WO2002010432A3 - Triphosphate oligonucleotide modification reagents and uses thereof - Google Patents

Triphosphate oligonucleotide modification reagents and uses thereof Download PDF

Info

Publication number
WO2002010432A3
WO2002010432A3 PCT/US2001/023775 US0123775W WO0210432A3 WO 2002010432 A3 WO2002010432 A3 WO 2002010432A3 US 0123775 W US0123775 W US 0123775W WO 0210432 A3 WO0210432 A3 WO 0210432A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotides
methods
modification reagents
oligonucleotide modification
preparation
Prior art date
Application number
PCT/US2001/023775
Other languages
French (fr)
Other versions
WO2002010432A2 (en
Inventor
David A Schwartz
Richard I Hogrefe
Original Assignee
David A Schwartz
Richard I Hogrefe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by David A Schwartz, Richard I Hogrefe filed Critical David A Schwartz
Priority to EP01956020A priority Critical patent/EP1305327A2/en
Priority to AU2001278061A priority patent/AU2001278061A1/en
Publication of WO2002010432A2 publication Critical patent/WO2002010432A2/en
Publication of WO2002010432A3 publication Critical patent/WO2002010432A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Hydrazino, oxyamino and carbonyl-based monomers and methods for incorporation into oligonucleotides during enzymatic synthesis are provided. Modified oligonucleotides are provided that incorporate the monomers provided herein. Immobilized oligonucleotides and oligonucleotide conjugates that contain covalent hydrazone or oxime linkages are provided. Methods for preparation of surface bound oligonucleotides are provided. Methods for the preparation of oligonucleotide conjugates are also provided.
PCT/US2001/023775 2000-08-01 2001-07-27 Triphosphate oligonucleotide modification reagents and uses thereof WO2002010432A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01956020A EP1305327A2 (en) 2000-08-01 2001-07-27 Triphosphate oligonucleotide modification reagents and uses thereof
AU2001278061A AU2001278061A1 (en) 2000-08-01 2001-07-27 Triphosphate oligonucleotide modification reagents and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/630,627 US6686461B1 (en) 2000-03-22 2000-08-01 Triphosphate oligonucleotide modification reagents and uses thereof
US09/630,627 2000-08-01

Publications (2)

Publication Number Publication Date
WO2002010432A2 WO2002010432A2 (en) 2002-02-07
WO2002010432A3 true WO2002010432A3 (en) 2002-04-18

Family

ID=24527934

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/023775 WO2002010432A2 (en) 2000-08-01 2001-07-27 Triphosphate oligonucleotide modification reagents and uses thereof

Country Status (4)

Country Link
US (3) US6686461B1 (en)
EP (1) EP1305327A2 (en)
AU (1) AU2001278061A1 (en)
WO (1) WO2002010432A2 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050266427A1 (en) * 2002-01-15 2005-12-01 Schwartz David A Biomolecule/polymer conjugates
EP1556325A4 (en) * 2002-09-20 2007-09-19 Integrated Dna Tech Inc Anthraquinone quencher dyes, their methods of preparation and use
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
FR2873697B1 (en) * 2004-07-28 2006-10-27 Commissariat Energie Atomique PHOTOLABILE PROTECTIVE GROUPING COUPLING AGENTS AND THEIR USES, IN PARTICULAR FOR THE FUNCTIONALIZATION OF SOLID SUPPORTS
US8212020B2 (en) * 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
EP1907560B1 (en) * 2005-05-20 2013-01-23 Integrated DNA Technologies, Inc. Compounds and methods for labeling oligonucleotides
US7405281B2 (en) * 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US8541555B2 (en) * 2006-04-18 2013-09-24 Solulink Biosciences, Inc. Hydrazone-based and oxime-based fluorescent and chromophoric/pro-fluorescent and pro-chromophoric reagents and linkers
LT2056845T (en) 2006-08-08 2017-11-27 Rheinische Friedrich-Wilhelms-Universität Bonn Structure and use of 5` phosphate oligonucleotides
WO2011100493A1 (en) * 2010-02-12 2011-08-18 Solulink, Inc. Preparation and/or purification of oligonucleotide conjugates
US9222936B2 (en) 2007-04-18 2015-12-29 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
EP2297323A1 (en) 2008-05-21 2011-03-23 Hartmann, Gunther 5' triphosphate oligonucleotide with blunt end and uses thereof
US20100062518A1 (en) * 2008-09-09 2010-03-11 Sukanta Banerjee Concentrating White Blood Cells for DNA Extraction from a Leukodepleted Blood Sample
US8309306B2 (en) * 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
US8034923B1 (en) * 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
US20110223588A1 (en) * 2010-03-09 2011-09-15 Biosample Llc Solid Phase Nucleic Acid Extraction From Small Sample Volumes, and Release of Controlled Quantities
JP5886828B2 (en) 2010-03-26 2016-03-16 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド Method for enhancing hybridization of nucleic acids
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
EP2896696B1 (en) 2010-09-07 2017-12-27 Integrated Dna Technologies, Inc. Modifications for antisense compounds
EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
JP7297408B2 (en) 2015-02-06 2023-06-26 セル アイディーエックス， インコーポレイテッド Immunoreagent coupled with antigen
DK3555313T3 (en) * 2016-12-16 2021-08-09 Aratome Llc MOLECULAR DETECTION USING LIGERATION AMPLIFICATION
US20210032285A1 (en) * 2019-03-11 2021-02-04 Cell Idx, Inc. Methods, compositions, and kits for trapping modified biomolecules

