WO2001068834B1 - Detecteur de nucleotides, procede de production dudit detecteur, et procede de formation d'une membrane de particules fines - Google Patents

Detecteur de nucleotides, procede de production dudit detecteur, et procede de formation d'une membrane de particules fines

Info

Publication number
WO2001068834B1
WO2001068834B1 PCT/JP2001/002083 JP0102083W WO0168834B1 WO 2001068834 B1 WO2001068834 B1 WO 2001068834B1 JP 0102083 W JP0102083 W JP 0102083W WO 0168834 B1 WO0168834 B1 WO 0168834B1
Authority
WO
WIPO (PCT)
Prior art keywords
producing
fine particle
particle film
liquid
nucleotide
Prior art date
Application number
PCT/JP2001/002083
Other languages
English (en)
French (fr)
Other versions
WO2001068834A1 (fr
Inventor
Ichiro Yamashita
Original Assignee
Matsushita Electric Ind Co Ltd
Ichiro Yamashita
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matsushita Electric Ind Co Ltd, Ichiro Yamashita filed Critical Matsushita Electric Ind Co Ltd
Priority to EP01912416A priority Critical patent/EP1186659A4/en
Priority to AU41164/01A priority patent/AU4116401A/en
Publication of WO2001068834A1 publication Critical patent/WO2001068834A1/ja
Publication of WO2001068834B1 publication Critical patent/WO2001068834B1/ja
Priority to US10/628,840 priority patent/US7129047B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Claims

補正書の請求の範囲 [2001年 8月 28曰 （28. 08. 01 ) 国際事務局受理：出願当初の請求の範囲 5は 補正された；出願当初の請求の範囲 1は取り下げられた；他の請求の範囲は変更なし。 (2頁） ]
1. (削除）
2. 金属粒子と上記金属粒子を保持するタンパク質分子とからなる複合体を基 板上に配列させる工程 （a) と、
上記タンパク質分子を除去する工程 （b) と、
上記工程 （b) で形成された基板上に残存している上記金属粒子に、 互いに共 役となる 2つのヌクレオチド分子のうち、 いずれか一方のヌクレオチド分子を結 合する工程 （c) と、
を含むヌクレオチド検出装置の製造方法。
3. 請求項 2のヌクレオチド検出装置の製造方法において、
上記タンパク質分子が、 Dp sタンパク質またはアポフェリチンであることを 特徴とするヌクレオチド検出装置の製造方法。
4. 請求項 2または 3のヌクレオチド検出装置の製造方法において、
上記ヌクレオチド分子は、 塩基配列の異なる複数種類のヌクレオチド分子であ ることを特徴とするヌクレオチド検出装置。
5. (補正後） 基板を、 上記基板の表面が容器に充填された微粒子を含む液体 の液面に交差するように設置する工程 （a) と、
上記液体の液面を、 上昇または下降させ、 上記基板の表面上に上記微粒子を 2 次元状に分散させた濡れ膜を形成する工程 （b) と、
を含む微粒子膜の作製方法。
6. 請求項 5の微粒子膜の作製方法において、
上記微粒子が、 直径 50 nm以下であることを特徴とする微粒子膜の作製方法。
7. 請求項 5または 6の微粒子膜の作製方法において、 摘正された 紙 (条約第 19条) 上記微粒子は、 タンパク質であることを特徴とする微粒子膜の作製方法。
8 . 請求項 7の微粒子膜の作製方法において、
上記夕ンパク質は、 無機材料を内包することを特徴とする微粒子膜の作製方法
9 . 請求項 7または 8の微粒子膜の作製方法において、
上記液体のタンパク質濃度は、 1 0 g/m l〜5 0 O m g/m lであること を特徴とする微粒子膜の作製方法。
1 0 . 請求項 7 - 9のうちいずれか 1つの微粒子膜の作製方法において、 上記液体は、 電解質を含むことを特徴とする微粒子膜の作製方法。
1 1 . 請求項 7— 1 0のうちいずれか 1つの微粒子膜の作製方法において、 上記液体の液面の上昇または低下速度はほぼ一定であり、 且つ、 1 0 mm/分 以下であることを特徴とする微粒子膜の作製方法。
1 2 . 請求項 7— 1 1のうちいずれか 1つの微粒子膜の作製方法において、 上記液体は、 重力の作用によって流出させられることを特徴とする微粒子膜の 作製方法。
1 3 . 請求項 7— 1 2のうちいずれか 1つの微粒子膜の作製方法において、 上記基板は、 表面に凹凸パターンを有することを特徴とする微粒子膜の作製方 法 o
補正された用紙 (条約第19条 )
PCT/JP2001/002083 2000-03-16 2001-03-15 Detecteur de nucleotides, procede de production dudit detecteur, et procede de formation d'une membrane de particules fines WO2001068834A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01912416A EP1186659A4 (en) 2000-03-16 2001-03-15 NUCLEOTIDE DETECTOR, METHOD FOR THE PRODUCTION THEREOF AND METHOD FOR PRODUCING A MEMBRANE FROM FINE PARTICLES
AU41164/01A AU4116401A (en) 2000-03-16 2001-03-15 Nucleotide detector, process for producing the same and process for forming fineparticle membrane
US10/628,840 US7129047B2 (en) 2000-03-16 2003-07-28 Method for manufacturing a nucleotide detector

