WO2002009868A3 - Apparatus for generating a temperature gradient and methods for using the gradient to characterize molecular interactions - Google Patents

Apparatus for generating a temperature gradient and methods for using the gradient to characterize molecular interactions Download PDF

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Publication number
WO2002009868A3
WO2002009868A3 PCT/US2001/023831 US0123831W WO0209868A3 WO 2002009868 A3 WO2002009868 A3 WO 2002009868A3 US 0123831 W US0123831 W US 0123831W WO 0209868 A3 WO0209868 A3 WO 0209868A3
Authority
WO
WIPO (PCT)
Prior art keywords
gradient
methods
generating
molecular interactions
temperature gradients
Prior art date
Application number
PCT/US2001/023831
Other languages
French (fr)
Other versions
WO2002009868A2 (en
Inventor
Martin Blumenfeld
Mark Fisher
Fred Williamson
Gregory T Cibuzar
Ness Brian G Van
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Priority to EP01961792A priority Critical patent/EP1307293A2/en
Priority to AU2001283031A priority patent/AU2001283031A1/en
Priority to CA002417889A priority patent/CA2417889A1/en
Priority to JP2002515410A priority patent/JP2004505255A/en
Publication of WO2002009868A2 publication Critical patent/WO2002009868A2/en
Publication of WO2002009868A3 publication Critical patent/WO2002009868A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/54—Heating or cooling apparatus; Heat insulating devices using spatial temperature gradients
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

Abstract

A novel apparatus (100) for generating temperature gradients is described. The apparatus includes a semiconductive wafer (110) and electrical connectors (114a, 114b) attached to, preferably, one of the edges of the wafer. Methods for transferring the temperature gradients to strata are described. The temperature gradients on the strata can be used for analyses of molecules, particularly biological macromolecules. The present invention also includes improved methods for determining the thermal stability of binding complexes.
PCT/US2001/023831 2000-08-01 2001-07-30 Apparatus for generating a temperature gradient and methods for using the gradient to characterize molecular interactions WO2002009868A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP01961792A EP1307293A2 (en) 2000-08-01 2001-07-30 Apparatus for generating a temperature gradient and methods for using the gradient to characterize molecular interactions
AU2001283031A AU2001283031A1 (en) 2000-08-01 2001-07-30 Apparatus for generating a temperature gradient and methods for using the gradient to characterize molecular interactions
CA002417889A CA2417889A1 (en) 2000-08-01 2001-07-30 Apparatus for generating a temperature gradient and methods for using the gradient to characterize molecular interactions
JP2002515410A JP2004505255A (en) 2000-08-01 2001-07-30 Apparatus for forming a temperature gradient and methods of using the gradient to characterize molecular interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/630,172 US6544477B1 (en) 2000-08-01 2000-08-01 Apparatus for generating a temperature gradient
US09/630,172 2000-08-01

Publications (2)

Publication Number Publication Date
WO2002009868A2 WO2002009868A2 (en) 2002-02-07
WO2002009868A3 true WO2002009868A3 (en) 2002-06-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/023831 WO2002009868A2 (en) 2000-08-01 2001-07-30 Apparatus for generating a temperature gradient and methods for using the gradient to characterize molecular interactions

Country Status (6)

Country Link
US (3) US6544477B1 (en)
EP (1) EP1307293A2 (en)
JP (1) JP2004505255A (en)
AU (1) AU2001283031A1 (en)
CA (1) CA2417889A1 (en)
WO (1) WO2002009868A2 (en)

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US7745124B2 (en) 2004-04-28 2010-06-29 Eisai R&D Management Co., Ltd. Hybridization method

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WO2002099386A2 (en) * 2001-06-07 2002-12-12 Proligo Llc Microcalorimetric detection of analytes and binding events
US20060134683A1 (en) * 2001-10-23 2006-06-22 Michael Sogard Methods and devices for hybridization and binding assays using thermophoresis
US7244611B2 (en) * 2001-10-23 2007-07-17 Nikon Research Corporation Of America Methods and devices for hybridization and binding assays using thermophoresis
AU2002359329A1 (en) * 2001-10-30 2003-05-12 The Texas A And M University System Method and apparatus for temperature gradient microfluidics
US7232547B2 (en) * 2002-03-22 2007-06-19 Marshfield Clinic Apparatus and method for testing and continuously reading low-volume samples
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
WO2005058501A1 (en) * 2002-09-09 2005-06-30 Bjs Company Ltd Heating samples in specimen carriers
US8398632B1 (en) * 2003-06-10 2013-03-19 Medtronic Cryocath Lp Surgical clamp having treatment elements
US7044946B2 (en) 2003-06-10 2006-05-16 Cryocath Technologies Inc. Surgical clamp having treatment elements
US7819860B2 (en) 2003-06-10 2010-10-26 Medtronic Cryocath Lp Surgical clamp having trasmurality assessment capabilities
US20050237528A1 (en) * 2003-09-19 2005-10-27 Oldham Mark F Transparent heater for thermocycling
US7570443B2 (en) * 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
WO2005050571A2 (en) * 2003-11-21 2005-06-02 Koninklijke Philips Electronics, N.V. Ultrasound imaging system and method having adaptive selection of image frame rate and/or number of echo samples averaged
JP4179169B2 (en) * 2004-01-08 2008-11-12 カシオ計算機株式会社 Analysis equipment
JP4667769B2 (en) * 2004-06-11 2011-04-13 信越ポリマー株式会社 Substrate storage container
US7585663B2 (en) * 2004-08-26 2009-09-08 Applied Biosystems, Llc Thermal device, system, and method, for fluid processing device
WO2007062666A1 (en) * 2005-12-02 2007-06-07 Technical University Of Denmark Fluidics device for assay
US20090099045A1 (en) * 2007-10-10 2009-04-16 Biotex, Inc. Methods and devices for molecular association and imaging
WO2012033914A1 (en) * 2010-09-09 2012-03-15 Battelle Memorial Institute Heating a short section of tape or wire to a controlled temperature
US11513076B2 (en) 2016-06-15 2022-11-29 Ludwig-Maximilians-Universität München Single molecule detection or quantification using DNA nanotechnology

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Also Published As

Publication number Publication date
EP1307293A2 (en) 2003-05-07
US6544477B1 (en) 2003-04-08
CA2417889A1 (en) 2002-02-07
WO2002009868A2 (en) 2002-02-07
US6733729B2 (en) 2004-05-11
AU2001283031A1 (en) 2002-02-13
US6610470B2 (en) 2003-08-26
US20020015995A1 (en) 2002-02-07
US20020015996A1 (en) 2002-02-07
JP2004505255A (en) 2004-02-19

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