WO2002036832A3 - Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis - Google Patents

Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis Download PDF

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Publication number
WO2002036832A3
WO2002036832A3 PCT/US2001/048654 US0148654W WO0236832A3 WO 2002036832 A3 WO2002036832 A3 WO 2002036832A3 US 0148654 W US0148654 W US 0148654W WO 0236832 A3 WO0236832 A3 WO 0236832A3
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WO
WIPO (PCT)
Prior art keywords
fluorescent dyes
atropisomers
dna sequencing
methods
fragment analysis
Prior art date
Application number
PCT/US2001/048654
Other languages
French (fr)
Other versions
WO2002036832A2 (en
Inventor
Linda G Lee
Meng C Taing
Barnett B Rosemblum
Original Assignee
Pe Corp Ny
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pe Corp Ny filed Critical Pe Corp Ny
Priority to AU2002230914A priority Critical patent/AU2002230914A1/en
Priority to JP2002539575A priority patent/JP2004532805A/en
Priority to CA002426121A priority patent/CA2426121A1/en
Priority to EP01991171A priority patent/EP1330550A2/en
Publication of WO2002036832A2 publication Critical patent/WO2002036832A2/en
Publication of WO2002036832A3 publication Critical patent/WO2002036832A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Abstract

Substantially pure atropisomers of xanthene compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
PCT/US2001/048654 2000-11-01 2001-10-30 Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis WO2002036832A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002230914A AU2002230914A1 (en) 2000-11-01 2001-10-30 Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
JP2002539575A JP2004532805A (en) 2000-11-01 2001-10-30 Methods for analysis of atropisomers and DNA sequencing fragments of asymmetric xanthene fluorescein dyes
CA002426121A CA2426121A1 (en) 2000-11-01 2001-10-30 Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis
EP01991171A EP1330550A2 (en) 2000-11-01 2001-10-30 Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/704,966 US6448407B1 (en) 2000-11-01 2000-11-01 Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
US09/704,966 2000-11-01

Publications (2)

Publication Number Publication Date
WO2002036832A2 WO2002036832A2 (en) 2002-05-10
WO2002036832A3 true WO2002036832A3 (en) 2002-08-01

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PCT/US2001/048654 WO2002036832A2 (en) 2000-11-01 2001-10-30 Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis

Country Status (6)