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2000-73805 2000-03-16
JP2000073805 2000-03-16
JP2000-86116 2000-03-27
JP2000086116 2000-03-27

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09/979,438 A-371-Of-International US20030013096A1 (en) 2000-03-16 2001-03-15 Nucleotide detector, process for producing the same and process for morming forming fine particle membrane
US10/628,840 Division US7129047B2 (en) 2000-03-16 2003-07-28 Method for manufacturing a nucleotide detector

Publications (2)

Publication Number Publication Date
WO2001068834A1 WO2001068834A1 (fr) 2001-09-20
WO2001068834B1 true WO2001068834B1 (fr) 2001-11-01

Family

ID=26587665

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/002083 WO2001068834A1 (fr) 2000-03-16 2001-03-15 Detecteur de nucleotides, procede de production dudit detecteur, et procede de formation d'une membrane de particules fines

Country Status (4)

Country Link
US (2) US20030013096A1 (ja)
EP (1) EP1186659A4 (ja)
AU (1) AU4116401A (ja)
WO (1) WO2001068834A1 (ja)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6713173B2 (en) 1996-11-16 2004-03-30 Nanomagnetics Limited Magnetizable device
GB2319253A (en) 1996-11-16 1998-05-20 Eric Leigh Mayes Composition, for use in a device, comprising a magnetic layer of domain-separated magnetic particles
US6986942B1 (en) 1996-11-16 2006-01-17 Nanomagnetics Limited Microwave absorbing structure
US6815063B1 (en) 1996-11-16 2004-11-09 Nanomagnetics, Ltd. Magnetic fluid
US8399383B2 (en) 2000-05-04 2013-03-19 Yale University Protein chips for high throughput screening of protein activity
US7102656B2 (en) * 2002-05-21 2006-09-05 Northwestern University Electrostatically driven lithography
EP1624306A4 (en) * 2003-04-30 2008-12-24 Chengdu Kuachang Medical Ind L DEVICE CONTAINING THE NANOSTRUCTURES USED FOR SEPARATION OR ANALYSIS AND MANUFACTURE AND APPLICATION THEREOF
AT501110A1 (de) * 2003-09-16 2006-06-15 Upper Austrian Res Gmbh Arrays zur bindung von molekülen
EP1809569A4 (en) * 2004-11-12 2009-12-02 Seoul Nat Univ Ind Foundation METHOD FOR ORIENTING OR MOUNTING A NANOSTRUCTURE ONTO A SOLID SURFACE
JP5092405B2 (ja) * 2006-01-12 2012-12-05 東レ株式会社 選択結合性物質固定化担体
US20100233384A1 (en) * 2006-03-20 2010-09-16 Japan Science And Techonology Agency Immobilization of metal nanoparticles
JP5001019B2 (ja) * 2007-02-02 2012-08-15 株式会社日立ハイテクノロジーズ 生体分子検出素子、生体分子検出素子の製造方法及び生体分子検出方法
KR100891095B1 (ko) * 2007-02-13 2009-03-31 삼성전자주식회사 마이크로 어레이 및 그 제조 방법
PT103730A (pt) * 2007-05-04 2008-11-04 Univ Nova De Lisboa Método colorimétrico e estojo de detecção de sequências específicas de ácidos nucleicos através de nanopartículas metálicas funcionalizadas com oligonucleótidos modificados
US7871571B2 (en) * 2007-10-25 2011-01-18 Parker John A Biomolecule analyzing system
JP2009170280A (ja) * 2008-01-17 2009-07-30 Sony Corp 冷陰極電界電子放出素子の製造方法及び冷陰極電界電子放出表示装置の製造方法
CA2832672A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
CN103033546B (zh) * 2012-12-25 2014-10-29 南京工业大学 一种有机无机杂化材料修饰电极及其制备方法
EP3022400B1 (en) 2013-07-15 2023-06-21 Raytheon Technologies Corporation Turbine vanes with variable fillets
WO2018047771A1 (ja) * 2016-09-09 2018-03-15 国立大学法人大阪大学 核酸検出用素子、および核酸検出方法
CN114177964B (zh) * 2022-01-18 2023-01-31 军事科学院系统工程研究院卫勤保障技术研究所 一种平面单层微小液滴阵列的制备方法及其应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62183882A (ja) * 1986-02-07 1987-08-12 Fujitsu Ltd タンパク質累積膜の製造方法
US4779562A (en) 1986-03-19 1988-10-25 Fujitsu Limited Apparatus for depositing mono-molecular layer
JP2545363B2 (ja) * 1986-04-18 1996-10-16 富士通株式会社 基板上へのレジスト層形成方法
KR950002917B1 (ko) * 1989-08-18 1995-03-28 몬산토 캄파니 페리틴 유사체
US5232503A (en) * 1991-10-07 1993-08-03 San Jose Technology Apparatus for evaporatively coating objects with a predetermined thickness profile
JP2915812B2 (ja) 1994-12-07 1999-07-05 科学技術振興事業団 微粒子膜の転写付着方法
JP3756213B2 (ja) 1995-02-27 2006-03-15 独立行政法人科学技術振興機構 微粒子膜とその形成方法並びにその装置
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
JP2912562B2 (ja) 1995-02-27 1999-06-28 科学技術振興事業団 微粒子膜のサブミクロンリソグラフィー方法
AU4043497A (en) * 1996-07-29 1998-02-20 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
JP3649588B2 (ja) * 1997-05-30 2005-05-18 松下電器産業株式会社 量子ドットの製造方法
DE69825939T2 (de) * 1997-05-30 2005-09-15 Matsushita Electric Industrial Co., Ltd., Kadoma Anordnung mit Quanten-Schachteln
US6239255B1 (en) * 1997-08-29 2001-05-29 Clement E. Furlong Versatile surface plasmon resonance biosensors
JP3829491B2 (ja) 1998-08-27 2006-10-04 株式会社日立製作所 プローブチップ、プローブチップ作成方法、試料検出方法、及び試料検出装置
WO2000029617A2 (en) * 1998-09-24 2000-05-25 Advanced Research And Technology Institute, Inc. Water-soluble luminescent quantum dots and bioconjugates thereof
US5958701A (en) * 1999-01-27 1999-09-28 The United States Of America As Represented By The Secretary Of The Navy Method for measuring intramolecular forces by atomic force
CA2270807A1 (en) * 1999-05-03 2000-11-03 Ibm Canada Limited-Ibm Canada Limitee Closed chamber method and apparatus for the coating of liquid films
AU784040B2 (en) * 1999-06-25 2006-01-19 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
JP4403618B2 (ja) * 1999-12-21 2010-01-27 パナソニック株式会社 カーボンナノチューブの製造方法

