WO2001036663A9 - Bio-burden visualization system - Google Patents
Bio-burden visualization systemInfo
- Publication number
- WO2001036663A9 WO2001036663A9 PCT/US2000/042153 US0042153W WO0136663A9 WO 2001036663 A9 WO2001036663 A9 WO 2001036663A9 US 0042153 W US0042153 W US 0042153W WO 0136663 A9 WO0136663 A9 WO 0136663A9
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- instrument
- ultraviolet light
- dye
- viewing
- medical instrument
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6447—Fluorescence; Phosphorescence by visual observation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
Definitions
- the present invention relates to a bio-burden visualization system, and more in particular, to a system and method for visualizing organic bio-burden residues on medical instruments.
- bio-burden visualization system which is relatively simple to use and highly effective in allowing visualization of bio-burden remaining on surgical instruments .
- Yet another object of the present invention is to provide a bio-burden visualization system which is reliable in use.
- a further object of the present invention is to provide a bio-burden visualization system which is economical and yet has a high degree of accuracy.
- a bio-burden visualization system and method which utilizes visualizing compounds or dyes that will affix to specific bio-burden residues and fluoresce or phosphoresce under appropriate conditions.
- These visualization compounds are generally in a liquid form which can be easily applied to an instrument by dipping or swabbing. However, solid or gas visualizing compounds may also be used.
- the method of the present invention can be utilized to visualize bio-burden residues left on surfaces of surgical instruments, medical equipment and the like after cleaning or after sterilization.
- the system can also be used in a training program to test the quality of cleaning supplies and techniques used in the cleaning process. It could also be used for inspection, training and certification of cleanliness on food preparation surfaces.
- Figure 1 is a perspective view of a device for use in the bio-burden visualization system and method of the present invention
- Figure 2 is a perspective view of a technique for applying a visualization dye to a surgical instrument for use in the present invention
- Figure 3 is a perspective view of a mounting block for surgical instruments to be supported within the apparatus of Figure 1 ;
- Figure 4 is a sectional view taken along line 4-4 of Figure 1 ;
- Figure 5 is a photograph of an unclean surgical instrument viewed using the process of the invention.
- Figure 6 is a photograph of the same instrument afer cleaning when viewed with the system of the present invention.
- Figure 7 is an instrument after cleaning when viewed with the system of the present invention with visualized bio-burden.
- a viewing cabinet or housing 10 is illustrated in which visualization of bio-burden takes place according to the present invention.
- the housing consists of an enclosure having an open front end 12 which is closed by a flexible light shield 14 or the like, which allows access to the interior of the housing.
- the light shield as illustrated is a flexible sheet of black material, but could be a door or the like.
- a camera support which is in the shape of frustro- pyramid 16 is mounted above an opening on the top of the housing and provides support at its upper end 18 for a camera 20.
- the camera may be a conventional photographic film camera or an electronic CCD camera of conventional construction.
- the upper surface 22 of housing 10 has ultraviolet light sources 24 mounted thereon, which project ultraviolet light through openings in top wall 22 to the interior of the housing.
- visualization chamber 10 is illustrated in greater detail in Figure 4. As seen therein the housing has openings 26 therein above which the ultraviolet fixtures 24 are mounted in any convenient manner.
- Support 16 is mounted above an opening 28 in the housing in any convenient manner. Preferably the support 16 simply sits about the periphery of the opening.
- the ultraviolet light fixtures 24 project light into the interior of the chamber towards a removable instrument mounting block 32.
- the mounting block may be formed in any convenient manner, for example, it may simply be a styrofoam block 32, as illustrated in
- FIG 3 having a plurality of slots 33 formed therein which will frictionally retain a surgical instrument such as a scalpel, clamp, scissors or the like therein.
- a surgical instrument such as a scalpel, clamp, scissors or the like.
- other convenient mounting systems could also be used.
- the present invention is intended for use in checking for the presence of, i.e., visualizing, bio-burden on a surgical instrument, whether before or after sterilization.
- a visualizing compound which can be one of a number of fluorescent substances, or combination of substances, that have the ability to fluoresce or phosphoresce when attached to a organic residue and subjected to ultraviolet light.
- a surgical instrument 40 is illustrated in Figure 2, as having an appropriate dye applied to it by a swab.
- the swab is used once so as not to contaminate other instruments.
- Alternative methods of applying the dye may also be used such as dipping, immersion, or spray.
- the instrument is mounted in block 32 in chamber 10 immediately beneath the camera 20.
- Ultraviolet lights 24 are then turned on and the instrument can be viewed through the camera or, if the camera is a CCD instrument, through a computer terminal 42 connected to the camera using known software. If there is bio-burden on the instrument, it will fluoresce and appear to the viewer, although the bio-burden would not necessarily visually be seen under normal light.
- a third ultraviolet light source 24 can be mounted within chamber 10 to project light horizontally in the chamber to further illuminate the instrument.
