WO2002018660A2 - Detection of varicella-zoster virus - Google Patents
Detection of varicella-zoster virus Download PDFInfo
- Publication number
- WO2002018660A2 WO2002018660A2 PCT/US2001/041980 US0141980W WO0218660A2 WO 2002018660 A2 WO2002018660 A2 WO 2002018660A2 US 0141980 W US0141980 W US 0141980W WO 0218660 A2 WO0218660 A2 WO 0218660A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gene
- pair
- probes
- vzv
- probe
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract description 29
- 241000701085 Human alphaherpesvirus 3 Species 0.000 title description 135
- 239000000523 sample Substances 0.000 claims abstract description 172
- 238000000034 method Methods 0.000 claims abstract description 81
- 239000012472 biological sample Substances 0.000 claims abstract description 41
- 238000004519 manufacturing process Methods 0.000 claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 claims description 271
- 150000007523 nucleic acids Chemical class 0.000 claims description 74
- 108020004707 nucleic acids Proteins 0.000 claims description 70
- 102000039446 nucleic acids Human genes 0.000 claims description 70
- 230000003321 amplification Effects 0.000 claims description 59
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 59
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims description 47
- 230000001351 cycling effect Effects 0.000 claims description 32
- 238000009396 hybridization Methods 0.000 claims description 20
- 208000015181 infectious disease Diseases 0.000 claims description 20
- 239000002773 nucleotide Substances 0.000 claims description 20
- 125000003729 nucleotide group Chemical group 0.000 claims description 20
- 238000002844 melting Methods 0.000 claims description 14
- 230000008018 melting Effects 0.000 claims description 14
- 210000001519 tissue Anatomy 0.000 claims description 9
- 230000002500 effect on skin Effects 0.000 claims description 8
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical group O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 8
- 239000013068 control sample Substances 0.000 claims description 6
- SNBCLPGEMZEWLU-QXFUBDJGSA-N 2-chloro-n-[[(2r,3s,5r)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methyl]acetamide Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CNC(=O)CCl)[C@@H](O)C1 SNBCLPGEMZEWLU-QXFUBDJGSA-N 0.000 claims description 5
- 230000000574 ganglionic effect Effects 0.000 claims description 5
- 206010036790 Productive cough Diseases 0.000 claims description 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 210000005013 brain tissue Anatomy 0.000 claims description 4
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 210000004072 lung Anatomy 0.000 claims description 4
- 210000003802 sputum Anatomy 0.000 claims description 4
- 208000024794 sputum Diseases 0.000 claims description 4
- 210000002700 urine Anatomy 0.000 claims description 4
- LIZDKDDCWIEQIN-UHFFFAOYSA-N 6-[2-[5-(3-ethyl-1,1-dimethyl-6,8-disulfobenzo[e]indol-2-ylidene)penta-1,3-dienyl]-1,1-dimethyl-6,8-disulfobenzo[e]indol-3-ium-3-yl]hexanoate Chemical compound C1=CC2=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C=C2C(C2(C)C)=C1N(CC)\C2=C\C=C\C=C\C1=[N+](CCCCCC([O-])=O)C2=CC=C(C(=CC(=C3)S(O)(=O)=O)S(O)(=O)=O)C3=C2C1(C)C LIZDKDDCWIEQIN-UHFFFAOYSA-N 0.000 claims description 3
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims description 3
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims description 3
- 239000000356 contaminant Substances 0.000 claims description 2
- 229940035893 uracil Drugs 0.000 claims description 2
- 238000003753 real-time PCR Methods 0.000 abstract description 8
- 239000013615 primer Substances 0.000 description 84
- 239000002987 primer (paints) Substances 0.000 description 30
- 108020004414 DNA Proteins 0.000 description 22
- 230000000875 corresponding effect Effects 0.000 description 20
- 238000003556 assay Methods 0.000 description 18
- 238000006243 chemical reaction Methods 0.000 description 13
- 210000004027 cell Anatomy 0.000 description 12
- 238000000137 annealing Methods 0.000 description 10
- 208000007514 Herpes zoster Diseases 0.000 description 9
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- 108091034117 Oligonucleotide Proteins 0.000 description 8
