing patterned biomolecular assemblies
US5990193A (en) * 1995-12-12 1999-11-23 University Of Pittsburgh Polymers for reversible photoinduced sol gel transitions
US5861247A (en) 1996-01-26 1999-01-19 University Of Chicago Rapid method to detect duplex formation in sequencing by hybridization methods
US5942555A (en) 1996-03-21 1999-08-24 Surmodics, Inc. Photoactivatable chain transfer agents and semi-telechelic photoactivatable polymers prepared therefrom
US5905024A (en) * 1996-12-17 1999-05-18 University Of Chicago Method for performing site-specific affinity fractionation for use in DNA sequencing
US5932711A (en) 1997-03-05 1999-08-03 Mosaic Technologies, Inc. Nucleic acid-containing polymerizable complex
US5858653A (en) 1997-09-30 1999-01-12 Surmodics, Inc. Reagent and method for attaching target molecules to a surface
US5962578A (en) 1997-11-19 1999-10-05 Amcol International Corporation Poly(dialkylaminoalkyl (meth)acrylamide)-based superabsorbent gels
US6391937B1 (en) 1998-11-25 2002-05-21 Motorola, Inc. Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers
US6174683B1 (en) * 1999-04-26 2001-01-16 Biocept, Inc. Method of making biochips and the biochips resulting therefrom
US6372813B1 (en) * 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
US6423818B1 (en) * 1999-07-30 2002-07-23 Takehisa Matsuda Coumarin endcapped absorbable polymers
US20020103348A1 (en) * 1999-12-27 2002-08-01 Fuji Photo Film Co., Ltd. DNA chip and its preparation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0386644A2 (en) * 1989-03-10 1990-09-12 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Reagents for the determination of drugs
DE3919915A1 (en) * 1989-06-19 1990-12-20 Boehringer Mannheim Gmbh AMINOALKYLMALEIMIDES AND DERIVED HAPTEN AND ANTIGEN DERIVATIVES AND CONJUGATES WITH PEPTIDES OR PROTEINS
US5582955A (en) * 1994-06-23 1996-12-10 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Chemical functionalization of surfaces

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOOKER-MILBURN K.I., COWELL J.K., JIMÉNEZ F.D., SHARPE A., WHITE A.J.: "Stereoselective Intermolecular [2+2] Photocycloaddition Reactions of Tetrahydrophthalic Anhydride and Derivatives with Alkenols and Alkynols", TETRAHEDRON, vol. 55, 1999, pages 5875 - 5887, XP002153920 *
WALKER M.A.: "The Mitsunobu Reaction: A novel Method for the Synthesis of Bifunctional Maleimide Linkers", TETRAHEDRON LETTERS, vol. 35, no. 5, 1994, pages 665 - 668, XP002153919 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100876256B1 (en) * 2001-03-30 2008-12-26 유씨비 소시에떼아노님 Substrate, its manufacturing method and uses

Also Published As

Publication number Publication date
WO2001001143A2 (en) 2001-01-04
AU5636200A (en) 2001-01-31
EP1190254A2 (en) 2002-03-27
AU768326B2 (en) 2003-12-11
JP2003524150A (en) 2003-08-12
US6372813B1 (en) 2002-04-16
ATE276516T1 (en) 2004-10-15
US6686161B2 (en) 2004-02-03
US20030078314A1 (en) 2003-04-24
CA2378072A1 (en) 2001-01-04
EP1190254B1 (en) 2004-09-15
DE60013826D1 (en) 2004-10-21

Similar Documents

Publication Publication Date Title
WO2001001143A3 (en) Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker
EP0940447A3 (en) Silicone hydrogel polymers
CA2266581A1 (en) Polymers containing polysaccharides such as alginates or modified alginates
AU3360197A (en) Hydrolysable hydrogels for controlled release
Zhang et al. Molecularly imprinted nanoreactors for regioselective huisgen 1, 3-dipolar cycloaddition reaction
AU2076401A (en) Characterization of hybridized polymer molecules based on monomer-interface interactions
WO2005112888A8 (en) Methods for making injectable polymer hydrogels
JPS61171712A (en) Graft copolymer consisting of crosslinked polymer and polyoxyethylene, its production and its use
WO2001048039A3 (en) Process for the preparation of ethylene polymers
US20100280184A1 (en) Surface functionalisation of plastic optical fibre
WO2004030135A3 (en) Proton-conducting membrane and use thereof verwendung
AU7261098A (en) Process for making surface modified porous membrane with perfluorocarbon copolymer
WO2000018914A3 (en) Novel dkr polypeptides
AU5060900A (en) Chemically modified lipolytic enzyme
CA2087730A1 (en) Use of crosslinked crystals as a novel form of enzyme immobilization
AU2831900A (en) Stereocomplex hydrogels
AU6872100A (en) Process for producing porous crosslinked polymer
CA2358848A1 (en) Aggregates having attached polymer groups and polymer foams
CN112707977B (en) Method for amination of polystyrene-based resin, and method for immobilizing enzyme on aminated resin
EP1053258B8 (en) Device for producing polymer support materials in the form of porous polymer beads
AU2003220516A1 (en) Polymer grafted support polymers
AU7280300A (en) Porous organic polymer shaped bodies
EP1806367A3 (en) Polymers containing polysaccharides such as alginates or modified alginates
WO2005056636A3 (en) Method of preparing maleimide functionalized polymers
WO2002092201A3 (en) Method for reducing an adsorption tendency of molecules or biological cells on a material surface

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2000941693

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2378072

Country of ref document: CA

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 507097

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 56362/00

Country of ref document: AU

WWP Wipo information: published in national office

Ref document number: 2000941693

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 56362/00

Country of ref document: AU

WWG Wipo information: grant in national office

Ref document number: 2000941693

Country of ref document: EP

DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)