- visualization chamber 10 may be a chamber such as the Chromata View viewing cabinet available under Model No. C-65 from
- the light sources may be conventional ultraviolet light fixtures which sit on the cabinet, and which produce ultraviolet light in the range of between 254nm to 365nm.
- the camera uses a 90mm macro-lens in order to enable the viewer to obtain a one-to-one size image which is clearer and has a larger depth of field.
- Figures 5 and 6 show the results of the process, wherein Figure 5 shows a fluoresced surgical instrument carrying bio-burden and the bio-burden is shown as fluorescing on the instrument. Figure 6 shows the same instrument after proper cleaning with no fluorescent material visible.
- the system and method of the present invention could be used to visualize organic residues left on surgical and medical instruments after cleaning; or after sterilization, or in a training program to insure proper cleaning techniques or to check the efficacy of cleaning supplies.
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU30818/01A AU3081801A (en) | 1999-11-15 | 2000-11-15 | Bio-burden visualization system |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16560599P | 1999-11-15 | 1999-11-15 | |
US60/165,605 | 1999-11-15 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2001036663A2 WO2001036663A2 (en) | 2001-05-25 |
WO2001036663A3 WO2001036663A3 (en) | 2002-03-14 |
WO2001036663A9 true WO2001036663A9 (en) | 2002-08-29 |
Family
ID=22599623
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2000/042153 WO2001036663A2 (en) | 1999-11-15 | 2000-11-15 | Bio-burden visualization system |
Country Status (3)
Country | Link |
---|---|
US (1) | US6653146B1 (en) |
AU (1) | AU3081801A (en) |
WO (1) | WO2001036663A2 (en) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101145508B1 (en) | 2003-06-18 | 2012-05-15 | 사이토리 테라퓨틱스, 인크. | Methods of using adipose tissue-derived cells in augmenting autologous fat transfer |
BRPI0411798A (en) | 2003-06-25 | 2006-08-08 | Macropore Biosurgery Inc | systems and methods for separating and concentrating regenerative cells from tissue |
MXPA06003088A (en) | 2003-09-17 | 2006-06-20 | Macropore Biosurgery Inc | Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders. |
ES2602559T3 (en) | 2004-03-19 | 2017-02-21 | Cytori Therapeutics, Inc. | Cellular support and cell support containment devices containing regenerative cells |
WO2006014159A2 (en) | 2004-07-01 | 2006-02-09 | Macropore Biosurgery Inc. | Methods of using regenerative cells in the treatment of musculoskeletal disorders |
PL1778833T3 (en) | 2004-07-01 | 2011-10-31 | Cytori Therapeutics Inc | Methods of using regenerative cells to promote wound healing |
AU2005332046B2 (en) | 2005-05-25 | 2013-05-16 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions |
CN101006343B (en) * | 2005-06-30 | 2012-10-31 | 阿姆泰克株式会社 | Indication composition for surgical instrument cleaning evaluation |
US20070082034A1 (en) * | 2005-10-11 | 2007-04-12 | Dragani Patrick B | Dual-sided cleansing wipes for medical equipment |
WO2007054238A1 (en) * | 2005-11-08 | 2007-05-18 | Unilever N.V. | Hygiene indicator device |
DE202007017612U1 (en) * | 2007-12-14 | 2008-03-27 | Sinepro Gmbh | Test soiling for testing the cleaning success of instruments in washer-disinfectors |
GB201014016D0 (en) * | 2010-08-20 | 2010-10-06 | Synoptics Ltd | Imaging system and associated method for detection of protein contamination |
DE102011054448A1 (en) * | 2011-10-13 | 2013-04-18 | How To Organize (H2O) Gmbh | Apparatus and method for detecting anomalies on instruments |
US10705020B2 (en) | 2013-02-26 | 2020-07-07 | Steris Inc. | Method and apparatus for optical detection of bio-contaminants within a lumen |
US9354182B2 (en) * | 2013-02-26 | 2016-05-31 | Steris Inc. | Method for optical detection of bio-contaminants |
GB2542341B (en) | 2015-09-11 | 2019-12-25 | Kumar Kanhye Yogesh | Method and apparatus for pathogen testing |
Family Cites Families (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4094745A (en) | 1973-06-22 | 1978-06-13 | John Scholefield | Method of staining microscopic organisms |
US3883247A (en) | 1973-10-30 | 1975-05-13 | Bio Physics Systems Inc | Method for fluorescence analysis of white blood cells |
US4087685A (en) | 1977-01-11 | 1978-05-02 | International Business Machines Corporation | Fluorescent microanalytical system and method for detecting and identifying organic materials |
US4115699A (en) | 1977-06-16 | 1978-09-19 | Kabushiki Kaisha Nihon Kotai Kenkyujo | Apparatus for sensitive detection and quantitative analysis of biological and biochemical substances |