- 241000700605 Viruses Species 0.000 description 7
- 238000004113 cell culture Methods 0.000 description 7
- 238000012546 transfer Methods 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 230000005284 excitation Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 230000003612 virological effect Effects 0.000 description 6
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 5
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000011109 contamination Methods 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- 239000002751 oligonucleotide probe Substances 0.000 description 5
- 108091026890 Coding region Proteins 0.000 description 4
- 239000003155 DNA primer Substances 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 108700026244 Open Reading Frames Proteins 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 241000700584 Simplexvirus Species 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 229960005486 vaccine Drugs 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 201000006082 Chickenpox Diseases 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- -1 DNA or RNA Chemical class 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 206010046980 Varicella Diseases 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000000295 emission spectrum Methods 0.000 description 3
- 238000000695 excitation spectrum Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000011880 melting curve analysis Methods 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- YJCCSLGGODRWKK-NSCUHMNNSA-N 4-Acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid Chemical compound OS(=O)(=O)C1=CC(NC(=O)C)=CC=C1\C=C\C1=CC=C(N=C=S)C=C1S(O)(=O)=O YJCCSLGGODRWKK-NSCUHMNNSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000701022 Cytomegalovirus Species 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 241000230501 Equine herpesvirus sp. Species 0.000 description 2
- 241000700588 Human alphaherpesvirus 1 Species 0.000 description 2
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 2
- 238000002944 PCR assay Methods 0.000 description 2
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 2
- 241000701093 Suid alphaherpesvirus 1 Species 0.000 description 2
- 241000589499 Thermus thermophilus Species 0.000 description 2
- 206010058874 Viraemia Diseases 0.000 description 2
- 239000003570 air Substances 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 229910052786 argon Inorganic materials 0.000 description 2
- 230000002238 attenuated effect Effects 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000003771 laboratory diagnosis Methods 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000002515 oligonucleotide synthesis Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000006862 quantum yield reaction Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- 241001529453 unidentified herpesvirus Species 0.000 description 2
- 229940021648 varicella vaccine Drugs 0.000 description 2
- 238000001429 visible spectrum Methods 0.000 description 2
- OBYNJKLOYWCXEP-UHFFFAOYSA-N 2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-4-isothiocyanatobenzoate Chemical compound C=12C=CC(=[N+](C)C)C=C2OC2=CC(N(C)C)=CC=C2C=1C1=CC(N=C=S)=CC=C1C([O-])=O OBYNJKLOYWCXEP-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- QXYRRCOJHNZVDJ-UHFFFAOYSA-N 4-pyren-1-ylbutanoic acid Chemical compound C1=C2C(CCCC(=O)O)=CC=C(C=C3)C2=C2C3=CC=CC2=C1 QXYRRCOJHNZVDJ-UHFFFAOYSA-N 0.000 description 1
- YERWMQJEYUIJBO-UHFFFAOYSA-N 5-chlorosulfonyl-2-[3-(diethylamino)-6-diethylazaniumylidenexanthen-9-yl]benzenesulfonate Chemical compound C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=C(S(Cl)(=O)=O)C=C1S([O-])(=O)=O YERWMQJEYUIJBO-UHFFFAOYSA-N 0.000 description 1
- TXSWURLNYUQATR-UHFFFAOYSA-N 6-amino-2-(3-ethenylsulfonylphenyl)-1,3-dioxobenzo[de]isoquinoline-5,8-disulfonic acid Chemical compound O=C1C(C2=3)=CC(S(O)(=O)=O)=CC=3C(N)=C(S(O)(=O)=O)C=C2C(=O)N1C1=CC=CC(S(=O)(=O)C=C)=C1 TXSWURLNYUQATR-UHFFFAOYSA-N 0.000 description 1
