WO2002031112A3 - Enhanced transformation and regeneration of transformed embryogenic pine tissue - Google Patents

Enhanced transformation and regeneration of transformed embryogenic pine tissue Download PDF

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Publication number
WO2002031112A3
WO2002031112A3 PCT/US2001/031586 US0131586W WO0231112A3 WO 2002031112 A3 WO2002031112 A3 WO 2002031112A3 US 0131586 W US0131586 W US 0131586W WO 0231112 A3 WO0231112 A3 WO 0231112A3
Authority
WO
WIPO (PCT)
Prior art keywords
regeneration
transformed embryogenic
transformation
enhanced transformation
embryogenic tissue
Prior art date
Application number
PCT/US2001/031586
Other languages
French (fr)
Other versions
WO2002031112A2 (en
Inventor
Marie Bernice Connett-Porceddu
Heather Jeanne Gladfelter
Jon Eric Gulledge
Ryan R Mccormack
Original Assignee
Westvaco Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Westvaco Corp filed Critical Westvaco Corp
Priority to AU2002213081A priority Critical patent/AU2002213081B2/en
Priority to CA2424313A priority patent/CA2424313C/en
Priority to NZ525632A priority patent/NZ525632A/en
Priority to AU1308102A priority patent/AU1308102A/en
Priority to BR0114563-0A priority patent/BR0114563A/en
Publication of WO2002031112A2 publication Critical patent/WO2002031112A2/en
Publication of WO2002031112A3 publication Critical patent/WO2002031112A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Abstract

The present invention relates to methods for the transformation and regeneration of transformed embryogenic tissue of coniferous plants. In particular, the invention relates to improved methods for transforming embryogenic tissue of coniferous plants and for regenerating transformed embryogenic tissue of coniferous plants. The invention is well suited to the transformation and regeneration of transformed embryogenic tissue of plants of the subgenus Pinus of pines and hybrids thereof.
PCT/US2001/031586 2000-10-10 2001-10-10 Enhanced transformation and regeneration of transformed embryogenic pine tissue WO2002031112A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2002213081A AU2002213081B2 (en) 2000-10-10 2001-10-10 Enhanced transformation and regeneration of transformed embryogenic pine tissue
CA2424313A CA2424313C (en) 2000-10-10 2001-10-10 Enhanced transformation and regeneration of transformed embryogenic pine tissue
NZ525632A NZ525632A (en) 2000-10-10 2001-10-10 Transformation and regeneration of transformed embryogenic pine tissue using agrobacterium
AU1308102A AU1308102A (en) 2000-10-10 2001-10-10 Enhanced transformation and regeneration of transformed embryogenic pine tissue
BR0114563-0A BR0114563A (en) 2000-10-10 2001-10-10 Improved transformation and regeneration of transformed embryogenic pine tissue

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US23914300P 2000-10-10 2000-10-10
US60/239,143 2000-10-10
US29726701P 2001-06-12 2001-06-12
US60/297,267 2001-06-12

Publications (2)

Publication Number Publication Date
WO2002031112A2 WO2002031112A2 (en) 2002-04-18
WO2002031112A3 true WO2002031112A3 (en) 2002-07-25

Family

ID=26932312

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2001/031586 WO2002031112A2 (en) 2000-10-10 2001-10-10 Enhanced transformation and regeneration of transformed embryogenic pine tissue
PCT/US2001/031588 WO2002031113A2 (en) 2000-10-10 2001-10-10 Enhanced selection of genetically modified pine embryogenic tissue

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2001/031588 WO2002031113A2 (en) 2000-10-10 2001-10-10 Enhanced selection of genetically modified pine embryogenic tissue

Country Status (7)