US4390787A (en) | 1980-10-29 | 1983-06-28 | The United States Of America As Represented By The Secretary Of Agriculture | Method and apparatus for automatic egg mass counting |
GB2097144A (en) | 1981-03-23 | 1982-10-27 | Tno | New method for identifying algae in water samples and apparatus for use in that method |
SE425439B (en) | 1981-04-30 | 1982-09-27 | Wallac Oy | IMMUNOLOGICAL ANALYSIS WITH LANTANIDE CHELAT COMPLEX AS MARKOR |
US4621193A (en) * | 1984-11-21 | 1986-11-04 | Michael Van Hoye | Fluorescent penetrant crack detection |
JPS61164158A (en) * | 1985-01-16 | 1986-07-24 | Aruboosu Yakushiyou Kk | Composition for inspection of protein |
US4800282A (en) | 1985-02-07 | 1989-01-24 | Sharp Kabushiki Kaisha | Apparatus and method for detecting residual organic compounds |
JPS61186854A (en) | 1985-02-14 | 1986-08-20 | Fuji Photo Film Co Ltd | Instrument for measuring number of bacteria in ultra-pure water |
US4812393A (en) | 1986-01-31 | 1989-03-14 | Eastman Kodak Company | Fluorescent dyes and biological and analytical uses thereof |
US4858465A (en) * | 1988-06-21 | 1989-08-22 | Rockwell International Corporation | Water washable contaminant detection and labeling compositions and method for utilizing same |
US4866283A (en) | 1988-08-12 | 1989-09-12 | Southwest Research Institute | Optical inspection of food products |
US4938224A (en) | 1988-11-16 | 1990-07-03 | Rysavy Joseph A | Process for detecting accidental contact with body fluids |
DK239589D0 (en) * | 1989-05-17 | 1989-05-17 | Dst Kemi A S | PROCEDURE FOR SIMULTAN CLEANING AND HYGIENE CONTROL AND METHOD FOR USING THE PROCEDURE |
CA2131618A1 (en) | 1992-03-20 | 1993-09-30 | Kenneth Leslie Rabone | Improvements in or relating to cleaning compositions |
US5474910A (en) | 1993-10-15 | 1995-12-12 | Alfano; Robert R. | Method and device for detecting biological molecules and/or microorganisms within a desired area or space |
US5624810A (en) | 1995-01-09 | 1997-04-29 | New Horizons Diagnostics Corp. | Method for detection of surfaces contaminants |
US5900067A (en) * | 1995-05-16 | 1999-05-04 | Jones; C. Kerry | Handwashing technique analysis |
DE19602673A1 (en) * | 1996-01-25 | 1997-08-07 | Pereg Gmbh | Synthetic test soiling |
US5667840A (en) | 1996-02-27 | 1997-09-16 | Becton Dickinson And Company | Lubricant soluble fluorescent agent and method for its use in a system for detection of lubricant coatings |
US5701012A (en) | 1996-03-19 | 1997-12-23 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Fluorescent biological particle detection system |
US5827740A (en) * | 1996-07-30 | 1998-10-27 | Osiris Therapeutics, Inc. | Adipogenic differentiation of human mesenchymal stem cells |
DE19631668C2 (en) * | 1996-08-06 | 1998-07-02 | Univ Eberhard Karls | Methods for determining the cleaning action on cleaned objects, methods for determining the contamination of objects to be cleaned and use of these methods |
DE19633808A1 (en) * | 1996-08-22 | 1998-02-26 | Markus Dr Spiekermann | Detecting microbial contamination |
WO1998021569A1 (en) * | 1996-11-13 | 1998-05-22 | Bruno Pregenzer | Process for detecting whether a surface has been treated with a detergent and/or disinfectant |
DE19649925A1 (en) * | 1996-12-02 | 1998-06-04 | Intermedical S A H | Quantifying cleaning, disinfection or sterilisation of articles |
US5863790A (en) | 1997-05-14 | 1999-01-26 | Minnesota Mining And Manfacturing Company | Biological sterility indicator |
EP0913681A3 (en) | 1997-11-03 | 1999-11-10 | Karin Bartling-Dudziak | Device and set for visual recognition and/or for displaying the distribution of fluorescent cosmetic or dermatological compositions and composition for topical application |
DE29719497U1 (en) * | 1997-11-03 | 1998-01-08 | Bartling Dudziak Karin | Device for visually recognizing and / or displaying the distribution of fluorescent cosmetic or dermatological preparations |
JP3109042B2 (en) * | 1998-03-30 | 2000-11-13 | 株式会社イナックス | Visualization judgment method of soil containing protein of plumbing products |
-
2000
- 2000-11-15 US US09/713,666 patent/US6653146B1/en not_active Expired - Lifetime
- 2000-11-15 WO PCT/US2000/042153 patent/WO2001036663A2/en active Application Filing
- 2000-11-15 AU AU30818/01A patent/AU3081801A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US6653146B1 (en) | 2003-11-25 |
WO2001036663A2 (en) | 2001-05-25 |
AU3081801A (en) | 2001-05-30 |
WO2001036663A3 (en) | 2002-03-14 |
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