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4707440A (en) * 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US5047519A (en) * 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5151507A (en) * 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5876938A (en) * 1996-08-05 1999-03-02 Prolinx, Incorporated Use of boron-containing polynucleotides as diagnostic agents

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4006117A (en) 1973-01-24 1977-02-01 Hooker Chemicals & Plastics Corporation Amine phosphite antioxidants
CS175047B1 (en) 1974-04-25 1977-04-29
US3939123A (en) 1974-06-18 1976-02-17 Union Carbide Corporation Lightly cross-linked polyurethane hydrogels based on poly(alkylene ether) polyols
NZ180198A (en) 1976-03-04 1978-11-13 New Zealand Dev Finance Cationic ion exchanger with a cross-linked carbohydrate marix
DE2621974A1 (en) 1976-05-18 1977-11-24 Max Planck Gesellschaft PROCESS FOR PRODUCING A COVALENT BOND WITH BIOLOGICALLY ACTIVE MATERIALS
US4162355A (en) 1976-06-30 1979-07-24 Board Of Regents, For And On Behalf Of The University Of Florida Copolymers of (a) aminimides and (b) vinyl pendant primary halomethy monomers useful for affinity chromatography
US4175183A (en) 1977-03-01 1979-11-20 Development Finance Corporation Of New Zealand Hydroxyalkylated cross-linked regenerated cellulose and method of preparation thereof
US4180524A (en) 1978-02-16 1979-12-25 Phillips Petroleum Company Disproportionation/double-bond isomerization of olefins
JPS55116259A (en) 1979-03-01 1980-09-06 Fuji Photo Film Co Ltd Microimmunoassay method
US4241537A (en) 1979-05-10 1980-12-30 W. R. Grace & Co. Plant growth media utilizing polyurethane hydrogel
DE3027198A1 (en) 1980-07-18 1982-02-11 Bayer Ag, 5090 Leverkusen SOLID, PRE-DISPERSABLE WATER-DISPERSIBLE, ISOCYANATE GROUPS, A METHOD FOR THE PRODUCTION OF AQUEOUS PLASTIC DISPERSIONS USING THESE PRE-PLASTICS, AND THEIR IMPROVERS
JPS5745460A (en) 1980-09-02 1982-03-15 Fuji Photo Film Co Ltd Inspection sheet for measuring trace component and inspecting method using said sheet
DE3170135D1 (en) 1980-09-02 1985-05-30 Fuji Photo Film Co Ltd Method for immunochemical measurement of trace components
US4439585A (en) 1980-11-12 1984-03-27 Tyndale Plains-Hunter, Ltd. Polyurethane diacrylate compositions as carrier for pharmacological agents
US4507230A (en) 1982-05-12 1985-03-26 Research Corporation Peptide synthesis reagents and method of use
US4485227A (en) 1983-06-16 1984-11-27 Howmedica, Inc. Biocompatible poly-(ether-urethane-urea) and process for its preparation
AU3844485A (en) 1984-02-09 1985-08-15 Enzo Biochem Inc. Heterologous detection of cabeled dna
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4833251A (en) 1985-06-25 1989-05-23 Siska Diagnostics, Inc. Compounds for tagging nucleic acid probes
US5210203A (en) 1985-06-25 1993-05-11 Siska Diagnostics, Inc. Sulfonamide-derivative compounds for tagging nucleic acid probes
US5130446A (en) 1985-06-25 1992-07-14 Siska Diagnostics, Inc. Fluorescent compounds for tagging nucleic acid probes
US5627027A (en) 1986-04-18 1997-05-06 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5268486A (en) 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5569587A (en) 1986-04-18 1996-10-29 Carnegie Mellon University Method for labeling and detecting materials employing luminescent arysulfonate cyanine dyes
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US4874813A (en) 1987-02-09 1989-10-17 Shannessy Daniel J O Proteins bound to a marker or solid phase support matrix using a hydrazone linkage
US4927879A (en) 1988-02-25 1990-05-22 Purdue Research Foundation Method for solid phase membrane mimetics
US4931498A (en) 1988-02-25 1990-06-05 Purdue Research Foundation Immobilized artificial membranes
CA1333366C (en) 1988-09-26 1994-12-06 Soumitra Shankar Ghosh Enzyme-nucleic acid hydrazone and hydrazide conjugate compositions and methods
US5066789A (en) 1988-09-30 1991-11-19 Neorx Corporation Targeting substance-diagnostic/therapeutic agent conjugates having Schiff base linkages
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
IL93432A (en) 1989-02-24 1994-02-27 Johnson Mathey Inc Hydrazines and hydrazides, their conjugates with macromolecules and such conjugates labelled with metal ions
US5206370A (en) 1989-02-24 1993-04-27 Johnson Matthey, Inc. Certain pyridyl hydrazines and hydrazides useful for protein labeling
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5092992A (en) 1989-06-07 1992-03-03 J. T. Baker Inc. Polyethyleneimine matrixes for affinity chromatography
US6001826A (en) 1989-12-21 1999-12-14 Anormed, Inc. Chemical compounds
DE4000038A1 (en) 1990-01-03 1991-07-04 Hoechst Ag Biaxially oriented polyester esp. PET film substrate - with specified surface tension, modulus and refractive index for good adhesion
