WO2001007484A3 - Protease conjugates having sterically protected clip sites - Google Patents

Protease conjugates having sterically protected clip sites Download PDF

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Publication number
WO2001007484A3
WO2001007484A3 PCT/US2000/018855 US0018855W WO0107484A3 WO 2001007484 A3 WO2001007484 A3 WO 2001007484A3 US 0018855 W US0018855 W US 0018855W WO 0107484 A3 WO0107484 A3 WO 0107484A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
moiety
conjugates
protease conjugates
sterically protected
Prior art date
Application number
PCT/US2000/018855
Other languages
French (fr)
Other versions
WO2001007484A2 (en
Inventor
David John Weisgerber
Donn Nelton Rubingh
Paul Elliott Correa
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Priority to BR0012694-2A priority Critical patent/BR0012694A/en
Priority to AU59284/00A priority patent/AU5928400A/en
Priority to CA002379729A priority patent/CA2379729A1/en
Priority to JP2001512566A priority patent/JP2003516116A/en
Priority to MXPA02000842A priority patent/MXPA02000842A/en
Priority to KR1020027000932A priority patent/KR20020021395A/en
Priority to EP00945318A priority patent/EP1196548A2/en
Publication of WO2001007484A2 publication Critical patent/WO2001007484A2/en
Publication of WO2001007484A3 publication Critical patent/WO2001007484A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to a clip site protection position of the protease moiety, wherein the clip site protection positions are selected from 13, 14, 15, 16, 18, 19, 20, 21, 84, 85, 88, 158, 159, 160, 161, 162, 163, 164, 165, 170, 186, 191, 192, 193, 194, 196, 259, 260, 261, 262, and 274 corresponding to subtilisin BPN'. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.
PCT/US2000/018855 1999-07-22 2000-07-11 Protease conjugates having sterically protected clip sites WO2001007484A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
BR0012694-2A BR0012694A (en) 1999-07-22 2000-07-11 Protease conjugate, cleaning composition and personal treatment composition
AU59284/00A AU5928400A (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected clip sites
CA002379729A CA2379729A1 (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected clip sites
JP2001512566A JP2003516116A (en) 1999-07-22 2000-07-11 Protease conjugates with sterically protected clip sites
MXPA02000842A MXPA02000842A (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected clip sites.
KR1020027000932A KR20020021395A (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected clip sites
EP00945318A EP1196548A2 (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected clip sites

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14498199P 1999-07-22 1999-07-22
US60/144,981 1999-07-22

Publications (2)

Publication Number Publication Date
WO2001007484A2 WO2001007484A2 (en) 2001-02-01
WO2001007484A3 true WO2001007484A3 (en) 2001-06-07

Family

ID=22511058

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/018855 WO2001007484A2 (en) 1999-07-22 2000-07-11 Protease conjugates having sterically protected clip sites

Country Status (11)

Country Link
US (1) US6566115B1 (en)
EP (1) EP1196548A2 (en)
JP (1) JP2003516116A (en)
KR (1) KR20020021395A (en)
CN (1) CN1377406A (en)
AU (1) AU5928400A (en)
BR (1) BR0012694A (en)
CA (1) CA2379729A1 (en)
CZ (1) CZ2002171A3 (en)
MX (1) MXPA02000842A (en)
WO (1) WO2001007484A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835556B2 (en) 2002-12-31 2014-09-16 Nektar Therapeutics Hydrolytically stable maleimide-terminated polymers

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EP1530631B1 (en) * 2002-01-16 2013-08-07 Genencor International, Inc. Multiply-substituted protease variants
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
MXPA05007151A (en) * 2002-12-31 2005-09-21 Nektar Therapeutics Al Corp Hydrolytically stable maleimide-terminated polymers.
GB0416155D0 (en) * 2004-07-20 2004-08-18 Unilever Plc Laundry product
GB0423986D0 (en) * 2004-10-29 2004-12-01 Unilever Plc Method of preparing a laundry product
TWI415628B (en) 2006-02-28 2013-11-21 Avon Prod Inc Compositions containing peptides with non-natural amino acids and methods of use
GB0610801D0 (en) 2006-05-31 2006-07-12 Unilever Plc Laundry product
US9828597B2 (en) * 2006-11-22 2017-11-28 Toyota Motor Engineering & Manufacturing North America, Inc. Biofunctional materials
JP2014091815A (en) * 2012-11-06 2014-05-19 Kao Corp Detergent composition
JP2017149952A (en) * 2017-02-28 2017-08-31 花王株式会社 Detergent composition

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835556B2 (en) 2002-12-31 2014-09-16 Nektar Therapeutics Hydrolytically stable maleimide-terminated polymers

Also Published As

Publication number Publication date
CA2379729A1 (en) 2001-02-01
WO2001007484A2 (en) 2001-02-01
AU5928400A (en) 2001-02-13
EP1196548A2 (en) 2002-04-17
JP2003516116A (en) 2003-05-13
BR0012694A (en) 2002-04-09
CZ2002171A3 (en) 2002-06-12
US6566115B1 (en) 2003-05-20
KR20020021395A (en) 2002-03-20
CN1377406A (en) 2002-10-30
MXPA02000842A (en) 2002-07-30

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