- YALJZNKPECPZAS-UHFFFAOYSA-N 7-(diethylamino)-3-(4-isothiocyanatophenyl)-4-methylchromen-2-one Chemical compound O=C1OC2=CC(N(CC)CC)=CC=C2C(C)=C1C1=CC=C(N=C=S)C=C1 YALJZNKPECPZAS-UHFFFAOYSA-N 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000264368 Coptotermes lacteus Species 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 101150026402 DBP gene Proteins 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 1
- 208000009889 Herpes Simplex Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000203367 Methanothermus fervidus Species 0.000 description 1
- QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 description 1
- 108091061960 Naked DNA Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 241000283283 Orcinus orca Species 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 102000009572 RNA Polymerase II Human genes 0.000 description 1
- 108010009460 RNA Polymerase II Proteins 0.000 description 1
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 108091027981 Response element Proteins 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 229910052771 Terbium Inorganic materials 0.000 description 1
- 241000042515 Tetraselmis rubens Species 0.000 description 1
- 241000191098 Thermoflexibacter ruber Species 0.000 description 1
- 240000001085 Trapa natans Species 0.000 description 1
- 101150008036 UL29 gene Proteins 0.000 description 1
- 101150068034 UL30 gene Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- UMGDCJDMYOKAJW-UHFFFAOYSA-N aminothiocarboxamide Natural products NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000000337 buffer salt Substances 0.000 description 1
- UIZLQMLDSWKZGC-UHFFFAOYSA-N cadmium helium Chemical compound [He].[Cd] UIZLQMLDSWKZGC-UHFFFAOYSA-N 0.000 description 1
- 235000010410 calcium alginate Nutrition 0.000 description 1
- 239000000648 calcium alginate Substances 0.000 description 1
- 229960002681 calcium alginate Drugs 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000003271 compound fluorescence assay Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 239000005549 deoxyribonucleoside Substances 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- KPBGWWXVWRSIAY-UHFFFAOYSA-L disodium;2',4',5',7'-tetraiodo-6-isothiocyanato-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate Chemical compound [Na+].[Na+].O1C(=O)C2=CC=C(N=C=S)C=C2C21C1=CC(I)=C([O-])C(I)=C1OC1=C(I)C([O-])=C(I)C=C21 KPBGWWXVWRSIAY-UHFFFAOYSA-L 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 210000000609 ganglia Anatomy 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 229940099472 immunoglobulin a Drugs 0.000 description 1
- 229940027941 immunoglobulin g Drugs 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 229910021644 lanthanide ion Inorganic materials 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- DLBFLQKQABVKGT-UHFFFAOYSA-L lucifer yellow dye Chemical compound [Li+].[Li+].[O-]S(=O)(=O)C1=CC(C(N(C(=O)NN)C2=O)=O)=C3C2=CC(S([O-])(=O)=O)=CC3=C1N DLBFLQKQABVKGT-UHFFFAOYSA-L 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 208000013435 necrotic lesion Diseases 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000004789 organ system Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 210000001428 peripheral nervous system Anatomy 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 230000010322 reactivation of latent virus Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 238000011309 routine diagnosis Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 231100000046 skin rash Toxicity 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 210000003594 spinal ganglia Anatomy 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- GZCRRIHWUXGPOV-UHFFFAOYSA-N terbium atom Chemical compound [Tb] GZCRRIHWUXGPOV-UHFFFAOYSA-N 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2001296864A AU2001296864A1 (en) | 2000-08-31 | 2001-08-31 | Detection of varicella-zoster virus |
EP01977773A EP1360324A2 (en) | 2000-08-31 | 2001-08-31 | Detection of varicella-zoster virus |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22949900P | 2000-08-31 | 2000-08-31 | |
US60/229,499 | 2000-08-31 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2002018660A2 true WO2002018660A2 (en) | 2002-03-07 |
WO2002018660A9 WO2002018660A9 (en) | 2003-05-08 |
WO2002018660A3 WO2002018660A3 (en) | 2003-08-14 |
Family
ID=22861502
