WO2001016369A3 - Uv excitable fluorescent energy transfer dyes - Google Patents

Uv excitable fluorescent energy transfer dyes Download PDF

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Publication number
WO2001016369A3
WO2001016369A3 PCT/US2000/021519 US0021519W WO0116369A3 WO 2001016369 A3 WO2001016369 A3 WO 2001016369A3 US 0021519 W US0021519 W US 0021519W WO 0116369 A3 WO0116369 A3 WO 0116369A3
Authority
WO
WIPO (PCT)
Prior art keywords
energy transfer
dye
transfer dyes
dyes
fluorescent energy
Prior art date
Application number
PCT/US2000/021519
Other languages
French (fr)
Other versions
WO2001016369A2 (en
Inventor
Linda G Lee
Original Assignee
Pe Corp Ny
Linda G Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pe Corp Ny, Linda G Lee filed Critical Pe Corp Ny
Priority to EP00950997A priority Critical patent/EP1212457B1/en
Priority to JP2001520914A priority patent/JP2003508065A/en
Priority to DE60042163T priority patent/DE60042163D1/en
Priority to CA002382663A priority patent/CA2382663A1/en
Priority to AU64010/00A priority patent/AU6401000A/en
Priority to AT00950997T priority patent/ATE430811T1/en
Publication of WO2001016369A2 publication Critical patent/WO2001016369A2/en
Publication of WO2001016369A3 publication Critical patent/WO2001016369A3/en
Priority to AU2006200848A priority patent/AU2006200848A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

Novel energy transfer dyes which can be used with shorter wavelength light sources are provided. These dyes include a donor dye with an absorption maxima at a wavelength between about 250 to 450 nm and an acceptor dye which is capable of absorbing energy emitted from the donor dye. One of the energy transfer dyes has a donor dye which is a member of a class of dyes having a coumarin or pyrene ring structure and an acceptor dye which is capable of absorbing energy emitted from the donor dye, wherein the donor dye has an absorption maxima between about 250 and 450 nm and the acceptor dye has an emission maxima at a wavelength greater than about 500 nm.
PCT/US2000/021519 1999-08-27 2000-08-04 Uv excitable fluorescent energy transfer dyes WO2001016369A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP00950997A EP1212457B1 (en) 1999-08-27 2000-08-04 Uv excitable fluorescent energy transfer dyes
JP2001520914A JP2003508065A (en) 1999-08-27 2000-08-04 UV-excitable fluorescent energy transfer dye
DE60042163T DE60042163D1 (en) 1999-08-27 2000-08-04 UV FLUORESCENT ADJUSTABLE FLUORESCENCE TRANSFER DYES
CA002382663A CA2382663A1 (en) 1999-08-27 2000-08-04 Uv excitable fluorescent energy transfer dyes
AU64010/00A AU6401000A (en) 1999-08-27 2000-08-04 Uv excitable fluorescent energy transfer dyes
AT00950997T ATE430811T1 (en) 1999-08-27 2000-08-04 FLUORESCENCE ENERGY TRANSFER DYES EXCITED WITH UV LIGHT
AU2006200848A AU2006200848A1 (en) 1999-08-27 2006-02-28 UV excitable fluorescent energy transfer dyes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/385,352 US6358684B1 (en) 1999-08-27 1999-08-27 UV excitable fluorescent energy transfer dyes
US09/385,352 1999-08-27

Publications (2)

Publication Number Publication Date
WO2001016369A2 WO2001016369A2 (en) 2001-03-08
WO2001016369A3 true WO2001016369A3 (en) 2001-10-04

Family

ID=23521062

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/021519 WO2001016369A2 (en) 1999-08-27 2000-08-04 Uv excitable fluorescent energy transfer dyes

Country Status (8)

Country Link
US (11) US6358684B1 (en)
EP (1) EP1212457B1 (en)
JP (1) JP2003508065A (en)
AT (1) ATE430811T1 (en)
AU (2) AU6401000A (en)
CA (1) CA2382663A1 (en)
DE (1) DE60042163D1 (en)
WO (1) WO2001016369A2 (en)

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Also Published As

Publication number Publication date
AU2006200848A1 (en) 2006-03-23
EP1212457A2 (en) 2002-06-12
US7378517B2 (en) 2008-05-27
US20090031506A1 (en) 2009-02-05
US7402671B2 (en) 2008-07-22
EP1212457B1 (en) 2009-05-06
US8129529B2 (en) 2012-03-06
US8604186B2 (en) 2013-12-10
CA2382663A1 (en) 2001-03-08
ATE430811T1 (en) 2009-05-15
US6358684B1 (en) 2002-03-19
US20020058272A1 (en) 2002-05-16
US20010049109A1 (en) 2001-12-06
AU6401000A (en) 2001-03-26
US20030165961A1 (en) 2003-09-04
US7012141B2 (en) 2006-03-14
US20060155123A1 (en) 2006-07-13
WO2001016369A2 (en) 2001-03-08
US20060269958A1 (en) 2006-11-30
DE60042163D1 (en) 2009-06-18
US20060029936A9 (en) 2006-02-09
US20040076971A1 (en) 2004-04-22
US20110077415A1 (en) 2011-03-31
US20120196295A1 (en) 2012-08-02
JP2003508065A (en) 2003-03-04
US7888507B2 (en) 2011-02-15
US20090062543A1 (en) 2009-03-05
US7157572B2 (en) 2007-01-02

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