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US (5) US6448407B1 (en)
EP (1) EP1330550A2 (en)
JP (1) JP2004532805A (en)
AU (1) AU2002230914A1 (en)
CA (1) CA2426121A1 (en)
WO (1) WO2002036832A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6372907B1 (en) * 1999-11-03 2002-04-16 Apptera Corporation Water-soluble rhodamine dye peptide conjugates
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US20040081959A9 (en) * 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6448407B1 (en) * 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
JP2004537263A (en) * 2000-12-14 2004-12-16 ジェン−プローブ・インコーポレーテッド Methods and kits for enhancing the rate of polynucleotide association
JP2005532434A (en) * 2002-07-01 2005-10-27 グアヴァ テクノロジーズ，インコーポレーテッド Fluorescent dyes, energy transfer pairs and methods
US7109316B2 (en) * 2002-08-23 2006-09-19 Ce Healthcare Bio-Sciences Corp. Oligonucleotide tagged nucleoside triphosphates (OTNTPs) for genetic analysis
US7803536B2 (en) * 2002-09-20 2010-09-28 Integrated Dna Technologies, Inc. Methods of detecting fluorescence with anthraquinone quencher dyes
EP1606419A1 (en) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
WO2004092330A2 (en) * 2003-04-11 2004-10-28 Applera Corporation Method of generating long nucleic acid molecules of defined sequence
US20050095610A1 (en) * 2003-04-11 2005-05-05 Kuo Sophia S. Method of generating size standard nucleic acids
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20050202484A1 (en) 2004-02-19 2005-09-15 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
US7767834B2 (en) * 2004-08-13 2010-08-03 Elitech Holding B.V. Phosphonylated fluorescent dyes and conjugates
ES2461858T3 (en) 2004-08-13 2014-05-21 Epoch Biosciences, Inc. Phosphonate and conjugate fluorescent dyes
CA2601554A1 (en) 2005-05-20 2006-11-30 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US8178360B2 (en) * 2006-05-18 2012-05-15 Illumina Cambridge Limited Dye compounds and the use of their labelled conjugates
WO2008011508A1 (en) * 2006-07-21 2008-01-24 Board Of Supervisors Of Louisiana State Universityand Agricultural And Mechanical College Fluorescent xanthenes and white light fluorophores
JP5360647B2 (en) * 2006-12-19 2013-12-04 独立行政法人理化学研究所 Fluorogenic molecule
US8698031B2 (en) * 2007-04-17 2014-04-15 Dynamic Connections, Llc Separation and manipulation of a chiral object
WO2008153223A1 (en) 2007-07-31 2008-12-18 Osaka University Composition for measuring the binding affinity between nucleic acid and substance, and use thereof
US9273022B2 (en) 2009-09-18 2016-03-01 Provectus Pharmaceuticals, Inc. Process for the synthesis of 4,5,6,7-tetrachloro-3′,6′-dihydroxy-2′, 4′, 5′7′-tetraiodo-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (Rose Bengal) and related xanthenes
CA2771988C (en) 2009-09-18 2016-07-05 Provectus Pharmaceuticals, Inc. Process for the synthesis of 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodo-3h-spiro[isobenzofuran-1,9'-xanthen]-3-one (rose bengal) and related xanthenes
US20120108799A1 (en) 2010-09-07 2012-05-03 Integrated Dna Technologies, Inc. Modifications for Antisense Compounds
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
AU2011230496B2 (en) 2010-03-26 2015-09-17 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
CN104470991A (en) * 2012-05-30 2015-03-25 生命科技公司 Fluorogenic pH-sensitive dyes and their methods of use
TWI468469B (en) 2012-06-22 2015-01-11 Ind Tech Res Inst Dyes and methods of marking biological materials
US9540699B2 (en) 2013-03-13 2017-01-10 William Beaumont Hospital Methods of diagnosing increased risk of developing MRSA
EP3019559A4 (en) 2013-08-22 2017-04-05 Sony Corporation Water soluble fluorescent or colored dyes and methods for their use
WO2015109136A2 (en) 2014-01-16 2015-07-23 Sony Corporation Water soluble fluorescent or colored dyes and methods for their use
US9840340B2 (en) * 2014-12-09 2017-12-12 Dan O'Toole Drone docking station and delivery system
EP3262022A4 (en) 2015-02-26 2018-08-15 Sony Corporation Phenylethynylnaphthalene dyes and methods for their use
CN107454903B (en) * 2015-02-26 2021-01-01 索尼公司 Water-soluble fluorescent or colored dyes containing conjugated groups
CN104910229B (en) * 2015-04-30 2019-11-12 赛纳生物科技(北京)有限公司 Polyphosphoric acids Terminal fluorescent labels nucleotide and its application
