WO2002008454A3 - Method and device for viable and nonviable prokaryotic and eukaryotic cell quantitation - Google Patents

Method and device for viable and nonviable prokaryotic and eukaryotic cell quantitation Download PDF

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Publication number
WO2002008454A3
WO2002008454A3 PCT/US2001/023358 US0123358W WO0208454A3 WO 2002008454 A3 WO2002008454 A3 WO 2002008454A3 US 0123358 W US0123358 W US 0123358W WO 0208454 A3 WO0208454 A3 WO 0208454A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
cell
quantitating
quantitation
fluorescence
Prior art date
Application number
PCT/US2001/023358
Other languages
French (fr)
Other versions
WO2002008454A2 (en
Inventor
James E Fleming
Jason Buck Somes
Darby Mclean
Jerad R Holcomb
Original Assignee
Genprime Inc
James E Fleming
Jason Buck Somes
Darby Mclean
Jerad R Holcomb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genprime Inc, James E Fleming, Jason Buck Somes, Darby Mclean, Jerad R Holcomb filed Critical Genprime Inc
Priority to AU2001279005A priority Critical patent/AU2001279005A1/en
Publication of WO2002008454A2 publication Critical patent/WO2002008454A2/en
Publication of WO2002008454A3 publication Critical patent/WO2002008454A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]

Abstract

A rapid method for the quantitation of various live cell types is described. The method may include a variety of steps including: 1) suspending the cells in a detergent-like compound; 2) isolating the washed cells by centrifugation or filtration; 3) resuspending the cells in a solution that contains a preservative, a fluorescent dye and a compound such as dequalinium which can be taken up by the cells; 4) measuring the fluorescence increase over time of the cell-dye mixture with a simple fluorometer; and 5) measuring the native fluorescence of the cells. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods; b) quantitating yeast cells in brewing, fermentation and bread making; c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The device, and/or system used for cell quantitation is also disclosed.
PCT/US2001/023358 2000-07-24 2001-07-24 Method and device for viable and nonviable prokaryotic and eukaryotic cell quantitation WO2002008454A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001279005A AU2001279005A1 (en) 2000-07-24 2001-07-24 Method and apparatus for viable and nonviable prokaryotic and eukaryotic cell quantitation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22029800P 2000-07-24 2000-07-24
US60/220,298 2000-07-24

Publications (2)

Publication Number Publication Date
WO2002008454A2 WO2002008454A2 (en) 2002-01-31
WO2002008454A3 true WO2002008454A3 (en) 2002-06-27

Family

ID=22822980

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/023358 WO2002008454A2 (en) 2000-07-24 2001-07-24 Method and device for viable and nonviable prokaryotic and eukaryotic cell quantitation

Country Status (3)