US5391785A (en) 1990-01-16 1995-02-21 La Jolla Pharmaceutial Company Intermediates for providing functional groups on the 5' end of oligonucleotides
US5328603A (en) 1990-03-20 1994-07-12 The Center For Innovative Technology Lignocellulosic and cellulosic beads for use in affinity and immunoaffinity chromatography of high molecular weight proteins
US5723286A (en) 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
US5474895A (en) 1990-11-14 1995-12-12 Siska Diagnostics Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
CA2099574A1 (en) 1991-01-04 1992-07-05 Noubar B. Afeyan Sulfonamide bonded hydrophilic coatings
US5403750A (en) 1991-03-06 1995-04-04 W. R. Grace & Co.-Conn. Biocompatible, low protein adsorption affinity matrix
US5334640A (en) 1992-04-08 1994-08-02 Clover Consolidated, Ltd. Ionically covalently crosslinked and crosslinkable biocompatible encapsulation compositions and methods
GB9223168D0 (en) 1992-11-05 1992-12-16 Johnson Matthey Plc Improvements in molecule labelling
US5547835A (en) 1993-01-07 1996-08-20 Sequenom, Inc. DNA sequencing by mass spectrometry
US6074823A (en) 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US6087186A (en) 1993-07-16 2000-07-11 Irori Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
AU695552B2 (en) 1993-11-16 1998-08-13 Genta Incorporated Chirally enriched synthetic phosphonate oligomers
US5955597A (en) 1993-11-16 1999-09-21 Genta, Incorporated Chirally enriched synthetic phosphate oligomers
EP0729474A4 (en) 1993-11-16 1998-10-21 Genta Inc Synthetic oligomers having chirally pure phosphonate internucleosidyl linkages mixed with non-phosphonate internucleosidyl linkages
US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
US5824473A (en) 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5952172A (en) 1993-12-10 1999-09-14 California Institute Of Technology Nucleic acid mediated electron transfer
US5854410A (en) 1994-03-31 1998-12-29 Genta Incorporated Oligonucleoside cleavage compounds and therapies
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5789576A (en) 1994-12-09 1998-08-04 Genta Incorporated Methylphosphonate dimer synthesis
CN1661115A (en) 1995-03-10 2005-08-31 梅索磅秤技术有限公司 Multi-array, multi-specific electrochemiluminescence testing
US6017496A (en) 1995-06-07 2000-01-25 Irori Matrices with memories and uses thereof
US5925562A (en) 1995-04-25 1999-07-20 Irori Remotely programmable matrices with memories
US5741462A (en) 1995-04-25 1998-04-21 Irori Remotely programmable matrices with memories
US5961923A (en) 1995-04-25 1999-10-05 Irori Matrices with memories and uses thereof
US6025129A (en) 1995-04-25 2000-02-15 Irori Remotely programmable matrices with memories and uses thereof
US5751629A (en) 1995-04-25 1998-05-12 Irori Remotely programmable matrices with memories
US5874214A (en) 1995-04-25 1999-02-23 Irori Remotely programmable matrices with memories
AU705549B2 (en) 1995-06-07 1999-05-27 Promega Biosciences, Inc. Phosphonic acid-based cationic lipids
WO1996040662A2 (en) 1995-06-07 1996-12-19 Cellpro, Incorporated Aminooxy-containing linker compounds and their application in conjugates
US5856571A (en) 1995-06-07 1999-01-05 Cellpro, Incorporated Semicarbazide-containing linker compounds for formation of stably-linked conjugates and methods related thereto
NZ313839A (en) 1995-07-21 1998-12-23 Genta Inc Amide-based cationic lipids
US6124444A (en) 1995-11-03 2000-09-26 Nec Research Institute, Inc. DNA sequences useful for computation
US6136274A (en) 1996-10-07 2000-10-24 Irori Matrices with memories in automated drug discovery and units therefor
WO1998015825A2 (en) 1996-10-07 1998-04-16 Irori Matrices with memories in automated drug discovery and units therefor
US6127533A (en) 1997-02-14 2000-10-03 Isis Pharmaceuticals, Inc. 2'-O-aminooxy-modified oligonucleotides
US6172209B1 (en) 1997-02-14 2001-01-09 Isis Pharmaceuticals Inc. Aminooxy-modified oligonucleotides and methods for making same
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US6034135A (en) 1997-03-06 2000-03-07 Promega Biosciences, Inc. Dimeric cationic lipids
US5877220A (en) 1997-03-06 1999-03-02 Genta, Incorporated Amide-based oligomeric cationic lipids
US5972639A (en) 1997-07-24 1999-10-26 Irori Fluorescence-based assays for measuring cell proliferation
US6110630A (en) 1998-06-18 2000-08-29 Beckman Coulter, Inc. Efficient activated cyanine dyes
US6576478B1 (en) 1998-07-14 2003-06-10 Zyomyx, Inc. Microdevices for high-throughput screening of biomolecules
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
ATE277065T1 (en) 1999-01-05 2004-10-15 Bio Merieux FUNCTIONALIZED COMPOUND, OPTIONALLY LABELED POLYNUCLEOTIDES AND METHOD FOR DETECTING A TARGET NUCLEIC ACID
US6339147B1 (en) 1999-07-29 2002-01-15 Epoch Biosciences, Inc. Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture and detection of nucleic acids
US6319674B1 (en) * 1999-09-16 2001-11-20 Agilent Technologies, Inc. Methods for attaching substances to surfaces