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/041980 WO2002018660A2 (en) | 2000-08-31 | 2001-08-31 | Detection of varicella-zoster virus |
Country Status (4)
Country | Link |
---|---|
US (2) | US6849407B2 (en) |
EP (1) | EP1360324A2 (en) |
AU (1) | AU2001296864A1 (en) |
WO (1) | WO2002018660A2 (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003076641A2 (en) * | 2002-03-11 | 2003-09-18 | Universidad De Sevilla | Method of detecting and quickly identifying pathogenic bacteria that can be transmitted through food using the real-time polymerase chain reaction |
EP1403386A1 (en) * | 2002-09-26 | 2004-03-31 | Mayo Foundation For Medical Education And Research | Detection of Epstein-Barr virus |
US6958210B2 (en) | 2001-01-31 | 2005-10-25 | Mayo Foundation For Medical Education And Research | Detection of herpes simplex virus |
US7074598B2 (en) | 2002-09-25 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of vancomycin-resistant enterococcus spp. |
US7074599B2 (en) | 2002-09-27 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of mecA-containing Staphylococcus spp. |
US7427475B2 (en) | 2003-11-18 | 2008-09-23 | Mayo Foundation For Medical Education And Research | Detection of group B streptococcus |
US7452984B2 (en) | 2000-08-31 | 2008-11-18 | Mayo Foundation For Medical Education And Research | Detection of varicella-zoster virus |
US7691571B2 (en) | 2001-01-31 | 2010-04-06 | Mayo Foundation For Medical Education And Research | Detection of bordetella |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8535888B2 (en) * | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
CA2984820C (en) | 2007-04-04 | 2021-12-07 | Ande Corporation | Plastic microfluidic separation and detection platforms |
US20090226889A1 (en) * | 2008-01-14 | 2009-09-10 | Chen Fan | Methods and compositions for detecting cns viruses |
WO2010017381A2 (en) * | 2008-08-07 | 2010-02-11 | The Regents Of The University Of Colorado, A Body Corporate | Methods for the diagnosis of varicella zoster virus infection |
JP2012529908A (en) * | 2009-06-15 | 2012-11-29 | ネットバイオ・インコーポレーテッド | Improved method for quantification of forensic DNA |
WO2012155084A1 (en) | 2011-05-12 | 2012-11-15 | Netbio, Inc. | Methods and compositions for rapid multiplex amplification of str loci |
US10307082B2 (en) * | 2015-06-02 | 2019-06-04 | Htc Corporation | Method and apparatus for herpes zoster diagnosis and recording medium using the method |
Family Cites Families (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4996143A (en) | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
JP2652959B2 (en) | 1987-12-15 | 1997-09-10 | 東燃料株式会社 | Drugs and methods for detecting varicella-zoster virus infection |
EP0338591A3 (en) | 1988-04-21 | 1991-09-04 | Microprobe Corporation | Nucleic acid extraction method |
US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
US5683896A (en) | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
JPH0549477A (en) | 1991-08-05 | 1993-03-02 | Wakunaga Pharmaceut Co Ltd | Detection of bacteria of the genus staphylococcus |
DE69231853T2 (en) | 1991-11-07 | 2001-09-13 | Nanotronics Inc | HYBRIDIZING POLYNUCLEOTIDS CONJUGED WITH CHROMOPHORES AND FLUOROPHORS TO GENERATE A DONOR-TO-DONOR ENERGY TRANSFER SYSTEM |
JPH07199A (en) | 1993-06-18 | 1995-01-06 | Teijin Ltd | Primer set for detecting herpes zoster varicellosus virus and detection of the virus using the set |
US5702895A (en) | 1995-01-19 | 1997-12-30 | Wakunaga Seiyaku Kabushiki Kaisha | Method and kit for detecting methicillin-resistant Staphylococcus aureus |
US5925733A (en) * | 1995-07-14 | 1999-07-20 | University Of Washington | DNA polymerase of gamma herpes viruses associated with Kaposi's sarcoma and retroperitoneal fibromatosis |
ATE428801T1 (en) * | 1996-06-04 | 2009-05-15 | Univ Utah Res Found | MONITORING HYBRIDIZATION DURING PCR |
DE69738605T2 (en) | 1996-06-04 | 2009-04-30 | University Of Utah Research Foundation, Salt Lake City | Container for carrying out and monitoring biological processes |
EP0994965A2 (en) | 1997-04-22 | 2000-04-26 | Bavarian Nordic Research Institute A/S | Taqman?tm -pcr for the detection of pathogenic e. coli strains |
