WO2001051618A3 - Purification of microglial cells - Google Patents

Purification of microglial cells Download PDF

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Publication number
WO2001051618A3
WO2001051618A3 PCT/US2001/000362 US0100362W WO0151618A3 WO 2001051618 A3 WO2001051618 A3 WO 2001051618A3 US 0100362 W US0100362 W US 0100362W WO 0151618 A3 WO0151618 A3 WO 0151618A3
Authority
WO
WIPO (PCT)
Prior art keywords
microglial cells
purification
cells
cell population
immunoglobulin
Prior art date
Application number
PCT/US2001/000362
Other languages
French (fr)
Other versions
WO2001051618A2 (en
Inventor
Huaiyu Mi
Saili Yi
Original Assignee
Agy Therapeutics Inc
Huaiyu Mi
Saili Yi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agy Therapeutics Inc, Huaiyu Mi, Saili Yi filed Critical Agy Therapeutics Inc
Priority to AU27650/01A priority Critical patent/AU2765001A/en
Publication of WO2001051618A2 publication Critical patent/WO2001051618A2/en
Publication of WO2001051618A3 publication Critical patent/WO2001051618A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier

Abstract

The invention provides a method for obtaining a cell population enriched in microglial cells by contacting a composition comprising microglial cells with immunoglobulin immobilized on a matrix, allowing the microglial cells to bind the immobilized immunoglobulin; and, removing non-adherent cells. A cell population enriched in microglial cells is produced and may be cultured.
PCT/US2001/000362 2000-01-07 2001-01-05 Purification of microglial cells WO2001051618A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU27650/01A AU2765001A (en) 2000-01-07 2001-01-05 Purification of microglial cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/479,589 US6586239B1 (en) 2000-01-07 2000-01-07 Purifying microglial cells by binding cell Fc receptor to immunoglobulin G Fc domain
US09/479,589 2000-01-07

Publications (2)

Publication Number Publication Date
WO2001051618A2 WO2001051618A2 (en) 2001-07-19
WO2001051618A3 true WO2001051618A3 (en) 2002-03-07

Family

ID=23904615

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/000362 WO2001051618A2 (en) 2000-01-07 2001-01-05 Purification of microglial cells

Country Status (3)

Country Link
US (1) US6586239B1 (en)
AU (1) AU2765001A (en)
WO (1) WO2001051618A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003014151A2 (en) 2001-08-10 2003-02-20 Genset S.A. Human secreted proteins, their encoding polynucleotides, and uses thereof
TW202237652A (en) * 2020-11-30 2022-10-01 醣基生醫股份有限公司 Antibody for enrichment of cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0415801A1 (en) * 1989-07-05 1991-03-06 N.V. Innogenetics S.A. Monoclonal antibodies against activated microglial cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981194A (en) * 1992-07-10 1999-11-09 University Of British Columbia Use of p97 and iron binding proteins as diagnostic and therapeutic agents

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0415801A1 (en) * 1989-07-05 1991-03-06 N.V. Innogenetics S.A. Monoclonal antibodies against activated microglial cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FONG S ET AL: "FRACTIONATION OF HUMAN LYMPHOCYTE SUB POPULATIONS ON IMMUNO GLOBULIN COATED PETRI DISHES", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 44, no. 2, 1981, pages 171 - 182, XP000926557, ISSN: 0022-1759 *
GIULIAN D ET AL: "CHARACTERIZATION OF AMOEBOID MICROGLIA ISOLATED FROM DEVELOPING MAMMALIAN BRAIN", JOURNAL OF NEUROSCIENCE, vol. 6, no. 8, 1986, pages 2163 - 2178, XP000926555, ISSN: 0270-6474 *
VEDELER CHRISTIAN ET AL: "Fc receptor for IgG (FcR) on rat microglia.", JOURNAL OF NEUROIMMUNOLOGY, vol. 49, no. 1-2, 1994, pages 19 - 24, XP000926556, ISSN: 0165-5728 *
WALKER DG ET AL.: "Complement and cytokine gene expression in cultured microglial derived from postmortem human brains", JOURNAL OF NEUROSCIENCE RESEARCH, vol. 40, no. 4, 1995, US, pages 478 - 493, XP000926558 *

Also Published As

Publication number Publication date
US6586239B1 (en) 2003-07-01
WO2001051618A2 (en) 2001-07-19
AU2765001A (en) 2001-07-24

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