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4707440A (en) * 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US5047519A (en) * 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5151507A (en) * 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5876938A (en) * 1996-08-05 1999-03-02 Prolinx, Incorporated Use of boron-containing polynucleotides as diagnostic agents

Also Published As

Publication number Publication date
US7999098B2 (en) 2011-08-16
AU2001278061A1 (en) 2002-02-13
US6686461B1 (en) 2004-02-03
US20040214199A1 (en) 2004-10-28
WO2002010432A2 (en) 2002-02-07
EP1305327A2 (en) 2003-05-02
US7173125B2 (en) 2007-02-06
US20070179288A1 (en) 2007-08-02

Similar Documents

Publication Publication Date Title
WO2002010432A3 (en) Triphosphate oligonucleotide modification reagents and uses thereof
WO2002010431A3 (en) Functional biopolymer modification reagents and uses thereof
AU2002227156A1 (en) Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AU3719700A (en) Enzymatic dna molecules that contain modified nucleotides
AU2002335613A1 (en) Biochips comprising nucleic acid/protein conjugates
AU4903101A (en) Isolated nucleic acid molecules encoding cancer associated antigens, the antigens per se, and uses thereof
AU3072101A (en) Pcr amplification on microarrays of gel immobilized oligonucleotides
WO2001055370A3 (en) MODULATION OF OLIGONUCLEOTIDE CpG-MEDIATED IMMUNE STIMULATION BY POSITIONAL MODIFICATION OF NUCLEOSIDES
WO2001094630A3 (en) Compositions and methods for array-based nucleic acid hybridization
EP2567964A3 (en) Monomer Compositions for the Synthesis of RNA, Methods of Synthesis, and Methods of Deprotection
EP2314693A3 (en) Modulation of oligonucleotide CpG-mediated immune stimulation by positional modification of nucleosides
AU2001279134A1 (en) Nucleotide incorporating enzymes
AU4332399A (en) Rna polymerase chain reaction
AU4518601A (en) Microarray hybridization chamber
HK1112007A1 (en) Peptide acceptor ligation methods
WO2004029277A3 (en) Oligonucleotide conjugates
AU2002313699A1 (en) Enzymatic nucleic acid peptide conjugates
AU2003302165A1 (en) Prostate specific antigens, conjugates thereof, methods for their preparation and uses thereof.
EP1224202A4 (en) Antisense modulation of integrin-linked kinase expression
WO2003035667A3 (en) Telomerase interference
EP1248633A4 (en) Antisense modulation of glycogen synthase kinase 3 beta expression
WO2002014482A3 (en) Polyketides and their synthesis
WO2002057418A3 (en) Methods of producing polyketide synthase mutants and compositions and uses thereof
WO2003044168A3 (en) Facilitation of rna interference
WO2002101051A3 (en) Genes and proteins for the biosynthesis of anthramycin

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2001956020

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001956020

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP