WO2001046467A3 - Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators - Google Patents

Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators Download PDF

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Publication number
WO2001046467A3
WO2001046467A3 PCT/IB2000/001930 IB0001930W WO0146467A3 WO 2001046467 A3 WO2001046467 A3 WO 2001046467A3 IB 0001930 W IB0001930 W IB 0001930W WO 0146467 A3 WO0146467 A3 WO 0146467A3
Authority
WO
WIPO (PCT)
Prior art keywords
hybridization
assay
nucleic acid
duplex
fluorescent
Prior art date
Application number
PCT/IB2000/001930
Other languages
French (fr)
Other versions
WO2001046467A2 (en
Inventor
Jasmine I Daksis
Pierre Picard
Glen H Erikson
Original Assignee
Ingeneus Corp
Jasmine I Daksis
Pierre Picard
Glen H Erikson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU17263/01A priority Critical patent/AU778892B2/en
Application filed by Ingeneus Corp, Jasmine I Daksis, Pierre Picard, Glen H Erikson filed Critical Ingeneus Corp
Priority to EP00979887A priority patent/EP1242620B1/en
Priority to DE60036307T priority patent/DE60036307T2/en
Priority to BRPI0016614A priority patent/BR0016614A8/en
Priority to MXPA02006262A priority patent/MXPA02006262A/en
Priority to CA2394752A priority patent/CA2394752C/en
Priority to IL15028400A priority patent/IL150284A0/en
Priority to JP2001546963A priority patent/JP4041867B2/en
Publication of WO2001046467A2 publication Critical patent/WO2001046467A2/en
Publication of WO2001046467A3 publication Critical patent/WO2001046467A3/en
Priority to IL150284A priority patent/IL150284A/en
Priority to KR20027008065A priority patent/KR100537979B1/en
Priority to HK02108903.7A priority patent/HK1047454B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays

Abstract

The invention provides a homogeneous assay for nucleic acid hybridization. The fluorescent intensity of a hybridization medium containing a probe, a target and an intercalating agent is a function of the hybridization efficiency of the probe with respect to the target. The assay can detect specific hybridization between single-stranded probes and non-denatured double-stranded targets to form triplexes, thus obviating the need to denature the targets. The assay can also detect duplex hybridization complexes. The assay can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.
PCT/IB2000/001930 1999-12-21 2000-12-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators WO2001046467A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CA2394752A CA2394752C (en) 1999-12-21 2000-12-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators
EP00979887A EP1242620B1 (en) 1999-12-21 2000-12-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators
DE60036307T DE60036307T2 (en) 1999-12-21 2000-12-21 FLUORESCENCE INTENSITY TEST FOR DUPLEX AND TRIPLEX NUCLEIC ACID HYBRIDIZATION IN SOLUTION USING FLUORESCENCE INTERCALATORS
BRPI0016614A BR0016614A8 (en) 1999-12-21 2000-12-21 Fluorescence intensity assay for duplex and triplex nucleic acid hybridization in solution using fluorescent intercalators
MXPA02006262A MXPA02006262A (en) 1999-12-21 2000-12-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators.
AU17263/01A AU778892B2 (en) 1999-12-21 2000-12-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators
IL15028400A IL150284A0 (en) 1999-12-21 2000-12-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators
JP2001546963A JP4041867B2 (en) 1999-12-21 2000-12-21 Fluorescence intensity measurement method for double-stranded and triple-stranded nucleic acid hybridization in solution using fluorescent intercalator
IL150284A IL150284A (en) 1999-12-21 2002-06-18 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators
KR20027008065A KR100537979B1 (en) 1999-12-21 2002-06-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators
HK02108903.7A HK1047454B (en) 1999-12-21 2002-12-06 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/468,679 US6403313B1 (en) 1999-12-21 1999-12-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US09/468,679 1999-12-21

Publications (2)

Publication Number Publication Date
WO2001046467A2 WO2001046467A2 (en) 2001-06-28
WO2001046467A3 true WO2001046467A3 (en) 2002-05-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/001930 WO2001046467A2 (en) 1999-12-21 2000-12-21 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization in solution utilizing fluorescent intercalators

Country Status (17)

Country Link
US (1) US6403313B1 (en)
EP (1) EP1242620B1 (en)
JP (1) JP4041867B2 (en)
KR (1) KR100537979B1 (en)
CN (1) CN1413264A (en)
AT (1) ATE372390T1 (en)
AU (1) AU778892B2 (en)
BR (1) BR0016614A8 (en)
CA (1) CA2394752C (en)
DE (1) DE60036307T2 (en)
ES (1) ES2296661T3 (en)
HK (1) HK1047454B (en)
IL (2) IL150284A0 (en)
MX (1) MXPA02006262A (en)
PT (1) PT1242620E (en)
RU (1) RU2273668C2 (en)
WO (1) WO2001046467A2 (en)

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Also Published As

Publication number Publication date
US6403313B1 (en) 2002-06-11
JP4041867B2 (en) 2008-02-06
MXPA02006262A (en) 2004-09-06
BR0016614A (en) 2003-01-07
ES2296661T3 (en) 2008-05-01
DE60036307T2 (en) 2008-05-29
RU2273668C2 (en) 2006-04-10
HK1047454A1 (en) 2003-02-21
IL150284A0 (en) 2002-12-01
ATE372390T1 (en) 2007-09-15
AU1726301A (en) 2001-07-03
KR100537979B1 (en) 2005-12-21
JP2003517841A (en) 2003-06-03
EP1242620B1 (en) 2007-09-05
WO2001046467A2 (en) 2001-06-28
CA2394752C (en) 2012-09-25
DE60036307D1 (en) 2007-10-18
HK1047454B (en) 2008-07-25
BR0016614A8 (en) 2019-01-22
RU2002119394A (en) 2004-01-27
CA2394752A1 (en) 2001-06-28
CN1413264A (en) 2003-04-23
IL150284A (en) 2008-03-20
PT1242620E (en) 2007-12-13
EP1242620A2 (en) 2002-09-25
AU778892B2 (en) 2004-12-23
KR20020079757A (en) 2002-10-19

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