Country Link
US (5) US20020055134A1 (en)
AU (1) AU2001279005A1 (en)
WO (1) WO2002008454A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020055134A1 (en) 2000-07-24 2002-05-09 Fleming James E. Method and apparatus for viable and nonviable prokaryotic and eukaryotic cell quantitation
DE10259302A1 (en) * 2002-12-17 2004-07-08 Henkel Kgaa Method and device for determining germs
TW200506345A (en) * 2003-08-14 2005-02-16 Au Optronics Corp Water quality analysis method using potassium permanganate
US7611863B2 (en) 2004-01-08 2009-11-03 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Selective staining of biomembranes using voltage-sensitive dyes
EP1553396A3 (en) * 2004-01-08 2005-09-21 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Selective staining of biomembranes using voltage-sensitive dyes
DE102006022877B4 (en) 2006-05-15 2009-01-29 Sartorius Stedim Biotech Gmbh Method for determining the viability of cells in cell cultures
US8003314B2 (en) 2007-04-16 2011-08-23 Diagnostic Hybrids, Inc. Methods for direct fluorescent antibody virus detection in liquids
JP2011092104A (en) * 2009-10-30 2011-05-12 Hitachi Engineering & Services Co Ltd Test method and test apparatus for microorganism or the like
US9631219B2 (en) * 2010-02-24 2017-04-25 Nalco Company Metabolic rate indicator for cellular populations
US9664677B2 (en) 2010-04-19 2017-05-30 Massachusetts Institute Of Technology Polymer-nanostructure composition for selective molecular recognition
CN103154264B (en) * 2010-06-07 2015-11-25 耐克思乐生物科学有限责任公司 The mensuration of yeast concn and survival rate
WO2012017194A1 (en) * 2010-07-31 2012-02-09 Advanced Biomedical Limited Method, reagent, and apparatus for detecting a chemical chelator
US9822951B2 (en) 2010-12-06 2017-11-21 Cree, Inc. LED retrofit lens for fluorescent tube
EP3702783A1 (en) * 2013-12-11 2020-09-02 Analiza, Inc. Methods for determining cells such as circulating cancer or fetal cells
EP3304030B1 (en) * 2015-06-01 2021-03-17 Nexcelom Bioscience LLC Methods for cell count and viability measurements
CN109642248B (en) 2016-03-17 2022-09-02 珀金埃尔默健康科学有限责任公司 Detection of cells in a liquid sample
WO2018014002A1 (en) * 2016-07-15 2018-01-18 Pacific Northwest Diabetes Research Institute Systems and methods to facilitate genetic research
CN107164455A (en) * 2017-06-22 2017-09-15 江苏省徐州医药高等职业学校 A kind of viable count method of Thiobacillus thioxidans
US20220381790A1 (en) * 2019-02-11 2022-12-01 University Of Virginia Patent Foundation Selection and blockade of fertilization competent male and female gametes
CN111979112A (en) * 2020-08-25 2020-11-24 郑州金域临床检验中心有限公司 Cell counting device and using method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002632A1 (en) * 1990-07-30 1992-02-20 Sierra Cytometry Fluorescent dyes for identification and enumeration of viable cells in milk

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3586859A (en) * 1970-02-16 1971-06-22 Irwin J Katz Fluorescent cell viability counter
US4783401A (en) * 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5437980A (en) * 1993-05-17 1995-08-01 Molecular Probes, Inc. Phenanthridium dye staining of nucleic acids in living cells
US5563070A (en) * 1993-05-28 1996-10-08 Omron Corporation Method of counting reticulocytes
ATE264307T1 (en) * 1993-06-30 2004-04-15 Abbott Lab INTERCALATORS WITH AFFINITY FOR DNA AND THEIR APPLICATIONS
US6277655B1 (en) * 1994-10-13 2001-08-21 Solvo Biotechnology Assay and reagent kit for evaluation of multi-drug resistance in cells
AU7603096A (en) * 1995-11-08 1997-05-29 Pioneer Hi-Bred International, Inc. Methods of assessing viability of microbial cultures
WO1997024616A1 (en) * 1995-12-29 1997-07-10 Ishihara Sangyo Kaisha, Ltd. Method for determining viable cell count
US6673568B1 (en) * 1999-10-25 2004-01-06 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
US20020055134A1 (en) * 2000-07-24 2002-05-09 Fleming James E. Method and apparatus for viable and nonviable prokaryotic and eukaryotic cell quantitation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002632A1 (en) * 1990-07-30 1992-02-20 Sierra Cytometry Fluorescent dyes for identification and enumeration of viable cells in milk

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KOVARIK ET AL.: "Estimation of viable cell count after fluorescein diacetate staining using phosphoimager analysis", BIOTECHNIQUES, vol. 27, no. 4, - 1999, pages 685 - 688, XP001026301 *
STEWARD N ET AL: "A new methodology for plant cell viability assessment using intracellular esterase activity.", PLANT CELL REPORTS, vol. 19, no. 2, December 1999 (1999-12-01), pages 171 - 176, XP001027085, ISSN: 0721-7714 *

Also Published As

Publication number Publication date
US8748159B2 (en) 2014-06-10
US7906295B2 (en) 2011-03-15
US20020055134A1 (en) 2002-05-09
US20050282244A1 (en) 2005-12-22
WO2002008454A2 (en) 2002-01-31
US20120164677A1 (en) 2012-06-28
US20110136159A1 (en) 2011-06-09
US8071326B2 (en) 2011-12-06
AU2001279005A1 (en) 2002-02-05
US20090104652A1 (en) 2009-04-23
US7527924B2 (en) 2009-05-05

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