WO2001059450A8 - Cells expressing zinc finger protein for drug discovery - Google Patents

Cells expressing zinc finger protein for drug discovery

Info

Publication number
WO2001059450A8
WO2001059450A8 PCT/US2001/004301 US0104301W WO0159450A8 WO 2001059450 A8 WO2001059450 A8 WO 2001059450A8 US 0104301 W US0104301 W US 0104301W WO 0159450 A8 WO0159450 A8 WO 0159450A8
Authority
WO
WIPO (PCT)
Prior art keywords
zinc finger
finger protein
cells expressing
drug discovery
cellular
Prior art date
Application number
PCT/US2001/004301
Other languages
French (fr)
Other versions
WO2001059450A2 (en
WO2001059450A3 (en
Inventor
Casey Case
Original Assignee
Sangamo Biosciences Inc
Casey Case
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA2398590A priority Critical patent/CA2398590C/en
Priority to AU2001250774A priority patent/AU2001250774B2/en
Priority to JP2001558729A priority patent/JP5047437B2/en
Priority to KR1020027010228A priority patent/KR20020086508A/en
Priority to EP01924089A priority patent/EP1254369B1/en
Priority to AU5077401A priority patent/AU5077401A/en
Application filed by Sangamo Biosciences Inc, Casey Case filed Critical Sangamo Biosciences Inc
Priority to AT01924089T priority patent/ATE483970T1/en
Priority to DE60143192T priority patent/DE60143192D1/en
Publication of WO2001059450A2 publication Critical patent/WO2001059450A2/en
Publication of WO2001059450A8 publication Critical patent/WO2001059450A8/en
Publication of WO2001059450A3 publication Critical patent/WO2001059450A3/en
Priority to HK03103204.3A priority patent/HK1051067A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Abstract

Disclosed herein are compositions and methods useful in screening a compound for its interaction and/or effect with a molecular target and/or cellular process. The invention is based on the construction of fusion proteins between a zinc finger protein designed to recognize a specific promoter and a transcription repressor or activator. Claimed are methods for the screening of substances capable of interacting with a specific cellular target or modulating a cellular pathway, involving cells stably transfected with, and expressing, the fusion protein.
PCT/US2001/004301 2000-02-08 2001-02-08 Cells expressing zinc finger protein for drug discovery WO2001059450A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
AU2001250774A AU2001250774B2 (en) 2000-02-08 2001-02-08 Cells expressing zinc finger proteins for drug discovery
JP2001558729A JP5047437B2 (en) 2000-02-08 2001-02-08 Cells for drug discovery
KR1020027010228A KR20020086508A (en) 2000-02-08 2001-02-08 Cells for drug discovery
EP01924089A EP1254369B1 (en) 2000-02-08 2001-02-08 Cells for drug discovery
AU5077401A AU5077401A (en) 2000-02-08 2001-02-08 Cells for drug discovery
CA2398590A CA2398590C (en) 2000-02-08 2001-02-08 Cells for drug discovery
AT01924089T ATE483970T1 (en) 2000-02-08 2001-02-08 CELLS FOR DRUG DISCOVERY
DE60143192T DE60143192D1 (en) 2000-02-08 2001-02-08 CELLS FOR THE DISCOVERY OF MEDICAMENTS
HK03103204.3A HK1051067A1 (en) 2000-02-08 2003-05-06 Cells for drug discovery

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18111700P 2000-02-08 2000-02-08
US60/181,117 2000-02-08

Publications (3)

Publication Number Publication Date
WO2001059450A2 WO2001059450A2 (en) 2001-08-16
WO2001059450A8 true WO2001059450A8 (en) 2001-10-18
WO2001059450A3 WO2001059450A3 (en) 2002-05-02

Family

ID=22662967

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/004301 WO2001059450A2 (en) 2000-02-08 2001-02-08 Cells expressing zinc finger protein for drug discovery

Country Status (10)

Country Link
US (3) US6689558B2 (en)
EP (2) EP1254369B1 (en)
JP (3) JP5047437B2 (en)
KR (1) KR20020086508A (en)
AT (1) ATE483970T1 (en)
AU (2) AU2001250774B2 (en)
CA (1) CA2398590C (en)
DE (1) DE60143192D1 (en)
HK (1) HK1051067A1 (en)
WO (1) WO2001059450A2 (en)

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