Also Published As

Publication number Publication date
US20040018548A1 (en) 2004-01-29
US7129047B2 (en) 2006-10-31
WO2001068834A1 (fr) 2001-09-20
US20030013096A1 (en) 2003-01-16
EP1186659A4 (en) 2002-07-17
EP1186659A1 (en) 2002-03-13
AU4116401A (en) 2001-09-24

Similar Documents

Publication Publication Date Title
WO2001068834B1 (fr) Detecteur de nucleotides, procede de production dudit detecteur, et procede de formation d'une membrane de particules fines
Zaidi Molecular imprinting polymers and their composites: A promising material for diverse applications
US7270850B2 (en) Mesoporous permeation layers for use on active electronic matrix devices
Kinge et al. Self‐assembling nanoparticles at surfaces and interfaces
Dutta et al. Self-organization of colloidal nanoparticles
Bellah et al. Nanostructures for medical diagnostics
CA2516820A1 (en) Nanocylinder-modified surfaces
JP2011081001A (ja) 表面増強ラマン散乱を利用した生化学物質の検出方法
JP2001507441A (ja) 核酸および他の荷電された分子を移動させそして分離するための微小電気泳動チップ
ATE502117T1 (de) Nanopartikel mit gebundenen oligonukleotiden und deren verwendungen
TW419396B (en) Modification of surfaces to increase the surface tension
CN108414492A (zh) 利用自组装三维纳米结构为基底进行sers定量分析的方法
JP6462746B2 (ja) 帯電したナノ粒子の連続勾配を有する表面、接着現象の分析用の装置、ならびに表面および装置の使用
US20080293592A1 (en) Method For Covalently Immobilising Biomolecules on Organic Surfaces
TWI311155B (en) Direct write nanolithographic deposition of nucleic acids from scanning probe microscopic tips
JP2008527999A (ja) 長い１本鎖核酸および２本鎖核酸から短い１本鎖核酸を分離するための方法、ならびに関連した生体分子アッセイ法
Jani et al. Soft and hard surface manipulation of nanoporous anodic aluminum oxide (AAO)
Khan et al. General techniques for preparation of nanosensors
中村聡 Orientation Control of Nanorods with the Assisntance of Polymer Brush
Huang et al. Single-Molecule Patterning via DNA Nanostructure Assembly: A Reusable Platform
CN115867677A (zh) 掺入和成像混合物
Vlassiouk et al. Biosensing with nanopores
JPS60194517A (ja) 単分子累積膜成膜方法
Cheng et al. Bio-mediated Assembly of Ordered Nanoparticle Superstructures
Hinds Aligned carbon nanotube membranes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 567318

Kind code of ref document: A

Format of ref document f/p: F

AK Designated states

Kind code of ref document: B1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: B1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

B Later publication of amended claims
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 09979438

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2001912416

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001912416

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001912416

Country of ref document: EP