CA2306443A1 (en) | 1997-10-14 | 1999-04-22 | Darwin Molecular Corporation | Thymidine kinase mutants and fusion proteins having thymidine kinase and guanylate kinase activities |
US6140054A (en) * | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
PL201887B1 (en) | 1998-12-22 | 2009-05-29 | Microscience Ltd | Genes and proteins, and their use |
US6162605A (en) | 1999-04-12 | 2000-12-19 | Becton Dickinson And Company | Amplification and detection of shiga-like toxin I producing organisms |
EP1210365A2 (en) | 1999-08-17 | 2002-06-05 | Beth Israel Deaconess Medical Center, Inc. | Methods and compositions for restoring antibiotic susceptibility in glycopeptide-resistant enterococcus |
EP1246935B1 (en) | 1999-09-28 | 2013-08-14 | Geneohm Sciences Canada Inc. | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
US7297517B2 (en) | 2000-05-29 | 2007-11-20 | Tosoh Corporation | Oligonucleotides and method for detection of meca gene of methicillin-resistant Staphylococcus aureus |
AU2001296864A1 (en) | 2000-08-31 | 2002-03-13 | Mayo Foundation For Medical Education And Research | Detection of varicella-zoster virus |
SG165981A1 (en) | 2000-10-27 | 2010-11-29 | Chiron Srl | Nucleic acids and proteins from streptococcus groups a & b |
US7252937B2 (en) | 2002-02-11 | 2007-08-07 | Auburn University | High-sensitivity real-time polymerase chain reaction for detection of nucleic acids |
US6593093B1 (en) | 2002-02-20 | 2003-07-15 | Mayo Foundation For Medical Education And Research | Detection of group a Streptococcus |
-
2001
- 2001-08-31 AU AU2001296864A patent/AU2001296864A1/en not_active Abandoned
- 2001-08-31 EP EP01977773A patent/EP1360324A2/en not_active Withdrawn
- 2001-08-31 US US09/945,203 patent/US6849407B2/en not_active Expired - Lifetime
- 2001-08-31 WO PCT/US2001/041980 patent/WO2002018660A2/en active Search and Examination
-
2004
- 2004-07-06 US US10/885,437 patent/US7452984B2/en not_active Expired - Lifetime
Non-Patent Citations (9)
Title |
---|
DAVISON A J ET AL: "THE COMPLETE DNA SEQUENCE OF VARICELLA-ZOSTER VIRUS" JOURNAL OF GENERAL VIROLOGY, READING, BERKS, GB, vol. 67, no. 9, 1986, pages 1759-1816, XP001056820 ISSN: 0022-1317 * |
ESPY ET AL: "Diagnosis of Herpes Simplex Virus Infections in the Clinical Laboratory by LightCycler PCR" JOURNAL OF CLINICAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 38, no. 2, February 2000 (2000-02), pages 795-799, XP002175791 ISSN: 0095-1137 cited in the application * |
ESPY M J ET AL: "Diagnosis of varicella-zoster virus infections in the clinical laboratory by LightCycler PCR." JOURNAL OF CLINICAL MICROBIOLOGY. UNITED STATES SEP 2000, vol. 38, no. 9, September 2000 (2000-09), pages 3187-3189, XP002230170 ISSN: 0095-1137 * |
HAWRAMI K ET AL: "Development of a flurogenic polymerase chain reaction assay (TaqMan) for the detection and quantitation of varicella zoster virus." JOURNAL OF VIROLOGICAL METHODS, vol. 79, no. 1, April 1999 (1999-04), pages 33-40, XP002230169 ISSN: 0166-0934 * |
KIMURA HIROSHI ET AL: "Comparison of quantitations of viral load in varicella and zoster." JOURNAL OF CLINICAL MICROBIOLOGY, vol. 38, no. 6, June 2000 (2000-06), pages 2447-2449, XP002230168 ISSN: 0095-1137 * |
KIMURA HIROSHI ET AL: "Recombinant varicella-zoster virus glycoproteins E and I: Immunologic responses and clearance of virus in a guinea pig model of chronic uveitis." JOURNAL OF INFECTIOUS DISEASES, vol. 178, no. 2, August 1998 (1998-08), pages 310-317, XP009005430 ISSN: 0022-1899 * |
PEVENSTEIN STEPHANIE R ET AL: "Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia." JOURNAL OF VIROLOGY, vol. 73, no. 12, December 1999 (1999-12), pages 10514-10518, XP002230165 ISSN: 0022-538X * |
SAUERBREI A ET AL: "Laboratory diagnosis of herpes zoster." JOURNAL OF CLINICAL VIROLOGY: THE OFFICIAL PUBLICATION OF THE PAN AMERICAN SOCIETY FOR CLINICAL VIROLOGY. NETHERLANDS SEP 1999, vol. 14, no. 1, September 1999 (1999-09), pages 31-36, XP002230166 ISSN: 1386-6532 * |
SILVA DE D ET AL: "Rapid Genotyping and Quantification on the LightCycler with Hybridization Probes" BIOCHEMICA, MANNHEIM, DE, no. 2, 1998, pages 12-15, XP002175789 ISSN: 0946-1310 * |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7452984B2 (en) | 2000-08-31 | 2008-11-18 | Mayo Foundation For Medical Education And Research | Detection of varicella-zoster virus |