US10865310B2 (en) 2015-05-11 2020-12-15 Sony Corporation Of America Ultra bright dimeric or polymeric dyes
US11434377B2 (en) 2016-04-01 2022-09-06 Sony Corporation Ultra bright dimeric or polymeric dyes with rigid spacing groups
CA3018564A1 (en) 2016-04-01 2017-10-05 Sony Corporation Ultra bright dimeric or polymeric fluorescent and colored dyes
AU2017248165B2 (en) 2016-04-06 2021-08-26 Sony Group Corporation Ultra bright dimeric or polymeric dyes with spacing linker groups
JP2019519490A (en) 2016-05-10 2019-07-11 ソニー株式会社 Ultra bright polymer dye having a peptide backbone
JP7068192B2 (en) 2016-05-10 2022-05-16 ソニーグループ株式会社 Compositions containing polymer dyes and cyclodextrins, and their use
CN109071961B (en) 2016-05-11 2021-04-27 索尼公司 Ultra-bright dimeric or polymeric dyes
US11377563B2 (en) 2016-06-06 2022-07-05 Sony Corporation Ionic polymers comprising fluorescent or colored reporter groups
CN107417670B (en) * 2017-07-08 2019-06-04 常州大学 A kind of near-infrared squarylium cyanine dyes probe and its preparation method and application based on thymidine
EP3691690A1 (en) 2017-10-05 2020-08-12 Sony Corporation Programmable polymeric drugs
EP3710062A1 (en) 2017-11-16 2020-09-23 Sony Corporation Programmable polymeric drugs
CN108344722B (en) * 2018-02-09 2020-08-21 赣南师范大学 Application of near-infrared xanthene fluorescent dye in schistosome adult fluorescent labeling
WO2019182765A1 (en) 2018-03-19 2019-09-26 Sony Corporation Use of divalent metals for enhancement of fluorescent signals
US20210032474A1 (en) 2018-03-21 2021-02-04 Sony Corporation Polymeric tandem dyes with linker groups
CN109741798B (en) * 2018-12-25 2022-03-15 深圳晶泰科技有限公司 Automatic production method of stereoisomeric complete set of organic molecules
WO2021062176A2 (en) 2019-09-26 2021-04-01 Sony Corporation Polymeric tandem dyes with linker groups
EP4034131A4 (en) * 2019-09-27 2023-10-18 Brentano Biotechnology Associates Diastereomeric linking reagents for nucleotide probes
CN111410624B (en) * 2020-01-09 2022-05-06 江西科技师范大学 Asymmetric naphthalene-pyrrole hybrid diarylethene compound and application thereof
EP4185858A2 (en) 2020-07-23 2023-05-31 Life Technologies Corporation Compositions, systems and methods for biological analysis using dyes
US20240060117A1 (en) 2020-07-23 2024-02-22 Life Technologies Corporation Energy transfer dye conjugates for use in biological assays
CN113563360A (en) * 2021-08-16 2021-10-29 中国科学技术大学 Amphiphilic fluorescent dye and synthetic method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4510251A (en) * 1982-11-08 1985-04-09 Abbott Laboratories Fluorescent polarization assay for ligands using aminomethylfluorescein derivatives as tracers
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0201751A2 (en) 1985-05-08 1986-11-20 Abbott Laboratories 4'-aminomethyfluorescein derivatives for use in fluorescence polarization immunoassys
EP0232736A1 (en) 1986-02-06 1987-08-19 Abbott Laboratories Preparation of 4'-aminomethylfluorescein derivatives for use in fluorescence polarization immunoassays
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5847162A (en) 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US6448407B1 (en) * 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4510251A (en) * 1982-11-08 1985-04-09 Abbott Laboratories Fluorescent polarization assay for ligands using aminomethylfluorescein derivatives as tracers
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
B.B.ROSENBLUM ET AL.: "NEW DYE-LABELED TERMINATORS FOR IMPROVED DNA SEQUENCING PATTERNS", NUCLEIC ACIDS RESEARCH, vol. 25, no. 22, 1997, OXFORD GB, pages 4500 - 4504, XP002201149 *
LEE L G ET AL: "NEW ENERGY TRANSFER DYES FOR DNA SEQUENCING", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 14, 1997, pages 2816 - 2822, XP002919606, ISSN: 0305-1048 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides

Also Published As

Publication number Publication date
US20030055243A1 (en) 2003-03-20
US6448407B1 (en) 2002-09-10
CA2426121A1 (en) 2002-05-10
AU2002230914A1 (en) 2002-05-15
US6649769B2 (en) 2003-11-18
US20070254298A1 (en) 2007-11-01
US20060188915A1 (en) 2006-08-24
US20040229235A1 (en) 2004-11-18
JP2004532805A (en) 2004-10-28
EP1330550A2 (en) 2003-07-30
US7038063B2 (en) 2006-05-02
WO2002036832A2 (en) 2002-05-10

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