WO2002022869A3 - Directed evolution method - Google Patents
Directed evolution method Download PDFInfo
- Publication number
- WO2002022869A3 WO2002022869A3 PCT/GB2001/004108 GB0104108W WO0222869A3 WO 2002022869 A3 WO2002022869 A3 WO 2002022869A3 GB 0104108 W GB0104108 W GB 0104108W WO 0222869 A3 WO0222869 A3 WO 0222869A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- replicase
- nucleic acid
- pool
- selecting
- acid member
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Abstract
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE60124272T DE60124272T2 (en) | 2000-09-13 | 2001-09-13 | METHOD FOR TARGETED EVOLUTION |
AU2001286103A AU2001286103B2 (en) | 2000-09-13 | 2001-09-13 | Directed evolution method |
EP01965462A EP1317539B1 (en) | 2000-09-13 | 2001-09-13 | Directed evolution method |
DK01965462T DK1317539T3 (en) | 2000-09-13 | 2001-09-13 | Approach to Targeted Evolution |
AU8610301A AU8610301A (en) | 2000-09-13 | 2001-09-13 | Directed evolution method |
CA2421963A CA2421963C (en) | 2000-09-13 | 2001-09-13 | Directed evolution method |
JP2002527309A JP5009481B2 (en) | 2000-09-13 | 2001-09-13 | Directional evolution method |
US10/387,387 US7514210B2 (en) | 2000-09-13 | 2003-03-13 | Compartmentalised self replication method for in vitro evolution of molecular libraries |
US12/070,232 US20080166772A1 (en) | 2000-09-13 | 2008-02-15 | Methods of increasing the concentration of a nucleic acid |
US12/392,412 US20090246853A1 (en) | 2000-09-13 | 2009-02-25 | Directed evolution method |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0022458.4A GB0022458D0 (en) | 2000-09-13 | 2000-09-13 | Directed evolution method |
GB0022458.4 | 2000-09-13 | ||
US28377101P | 2001-04-13 | 2001-04-13 | |
US60/283,771 | 2001-04-13 | ||
US28550101P | 2001-04-20 | 2001-04-20 | |
US60/285,501 | 2001-04-20 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/387,387 Continuation US7514210B2 (en) | 2000-09-13 | 2003-03-13 | Compartmentalised self replication method for in vitro evolution of molecular libraries |
US10/387,387 Continuation-In-Part US7514210B2 (en) | 2000-09-13 | 2003-03-13 | Compartmentalised self replication method for in vitro evolution of molecular libraries |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002022869A2 WO2002022869A2 (en) | 2002-03-21 |
WO2002022869A3 true WO2002022869A3 (en) | 2002-10-17 |
Family
ID=27255886
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2001/004108 WO2002022869A2 (en) | 2000-09-13 | 2001-09-13 | Directed evolution method |
Country Status (11)
Country | Link |
---|---|
US (3) | US7514210B2 (en) |
EP (4) | EP1806406A3 (en) |
JP (2) | JP5009481B2 (en) |
AT (2) | ATE396259T1 (en) |
AU (2) | AU2001286103B2 (en) |
CA (2) | CA2585083A1 (en) |
DE (2) | DE60134181D1 (en) |
DK (1) | DK1317539T3 (en) |
ES (2) | ES2274901T3 (en) |
GB (1) | GB0022458D0 (en) |
WO (1) | WO2002022869A2 (en) |
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US8871444B2 (en) | 2004-10-08 | 2014-10-28 | Medical Research Council | In vitro evolution in microfluidic systems |
US9012390B2 (en) | 2006-08-07 | 2015-04-21 | Raindance Technologies, Inc. | Fluorocarbon emulsion stabilizing surfactants |
US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
US9228229B2 (en) | 2010-02-12 | 2016-01-05 | Raindance Technologies, Inc. | Digital analyte analysis |
US9273308B2 (en) | 2006-05-11 | 2016-03-01 | Raindance Technologies, Inc. | Selection of compartmentalized screening method |
US9328344B2 (en) | 2006-01-11 | 2016-05-03 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
US9364803B2 (en) | 2011-02-11 | 2016-06-14 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
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US9448172B2 (en) | 2003-03-31 | 2016-09-20 | Medical Research Council | Selection by compartmentalised screening |
US9498759B2 (en) | 2004-10-12 | 2016-11-22 | President And Fellows Of Harvard College | Compartmentalized screening by microfluidic control |
US9562897B2 (en) | 2010-09-30 | 2017-02-07 | Raindance Technologies, Inc. | Sandwich assays in droplets |
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GB9900298D0 (en) * | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
FR2843404A1 (en) * | 2002-08-07 | 2004-02-13 | Cellectis | Testing cleavage activity of endonucleases, useful as reagents in molecular biology and genomic engineering, that generate overhangs, comprises testing their effects on duplex DNA substrates |
AU2004254552B2 (en) | 2003-01-29 | 2008-04-24 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
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US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
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- 2001-09-13 AU AU2001286103A patent/AU2001286103B2/en not_active Expired
- 2001-09-13 JP JP2002527309A patent/JP5009481B2/en not_active Expired - Lifetime
- 2001-09-13 EP EP06026714A patent/EP1806406A3/en not_active Withdrawn
- 2001-09-13 ES ES01965462T patent/ES2274901T3/en not_active Expired - Lifetime
- 2001-09-13 ES ES04078077T patent/ES2304580T3/en not_active Expired - Lifetime
- 2001-09-13 EP EP08103279A patent/EP1964932A3/en not_active Withdrawn
- 2001-09-13 WO PCT/GB2001/004108 patent/WO2002022869A2/en active IP Right Grant
- 2001-09-13 DE DE60134181T patent/DE60134181D1/en not_active Expired - Lifetime
- 2001-09-13 DK DK01965462T patent/DK1317539T3/en active
- 2001-09-13 AT AT04078077T patent/ATE396259T1/en not_active IP Right Cessation
- 2001-09-13 AU AU8610301A patent/AU8610301A/en active Pending
- 2001-09-13 EP EP04078077A patent/EP1505151B1/en not_active Expired - Lifetime
- 2001-09-13 EP EP01965462A patent/EP1317539B1/en not_active Expired - Lifetime
- 2001-09-13 DE DE60124272T patent/DE60124272T2/en not_active Expired - Lifetime
- 2001-09-13 CA CA2421963A patent/CA2421963C/en not_active Expired - Lifetime
- 2001-09-13 AT AT01965462T patent/ATE344320T1/en not_active IP Right Cessation
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US20040005594A1 (en) | 2004-01-08 |
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CA2421963C (en) | 2013-04-09 |
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