US8507201B2 (en) | 2001-01-31 | 2013-08-13 | Mayo Foundation For Medical Education And Research | Detection of Bordetella |
US7691571B2 (en) | 2001-01-31 | 2010-04-06 | Mayo Foundation For Medical Education And Research | Detection of bordetella |
US7893241B2 (en) | 2001-01-31 | 2011-02-22 | Mayo Foundation For Medical Education And Research | Articles of manufacture for detection of herpes simplex virus |
US6958210B2 (en) | 2001-01-31 | 2005-10-25 | Mayo Foundation For Medical Education And Research | Detection of herpes simplex virus |
US7667025B2 (en) | 2001-01-31 | 2010-02-23 | Mayo Foundation For Medical Education And Research | Articles of manufacture for detection of herpes simplex virus |
ES2223216A1 (en) * | 2002-03-11 | 2005-02-16 | Universidad De Sevilla | Method of detecting and quickly identifying pathogenic bacteria that can be transmitted through food using the real-time polymerase chain reaction |
WO2003076641A2 (en) * | 2002-03-11 | 2003-09-18 | Universidad De Sevilla | Method of detecting and quickly identifying pathogenic bacteria that can be transmitted through food using the real-time polymerase chain reaction |
WO2003076641A3 (en) * | 2002-03-11 | 2003-12-04 | Univ Sevilla | Method of detecting and quickly identifying pathogenic bacteria that can be transmitted through food using the real-time polymerase chain reaction |
US7074598B2 (en) | 2002-09-25 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of vancomycin-resistant enterococcus spp. |
US7365176B2 (en) | 2002-09-26 | 2008-04-29 | Mayo Foundation For Medical Education And Research | Detection of Epstein-Barr virus |
EP1403386A1 (en) * | 2002-09-26 | 2004-03-31 | Mayo Foundation For Medical Education And Research | Detection of Epstein-Barr virus |
US7074599B2 (en) | 2002-09-27 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of mecA-containing Staphylococcus spp. |
US7427475B2 (en) | 2003-11-18 | 2008-09-23 | Mayo Foundation For Medical Education And Research | Detection of group B streptococcus |
US8097413B2 (en) | 2003-11-18 | 2012-01-17 | Mayo Foundation For Medical Education And Research | Detection of group B Streptococcus |
US7790875B2 (en) | 2003-11-18 | 2010-09-07 | Mayo Foundation For Medical Education And Research | Detection of group B Streptococcus |
Also Published As
Publication number | Publication date |
---|---|
US7452984B2 (en) | 2008-11-18 |
US20020182634A1 (en) | 2002-12-05 |
US20040259078A1 (en) | 2004-12-23 |
WO2002018660A3 (en) | 2003-08-14 |
AU2001296864A1 (en) | 2002-03-13 |
EP1360324A2 (en) | 2003-11-12 |
WO2002018660A9 (en) | 2003-05-08 |
US6849407B2 (en) | 2005-02-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7667025B2 (en) | Articles of manufacture for detection of herpes simplex virus | |
US6849407B2 (en) | Detection of varicella-zoster virus | |
EP2252702A2 (en) | Detection of clostridium difficile | |
US7615352B2 (en) | Detection of Epstein-Barr virus | |
US7074598B2 (en) | Detection of vancomycin-resistant enterococcus spp. | |
US20030165866A1 (en) | Detection of bordetella | |
US20070238093A1 (en) | Detection of influenza A virus | |
EP4004240B1 (en) | Compositions and methods for detection of epstein barr virus (ebv) | |
CA2648949C (en) | Detection of influenza a virus | |
US7427475B2 (en) | Detection of group B streptococcus | |
EP1308524B1 (en) | Detection of variola virus | |
WO2024003260A1 (en) | Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis | |
US20110045458A1 (en) | Detection of Enterovirus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2001977773 Country of ref document: EP |
|
COP | Corrected version of pamphlet |
Free format text: PAGES 25-31, CLAIMS, REPLACED BY NEW PAGES 25-31; AFTER RECTIFICATION OF OBVIOUS ERRORS AS AUTHORIZED BY THE INTERNATIONAL SEARCHING AUTHORITY |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 2001977773 Country of ref document: EP |
|
NENP | Non-entry into the national phase |
Ref country code: JP |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 2001977773 Country of ref document: EP |
|
DPE2 | Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101) |