Country Link
US (2) US7157620B2 (en)
AR (2) AR030875A1 (en)
AU (4) AU1456602A (en)
BR (1) BR0114563A (en)
CA (3) CA2424314C (en)
NZ (2) NZ525633A (en)
WO (2) WO2002031112A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7598073B2 (en) 2002-04-09 2009-10-06 Weyerhaeuser Nr Company Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose
US7381562B2 (en) 2002-05-30 2008-06-03 Weyerhaeuser Company Methods for producing cotyledonary pine embryos utilizing a gibberellin
CA2435337C (en) * 2002-11-14 2010-03-23 Weyerhaeuser Company Methods for producing conifer somatic embryos
NZ544386A (en) * 2003-06-06 2010-07-30 Arborgen Llc Polypeptides with cellulose synthase GDP forming activity
NZ544391A (en) 2003-06-06 2009-06-26 Arborgen Llc Plant transformation and selection
CA2528536A1 (en) 2003-06-06 2005-01-06 Arborgen, Llc. Transcription factors
US7521237B2 (en) * 2003-06-23 2009-04-21 Weyerhaeuser Nr Company Methods for promoting maturation of conifer somatic embryos
US20040266002A1 (en) * 2003-06-30 2004-12-30 Weyerhaeuser Company Use of abscisic acid in somatic embryogenesis of pine trees
US7732205B2 (en) * 2003-07-30 2010-06-08 Weyerhaeuser Nr Company Development and stratification of pine somatic embryos using a liquid system
US7520089B2 (en) * 2003-11-25 2009-04-21 Weyerhaeuser Nr Company Method to improve manufactured seed germination
AR047574A1 (en) * 2003-12-30 2006-01-25 Arborgen Llc 2 Genesis Res 1 CELL CYCLE GENES AND RELATED USE METHODS
CA2495650C (en) * 2004-02-25 2011-05-24 Weyerhaeuser Company Continuous culture of conifer embryogenic tissue
US7452722B2 (en) * 2004-03-02 2008-11-18 Weyerhaeuser Company Methods for developing conifer somatic embryos
US7897838B2 (en) * 2004-04-20 2011-03-01 Temasek Life Sciences Laboratory Limited Methods for high efficiency transformation and regeneration of plant suspension cultures
US7799906B1 (en) 2004-09-22 2010-09-21 Arborgen, Llc Compositions and methods for modulating lignin of a plant
US7402428B2 (en) 2004-09-22 2008-07-22 Arborgen, Llc Modification of plant lignin content
US7453025B2 (en) * 2004-09-22 2008-11-18 Arborgen, Llc Reproductive ablation constructs
US7456338B2 (en) 2004-09-22 2008-11-25 Arborgen Llc Modification of plant lignin content
US20060101536A1 (en) * 2004-11-05 2006-05-11 Arborgen, Llc Eucalyptus urophylla transformation and regeneration
US20070099293A1 (en) * 2005-10-27 2007-05-03 Weyerhaeuser Co. Use of porous membrane to support developing conifer somatic embryos
CA2568476C (en) * 2005-12-22 2015-03-17 Weyerhaeuser Company Methods for storing conifer somatic embryo germinants
US7785884B2 (en) * 2006-09-28 2010-08-31 Weyerhaeuser Nr Company Low density spreading methods for conifer somatic embryogenesis
US8338674B2 (en) * 2010-05-10 2012-12-25 Monsanto Technology Llc Plants and seeds of corn variety CV289398
BR112013015247B1 (en) * 2010-12-17 2021-11-23 Monsanto Technology Llc METHOD TO IMPROVE THE COMPETENCE OF CORN CELLS FOR BACTERIA-MEDIATED TRANSFORMATION
CN106035087B (en) * 2016-06-16 2017-11-28 杭州师范大学 A kind of method of Changshan grapefruit fast asexual propagation
CN109874675A (en) * 2019-04-09 2019-06-14 南京林业大学 A method of promote China fir body embryo to occur using deionized formamide
US10964372B2 (en) 2019-06-14 2021-03-30 Micron Technology, Inc. Memory cell biasing techniques

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5491090A (en) * 1994-02-09 1996-02-13 Westvaco Corporation Embryogenic coniferous liquid suspension cultures

Family Cites Families (6)

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US5187092A (en) 1990-03-22 1993-02-16 Institute Of Paper Science And Technology, Inc. Somatic embryogenesis in gymnosperms
US6340594B1 (en) 1991-12-19 2002-01-22 Cellfor, Inc. Production of desiccation-tolerant gymnosperm embryos
US5677185A (en) * 1996-05-14 1997-10-14 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis in culture media containing abscisic acid
US5850020A (en) 1996-09-11 1998-12-15 Genesis Research & Development Corporation, Ltd. Materials and method for the modification of plant lignin content
US5731204A (en) * 1996-12-20 1998-03-24 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis employing polyethylene glycol
WO1999046977A1 (en) 1998-03-17 1999-09-23 Silvagen Inc. Maturation of somatic embryos

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5491090A (en) * 1994-02-09 1996-02-13 Westvaco Corporation Embryogenic coniferous liquid suspension cultures

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ATTREE S.M.: "Somatic embryo maturation, germination and soil establishment of plants of black and white spruce (Picea mariana and picea glauca)", CAN. J. BOT., vol. 68, 1990, pages 2583 - 2589, XP002909585 *
WEI T.: "Genetic transformation of loblolly pine using mature zygotic embryo explants by agrobacterium tumefaciens", JOURNAL OF FORESTRY RESEARCH, vol. 11, no. 4, 2000, pages 215 - 222, XP002909584 *

Also Published As

Publication number Publication date
AU1456602A (en) 2002-04-22
WO2002031113A3 (en) 2002-07-04
NZ525633A (en) 2005-07-29
AU2002214566B2 (en) 2007-11-22
CA2424313C (en) 2010-08-17
AR030876A1 (en) 2003-09-03
CA2424314A1 (en) 2002-04-18
CA2424314C (en) 2013-04-16
US6964870B2 (en) 2005-11-15
WO2002031113A2 (en) 2002-04-18
BR0114563A (en) 2004-01-20
AU2002213081B2 (en) 2006-12-07
CA2424313A1 (en) 2002-04-18
AU1308102A (en) 2002-04-22
US7157620B2 (en) 2007-01-02
US20020100083A1 (en) 2002-07-25
US20020083495A1 (en) 2002-06-27
WO2002031112A2 (en) 2002-04-18
NZ525632A (en) 2004-09-24
CA2705037A1 (en) 2002-04-18
AR030875A1 (en) 2003-09-03

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